Genome-wide identification and characterization of aquaporin gene family in moso bamboo(Phyllostachysedulis)

Huayu Sun1,2* · Lichao Li1,2* ·Yongfeng Lou1,2· Hansheng Zhao1,2· Zhimin Gao1,2,

Huayu Sun

Lichao Li

Yongfeng Lou

Hansheng Zhao

ZhiminGao

1State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China

2InstituteofGeneSciencefor BambooandRattanResources, International Center for Bamboo and Rattan, Beijing 100102, China

*These authors contributed equally to this work

 Corresponding author

SupplementaryTable 1Primers used forqRT-PCR expression analysis

Nomenclature
used for this paper / BambooGDB
assembly name / GenBank
accession number / Primer / Tm (ºC) / PCR
efficiency values / Correlation
coefficients(R2) / Product size(bp)
PePIP1;1 / PH01000000G6020 / FP099961.1 / F: 5′-GATGCCAATCCAAAGAGCAAGC-3′
R: 5′-GACAGAGCAGTACAGCAAACTTGAAG-3′ / 64 / 0.95 / 0.999 / 108
PePIP1;2 / PH01000272G0290 / - / F: 5′-CGGCTACACCAAGGGCGAC-3′
R: 5′-TGGACCAGGAACACTGCGAAC-3′ / 64 / 0.97 / 0.997 / 159
PePIP1;3 / PH01000788G0340 / FP097691.1 / F: 5′-CGAAATGGAGTACGTACCGTTGTT-3′
R: 5′-CACGCACGTACGTACGTTCAGTAG-3′ / 64 / 0.96 / 0.998 / 147
PePIP2;1 / PH01001060G0550 / - / F: 5′-TGTTCATGGTCCACTTGGCG-3′
R: 5′-GATGATAGGGCCGACCCAGA-3′ / 65 / 1.04 / 0.997 / 137
PePIP2;2 / PH01002412G0140 / FP099351.1 / F: 5′-CCAGGCTAGGTGTGGTAGCTAGG-3′
R: 5′-GTACAGCGACCATGACCCCAG-3′ / 64 / 1.04 / 0.990 / 145
PePIP2;3 / PH01003141G0240 / FP096147.1 / F: 5′-AGTACAACAAGGCCGCCTCC-3′
R: 5′-GGGAGACCATTGCACGAGAAG-3′ / 63 / 1.05 / 0.994 / 141
PePIP2;4 / PH01000001G4670 / FP097850.1 / F: 5′-TAGGCGATGGCGAAGGACAT-3′
R: 5′-ACAGGGACCACTTGGTCAGCTC-3′ / 61 / 0.99 / 0.995 / 122
PePIP2;5 / PH01000929G0130 / - / F: 5′-AAGGAGCTAGAGGTGAGCGAGG-3′
R: 5′-TGTAGAGGAACAGAAGCGTGGC-3′ / 62 / 0.96 / 0.995 / 157
PePIP2;6 / PH01000450G0080 / FP095552.1 / F: 5′-ACTGAGCTCGTCAACGGTCAAC-3′
R: 5′-CAAATCATGCACCTGGCGTC-3′ / 62 / 0.98 / 0.991 / 161
PePIP2;7 / PH01000332G0830 / - / F: 5′-CTCCACCATGGACAGGTGCA-3′
R: 5′-AGCTTCCTCGCCACGAACAG-3′ / 60 / 0.96 / 0.992 / 153
PeTIP1;1 / PH01000157G0460 / FP094394.1 / F: 5′-TAGCTAGCTACCCGGCAGAGTCT-3′
R: 5′-TGGCCTGCGAAGACGAAGAT-3′ / 62 / 1.05 / 1.000 / 165
PeTIP1;2 / PH01001117G0550 / FP098066.1 / F: 5′-TCGATCCATCCATTACTCCTCCT-3′
R: 5′-CATCGCCAACAAACAGATGACA-3′ / 62 / 1.05 / 1.000 / 160
PeTIP2;1 / PH01000023G0610 / - / F: 5′-ATGGCGATTGGCTTCATCGT-3′
R: 5′-TCAGTGGCCCGACCCAGTAC-3′ / 60 / 1.01 / 0.999 / 145
PeTIP2;2 / PH01001207G0140 / - / F: 5′-GGTCCATGAACCCAGCAAGGT-3′
R: 5′-CATGAAGACGTCCCCGTAGACT-3′ / 60 / 1.05 / 1.000 / 131
PeTIP4;1 / PH01000003G3730 / FP096242.1 / F: 5′-GTCCTTCTAGTAATGAGCACCACACAT-3′
R: 5′-GAAACAGCTCCTGCAGGCCT-3′ / 64 / 1.05 / 0.999 / 162
Nomenclature
used for this paper / BambooGDB
assembly name / GenBank
accession number / Primer / Tm (℃) / PCR
efficiency values / Correlation
coefficients (R2) / Product size(bp)
