Genscript - Protein Services

Instructions

1. Please complete all the following questions and email this form to .Our service representative will contact you with a quote shortly.
2. This form is designed for E.coli, yeast and insect systems. If you would like to choose mammalian system, please fill out the mammalian quote request form at the bottom of the webpage:

Customer Information

Name:
Phone:
Institution:
Shipping address(needed to determine shipping cost):
Email:

Protein Expression and Purification Information

Protein name:
Protein sequence (crucial for evaluation) oraccession number:
If mutants are included in your protein sequence, please enter the protein sequence.
Do you want GenScript to synthesize and clone the gene for you? Yes No
Do you want GenScript to subclone your gene into our expression constructs? Yes No
If NO, please give a brief description of your expression construct:
Please indicate the preferred expression host system: E.coli Yeast Baculovirus/insect cells
Please indicate culture volume required (in liters):
Is your protein: Membrane-bound Secreted A protease Other enzyme Toxic to E.coli Other features that may cause difficulty in expression or purification (specify):
GenScript will select 1 or 2 tags for you (e.g. 6xHis, GST, or MBP) to facilitate purification
OK No, I want to select on my own (specify):
GenScript may select additional 1 or 2 tags for you (e.g.Trx, TF,Dsb, or Nus) to increase solubility and/or expression level (recommended – can greatly improve yield; the tag will be added to N-terminus)
OK No, I want to select on my own (specify):
Default location of the tag(s) will be at the N-terminus. OK No,I want to select on my own (specify):
(if internal, please use position numbers as in the sequence that corresponds to the accession number you listed above)
Do you want Genscript to add a protease cleavage site between the tags and target protein? Yes No
If YES, which protease should we add:Genscript’s choice Enterokinase PreScission rTev Thrombin
Notes: There will be extra 4-6aa left after protease cleavage of C-terminal tag. So we recommend NOT to introduce protease cleavage site between target protein and C-terminal tag.
Do you want GenScript to remove tag(s)? Yes No Small tag(e.g. His)can be retained after purification
Notes: Tag removal starts from $1500. Besides, the recovery rate varies with the characteristics of the protein.
If the expressed protein is insoluble, do you want Genscript to attempt the refolding? OK No
Notes: Genscript will not charge extra fee for conventional refolding.
Would you like Genscript to follow any specific expression and purification protocol? Yes No
If yes, please attach your protocol here:
Special requirements (buffers, handling, etc.):