Electronic Supplementary Material

A molecularly imprinted polymer for solid phase extraction of allantoin

Xianwen Zhonga,b, Fang Denga,b, Yuehua Wanga,b*, Xubiao Luoa,b*

a Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, Nanchang 330063, PR China

b College of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, PR China

* Corresponding author. Tel.: +86 791 83953372; Fax: +86 791 8395373.

E-mail addresses: , .

HLPC analysis

Chromatographic analysis was conducted on a Shimadzu HPLC system (LC-20AD, Shimadzu, Japan, www.shimadzu.com), which is consisted of a HPLC pump operating at a flow rate of 0.8 mL•min-1, and SPD-20A monitoring with the effluent at 224 nm for allantoin and hydantoin and 190 nm for urea. The analytical column was a 250 mm × 4.6 mm, 5 um C18 column (GL sciences, Japan, www.glsciences.com). The mobile phase was methanol: water (1:39, v/v).

Preparation of MH-SiO2

MH-SiO2 was prepared according to the report by Zhang et al. [30]. Briefly, 5 mL TEOS and 5 mL KH-570 were added into 100 mL ultrapure water under vigorous stirring (400 rpm) for 2 h. Then 0.4 mL of concentrated ammonia was added dropwise into the mixture solution and the reaction proceeded at room temperature for 2 h under constant stirring. The obtained MH-SiO2 was separated by centrifugation and washed with methanol and ultrapure water to remove impurities, then was dried at 80 oC under vacuum for future use.


Preparation of loading sample

A contain quality of dry powder of Rhizoma Dioscoreae was added into the solvent (ultrapure water/ethanol, v/v, 5:1), and then was under ultrasonic condition at 40 oC for 0.5 h. The mixture was centrifuged and the supernatant was collected. The volume of the obtained mixture was measured, and put it into oven to volatilize solvent. Finally, the obtained substance was dissolved in DMF.

Fig. S1 Synthesis route of MH-SiO2@MIP

Fig. S2 Langmuir (A) and Freundlich (B) plots for allantoin adsorption on MH-SiO2@MIP and MH-SiO2@NIP


Fig. S3 Scatchard plots of MH-SiO2@MIP and MH-SiO2@NIP

Fig. S4 Linear plots of t/Q vs t for MH-SiO2@MIP and MH-SiO2@NIP

Fig. S5 Adsorption selectivity of MH-SiO2@MIP and MH-SiO2@NIP towards alantoin, urea and hydantoin

Fig. S6 Regeneration of MH-SiO2@MIP

Table S1 Langmuir and Freundlich adsorption isotherm constants for allantoin on MH-SiO2@MIP and MH-SiO2@NIP

Adsorbent / Langmuir / Freundlich
Qmax.cal
(mg•g-1) / Qmax.exp
(mg•g-1) / KL ( L•mg-1) / R2 / n / Kf ( mg-(n+1)/n•L1/n•g-1) / R2
MIP / 6.81 / 5.49 / 0.0055 / 0.99785 / 1.99 / 0.2282 / 0.98010
NIP / 1.90 / 1.62 / 0.0054 / 0.97396 / 2.37 / 0.0983 / 0.95907

Table S2 Kinetic parameters of the pseudo-second-order rate equation for allantoin adsorption on MH-SiO2@MIP and MH-SiO2@NIP

Adsorbent / k2
( g•mg−1•min−1) / Qe
( mg•g-1) / h0
( mg•g−1•min−1) / R2
MIP / 0.042 / 5.85 / 1.44 / 0.99582
NIP / 0.022 / 1.79 / 0.07 / 0.99106

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