“preliminary Phytochemical and pharmacological investigationOF THE LEAVES OF

PORTULACAQUADRIFIDALINN”

FAMILY: PORTULACACEAE.

M. Pharm Dissertation Protocol

Submitted to the

RajivGandhiUniversity Of Health Sciences, Karnataka, Bangalore

By

WADGEERE VIJAY KUMAR

Under the guidance of

Dr. M.A.HALKAI

M.Pharm., Ph.D

DEPARTMENT OF pharmacognosy

H.K.E.SCOLLEGE OF PHARMACY,

GULBARGA – 585105

2009-10

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES, KARNATAKA

BANGALORE

ANNEXURE – II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1 / Name of the candidate
And address
( in block letters) / : / Wadgeere Vijay Kumar
post: holesamudra, taluqa: aurad
district: bidar. 585417
2 / Name of the Institution / : / H.K.E Society’s College of Pharmacy, Sedam Road,
Gulbarga– 585105
3 / Course of study and subjects / : / Master of Pharmacy pharmacognosy
4 / Date of admission to the course / : / 16.06. 2009
5 / Title of Topic / : / “Preliminary phytochemical and pharmacological investigation on the leaves of Portulaca quadrifidalinn. family: Protulacaceae for anti ulcer activity on albino rats”
6 / Brief Resume of the intended work
6.1 / Need for the study
The literature survey has shown not much of the work is reported of Portulaca-quadrifidaLinn Family: Protulacaceae for phytochemical and pharmacological screening, in this study the extraction and the isolation of the chemical constituents and also the pharmacological screening for its antiulcer property is to be carried out.
Portulaca-quadrifida Linn: belonging to family portulacaceae. It is used traditionally as whole plant in " Kapha" cures low fever, asthma, cough, urinary discharges, inflammations, good for eye disease, skin disease and ulcers(Ayurveda)1.
Portulaca quadrifidais used as urinary disorder, ulcers, cough, dysuria, kidney stone problems, skin diseases2.
6.2 / Review of Literature
Portulaca quadrifida Linn Family Portulacaceae
Kannada : Halibachcheli,Hindi : Chaunlayi, Marathi : Kathechanval, Sanskrit : Laghughonika, Tamil : Sinnaparpukkirai, Telugu : Goddupavili
Pharmacognostic studies of Portulaca-oleracea Linn this paper deals with the microscopic study of Leaf: Stem, and root of Portulaca oleracea, along with the physico-chemical and preliminary phytochemical analysis that were also studied3.
The study was carried out for proving hypoxic nerve protective effect of Portulaca oleracea extract4.
The study was carried out the effect of Portulaca oleracea on morphine dependence in mice, this study that Portulaca oleracea extract can decrease morphine dependences in mice5.
The Study ofPortulaca oleracea Linnhave been carried out for the anti tussive effect of this plant on guinea pigs, the effective concentration of aqueous extracts of Portulaca oleracea causing 50 % reduction of cough number(EC50) was also determined, the result showed significant reduction (P<0.001) in cough number6.
Screening of anti-oxidant activity of Portulaca oleracea was carried out7.
Feeding trails of Portulaca oleraceawere conducted in two groups of Nubian goats that received the plant at daily dose of 5g / kg. BW and ad libitum respectively consumption caused weekness of the fore and hind limbs with inability to stand greenish watery diarrhoea and polyuria, hepatonephropathy and enteritis accompanied by alterations in some blood and serum constituents were also observed, proximate analysis of Portulaca oleraceaIndicated high crude proteins (17.9%) crude fibre (20.3%) fat (5.6%) mg (3.2%) and ca (1.8%)8.
Methanolic extracts of six cultivars of Portulaca oleraceawere analysed for their total phenol content (TPC) using the folin – ciocalteu method the anti oxidant activity was measured using the 1-1 Di phenyl - 2- picryl hydrazyl, ferric – reducing anti oxidant power (FRAP) and β – carotene bleaching (BCB) assays, the TPC value of the common variety POI, was the lowest compared to the ornamental cultivars (PO2- PO6). The BCB assays showed that all cultivars were capable of Inhibiting lipid peroxidation and the Inhibitation power did not correlate with PTC value9.
The effect of the ethanolic extract on the locomotor activity. Threshold to noxious stimulus. Anti convulsant activity and relaxant effects on the skeletal muscle were studied. The extract on Intraperitoneal administration, showed a significant reduction in the locomotor activity in mice, Anti-nociceptive activity in rats using Tail flick method, an Increase in the onset time of pentylenetetrazole - Induced convulsions in mice and muscle relaxant activity in Invitro and Invivo experiments10.
The study was carried out To investigate the effects of the ethanol extracts from Portulaca oleraceaon hypoxia models mice and to elucidate the antihypoxia activity and provide scientific basis for the clinical use of Portulaca oleracea11.
Leaves of Portulaca oleraceahave the potential of regeneration when glown in a distilled water medium. The two heavy metals Se and Hg Individually and in combination affected both shoot and root development. They completely arrested shoot development at all concentrations. However, they produced concentration – dependent changes in the development of roots, which ranged from their complete inhibitation to variation in their Initiation time number length12.
6.3 / Objectives of the study
  1. Extraction and isolation of phytoconstituents of leaves Portulaca quadrifida Linn.
  2. TLC/ HPLC / HPTLC analysis of the extracts and identification of major phytoconstituents.
  3. Screening of Anti ulcer activity using albino rats.