PeTIP4;2 / PH01001821G0300 / FP098139.1 / F: 5′-TGACGACAGATCAGCGACACTG-3′
R: 5′-AAGAGGAAGGTGAGCACGAGCT-3′ / 62 / 0.95 / 0.993 / 165
PeNIP1;1 / PH01000253G1030 / FP095251.1 / F: 5′-AGCTCATGAGCAGAGAGCCATG-3′
R: 5′-TTCTTGCTCTGGTTGATCGTCAC-3′ / 64 / 1.05 / 0.991 / 168
PeNIP1;2 / PH01002816G0020 / - / F: 5′-TGTGCTAATCGCAGGGCCTATC-3′
R: 5′-CTTGTTGGTGAACCGGATGAGG-3′ / 66 / 1.03 / 0.995 / 169
PeNIP1;3 / PH01001769G0230 / - / F: 5′-ATGGCAACGACATGATCTCCG-3′
R: 5′-AGACCATGACCATGACGACGAG-3′ / 65 / 1.05 / 0.992 / 177
PeNIP1;4 / PH01001725G0380 / - / F: 5′-AGCATGGAGTTAATGGCCTGC-3′
R: 5′-GCAACTTCTGCACGAATTGGAC-3′ / 63 / 1.04 / 0.999 / 147
PeNIP2;1 / PH01000135G1100 / - / F: 5′-GATATCCATGACCTCTCCACGGT-3′
R: 5′-CGTCACGAACACCAGCAAGAAC-3′ / 64 / 1.04 / 0.995 / 150
PeNIP2;2 / PH01000065G1650 / - / F: 5′-TCCAACACCTCGAGGACCAAC-3′
R: 5′-CCACCTCCGATATGACCTTCTTG-3′ / 65 / 1.01 / 0.998 / 166
PeNIP3;1 / PH01001509G0200 / - / F: 5′-TCTGTAAATGAGGGCATGCAGG-3′
R: 5′-TGAGCTTCACCACCGTGTACATG-3′ / 64 / 1.04 / 1.000 / 167
PeNIP3;2 / PH01000508G0290 / - / F: 5′-TAGCCGCATCTGCAGGTCTAGT-3′
R: 5′-AGAATTTGTGCGGCCATGTAAG-3′ / 64 / 1.05 / 0.999 / 154
PeSIP1;1 / PH01000897G0530 / FP093349.1 / F: 5′-AAGGTGATGGCCATGGGAGC-3′
R: 5′-GACGAGGAGGGCGTAATGGA-3′ / 66 / 1.02 / 0.990 / 156
PeSIP2;1 / PH01000430G0050 / - / F: 5′-GTCTACCTCTTCACCGCCTTCG-3′
R: 5′-TGAAAGTTGCCAATCCTTCAGC-3′ / 64 / 0.96 / 0.999 / 160
NTB / - / FP091427.1 / F: 5′-TCTTGTTTGACACCGAAGAGGAG-3′
R: 5′-AATAGCTGTCCCTGGAGGAGTTT-3′ / 60 / 0.96 / 0.991 / 133
TIP41 / - / FP092936.1 / F: 5′-AAAATCATTGTAGGCCATTGTCG-3′
R: 5′-ACTAAATTAAGCCAGCGGGAGTG-3′ / 62 / 1.01 / 0.998 / 102

SupplementaryFig. 1Tissue specific expression analysis of PeAQPs in Ph.edulisusing qRT-PCR

1: Root;2: Stem;3: Leaf;4: Sheath.

Theexpression values in various tissues were normalized to that of in root. TIP41 was used asthe reference gene.Error bars represent standard deviation of biological triplicate with three technical replicates.

Supplementary Fig. 2qRT-PCR analysis of PeAQPs in root of Ph.edulis under stresses

1: Expression profiles inuntreated root; 2, 3 and 4:Expression profilesin root after stress treatments of drought, water and400 mmol·L-1NaClfor 2 h, respectively.

Thegene expression values were normalized to that ofuntreated root. TIP41 was used asthe reference gene.Error bars represent standard deviation of biological triplicate with three technical replicates.

Supplementary Fig. 3qRT-PCR analysis of PeAQPs in leaf of Ph.edulis under stresses

1:Expression profiles inuntreated leaf; 2, 3 and 4:Expression profilesin leaf after stress treatments of drought, water and400 mmol·L-1NaClfor 2 h, respectively.

Thegene expression values were normalized to that ofuntreated leaf. TIP41 was used asthe reference gene.Error bars represent standard deviation of biological triplicate with three technical replicates.