Electronic Supplementary Materials s6

Electronic supplementary materials

Table S1: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Clermont-Ferrand in 2000. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-.

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 670.4/567.3 / 0.77 / 362.1/299.6 / 757.0/693.4 / 681.7/600.3 / 713.0/587.6 / 0.89/0.67
GPA / 16807/14103 / 21.3 / 8447/7381 / 21592/18310 / 16194/13944 / 18614/15136 / 0.90/0.67
TKW / 40.31/40.44 / 28.56/32.79 / 53.37/53.50 / 42.28/43.11 / 38.35/38.89 / 0.95/0.88
ADM / -/- / -/- / -/- / -/- / -/- / -/-
NTOT / -/- / -/- / -/- / -/- / -/- / -/-
NS% / -/- / -/- / -/- / -/- / -/- / -/-
NSA / -/- / -/- / -/- / -/- / -/- / -/-
GPC / 2.21/1.92 / 0.016 / 1.93/1.49 / 2.72/2.35 / 2.17/1.94 / 2.14/1.92 / 0.86/0.74
GPY / 14.75/10.91 / 0.125 / 9.38/6.27 / 18.00/14.54 / 14.81/11.67 / 15.25/11.35 / 0.78/0.49
NHI / -/- / -/- / -/- / -/- / -/- / -/-

Table S2: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Clermont-Ferrand in 2001. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-..

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 661.2/592.0 / 1.19 / 285.2/279.4 / 856.3/772.6 / 792.5/732.4 / 781.3/533.7 / 0.91/0.87
GPA / 19941/17796 / 44.7 / 7883/7207 / 27159/27018 / 22458/21616 / 17977/16321 / 0.87/0.88
TKW / 33.37/33.83 / 0.047 / 26.15/23.61 / 44.76/44.71 / 35.34/33.90 / 32.34/32.66 / 0.87/0.94
ADM / 1532/1358 / 3.08 / 807/704 / 2101/2097 / 1721/1650 / 1257/1165 / 0.79/0.84
NTOT / 19.28/14.55 / 0.025 / 12.62/7.50 / 25.42/19.78 / 20.69/16.17 / 16.41/12.64 / 0.72/0.58
NS% / 0.74/0.60 / 0.014 / 0.54/0.33 / 1.08/0.91 / 0.67/0.58 / 0.81/0.63 / 0.44/0.43
NSA / 6.46/4.57 / 0.001 / 3.16/1.91 / 11.11/10.22 / 6.18/5.28 / 5.45/4.02 / 0.51/0.58
GPC / 1.95/1.70 / 0.015 / 1.64/1.24 / 2.83/2.23 / 1.83/1.49 / 1.88/1.61 / 0.88/0.65
GPY / 12.81/10.00 / 0.114 / 7.33/5.60 / 16.29/13.96 / 14.51/10.90 / 12.61/7.82 / 0.82/0.62
NHI / 0.67/0.69 / 0.0006 / 0.79/0.46 / 0.87/0.78 / 0.70/0.67 / 0.67/0.68 / 0.58/0.67

Table S3: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Mons in 2001. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-.

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 754.3/468.2 / 0.84 / 480.0/277.9 / 897.7/601.7 / 870.1/550.8 / 733.6/408.2 / 0.87/0.69
GPA / 18804/11853 / 23.7 / 12182/6824 / 23578/16733 / 20369/13587 / 17953/10007 / 0.90/0.73
TKW / 40.39/39.70 / 0.047 / 30.77/30.87 / 52.21/49.87 / 42.81/40.52 / 40.90/40.90 / 0.97/0.96
ADM / 1407/925 / 1.50 / 876/551 / 1653/1326 / 1627/1079 / 1289/761 / 0.86/0.67
NTOT / 16.72/7.72 / 0.015 / 12.24/5.22 / 19.64/10.19 / 18.56/9.10 / 15.82/6.64 / 0.56/0.39
NS% / 0.47/0.32 / 0.007 / 0.28/0.21 / 0.64/0.47 / 0.46/0.31 / 0.48/0.31 / 0.42/0.20
NSA / 3.10/1.45 / 0.005 / 1.79/0.75 / 4.62/2.29 / 3.45/1.62 / 2.66/1.11 / 0.57/0.33
GPC / 10.32/7.64 / 0.009 / 8.27/6.21 / 13.05/10.43 / 9.92/7.70 / 10.20/7.75 / 0.79/0.70
GPY / 77.58/35.74 / 0.072 / 57.17/24.40 / 91.31/19.04 / 86.13/42.58 / 75.01/31.52 / 0.57/0.40
NHI / 0.81/0.81 / 0.0003 / 0.74/0.71 / 0.87/0.87 / 0.81/0.82 / 0.83/0.83 / 0.63/0.36

Table S4: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Le Moulon in 2000. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-.