7 / Materials and methods
7.1 / Source of data
The required data will be obtained from:
  • Electronic data,
  • Published Research Paper,
  • Review Article from Journal ,
  • Library of HKE’s College of Pharmacy,Gulbarga.

7.2 / Methods of collection of data (including sampling procedure, if any)
The leaves of Portulaca quadrifida Linn are collected from the Gulbarga district and authenticated at the Department of Botany, GulbargaUniversity, Gulbarga
Extraction:
The Leaves of Portulaca quadrifida Linn are shade dried, powered,The sequential Extraction is carried out using different Solvent system like petroleum ether, benzene, chloroform, ethanol, and distilled water and the extracts are concentrated and stored in refrigerator for carrying out the isolation and identification of phytoconstituents along with the screening of anti ulcers activity using albino rats.
Anti-ulcer activity:
1. Pyloric ligation :
The wistar albino rats will be randomly assigned in to 3 groups of 6 animals each albino rats will be deprived of food for 24 hours care will be taken to avoid coprophagy under light ether anaesthesia, the abdomen will be opened by small middle incision and pyloric end portion of the stomach will be ligated, the animals will be killed 4 hours after the operation under the over dose of anaesthetic ether and the gastric contents were collected. After centrifugation the gastric contents of each animal are individually assessed for volume of gastric secretion free and total acid production will be estimated by titrating against 0.01N Na0H using Topfer’s reagent and phenolphthalein indicator drug will be administered 30 minutes prior to pylorus ligation13.
2. Cold (hypothermic) restraint stress induced ulcer,
3. Delayed healing ulcer.
7.3 / Does the study require any investigation or intervention to be conducted on patients or other humans or animals? if so please describe briefly
YES - ( Albino rats )
7.4 / Has ethical clearance been obtained from your institution in case of 7.3 ?
YES
8. / List of References:
  1. Kirtikar KR.and Basu BD. Indian Medical plants, Vol.I : 240-246.
  2. Dr. Madhavachetty. K; Sivaji K; Tulasirao. K. Flowering plants of cittor district Andhra pradesh India. : 32.
  3. Bagepalli S.A.K et al Pharmcognostical studies of Portulaca-oleraceaLinn. Rev. bras. Farmacogn. Oct / Dec 2008 Vol. 18(4) Joao Pessoa.
  4. Wanyin Wang MB et al. Protective effect of Portulaca-oleracea Linn extracts on hypoxic nerve tissue and its mechanism. Asia Pac J Clin Nutr.2007:16 (Suppl 1 ). : 227 – 233.
  5. Gholamreza Karimi ; Toktam Ziace ; Azam Nazari. Effect of Portulaca-oleraceaLinn. Extracts on the morphine dependence in mice. Iranian Journal of Basic medical sciences. 2008. Vol – 10 (4) : 229 – 232.
  6. Mohammad T.B ; Mohammad. H.B ; Farhad. M ; Anti tussive effect of Portulaca-oleraceaLinn. In guinea pigs. Iranian Journal of Pharmaceutical Research. 2004 .(3) : 187 – 190.
  7. Souri. E ; Amin G ; Farsam H ; Barazandesh T.M. Screening of Antioxidant activity and phenolic content of 24 medicinal plant extracts. Daru. 2008 Vol. 16 (2) : 83 – 87.
  8. Obied.W.A, Mohamoud E.N, Mohamed. O.S.A, Portulaca-oleracea: nutritive composition and clinical pathological effects on Nubian goats. W.A obied et al/ small Ruminant Resarch. 2003, 48, 31-36.
  9. Lim. Y.Y, Quah. E.P.L, antioxidant properties of different cultivars of Portulaca-oleracea. Y.Y. Lim, E.P.L Quah/ food chemistry. 2007.103. 734-740.
  10. Radha krishna.R. et al. Neuropharmacological action of Portulaca-oleracea L v.savita. R Radhakrishnan. et al./ Journal of ethanopharmacology. 2001. 76. 171-176.
  11. Cheng.jie chen, et al. Anti hypoxic activity of the ethanol extract from Portulaca-oleracea in mice. C.j.chen et al/. Journal of ethanopharmacology. 2009. 124. 246-250.
  12. Thangavel.P, shahira sultana A, sbburam.V. Interactive effects of selenium and mercury on the restoration potential of leaves of the medicinal plant. P.Thangavel et al/. The science of the total environment. 1999. 243. 1-8.
  13. Vinod kumar. R ; Mohammed mutahar R K ; Lakshmana M ; Ramprasad N S ; Herbal Heritage Jul – Sept 2009 (3) pg : 124 – 132.

9 / Signature of Candidate / WADGEERE.VIJAY KUMAR
10 / Remarks of guide / The preliminary phytochemical investigation and study antiulcer activity on the leaves of Portulaca quadrifida Linn Family Portulacaceae carried out by the candidate and t4he above study genuinely contributes to the scientific information and hence permitted.
11 / Name and designation of the
11.1 / Guide / Dr. M.A. Halkai
M.Pharm., Ph.D
PROFESSOR and Head
Dept. OF Pharmacognosy,
H.K.E.SCollege of Pharmacy,
Gulbarga - 585105
11.2 / Signature
11.3 / Co- guide (if any)
11.4 / Signature
11.5 / Head of the Department / Dr. M.A. Halkai
M.Pharm., Ph.D
PROFESSOR and Head
Dept. OF Pharmacognosy,
H.K.E.SCollege of Pharmacy,
Gulbarga - 585105
11.6 / Signature
12 / 12.1 / Remarks of the Chairman and Principal
12.2 / Signature