Supplemental Table 9
Expression of hepatotoxicity marker genes (31) as determined by microarray and QRT-PCR in rats administered a single acute dose of acetaminophen.
Gene symbol* / Technique / Fold change / Direction of change12 Hours / 24 Hours
Alox5ap / Microarray / 10.40 / 10.11 / UP
QRT-PCR / 13.05 / 14.64 / UP
Alp1 / Microarray / 29.16 / 64.09 / UP
QRT-PCR / 46.28 / 86.99 / UP
Aplra1 / Microarray / 11.01 / 37.70 / UP
QRT-PCR / 33.49 / 99.67 / UP
Bcl211 / Microarray / 4.40 / 3.95 / UP
QRT-PCR / 2.09 / 14.09 / UP
Ccr1 / Microarray / 11.71 / 18.61 / UP
QRT-PCR / 14.27 / 12.76 / UP
Crisp1d2 / Microarray / 93.00 / 94.00 / UP
QRT-PCR / 229.45 / 214.26 / UP
Ddah1 / Microarray / 11.89 / 14.75 / UP
QRT-PCR / 14.32 / 19.06 / UP
Gng8 / Microarray / 9.54 / 5.24 / UP
QRT-PCR / 10.54 / 6.31 / UP
Ggt1 / Microarray / 13.47 / 42.26 / UP
QRT-PCR / 17.63 / 54.97 / UP
Gprk5 / Microarray / 5.85 / 5.95 / UP
QRT-PCR / 5.26 / 4.09 / UP
Hk3 / Microarray / 11.47 / 20.00 / UP
QRT-PCR / 16.28 / 24.29 / UP
Hspa1a / Microarray / 17.82 / 20.20 / UP
QRT-PCR / 11.79 / 13.12 / UP
Il1r2 / Microarray / 33.49 / 78.16 / UP
QRT-PCR / 44.13 / 108.96 / UP
Il1rap / Microarray / 10.3 / 20.26 / UP
QRT-PCR / 16.49 / 21.34 / UP
Ltb4r / Microarray / 35.27 / 88.01 / UP
QRT-PCR / 31.87 / 71.95 / UP
Mmp9 / Microarray / 8.72 / 29.63 / UP
QRT-PCR / 10.25 / 41.31 / UP
Nos2 / Microarray / 10.13 / 11.91 / UP
QRT-PCR / 19.93 / 22.54 / UP
Socs3 / Microarray / 23.65 / 24.87 / UP
QRT-PCR / 24.19 / 21.22 / UP
Tgm1 / Microarray / 75.25 / 157.43 / UP
QRT-PCR / 80.60 / 168.38 / UP
Cd3d / Microarray / 4.40 / 2.77 / DOWN
QRT-PCR / 3.00 / 2.08 / DOWN
Cd79b / Microarray / 3.80 / 5.67 / DOWN
QRT-PCR / 2.65 / 4.64 / DOWN
Cd86 / Microarray / 2.64 / 2.77 / DOWN
QRT-PCR / 2.19 / 2.43 / DOWN
Eno3 / Microarray / 2.65 / 9.28 / DOWN
QRT-PCR / 5.27 / 10.53 / DOWN
Il9r / Microarray / 3.16 / 5.41 / DOWN
QRT-PCR / 2.48 / 5.59 / DOWN
Jup / Microarray / 3.75 / 3.99 / DOWN
QRT-PCR / 2.56 / 2.63 / DOWN
Lat / Microarray / 3.72 / 3.32 / DOWN
QRT-PCR / 2.46 / 2.89 / DOWN
Nrp1 / Microarray / 4.50 / 16.22 / DOWN
QRT-PCR / 2.79 / 7.44 / DOWN
Ptgds2 / Microarray / 3.58 / 4.34 / DOWN
QRT-PCR / 2.81 / 4.04 / DOWN
Rnase6 / Microarray / 13.42 / 20.77 / DOWN
QRT-PCR / 9.14 / 17.63 / DOWN
RT1-DOa / Microarray / 1.84 / 3.13 / DOWN
QRT-PCR / 2.26 / 3.50 / DOWN
St8sia1 / Microarray / 5.22 / 3.61 / DOWN
QRT-PCR / 2.73 / 2.44 / DOWN
* More details about the genes including their full nomenclature and gene bank accession number are available at (Accession number GPL2996).
UP – Up-regulation of expression; DOWN – Down regulation of expression.
Data represents the mean of four biological replicates and only the results of 12- and 24-hour samples are presented.
Fold change in expression is in comparison to the vehicle administered, control rats.
All listed genes are significantly differentially expressed (p0.05 and a fold change 1.8) in the AP administered rats compared with the controls.
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