Figure SA1: Chemical structure of representative carboxamide fungicidesa
a Structural features:▲ carbonyl group interacting with a tyrosine residue in SdhD through hydrogen bonding (position 144 inB. cinerea); Δ oxygen or nitrogen atoms from heterocycles interacting, probably by hydrogen bonding, with a SdhB histidine (position 272 inB. cinerea); phenyl group interacting, probably through hydrophobic contacts, with a proline and two tryptophane residues from SdhB (positions 225, 228 and 229 inB. cinerea) (Figure 3) (1, 2, 3)
b molecules not tested in this study
1.Glättli, A., G. Stammler, T. Grote, and S. Schlehuber.2010. SDH Inhibitors: history, biological performance and molecular mode of action, p. 50-54. In G. U. Dehne DW, Kuck KH, Russell PE and Lyr H. (ed.), 16th international Reinhardsbrunn symposium, Friedrichroda, Germany.
2.Horsefield, R., V. Yankovskaya, G. Sexton, W. Whittingham, K. Shiomi, S. Omura, B. Byrne, G. Cecchini, and S. Iwata. 2006. Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase) - A mechanism of electron transfer and proton conduction during ubiquinone reduction. Journal of Biological Chemistry 281:7309-7316.
3.Huang, L. S., G. Sun, D. Cobessi, A. C. Wang, J. T. Shen, E. Y. Tung, V. E. Anderson, and E. A. Berry. 2006. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by Complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry 281:5965-5972.
Figure SA2: Tree of Botrytis individual strains generated by neighbor-joining analysis based on Da distance (1), showing the relationship between individual microsatellites and the presence or absence of the 143-144 intron in cytb.
Nodes with a bootstrap value (1000 repetitions) greater than 500 are indicated with a black circle.
Botrytis groupI isolates are shownby dotted boxes, whereas Botrytis group IIstrains are not dotted.
Isolates with the 143-144 intron are shown in dark gray, whereas the isolates in light gray do not have this intron.
Black arrows indicate strobilurin-resistant strains.
1.Nei, M., F. Tajima, and Y. Tateno.1983. Accuracy of estimated phylogenetic trees from molecular data. II Gene frequency data. Journal of Molecular Evolution 19:153-170.
Table SA1: Characteristic of the Champagne strains used for population analysis
Isolate / Location / Collection date / Host plant / Speciesa / Strb / Intronc / Bc1 / Bc2 / Bc3 / Bc4 / Bc5 / Bc6 / Bc7 / Bc10D340
D342
D359
D367
D368
D369
D373
D374
D377
D380
D383
C200
C201
C202
C203
C204
C205
C206
C207
C209
C210
C211
C212
C213
C214
C217
C218
C219
C221
C222
C223
C224
C228
C229
C230
C231
C232
C233
C234
C235
C236
C237
C243
C244
C246
C247
C248
C251
C252
C253
C255
C257
C258
C259
C270
C271
C272
C274
C299
C300
C216
C240
C249
C250
C254
D203
D210
D212
D215
D225
D226
D227
D228
D235
D236
D238
D241
D261
D265
D270
D279
D331
D230
D231
D233
D248
D256
D257
D296
D312
D320
C141
C143
C145
C146
C147
C148
C154
C161
C162
C163
C165
C166
C171
C173
C176
C144
C157
D142
D145
D147
D149
D161
D163
D164
D170
D171
D173
D177
D179
D180
D181
D182
D190
D193
D196
D197
D201R
D204R
D207R
D209
D165
D176
D184
D195
D200R
C109
C110
C111
C112
C114
C116
C117
C118
C120
C121
C122
C123
D105
D107
D109
D127
D136
D140
D22
D49
D72
D81
D84
D92
D94
D98
D99
D114
D117
D124
D21
D37
D7 / Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Courteron
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières
Vandières / June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
September 2005
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006
