Supplementary table S1. Protein changes during germination of WT and PaSOD expressing Arabidopsis seeds under copper stress. From 2D protein profiles of WT and transgenic Arabidopsis seeds, differentially expressed protein spots were extracted, tryptic digested and analyzed with MALDI. This data used for PMF analysis (BLAST) against the existing database (NCBI).
Spot
No. / Maximum Homology with (Protein Name) / Organism / ExptMW/
Theor.
MW /
Expt
PI/ Theor.PI /
No. of queries matched
/Sequence coverage (%)
/Score
/Accession No.
/Function
2 / Plastid envelope DNA binding protein /P. sativum
/ 69/69.4 / 4.7/4.7 / 10 / 23 / 65 / CAA67292 / Nucleoid anchoring (replication in developing plastids)4 / Ethylene-overproduction protein 1 /
A. thaliana
/ 68/95 / 4.6/5.3 / 12 / 20 / 60 / O65020 / Essential regulator of the ethylene pathway8 / Vacuolar invertase /
N. tobacum
/ 62/71.2 / 5.1/5.4 / 8 / 24 / 66 / CAC83577 / Sucrose cleavage11 / ATMYB1 (MYB DOMAIN PROTEIN 1)DNA binding / transcription factor /
A. thaliana
/ 34/42.6 / 3.8/5.3 / 3 / 19 / 53 / NP_187534 / DNA binding / transcription factor13 / Oxidoreductase, zinc-binding dehydrogenase family protein /
A. thaliana
/ 44/40.84 / 7/8.46 / 5 / 22 / 69 / NP_173786 / Energy production and conversion14 / F1L3.35 (hypothetical protein) /
A. thaliana
/ 41/41.4 / 8.0/8.9 / 5 / 16 / 50 / AAF79475 / Lipid metabolism16 / Cysteine synthase;
O-acetylserine (thiol) lyase /
A. thaliana
/ 32/32.3 / 6.8/5.8 / 10 / 32 / 60 / BAB01461 / Amino acid transport andmetabolism
18 / Unknown protein /
A. thaliana
/ 31/31.93 / 7.0/9.02 / 8 / 40 / 65 / NP_198167 / Unknown function19 / Phenazine biosynthesis-like protein (putative) /
A. thaliana
/ 30/32.1 / 5.6/5.27 / 3 / 17 / 52 / AAM20496 / PhzC/PhzF is involved in dimerisation of two 2,3-dihydro-3-oxo-anthranilic acid molecules to create PCAby P. fluorescens
20 / GS1-like protein /
A. thaliana
/ 27/27.5 / 7.5/5.6 / 4 / 17 / 53 / AAM67188 / Predicted phosphatase/phosphohexomutase22 / Quinone oxidoreductase-like protein /
A. thaliana
/ 25/32.7 / 7/5.65 / 5 / 24 / 64 / AAM62737 / NADPH:quinone reductase and related Zn-dependent oxidoreductases25 / β-cruciferin (12S seed storage protein CRB precursor) /
A. thaliana
/ 20/20.7 / 6.5/6.36 / From Arabidopsis seed proteome / - / From Arabidopsis seed proteome / P15456 / seed storage protein27 / Lipase class 3 family potein /
A. thaliana
/ 65/72.9 / 4.1/8.85 / 16 / 27 / 60 / NP_567131 / Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface28 / Putative polyprenyl diphosphate synthase / O. sativa / 35/44 / 5.2/5.7 / 6 / 27 / 58 / NM_001062973.1 / Metabolic condensation reactions
32 / 26S proteasome AAA-ATPase subunit RPT5a / A. thaliana / 40/47.4 / 7.1/4.95 / 9 / 36 / 59 / AAN15459 / AAA-super family of ATPases associated with a wide variety of cellular activities, including membrane fusion, proteolysis, and DNA replication
33 / Unknown protein / A. thaliana / 32/21.5 / 8.0/9.32 / 7 / 46 / 67 / AAS47606 / Unknown function
34 / Glycoprotein-specific UDP-glucuronyltransferase-like protein / A. thaliana / 25/35.5 / 6.5/7.14 / 9 / 38 / 56 / AAM64331 / Metabolism
35 / Hypothetical protein / O. sativa / 21/19.7 / 7.5/10.5 / 5 / 42 / 54 / AP003626.3 / Unknown function
36 / Unknown protein / A. thaliana / 20/19.07 / 8.1/9.86 / 7 / 29 / 50 / NP_850326 / Unknown function
37 / Unknown protein / A. A. thaliana / 18/19.3 / 7.8/8.93 / 5 / 30 / 44 / AAM62817 / The function of these proteins is unknown but might be an Fe-S cluster as part of an
oxidoreductase complex
38 / MADS-box protein AGL27-I / A. thaliana / 12.0/19.7 / 9.0/6.02 / 4 / 49 / 47 / AAG37902 / DNA binding Transcription factor
39 / Heat shock protein HSP26 / T. aestivium / 21/26.8 / 9.8/7.85 / 7 / 13 / 51 / AAB28590 / Molecular chaperone