Expression of borage Δ6 desaturase in Saccharomyces cerevisiae and oilseed crops
ArticleinCanadian Journal of Botany 80(1)·February 2011
Impact Factor: 1.40 · DOI: 10.1139/b01-130
Xiao Qiu
30.38 · University of Saskatchewan
Haiping Hong
12.78 · BASF Corporation
12.78
5 publications 287 citations
Haiping Hong
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BASF Corporation
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GB Development
Nagamani Datla
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Samuel L MacKenzie
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David Taylor
32.83 · National Research Council Canada
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National Research Council Canada
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Terry L Thomas
37.70 · Texas A&M University
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Terry L Thomas
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Abstract
The borage Δ6 desaturase is a modular protein that contains a cytochrome b5-like domain attached to the N-terminus of the main catalytic domain. When the full-length open reading frame was expressed in two types of Saccharomyces cerevisiae strains, the endogenous cytochrome b5-disrupted strain and the cytochrome b5-competent wild type strain, the expressed enzyme introduced a double bond at the Δ6 position into the endogenous substrates 16:1(9) and 18:1(9), and also into the exogenously fed fatty acid substrates 18:2(9,12) and 18:3(9,12,15). However, the preferred substrates of the enzyme when expressed in yeast were 18:2(9,12) and 18:3(9,12,15), whereas 16:1(9) and 18:1(9) were relatively poor substrates. When the partial desaturase sequence with a deletion of the cytochrome b5-like domain at the N-terminus was introduced into the yeast strains, the truncated enzyme could not desaturate any of the supplied substrates. Introduction of the full-length cDNA into flax (Linum usitatissimum L.) under the control of a constitutive (35S) promoter resulted in accumulation of the two Δ6 desaturated fatty acids (18:3(6,9,12) and 18:4(6,9,12,15)), in one elite line, forming up to 22% of the total fatty acids in the stem, 19% in the root, and 11% in the leaf. Introduction of the desaturase into Brassica juncea (L.) Czern. under the control of the Brassica napus L. napin promoter resulted in accumulation of the Δ6 desaturated fatty acids at levels of up to 13% of the total fatty acids in mature seeds.Key words: borage, Δ6 desaturase, γ-linolenic acid, stearidonic acid, Brassica juncea, flax.
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Haiping Hong
12.78 · BASF Corporation
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