June 2006 / Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Brambles
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine
Grapevine / II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
I
I
I
I
I
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
I
I
I
I
I
I
I
I
I
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
I
I
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
I
I
I
I
I
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
I
I
I
I
I
I / R
R
S
S
S
S
R
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
R
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
R
S
S
S
S
S
S
R
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
R
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
R
S
S
S
S
S
S
S
S
S
S
S
R
S
R
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S / -
-
+
-
-
-
-
-
+
-
-
-
-
-
-
-
+
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
+
+
+
+
-
-
-
+
-
+
+
-
-
-
-
-
-
+
-
-
-
-
+
+
+
+
+
+
+
+
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
+
-
-
-
-
-
+
-
-
+
+
-
-
-
-
+
-
-
-
-
+
-
-
-
+
+
+
+
+
-
-
-
-
-
-
-
-
-
-
-
-
+
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
+
+
+
+
+ / 215
225
245
209
219
227
219
231
235
217
219
231
239
217
217
217
217
217
217
231
231
235
231
217
217
235
219
231
231
231
225
217
215
217
231
239
231
217
231
231
217
217
217
217
217
217
217
231
231
217
231
231
217
233
231
231
231
239
231
231
221
221
221
221
225
229
233
217
217
217
211
241
229
227
217
217
217
241
231
231
217
243
241
235
221
219
227
227
227
221
221
239
239
241
217
243
217
231
235
217
217
231
239
217
231
217
219
221
217
231
229
217
247
233
217
229
229
217
217
217
229
217
215
231
229
217
217
221
217
239
241
225
221
221
221
227
217
217
217
217
231
217
239
217
217
217
239
217
231
251
217
231
217
217
217
217
217
217
231
217
239
217
217
221
201
227
221
225
221 / 161
185
153
163
171
139
181
167
181
167
171
171
167
167
171
167
169
167
167
191
167
161
171
171
171
167
169
167
181
167
167
167
167
167
167
167
167
171
181
161
155
183
167
167
171
171
181
167
181
167
161
167
167
171
167
171
181
141
167
141
161
167
181
167
167
139
167
171
167
167
153
155
167
171
167
181
167
181
181
167
181
167
157
161
167
167
157
167
181
181
167
171
181
171
167
169
167
171
171
167
181
181
167
167
157
167
167
171
167
167
167
167
157
157
167
167
139
167
181
181
147
171
215
167
139
181
167
167
155
155
167
167
167
139
181
167
171
167
171
171
169
171
167
167
167
181
169
169
167
171
181
171
167
167
167
171
183
183
181
181
181
167
171
181
181
167
167
167
169 / 227
215
219
219
219
219
219
219
219
217
219
219
217
221
217
219
219
219
219
219
219
223
219
221
219
217
217
219
219
219
213
219
215
219
223
213
219
223
219
233
219
219
219
219
219
219
219
219
219
219
217
219
221
217
219
219
219
219
219
217
199
199
199
199
199
215
219
219
219
219
221
219
219
213
219
213
221
213
219
217
219
219
199
199
199
199
199
199
199
199
199
213
221
213
219
219
219
219
221
219
219
217
213
221
217
221
199
199
219
219
217
219
219
219
219
213
213
219
219
219
213
219
219
219
219
219
221
219
219
211
213
199
199
199
199
199
221
219
219
219
217
219
217
219
219
219
217
219
213
213
221
217
219
219
221
219
219
219
219
219
213
219
221
199
199
199
199
199
199 / 125
125
125
125
128
116
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
116
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
125
116
125
125
0
0
0
0
0
116
125
125
125
125
125
125
128
125
125
125
125
125
125
125
125
125
0
0
