ELECTRONIC SUPPLEMENTARY MATERIAL

The evolution of wing shape in ornamented-winged damselflies (Calopterygidae, Odonata)

Evolutionary Biology

David Outomuro, Dean C. Adams and Frank Johansson

Corresponding author: David Outomuro; Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, S-75236, Sweden

e-mail:

Supplementary table 1. Taxa used in the present study to construct the phylogenetic tree and accession numbers in GenBank. Resultant tree was pruned of those taxa for which we did not have samples (marked with an asterisk).

taxa / GenBank accession #
Archineura hetaerinoides * / AJ746327
Archineura incarnata / AJ459202
Atrocalopteryx atrata / Y12889; AJ458982
Atrocalopteryx melli * / JQ341026
Atrocalopteryx oberthueri * / JQ341025
Caliphaea angka* / AJ459219
Caliphaea confusa / AJ459220
Calopteryx aequabilis / Y12888; AJ308360
Calopteryx amata / AJ458977; AJ308361
Calopteryx cornelia / Y12890; AJ458981
Calopteryx exul / Y12891; AJ308346
Calopteryx haemorrhoidalis / Y12892; AJ308347
Calopteryx japonica altaica * / AJ459193
Calopteryx japonica japonica * / Y12893; AJ458980
Calopteryx maculata / AJ459198
Calopteryx splendens ancilla * / AJ459189
Calopteryx splendens balcanica * / AJ308363
Calopteryx splendens caprai * / AJ458967; AJ308369
Calopteryx splendens cretensis * / AJ308364
Calopteryx splendens johanseni * / AJ459188
Calopteryx splendens splendens / AJ458965; AJ308368
Calopteryx splendens taurica * / Y12894; AJ458979
Calopteryx syriaca * / AJ459190
Calopteryx virgo festiva * / AJ459196
Calopteryx virgo meridionalis / AJ458969; AJ308357
Calopteryx virgo padana * / AJ308356
Calopteryx virgo virgo / AJ458968; AJ308359
Calopteryx xanthostoma / AJ458973; AJ308355
Echo modesta / Y12895; AJ458984
Hataerina americana / Y12896; AJ458989
Hetaerina medinai * / AJ459229
Hetaerina titia / Y12897; AJ458990
Iridictyon myersi * / AJ459227
Matrona basilaris / AJ459203
Matrona cyanoptera * / AJ459205
Matronoides cyanipennis / AJ459201
Mnais andersoni / AJ459211
Mnais costalis / AB125622, AB125623
Mnais gregoryi * / JQ341028
Mnais mneme / AJ459213
Mnais pruinosa / Y12898; AJ458985
Mnais tenuis / AJ459212
Mnais yunosukei * / AJ459214
Neurobasis chinensis / AJ459200
Neurobasis longipes * / JQ341024
Noguchiphaea yoshikoae * / AJ459228
Phaon camerunensis / FN356144
Phaon iridipennis / AJ459225
Phaon sp. from Madagascar / AJ459226
Psolodesmus mandarinus dorothea / AJ459207
Psolodesmus mandarinus kuroiwae * / AJ459209
Psolodesmus mandarinus mandarinus * / AJ459208
Sapho bicolor / AJ459222
Sapho ciliata / Y12903; AJ458987
Sapho gloriosa / AJ459221
Sapho puella * / FN356180
Umma longistigma / AJ459224
Umma saphirina / AJ459223
Vestalaria velata * / JQ341021
Vestalis amaryllis * / JQ341022
Vestalis amoena / AJ459215
Vestalis anne * / AJ459216
Vestalis atropha * / JQ341023
Vestalis beryllae * / JQ341027
Vestalis gracilis / Y12901; AJ458986
Vestalis lugens / AJ459218
Vestalis smaragdina * / AJ459217
Euphaea impar (outgroup) / AJ746322
Platycnemis pennipes (outgroup) / AJ459230
Aeshna cyanea (outgroup) / FN356031

Supplementaryfigure 1. Relationship between wing shape and centroid size in front- and hind wings. Wing shape is expressed as the shape scores (see Drake and Klingenberg, 2008). Each colour corresponds to a pigmentation group (see main text): red: group 1; yellow: group 2; blue: group 3; green: group 4; orange: group 5; white: group 6.

SupplementaryR script: R script for PGLS of shape (spp.shape) vs. centroid size (spp.Csize).

##MULTIVARIATE PLGS FUNCTION

mult.pgls<-function(phy,y.mat,x.mat){

phy.mat<-vcv(phy)

x.mat<-cbind(matrix(1,length(phy$tip.label)),x.mat)

x.mat<-x.mat[rownames(phy.mat),]

y.mat<-y.mat[rownames(phy.mat),]

mDnew<-solve(svd(phy.mat)$u%*%diag(sqrt(svd(phy.mat)$d))%*%t(svd(phy.mat)$u))

ynew<-mDnew %*% y.mat

xnew<-mDnew %*% x.mat

summary=summary(manova(lm(ynew~xnew-1)))

return(list(summary=summary))

}

#ANALYSIS

mult.pgls(tree,spp.shape,spp.Csize)

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