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 794.9/501.9 / 0.96 / 415.9/154.7 / 976.5/699.8 / 868.8/541.1 / 839.6/500.3 / 0.87/0.78
GPA / 22521/13608 / 29.5 / 12154/4694 / 29847/19142 / 21890/13520 / 24000/13622 / 0.89/0.83
TKW / 35.60/37.14 / 0.048 / 24.00/27.95 / 46.24/46.46 / 39.71/40.03 / 35.02/36.74 / 0.96/0.97
ADM / 1593/1055 / 1.84 / 874/606 / 1921/1507 / 1733/1146 / 1598/1000 / 0.78/0.65
NTOT / 19.78/7.74 / 0.018 / 13.35/5.16 / 24.06/13.29 / 20.22/7.92 / 19.77/7.51 / 0.68/0.71
NS% / 0.61/0.23 / 0.001 / 0.31/0.14 / 1.11/0.47 / 0.56/0.24 / 0.60/0.23 / 0.72/0.71
NSA / 4.79/1.36 / 0.010 / 2.78/0.49 / 9.20/2.80 / 4.83/1.52 / 4.26/1.17 / 0.69/0.66
GPC / 10.77/7.30 / 0.012 / 9.29/5.99 / 12.83/10.20 / 10.09/10.15 / 10.49/7.24 / 0.87/0.90
GPY / 85.50/36.37 / 0.117 / 52.21/15.79 / 109.33/59.79 / 87.72/36.48 / 88.41/36.14 / 0.82/0.73
NHI / 0.76/0.82 / 0.0005 / 0.58/0.54 / 0.86/0.93 / 0.76/0.81 / 0.78/0.84 / 0.80/0.74

Table S5: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Le Moulon in 2001. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-..

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 957.7/853.0 / 1.22 / 608.9/526.1 / 1181.8/1111.7 / 1097.4/989.2 / 978.6/867.5 / 0.85/0.84
GPA / 25908/22450 / 43.7 / 13168/13059 / 37492/3114 / 29230/25560 / 25671/22324 / 0.91/0.90
TKW / 37.39/38.29 / 0.051 / 24.90/28.56 / 53.02/51.35 / 37.60/38.74 / 30.08/38.88 / 0.97/0.97
ADM / 1812/1591 / 2.24 / 1170/881 / 2190/1986 / 2176/1919 / 1712/1519 / 0.80/0.83
NTOT / 22.98/19.97 / 0.024 / 15.35/12.08 / 27.95/20.88 / 25.68/18.34 / 22.74/17.17 / 0.70/0.62
NS% / 0.55/0.40 / 0.001 / 0.33/0.24 / 0.83/0.60 / 0.49/0.37 / 0.52/0.41 / 0.80/0.61
NSA / 4.69/2.94 / 0.010 / 2.25/1.85 / 8.41/4.37 / 5.26/3.51 / 3.78/2.67 / 0.80/0.51
GPC / 10.94/9.41 / 0.011 / 9.01/8.09 / 13.97/11.51 / 10.60/8.49 / 11.06/9.52 / 0.85/0.81
GPY / 104.25/79.91 / 0.158 / 64.58/53.75 / 126.54/98.33 / 116.39/84.53 / 108.07/82.65 / 0.71/0.66
NHI / 0.80/0.83 / 0.0004 / 0.66/0.75 / 0.88/0.88 / 0.80/0.81 / 0.83/0.84 / 0.85/0.61

Table S6: Mean, Standard Error of the Difference between two means (SED), minimal and maximal values as well as heritabilities observed for the DH line population in Chartainvilliers in 2000. To give a notion of transgression, mean value observed for the two parents Arche and Récital are also reported. Bold values correspond to N+ and regular values to N-.

Traits / Mean / SED / Minimal value / Maximal value / Arche mean value / Récital mean value / Heritabilities
GY / 652.4/491.5 / 0.78 / 414.4/288.4 / 780.1/615.6 / 744.0/538.4 / 677.7/505.1 / 0.84/0.41
GPA / 19635/13914 / 24.1 / 11583/8264 / 24726/18565 / 21420/14170 / 20721/14095 / 0.82/0.58
TKW / 33.54/33.48 / 0.046 / 21.19/26.40 / 44.71/45.97 / 34.88/37.95 / 32.79/35.90 / 0.86/0.89
ADM / 1369/991 / 1.43 / 790/537 / 1690/1246 / 1555/1062 / 1351/998 / 0.66/0.23
NTOT / 17.40/9.59 / 0.019 / 11.11/6.55 / 22.04/12.45 / 17.58/9.89 / 17.40/10.01 / 0.56/0.00
NS% / 0.72/0.48 / 0.001 / 0.45/0.31 / 1.00/0.72 / 0.60/0.46 / 0.71/0.44 / 0.55/0.32
NSA / 5.14/2.42 / 0.009 / 2.81/1.30 / 7.87/3.88 / 4.82/2.44 / 4.79/2.19 / 0.33/0.04
GPC / 10.66/8.32 / 0.015 / 8.55/10.32 / 13.11/10.49 / 9.80/7.87 / 10.60/8.84 / 0.65/0.72
GPY / 69.71/40.87 / 0.126 / 44.97/28.67 / 93.37/52.44 / 72.73/42.47 / 71.88/44.57 / 0.77/0.22
NHI / 0.70/0.75 / 0.0005 / 0.54/0.63 / 0.81/0.83 / 0.73/0.75 / 0.72/0.78 / 0.60/0.41