0
0
0
0
0
0
0
125
125
125
125
125
125
125
125
125
125
116
125
125
116
125
0
0
125
125
125
125
125
125
125
116
116
125
125
125
116
125
125
125
116
125
125
125
125
125
125
0
0
0
0
0
125
125
125
125
116
125
125
125
125
116
125
125
125
125
125
125
125
125
125
125
125
125
125
116
125
125
125
0
0
0
0
0
0 / 157
157
157
157
165
157
147
161
161
159
165
159
159
157
147
147
157
147
147
147
147
163
157
147
159
159
147
147
159
159
153
147
161
163
159
153
159
165
159
157
145
157
147
157
157
163
165
147
159
147
163
159
258
163
147
165
159
165
147
147
147
147
165
147
147
155
151
147
147
147
151
157
147
159
165
159
163
153
159
147
151
147
157
159
147
157
151
147
151
151
147
153
157
165
163
151
163
163
163
147
165
161
153
163
161
163
165
157
157
147
159
163
155
151
159
155
155
147
163
165
155
165
157
147
155
147
163
159
163
153
153
157
147
115
147
147
157
157
165
165
159
165
159
165
157
159
147
163
147
153
147
159
147
157
163
163
157
147
159
165
153
163
157
147
165
147
147
163
153 / 134
126
124
118
120
124
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
130
120
120
120
120
130
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
120
140
120
120
120
120
120
120
86
86
86
86
86
128
124
120
120
120
124
120
120
130
120
120
120
130
120
120
120
120
86
86
86
86
86
86
86
86
86
130
120
130
120
120
120
120
120
120
120
124
130
120
124
120
86
86
120
120
120
120
120
124
120
128
128
120
120
120
128
120
124
120
128
120
120
120
120
132
130
86
86
86
86
86
120
120
120
120
120
120
120
120
120
120
120
120
120
130
120
120
120
120
120
120
120
120
120
120
130
120
120
86
86
86
86
86
86 / 131
125
119
129
119
113
121
113
113
113
119
119
119
121
119
119
117
117
117
119
117
119
119
119
119
113
113
119
119
113
117
117
119
119
121
123
119
121
119
121
117
119
119
121
117
119
119
119
117
119
113
119
121
113
119
113
119
119
119
119
113
115
119
117
119
113
125
117
119
119
117
117
119
117
119
117
117
123
119
117
119
115
129
113
115
115
115
115
115
115
115
123
121
119
121
113
119
117
117
117
119
117
123
119
119
121
115
115
121
121
117
117
121
117
119
127
123
117
119
121
113
119
127
121
113
119
121
117
117
123
123
115
113
155
115
113
119
119
119
121
113
119
121
119
119
117
117
119
117
121
119
113
119
119
121
119
119
119
113
121
123
119
119
115
115
113
115
113
115 / 175
147
147
175
173
175
181
185
181
183
183
181
183
167
181
181
181
189
183
181
185
183
183
183
167
181
183
185
181
181
167
185
181
181
181
167
185
181
181
183
181
181
185
167
181
181
183
185
181
181
181
185
167
183
185
181
181
181
189
173
159
159
159
159
159
175
185
181
181
181
167
183
183
167
185
183
181
167
185
185
185
181
161
159
159
161
159
159
159
159
159
167
185
181
183
173
181
167
183
181
181
173
167
183
173
181
159
159
167
185
185
181
183
185
189
173
173
185
189
181
173
183
173
185
173
183
183
185
181
169
167
159
159
159
159
159
167
167
181
181
181
181
181
181
181
181
181
167
181
167
189
183
181
167
167
181
181
183
181
181
167
181
167
159
159
159
179
159
159
aBotrytis species: II is Botrytis group IIand I is Botrytis group I.
bcytb genotype, in regard to the G143A change. Determined by digestion of the Qo13ext/Qo14ext PCR fragment with the restriction enzyme SatI. Strains with a double-band pattern were considered R (resistant to strobilurins).
c Absence (-) or presence (+) of the 143-144 1205 bp intron, determined based on the length of the Qo13ext/Qo14ext PCR fragment
Table SA2: Primers used to sequence cytb, sdhA, sdhB, sdhC and sdhD in Botrytis group I and group II.
