Fig.S1. Minimum-evolutionphylogenetic treebased on 16SrRNA gene sequences of the new isolates YIM93656T, YIM 90917 and closely related type strains in the family Halobacteriaceae.MethanosarcinalacustrisDSM 13486T(AF432127) was usedas an outgroup.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampleddatasets.
Fig.S2. Maximum-parsimony phylogenetic treebased on 16SrRNA gene sequences of the new isolates YIM93656T, YIM 90917 and closely related type strains in the family Halobacteriaceae.MethanosarcinalacustrisDSM 13486T(AF432127) was usedas an outgroup.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampleddatasets.
Fig.S3. Maximum-likelihood phylogenetic treebased on 16SrRNA gene sequences of the new isolates YIM93656T, YIM 90917 and closely related type strains in the family Halobacteriaceae.MethanosarcinalacustrisDSM 13486T(AF432127) was usedas an outgroup.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampled datasets.
Fig.S4. Minimum-evolutionphylogenetic tree,based on rpoB′sequences of the new isolates YIM93656T, YIM 90917 and closely related type strains in the family Halobacteriaceae.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampleddatasets.
Fig.S5. Maximum-parsimony phylogenetic treebased on rpoB′sequences of the new isolates YIM93656T, YIM 90917 and closely related type strains in the family Halobacteriaceae.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampleddatasets.
Fig.S6. Maximum-likelihood phylogenetic treebased on rpoB′sequences of the new isolates YIM93656T,YIM 90917 and closely related type strains in the family Halobacteriaceae.Numbers at the nodes indicate the bootstrap values(﹥70%) based on 1000 resampled datasets.
Fig.S7 Thin-layer chromatogram of polar lipids extracted of the two strains YIM93656T, YIM 90917and related members
The chromatographic conditions were as follows: Silica Gel 60 thin-layer plates (10×10cm) were spotted with 10.0 μl of a whole-cell lipid extract. Chloroform/methanol/water (65:25:4, by vol) was used in the first direction, and chloroform/acetic acid/methanol/water (80:18:12:5, by vol) was used in the second direction. Plates (a-e) were sprayed withphosphomolybdate reagent, followed by heating at 150 oC for 3 min to detect all polar lipids. Plates (f-k) were sprayed with sulfuric acid/ethonal (1:2,by vol) followed by heating at 150 oC for 3 min to detect phospholipids and glycolipids by colors. Plate (k) was a single TLC with the exhibition layer agentchloroform/acetic acid/methanol/water (80:25:12:5, by vol). Notes: a, f, 4 and 9, YIM 93656T; b, g, 3 and 10, YIM 90917; c, h, 1 and 7, Haladaptatus paucihalophilusJCM13897T; d, i and 5, Haladaptatus litoreusJCM15771T; e, j and 2, Haladaptatus cibariusJCM15962T;6 and 10,HalopelagiusinordinatusRO5-2T; PG, phosphatidylglycerol; PGP-Me, phosphatidylglycerolphosphate methyl ester; PGS , phosphatidylglycerol sulfate; S-DGD-1, sulfated mannosyl glucosyl diether; GL, glycolipid; F, first dimension of TLC; S, second dimension of TLC.
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