EST cDNA Array

The table below shows the arrangement of spots on the macroarrays. Each spot (except the controls - actin, GAPDH, EIF and neg) are give a number (for example, e.g. Gmm-1694 is 1, Gmm-0596 is 3). These numbers are used to identify each spot on the Excel spreadsheets on which the data areis presented (lehanemacroarraydata.xls). Background and negative control values have already been subtracted from each experimental value. Any negative values resulting were set to zero. For normalization of total intensity on each array the mean of the internal control spots (GAPDH, actin, EIF) was averaged for each membrane separately. Values for the other spots on the membrane were then expressed relative to this average for the internal control spots. These normalised figures are presented on the Excel spreadsheet, (lehanemacroarray.xls). The values are also presented in the order in which they appear in Tables 1-3 of the paper. Analysis of thisese data was then performed as described in the paperMaterials and methods in the paper.

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A / C1-26
Gmm-2387*
Actin / 1
Gmm-1694
Iron met / 2
Gmm-2445
Chitin binding / 3
Gmm-0596
Toll pathway / 4
Gmm-2522
MAP
kinase / 5
Tse15b02
Toll pathway / 6
Gmm-1963
Attacin / 7
Gmm-3328
Scavenger
receptor / 8
Gmm-0072
Scavenger receptor / 9
Tse29b09
Scavenger
receptor / 10
Gmm-3236
Adhesion/
lectin / C2-27 Gmm-2682*
GAPDH
B / 11
Gmm-0048
Chitin
binding / 12
Gmm-2709
Chitin
binding / 13
Tse33h03
Adhesion/
lectin / 14
Tse36b05
Opsonization / 15
Gmm-2904
Chitin
binding / 16
Tse37e05
Toll pathway / 17
Gmm-2058
Extracellularperoxidase / 18
Gmm-1270
Imd pathway / 19
Gmm-2594
Serpin / 20
Tse41c10
JNK cascade / 21
Gmm-3352
Serpin / 22
Tse44a11
Toll pathway
C / 23
Tse48e12
2 macro-
globulin / 24
Gmm-2945
Chitin
binding / 25
Tse51g11
Toll path-
way / 26 Tse59b02
scavenger
receptor / 27
Gmm-0398
Chitin
binding / 28
Gmm-2608
Scavenger
receptor / 29
Tse66g09
JAK_STAT
Cascade / 30
Tse70a12
Binding
bacteria / 31
Gmm-2766
Serpin / 32
Gmm-3334
Serpin / 33
Tse72e04
Serpin / 34
Tse72e08
Serpin
D / 35
Gmm-1329
Chitin
binding / 36
Tse74h11
Chitin
binding / 37
Gmm-2412
Adhesion/
lectin / 38
Tse81d11
Binding
bacteria / 39
Gmm-3029
Translation regulation / 40
Gmm-2356
Serpin / 41
Tse84d03
Scavenger
receptor / 42
Gmm-1598
Serine car-
boxypep / 43
Tse91h03
Serine car
boxypep / 44
Tse94c04
Serpin / 45
Gmm-0034
Iron
metabolism / 46
Gmm-2386
Chitin
binding
E / 47
Tse98g05
Chitin
binding / 48
Gmm-2629
Oxidative
stress / 49
Tse100h09
Iron metab
olism / 50
Tse105c10
2 macro-
globulin / 51
Gmm-3262
Chitin binding / 52
Tse108b10
Scavenger
receptor / 53
Tse112d02
Serine endo-
peptidase / 54
Gmm-2087
Peroxidase / 55
Gmm-0033
Serpin / 56
Gmm-3348
Iron metab
olism / 57
Gmm-1799
Oxidative
stress / 58
Tse121d04
Chitin
binding
F / neg / 59
Gmm-3099
Peroxidase / 60
Gmm-2619
Peroxidase / 61
Gmm-0601
Extracellular peroxid / 62
Gmm-3093
Chitin
binding / 63
Tse126a11
Extracellular peroxid / 64
Gmm-3156
Binding
bacteria / 65
Tse129g02PPO
activation / 66
Gmm-3307
Iron met
abolism / 67
Gmm-2843
Chitin
binding / 68
Tse131b02
MAP
kinase / C3-40
C1088*
EIF