Primer / Sequence (5’-3’) / OrientationCytochrome b
Qo1
Qo2
Qo3
Qo4
Qo5
Qo6
Qo7
Qo8
Qo9
Qo10
Qo11
Qo12
Qo13
Qo14
Qo15
Qo16
Qo17
Qo18
Qo19
Qo20
Qo21
Qo22
Qo13ext
Qo14ext / ACCGAATGGTGGGATCAATA
TCCGTAGGTTTCCTGCTGAT
TTTAGCAATGCACTACAACCCTA
AAAAATCTTCGACAATAAAAGAACA
ATTTAAAATAAGTTTGCATATTGACGA
CATAAACCTTCAGTAATAAAGCCATTC
GCAATAGCTAACACAGATGCTTT
TCACGCATAATCAAACTCTGC
GAGCGTCTAGGGATTAGACCA
TTCCACTTAAACCTCTTGTGTCTTT
GGGGATATTGTACAAGTTGTGAATTC
ACCTTTTATATGGCCGACGA
ACCCGACGGGGTTATAGAAT
CCATCTCCATCCACCATACC
GAGTGCTGTACCATGAATTGGA
ACCTTCAGCCATTGTGAACC
GGAAAGATCTGCCTTCTGTTTT
TCGTTGAACGTTCTTGATTCC
TCATTTGCAGCGAAGTTTGA
AGCACTTAACATAGCAATAACACCT
AATGCAAACACCACCAGCTA
TCCGAGATACCAGTAGCGTTTA
GGTATAACCCGACGGGGTTATAGAATAG
AACCATCTCCATCCACCATACCTACAAA / F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
Succinate dehydrogenase subunit A
SdhA1
SdhA2
SdhA3
SdhA4
SdhA5
SdhA6
SdhA7
SdhA8 / ATCGAGCCATCATCGCTATT
TCTCCCAACCAATCTGAACC
AAGAGCAGCATTCGGTCTTG
ACCCTGGGAGTTGAGGAGAT
TGGTTACGGACGTGCCTACT
TGCATGGCTAGACGAACATC
GCGATAACTTCGAACCAGGA
CTCCAATTCCCAACCAGCTA / F
R
F
R
F
R
F
R
Succinate dehydrogenase subunit B
IpBcBeg
IpBcEnd2 / CCACTCCTCCATAATGGCTGCTCTCCGC
CTCATCAAGCCCCCTCATTGATATC / F
R
Succinate dehydrogenase subunit C
SdhC1
SdhC2
SdhC3
SdhC4
SdhC5 / ATCACGTGCCAGATTTCCTT
TTACAAGCCCTCCAAATTGC
CCAAATAAAGGCGAAGCAAG
TGCCTCCCAAGACAGATACC
AGGTGGGATCTTGGGGTTAT / F
R
R
F
R
Succinate dehydrogenase subunit D
SdhD1
SdhD2 / TGATGACCTTGCACGTGACT
AGCAGCATGTGTTTGACAGC / F
R
Table SA3: In vitro effects of fungicides on germ-tube elongation inB. cinerea strains (EC50 in µM)
Strain number / QoIs / QiIs / Carboxamides / SDHI inh. / Decoupler / Multisite inh.Azoxystrobin / Pyraclostrobin / Antimycin A / Boscalid / Flutolanil / Carboxin / TTFA / Fluazinam / Tolylfluanid
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50 / 0.10
0.15
0.12
0.25
0.37
0.50
0.25
0.30
>25
>25
0.50
0.50
>25
>25
0.74
0.62
0.74
0.50
2.0
3.0
2.5
2.7
0.50
0.50
>25
>25
>25
>25
>25
>25
>25
>25
>25
>25
>25
>25
>10
>10
>10
>10
>10
>10
>10
>10
>10
>10
>25
>25
0.37
>10 / 0.015
