Supplementary data

Table

Sup.Table S1.All primers used in the experiments.

Sup.Table S2. Identity betweenec-KIFC1 inEumeces chinensisand other homologues by Vector NTI.

Eriocheir sinensis (ADJ19048.1), Danio rerio (CAD60638.1), Rattus norvegicus (NP_001005878.1), Salmo salar (ABQ59663.1), Mus musculus (BAA19676.1), Homo sapiens (NP_002254.2), Bos Taurus(DAA16684.1), Gallus gallus (NP_001075167.1), Xenopus. Laevis(NP_001081003.1)

Figure legends

Sup.Fig.S1.The ec-KIFC1 nucleotide sequence and deduced amino acid sequence of Eumeces Chinensis. The 2339 bp cDNA consists of a 216 bp 5’untranslated region, a 194bp 3’untranslated region, respectively marked by yellow and red dashed lines. and a 1929 bp open reading frame that encodes the ec-KIFC1protein of 643 amino acids with blue alphabet.Deduced amino acid sequence is demonstrated on above of each line of nucleotides.The initiate codon ATG is highlighted by red frame, and terminal codon TGA is marked by black frame correspond to the “*”.

Sup.Fig.S2.Multiple sequence alignment of Eumeces chinensisKIFC1 protein with its homologues inEriocheir sinensis,Salmo salar, Danio rerio, Xenopus laevis, Musmusculus and Bos Taurus,using Vector NTI10 (Invitrogen).Alignment of KIFC1homologuesfrom several species showed the N-terminal of KIFC1 wasquite divergent while the C-terminal was more conserved. The putative ATP-binding motifs include the AYGQTGSGKT, SSRSH and theLAGSE sequences (labeled red frame) and the microtubule-bindingmotif, YNETIRDLL sequence was labeled by a pink rectangle. The KIFC conserved consensus ELKGN sequence was labeled by a blue rectangle and the RVFCRVRP domain mightrepresent the β1 strand (labeled by a purplerectangle).

Sup.Fig.S3. The phylogenetic analysis of KIFC1 inEumeces chinensisand itshomologues in other species using the neighbour-joining method with Mega5.0.The GenBank accession numbers are as follows: Eriocheir sinensis (ADJ19048.1),Danio rerio (CAD60638.1), Rattus norvegicus (NP_001005878.1), Salmosalar (ABQ59663.1), Mus musculus (BAA19676.1), Homo sapiens(NP_002254.2), Bos Taurus(DAA16684.1), Gallus gallus(NP_001075167.1), Xenopus. Laevis(NP_001081003.1) and Eumeces chinensis(JF274260),D. melanogaster(NP_476651.1).

Supplementary Table 1

Primer name / Primer sequence (5’ to 3’) / Purpose
F1
F2 / GGAAATATCMGGGTNTTYTGYMG ATCTTCGCTTAYGGNCARACNGG / Cloning
cloning
R1 / TCAGAGCCAGCNARRTCNAC / cloing
R2 / CCAATAACACACTCATTCACYTTNSWNGC / cloning
3’RACEF1 / AACTCCTTGGGCGGCAACTCT / 3’RACE
3’RACEF2 / GCGGCAACTCTAAGATGCTGAT / 3’RACE
5’RACER1 / TCCTCCGTGCTCTGGCTCACTCTC / 5’RACE
5’RACER2 / CGCTCGTTGAGGACCGTCTTGG / 5’RACE
Probe-F / CCAGCCAGTAGCGAGAATG / In situhybridization
Probe-R / GCTCTTGCACCGTGTTATGC
RT-F / TGACTTCCACCACCACAGCA / Tissue expression of ec-kifc1
RT-R / AACGCAATCTCCTCAAACACC
Act-F / TGGTATTGTAATGGACTCTGG / β-actin primers for positive control
Act-R / CTTCTCCTTTATGTCACGCAC

Supplementary Table 2

Species name / Identity with ec-KIFC1
Mus musculusKIFC1 / 45.6%
Salmo salarKIFC1 / 42.8%
Danio rerioKIFC1 / 44.6%
Eriocheir sinensis KIFC1 / 36.9%
Rattus norvegicusKIFC1 / 43.7%
Homo sapiensKIFC1 / 46.4%
Bos Taurus KIFC1 / 45.1%
Gallus gallus KIFC1 / 55.6%
Xenopus laevisXCTK2 / 49.8%

Supplementary Fig. 1-1

Supplementary Fig. 1-2

Supplementary Fig 2-1

Supplementary Figure 3