MALDI FTICR IMS of Intact Proteins: Using Mass Accuracy to Link Protein Images with Proteomics

MALDI FTICR IMS of intact proteins: Using mass accuracy to link protein images with proteomics data

Jeffrey M. Spraggins1,2, David Rizzo2,3, Jessica L. Moore2,3, Kristie L. Rose1,2, Neal D. Hammer4, Eric P. Skaar4,5 and Richard M. Caprioli1,2,3,5

1Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

2Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN USA

3Department of Chemistry, Vanderbilt University, Nashville, TN USA

4Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA

5United States (U.S.) Department of Veterans Affairs, Tennessee Valley Healthcare System

6Departments of Pharmacology and Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA

Address reprint requests to Jeffrey Spraggins, 465 21st Ave S. Room 9160, Nashville, TN 37232, Phone: +1 (615) 343-7333, fax: +1 (615) 343 - 8372,

Supplementary Material

Supplemental Material Figure 1.ETD LC-MS/MS data for ATP synthase subunit epsilon. The charge state distribution is shown in the top panel with the specific charge state selected for MS/MS highlighted in red. ETD fragmentation data is shown in the bottom panel including selected c (red) and z (blue) ion annotations. A summary of the observed fragments and sequence coverage is also included.

Supplemental Material Figure 2.ETD LC-MS/MS data for N-terminally acetylated thymosin β10. The charge state distribution is shown in the top panel with the specific charge state selected for MS/MS highlighted in red. ETD fragmentation data is shown in the bottom panel including selected c (red) and z (blue) ion annotations. A summary of the observed fragments and sequence coverage is also included.

Supplemental Material Figure 3.ETD LC-MS/MS data for Histone H4 (N-acetylated, K21 dimethylated). The charge state distribution is shown in the top panel with the specific charge state selected for MS/MS highlighted in red. ETD fragmentation data is shown in the bottom panel including selected c (red) and z (blue) ion annotations. A summary of the observed fragments and sequence coverage is also included.

Supplemental Material Figure 4.ETD LC-MS/MS data for Histone H4 (N-acetylated, K17 acetylated, K21 dimethylated). The charge state distribution is shown in the top panel with the specific charge state selected for MS/MS highlighted in red. ETD fragmentation data is shown in the bottom panel including selected c (red) and z (blue) ion annotations. A summary of the observed fragments and sequence coverage is also included.

Supplemental Material Figure 5.ETD LC-MS/MS data for S100A8. The charge state distribution is shown in the top panel with the specific charge state selected for MS/MS highlighted in red. ETD fragmentation data is shown in the bottom panel including selected c (red) and z (blue) ion annotations. A summary of the observed fragments and sequence coverage is also included.