Supplementary Material Table I. Primers for HS amplicons
RE / Sequence / Position Primer / Positions enzyme / Amplicon size / Fragment size
HpaII / CTC CTT ATT CCT TTC CTC / 317049 / 317383 / 340428 / 640 / 23045
CAA GAA TTG GGA AGT CAC AGC ACT C / 317689
PstI / GCC TCT TCA AAA CAC TCT CC / 324091 / 318658 / 324461 / 513 / 5803
TGC CTG CTG CAT TCA TGT AG / 324604
HpaI / GAT GGA ACT CTC TGT ATC CAG / 321278 / 321055 / 326200 / 643 / 5145
GTT GGA CAA CCT CTT CAC AC / 321921
BamHI / CCT GCC AGT TTC TCC CAA ATC TAC / 337377 / 330287 / 338145 / 734 / 7858
GCT CAG AGA TAC TTG GGA AG / 338111
PflFI / AGG CTG ATG GCC AAG CTT AAG / 357008 / 345263 / 357531 / 554 / 12268
CTC CTG TTC TTG GGT AAC TAG / 357562
AflII / AGG CTG ATG GCC AAG CTT AAG / 357008 / 357024 / 370709 / 554 / 13685
CTC CTG TTC TTG GGT AAC TAG / 357562
AflII / AAG AAA CAG GCT CGA CCA AAA C / 369823 / 357024 / 370709 / 695 / 13685
CAT TGA AGC AGA GTC TGA ATG G / 370518
XmaI / CCA GAT CCA ACG TGA TTA GCT G / 378311 / 364499 / 379306 / 172 / 14807
ATA GCC TCC CAC ACT CCT TTA G / 378483
SspI / AAG TGA AGC CTC CAT AGC TC / 365022 / 364894 / 370950 / 498 / 6056
GTG TTT TCT CTT CCC ATC CAG C / 365520
SspI / AAG AAA CAG GCT CGA CCA AAA C / 369823 / 364894 / 370950 / 695 / 6056
CAT TGA AGC AGA GTC TGA ATG G / 370518
StuI / TCT TGC TTG TGG CTA ACC AC / 393911 / 384690 / 394523 / 607 / 9833
TCC TTT GGA CTC TGT GAC AC / 394518
EcoRV / CCT GCT TGC CTT AGT CCA TAT C / 394258 / 394222 / 406741 / 603 / 12519
TCT GGC TAG TCT GGA GTT CAA G / 394861