Table S7: Genomic regions involved in the control of Grain Protein Content (GPC), except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.

Genomic Region / N+ / N- / Interaction / Factorial regression
1A2 / Moul00
1B-1 / Moul01
2A1 / Mons01
Mons00 / Ordinate NNI=0.5
2A2 / Cler01, Moul01 / Nick00
Cler01
Moul01 / Ordinate NNI=0.5
3B-2 / Moul01
3D1 / Mons01
3D2 / Moul01
4B-4 / Moul01 / Moul01
5A1-1 / slope
5A2-2 / Cler01
5B-1 / Mons00
Cler01 / Ordinate NNI=0.5
7D3 / Cler00

Table S8: Genomic regions involved in the control of Straw N content (NS%), except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.

Genomic region / N+ / N- / Interaction / Factorial regression
2A1 / Moul00
Cler01
2A2 / Mons00 / Mons00
2B1 / Moul01
2B2 / Moul01
5A1-3 / Cler01
Mons00 / Mons00
5A2-1 / Mons01
5D-2 / Moul00 / Moul00
7A1 / Moul01
7B1 / Moul01
7B2-1 / Nick00

Table S9: Genomic regions involved in the control of Grain Yield (GY) except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.

Genomic Region / N+ / N- / Interaction / Factorial regression
2A2 / Cler01
3D-2 / Cler01 / Mons01
5A1-2 / Nick00
5A1-3 / Cler01
5A2-1 / Nick00
Cler01
Nick00 / R²
5B-1 / Mons01

Table S10: Genomic regions involved in the control of Grain number per Area (GPA) except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.

Genomic region / N+ / N- / Interaction / Factorial regression
3A / Cler01
3B-1 / Cler01 / R²
3D-1 / Mons01
Moul00 / Moul01
3D-2 / Cler01 / Cler01
5A1-1 / slope
5B-1 / Mons01
7B2-3 / slope

In the table are reported the genomic regions, as well as the location and year where QTL were detected (for N+, N- and interaction column), or the type of factorial regression variable that was considered (factorial regression column). Moul00 = Moulon in 2000, Mons00 =Mons in 2000, Cler00= Clermont-Ferrand in 2000, Nick00= Nickerson site in 2000, Moul01 = Moulon in 2001, Mons01 = Mons in 2001, Cler01 = Clermont-Ferrand in 2001. ordinate NNI= 0.5 stand for the ordinate of the regression that corresponds to an NNI value of 0.5, slope stands for the regression slope and R² stands for the regression determination coefficient.
Table S11: Genomic regions involved in the control of Total N Amount (NTOT), except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.

Genomic Region / N+ / N- / Interaction / Factorial regression
1B-1 / Ordinate NNI=0.5
2A1 / Moul00
3B-1 / Nick00
3B-2 / Nick00
5A1-1 / Ordinate NNI=0.5
5A1-2 / Mons01

In the table are reported the genomic regions, as well as the location and year where QTL were detected (for N+, N- and interaction column), or the type of factorial regression variable that was considered (factorial regression column). Moul00 = Moulon in 2000, Mons00 =Mons in 2000, Cler00= Clermont-Ferrand in 2000, Nick00= Nickerson site in 2000, Moul01 = Moulon in 2001, Mons01 = Mons in 2001, Cler01 = Clermont-Ferrand in 2001. ordinate NNI= 0.5 stand for the ordinate of the regression that corresponds to an NNI value of 0.5, slope stands for the regression slope and R² stands for the regression determination coefficient.
Table S12: Genomic regions involved in the control of Straw N Amount (NSA), except regions including rht-B1(4B) and Ppd-D1 (2D1). N+ corresponds to the genomic regions detected under N+, N- corresponds to the ones detected under N-, Interaction corresponds to the ones detected for interaction variables, and factorial regression to the ones detected for factorial regression variables.