Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis

Journal of Materials Science: Materials in Medicine

Bérengère J.C. Luthringer1*, Uma M.R. Katha2, and Regine Willumeit1

1 Institute of Materials Research, Department for Structural Research on Macromolecules, Helmholtz-Zentrum Geesthacht (HZG), Geesthacht, Germany

2 GALAB Laboratories GmbH, Hamburg, Germany

* Please address correspondence to: Dr. Bérengère Luthringer; E-mail:

Supplementary material: Real-time PCR primers. For each gene official gene name and abbreviation (human genome organisation, HUGO) and corresponding trivial synonym (in italic), accession number (from national centre for biotechnology information (NCBI) reference sequence collection database), primers, and amplicon size are indicated

Target name / NCBI RefSeq
Abbreviation / Forward / Reverse / Amplicon length
[bp]
Alkaline phosphatase, liver/bone/kidney / NM_000478
ALPL / CACCCACGTCGATTGCATCT / TAGCCACGTTGGTGTTGAGC / 211
Progressive ankylosis protein homolog / NM_054027.4
ANKH / GTTCACCTTCGTCTGCATGGCT / GGCAAAGTCCACTCCGATGATG / 109
Activating transcription factor 4 (tax-responsive enhancer element B67) / NM_001675.2
ATF4 / AGTGGCATCTGTATGAGCCCA / GCTCCTATTTGGAGAGCCCCT / 81
Beta-2-microglobulin / NM_004048
B2M / TGCTGTCTCCATGTTTGATGTATCT / TCTCTGCTCCCCACCTCTAAGT / 86
BCL2-associated X protein / NM_004324
BAX / GGGTGGTTGGGTGAGACTC / AGACACGTAAGGAAAACGCATTA / 191
BCL2B-cell CLL/lymphoma 2 / NM_000657.2
BCL2 / GGATTGTGGCCTTCTTTG / TACCCAGCCTCCGTTATC / 152
Bone morphogenetic protein 1 / NM_006129
BMP1 / GGGTCATCCCCTTTGTCATTG / GCAAGGTCGATAGGTGAACACA / 152
Bone morphogenetic protein 2 / NM_001200
BMP2 / ACCCGCTGTCTTCTAGCGT / CTCAGGACCTCGTCAGAGGG / 140
Bone morphogenetic protein 4 / NM_001202
BMP4 / ATGATTCCTGGTAACCGAATGC / CCCCGTCTCAGGTATCAAACT / 93
Bone morphogenetic protein 6 / NM_001718
BMP6 / AGCGACACCACAAAGAGTTCA / GCTGATGCTCCTGTAAGACTTGA / 159
CD44 molecule (Indian blood group); Hyaluronate receptor / NM_001001389.1
CD44 / AGGCTGGGAGCCAAATGAAG / TGTGGTCAAAAGCCCGTGG / 131
Cadherin-11; Osteoblast-cadherin / NM_001797
CDH11 / AACCTGAGTATCAGTACATGCCT / CCTGCCTTCATAACCGTAGATTT / 155
Collagen, type I, alpha 1 / NM_000088
COL1A1 / AAGACATCCCACCAATCACC / GCAGTTCTTGGTCTCGTCAC / 159
Distal-less homeobox 5/ NM_005221
DLX5 / GAGTGTTTGACAGAAGGGTCC / GAATCGGTAGCTGAAGACTCG / 127
Ectonucleotide pyrophosphatase/phosphodiesterase 1 / NM_006208
ENPP1 / TTGGACCCTCAGTGGCAACTTG / TGCCATGCTTGAATCCAGGTCC / 136
Fibrillin 1 / NM_000138
FBN1 / AAAGGGGGTTTGTACCTTGG / AGGTGCAGCGTTAGAAGGAA / 67
Fibronectin 1 / NM_212474
FN1 / AGTGGACACCACCCAATGTT / TTCATTGGTCCGGTCTTCTC / 76
FOS-like antigen 1 / NM_005438
FOSL1 / ATTTCCCATTTGTGCCAGAG / CAGGAAGAGGGTGATGGAGA / 118
