Supplementary data

Effect of forestry-waste biocharson adsorption ofPb(II) and antibioticFlorfenicolinred soil

Canlan Jiang , HaoCai , Lulu Chen, Liwei Chen, TianmingCai*

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

*Corresponding author: TianmingCai

E-mail: ; Tel: +86 25 84395002, Fax: +86 25 84395002

Table S1 surface area and total pore volume of biochars produced under various temperatures.

Bulk material / Time
h / Temperature
˚C / BET surface area m2 g-1 / Total pore volume (cm3 g−1)
P. massoniana / 1 / 700 / 5.63E+02 / 3.07E-01
P. massoniana / 1 / 450 / 2.24E+02 / 1.07E-01
P. massoniana / 1 / 300 / No detectable / No detectable
C. lanceolata / 1 / 700 / 5.93E+02 / 3.60E-01
C. lanceolata / 1 / 450 / 1.46E+02 / 7.04E-02
C. lanceolata / 1 / 300 / No detectable / No detectable

Text S1

Analysis of biochar characteristics

For pH measurement, after mixing biochar with milli-Q water at the ratio of 1:5 (wt:vol), the mixture was stirred for 2 min using an electromagnetic stirrer. After 1 hour’s equilibrium, the pH of the mixture was measured using a digital pH meter (Sanxin-MP521, Shanghai YouYi Co., China). The carbon (C), nitrogen (N) and hydrogen (H) contents in biochar were measured using an elemental analyzer (Flash EA 1112, ThermoFinnigan, Italy). Oxygen (O) concentration was calculated by assuming that the total weight of biochar was made up of C, H, N and O elements. Scanning electron microscope (SEM) image was recorded on an XL30-ESEM (Philips Co., Netherlands) with the maximum resolution of 3.0 nm and accelerating voltage of 20 kV. Solid-state 13C nuclear magnetic resonance (NMR) spectra of biochar were conducted on a Bruker AV-400 NMR spectrometer (Bruker Inc., Switzerland), equipped with a 4 mm wide-bore MAS probe. The surface are and pore structure of biochar were measured using the nitrogen gas adsorption and desorption method on an Autsorb-1-C analyzer (Quantachrome Instruments, USA). Specifically, the samples were firstly degassed at 300˚C and then measured for at least 5 h whereas the degassing time was changed with the time necessary to reach a stable surface area measurement. The surface areas and total pore volumewere measured over a P/P0 range of 0.05-0.35. In order to characterize the surface functional groups, biochar was investigated by fourier transform infra-red (FTIR) analysis using a Nicolet 5700 (Nicolet Instrument, Thermo, Co., USA). The biochar sample was ground and mixed with 1% KBr and then pressed as a pellet and he spectra were normalized to the highest peaks in the fingerprint region between 4000 and 400 cm-1with a resolution of 4.0 cm-1.

Florfenicol

Log Kow=-0.12

Water sol.=1320 mg L-1

pKa=9.0

Fig S1 chemical structure and physical properties of florfenicol.

Fig S2 Langmuir and Freundlich adsorption isotherms of Pb(II) onto soils.a, Langmuir fitting; b, Freundlich fitting.

Fig.S3 Solid-state 13C nuclear magnetic resonance (NMR) spectra characterizing of the chemistry of the biochars.

Fig S4 Langmuir and Freundlich adsorption isotherms of FLO onto soils. a, Langmuir fitting; b, Freundlich fitting.