Supplemental Table S4. Relative quantification of ICHO/ICOOH derivatives in rosette leaves of wild-type (Col-0) and mutant plants.
Rosette leaves of 16-18 plants per genotype (cultivated in two independent experiments) were individually pooled, extracted and the resulting extracts analysed by UPLC/ESI-QTOFMS. Metabolites were quantified using extracted ion chromatograms (for quantifier ions see Table 1). The resulting peak areas were normalized to fresh weight, log2-transformed and centred individually for each independent experiment to the median of the normalized and log2-transformed peak areas of wild-type (Supplemental Table S3). Significance analysis of differences between wild-type and mutant was performed by t-test (two-tailed, unequal variances, **P 10e-03, ***P 10e-04). Significant differences are marked in bold.
genotype / log2 [response / medianresponse Col-0] (mediansd, n = 16-18)Col-0 / cyp71B6-1 / cyp71B6-2 / CYP71B6-OE#2 / CYP71B6-OE#4 / aao1 / AAO1-OE#7
ICOOGlc / 0.00 0.87 / -0.09 0.90 / -0.02 0.51 / -0.04 0.58 / 0.13 0.63 / -0.11 0.67 / 0.71 0.80
6-GlcO-ICOOH / 0.00 0.66 / -0.03 0.55 / -0.12 0.40 / -0.17 0.42 / 0.05 0.32 / -0.36 0.34 / 0.30 0.74
6-GlcO-ICOOGlc / 0.00 0.65 / -0.18 0.56 / -0.20 0.46 / -0.21 0.39 / -0.07 0.26 / -0.42 0.36 / 0.14 0.81
5-GlcO-ICHO / 0.00 0.38 / -0.31 0.39 / 0.18 0.40 / 0.12 0.29 / 0.17 0.20 / 0.75 0.29*** / -0.92 0.91**
4-GlcO-ICHO / 0.00 0.70 / 0.18 0.70 / -0.19 0.37 / -0.07 0.41 / 0.10 0.52 / 0.14 0.43 / 0.04 0.72
DihydroascHexoside / 0.00 0.65 / 0.08 0.59 / 0.04 0.50 / 0.05 0.36 / 0.11 0.37 / -0.04 0.44 / 0.15 0.74