Glyceraldehyde-3-phosphate dehydrogenase / NM_002046
GAPDH / GTCGGAGTCAACGGATTTG / TGGGTGGAATCATATTGGAA / 143
Intercellular adhesion molecule 1 / NM_000201
ICAM1 / TCTGTGTCCCCCTCAAAAGTC / GGGGTCTCTATGCCCAACAA / 101
Integrin, alpha 1; CD49a; Laminin and collagen receptor / NM_181501
ITGA1 / GTGCTTATTGGTTCTCCGTTAGT / GCCCACAAGCCAGAAATCCT / 211
Integrin, alpha 2; CD49b; / NM_002203
ITGA2 / TGTTGTTTGGCCTACAATGTTGG / CAACCAGTAACCAGTTGCCTT / 121
Integrin, alpha V; CD51; vitronectin receptor / NM_002210
ITGAV / ATCTGTGAGGTCGAAACAGGA / TCCAATGGTACAATGGGGCAC / 56
Mitogen-activated protein kinase 3 / NM_002746
MAPK3 / CTACACGCAGTTGCAGTACAT / CTACACGCAGTTGCAGTACAT / 157
Bone gamma-carboxyglutamate (gla) protein, Osteocalcin / NM_199173.4
BGLAP, OC / ATGAGAGCCCTCACACTCCT / TGGACACAAAGGCTGCAC / 115
Tumor necrosis factor receptor superfamily, member 11b, Osteoprotegerin / NM_002546.3
TNFRSF11B
OPG / CGCTCGTGTTTCTGGACAT / GGACATTTGTCACACAACAGC / 112
Secreted phosphoprotein 1, Osteopontin / NM_000582
SPP1, OPN / CTCCATTGACTCGAACGACTC / CAGGTCTGCGAAACTTCTTAGAT / 60
Phosphatase, orphan 1, Phosphoethanolamine/phosphocholine phosphatase / NM_001143804.1
PHOSPHO1 / GACGAAAACAGCGACGATTC / CGGAGATGAGAATCACCTCG / 253
Solute carrier family 20 (phosphate transporter), member 1 / NM_005415.4
PIT1 / GGAAACCAGCATAGATAGCACCG / CACGGTGTGATACTGGTAGTGG / 124
Polymerase (RNA) II (DNA directed) polypeptide A / NM_000937
POLR2A / GAGAGCGTTGAGTTCCAGAACC / TGGATGTGTGCGTTGCTCAGCA / 152
Tumour necrosis factor receptor superfamily, member 11a, Receptor activator of nuclear factor kappa B / NM_003839.3
TNFRSF11A
RANK / TTTACTGGCCACAAATGCCC / TCCTAATCGACCGCTACCAC / 96
Tumour necrosis factor ligand superfamily, member 11, Receptor activator of nuclear factor kappa B ligand / NM_003701.3
TNFSF11
RANKL / AGAGCGCAGATGGATCCTAA / TTCCTTTTGCACAGCTCCTT / 180
Runt-related transcription factor 2 / NM_001015051
RUNX2 / r CAGTAGATGGACCTCGGGAA / ATACTGGGATGAGGAATGCG / 112
Sp7 transcription factor, Osterix / NM_152860
SP7 / TTCTGCGGCAAGAGGTTCACTC / GTGTTTGCTCAGGTGGTCGCTT / 129
Secreted protein, acidic, cysteine-rich; Osteonectin / NM_003118
SPARC / GAGAAGGTGTGCAGCAATGA / AAGTGGCAGGAAGAGTCGAA / 50
Transforming growth factor, beta 1 / NM_000660
TGFB1 / CAAGCAGAGTACACACAGCAT / TGCTCCACTTTTAACTTGAGCC / 129
Tumour necrosis factor / NM_000594
TNFA / ATGAGCACTGAAAGCATGATCC / GAGGGCTGATTAGAGAGAGGTC / 217
Tumour necrosis factor receptor superfamily, member 1A / NM_001065
TNFRSF1A / TTGCATCCTAGCCCAGCAG / CTGACCCTGGAACAGAAAAGTC / 211
Vinculin / NM_003373
VCL / TGTAGCCTTCCTCCACATCC / AGGGGACAAGCACTCCTCTT / 121
Vitronectin / NM_000638
VTN / TGACCAAGAGTCATGCAAGGG / CAGCCGTATAGTCTGTGCAGC / 150