0.010
0.010
0.018
0.013
0.015
0.013
0.013
>10
>10
0.13
0.077
>10
>10
0.052
0.077
0.052
0.052
0.15
0.23
0.21
0.26
0.021
0.015
>10
>10
>10
>10
>10
>10
>10
>10
>10
>10
>10
>10
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>2.5
>10
>10
0.015
>2.5 / 0.006
0.004
0.004
0.019
0.019
0.017
0.009
0.009
0.009
0.009
0.028
0.056
0.028
0.028
0.022
0.019
0.019
0.015
0.093
0.093
0.112
0.131
0.013
0.015
0.013
0.011
0.019
0.017
0.015
0.013
0.015
0.015
0.015
0.013
0.015
0.015
0.019
0.015
ND
0.015
ND
0.015
ND
0.019
0.019
0.015
0.017
0.015
0.019
0.017 / 0.029
0.029
0.044
0.15
0.087
0.073
0.087
0.12
0.087
0.087
0.087
0.12
0.15
0.10
0.73
0.73
0.44
0.73
1.75
1.17
0.87
1.46
5.0
5.8
5.8
5.0
4.4
3.5
2.9
2.9
2.9
2.6
4.4
2.9
2.9
2.9
6.4
4.4
5.8
4.4
4.4
5.8
5.2
6.4
5.8
9.3
>36
>36
>36
>36 / 24.8
27.8
31.0
31.0
27.8
23.2
27.8
18.6
31.0
37.2
26.3
37.2
37.2
31.0
31.0
27.8
37.2
27.9
27.9
34.0
34.0
37.2
3.7
5.3
4.6
4.3
6.2
4.6
5.6
4.6
4.6
4.6
24.8
21.7
31.0
15.5
21.7
23.3
37.2
31.0
23.3
21.7
27.8
21.7
37.2
46.4
52.6
40.2
43.3
37.2 / 1.3
1.5
1.1
2.1
1.7
2.1
2.6
1.7
2.1
2.6
2.1
2.6
2.6
2.6
3.8
3.0
3.4
3.8
5.1
6.0
4.7
5.1
15.7
16.2
17.0
17.8
14.9
13.6
14.9
12.8
19.1
10.6
15.7
17.0
12.8
14.9
10.6
12.8
10.6
10.6
17.2
17.2
8.5
12.8
5.1
38.3
>43
>43
8.5
5.1 / 63.1
76.6
90.0
76.6
76.6
67.6
76.6
45.0
72.1
49.5
45.0
54.1
90.0
90.0
54.1
90.0
72.1
54.1
76.6
76.6
76.6
67.6
76.6
90.0
67.6
72.1
67.6
72.1
58.6
76.6
54.1
76.6
76.6
67.6
58.6
81.1
76.6
67.6
58.6
67.6
90.0
58.6
90.0
90.0
90.0
90.0
67.6
54.1
72.1
58.6 / 0.011
0.009
0.009
0.032
0.032
0.022
0.022
0.022
0.022
0.032
0.043
0.065
0.054
0.075
0.022
0.032
0.022
0.032
0.086
0.075
0.086
0.108
0.022
0.022
0.022
0.032
0.032
0.022
0.043
0.022
0.043
0.022
0.043
0.043
0.022
0.022
0.032
0.043
ND
0.026
ND
0.022
ND
0.043
0.043
0.032
0.026
0.022
0.032
0.032 / 0.058
0.086
0.058
0.43
0.23
0.26
0.35
0.29
0.43
0.72
0.29
0.43
0.23
0.35
0.72
0.58
0.43
0.35
0.86
0.72
0.72
0.58
0.23
0.29
0.58
0.58
0.72
0.35
0.43
0.58
0.58
0.29
0.58
0.35
0.29
0.26
0.43
0.43
ND
0.35
ND
0.58
ND
0.29
0.58
0.43
0.43
0.58
0.23
0.43