CURRICULUM VITAE

Prof. Massimiliano COLETTA

Born in Panicale (PG) on April 26, 1951. Graduated in Medicine on March 15, 1977 at the University of Roma "La Sapienza". Spent the period 1979-1981 as a Fogarty International Fellow at the Lab. of Chem. Phys. of the Nat. Inst. of Health, Bethesda, USA. Research Scientist of the National Research Council of Italy over 1982-1988. Associate Professor of Biochemistry over 1988-1990 at the University of Roma "La Sapienza". Full Professor of Molecular Biology over 1990-1996 at the University of Camerino. Presently, Full Professor of Biochemistry at the Faculty of Medicine, University of Roma Tor Vergata.

Scientific Topics:

-Kinetics and Thermodynamics of Protein-Ligand Interactions in Hemoproteins involved in Electron Transfer and Gas Transport.

-Steady- and Presteady State Kinetics of Proteolytic Enzymes, with special reference to MetalloProteinases involved in Inflammatory Responses and Tumoral Progression.

CAO Y, NICOLETTI FP, DE SANCTIS G, BOCEDI A, CIACCIO C, GULLOTTA F, FANALI G, TUNDO GR, DI MASI A, FASANO M, SMULEVICH G, ASCENZI P, COLETTA M (2012). Evidence for pH-dependent multiple conformers in iron(II) heme-human serum albumin: spectroscopic and kinetic investigation of carbon monoxide binding. JBIC, vol. 17, p. 133-147

SBARDELLA D, FASCIGLIONE GF, GIOIA M, CIACCIO C, TUNDO GR, MARINI S, COLETTA M (2012). Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes. . MOLECULAR ASPECTS OF MEDICINE, vol. 33, p. 119-208

ASCENZI P, POLTICELLI F, MARINO M, SANTUCCI R, COLETTA M (2011). Cardiolipin drives cytochrome c proapoptotic and antiapoptotic actions. IUBMB LIFE, vol. 63, p. 160-165

ASCENZI P, CIACCIO C, SINIBALDI F, SANTUCCI R, COLETTA M (2011). Cardiolipin modulates allosterically peroxynitrite detoxification by horse heart cytochrome c. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 404, p. 190-194

GRASSO G, PIETROPAOLO A, SPOTO G, PAPPALARDO G, TUNDO GR, CIACCIO C, COLETTA M, RIZZARELLI E (2011). Copper(I) and copper(II) inhibit Aβ peptides proteolysis by insulin-degrading enzyme differently: implications for metallostasis alteration in Alzheimer's disease. CHEMISTRY-A EUROPEAN JOURNAL, vol. 17, p. 2752-2762

FERRARIS DM, SBARDELLA D, PETRERA A, MARINI S, AMSTUTZ B, COLETTA M, SANDER P, RIZZI M (2011). Crystal Structure of Mycobacterium tuberculosis Zinc-dependent Metalloprotease-1 (Zmp1), a Metalloprotease Involved in Pathogenicity. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, p. 32475-32482

P. ASCENZI, Y. CAO, G.R. TUNDO, COLETTA M, G. FANALI AND M FASANO (2011). Ibuprofen and warfarin modulate allosterically ferrous human serum heme-albumin nitrosylation.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 411, p. 185-189

ASCENZI P, BOLLI A, DI MASI A, TUNDO GR, FANALI G, COLETTA M, FASANO M (2011). Isoniazid and rifampicin inhibit allosterically heme binding to albumin and peroxynitrite isomerization by heme-albumin. JBIC, vol. 16, p. 97-108

GIORDANO D, RUSSO R, CIACCIO C, HOWES BD, DI PRISCO G, MARDEN MC, HUI BON HOA G, SMULEVICH G, COLETTA M, VERDE C (2011). Ligand- and proton-linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125. IUBMB LIFE, vol. 63, p. 566-573

MANCINO R, DI PIERRO D, VARESI C, CERULLI A, FERACO A, CEDRONE C, PINAZO-DURAN MD, COLETTA M, NUCCI C (2011). Lipid peroxidation and total antioxidant capacity in vitreous, aqueous humor, and blood samples from patients with diabetic retinopathy. MOLECULAR VISION, vol. 17, p. 1298-1304

ASCENZI P, GULLOTTA F, GIOIA M, COLETTA M, FASANO M (2011). O(2)-mediated oxidation of ferrous nitrosylated human serum heme-albumin is limited by nitrogen monoxide dissociation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 406, p. 112-116

ASCENZI P, CIACCIO C, SINIBALDI F, SANTUCCI R, COLETTA M (2011). Peroxynitrite detoxification by horse heart carboxymethylated cytochrome c is allosterically modulated by cardiolipin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 415, p. 463-467

PESCE A, TILLEMAN L, DEWILDE S, ASCENZI P, COLETTA M, CIACCIO C, BRUNO S, MOENS L, BOLOGNESI M, NARDINI M (2011). Structural heterogeneity and ligand gating in ferric methanosarcina acetivorans protoglobin mutants. IUBMB LIFE, vol. 63, p. 287-294

HOWES BD, GIORDANO D, BOECHI L, RUSSO R, MUCCIACCIARO S, CIACCIO C, SINIBALDI F, FITTIPALDI M, MARTÍ MA, ESTRIN DA, DI PRISCO G, COLETTA M, VERDE C, SMULEVICH G (2011). The peculiar heme pocket of the 2/2 hemoglobin of cold-adapted Pseudoalteromonas haloplanktis TAC125. JBIC, vol. 16, p. 299-311

ASCENZI P, SANTUCCI R, COLETTA M, POLTICELLI F (2010). Cytochromes: Reactivity of the "dark side" of the heme. BIOPHYSICAL CHEMISTRY, vol. 152, p. 21-27

SINIBALDI F, HOWES BD, PIRO MC, POLTICELLI F, BOMBELLI C, FERRI T, COLETTA M, SMULEVICH G, SANTUCCI R (2010). Extended cardiolipin anchorage to cytochrome c: a model for protein-mitochondrial membrane binding. JBIC, vol. 15, p. 689-700

MAZZUCA C, ORIONI B, COLETTA M, FORMAGGIO F, TONIOLO C, MAULUCCI G, DE SPIRITO M, PISPISA B, VENANZI M, STELLA L (2010). Fluctuations and the rate-limiting step of peptide-induced membrane leakage. BIOPHYSICAL JOURNAL, vol. 99, p. 1791-1800

ASCENZI P, DI MASI A, GULLOTTA F, MATTU M, CIACCIO C, COLETTA M (2010). Reductive nitrosylation of ferric cyanide horse heart myoglobin is limited by cyanide dissociation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 393, p. 196-200

ASCENZI P, CAO Y, DI MASI A, GULLOTTA F, DE SANCTIS G, FANALI G, FASANO M, COLETTA M (2010). Reductive nitrosylation of ferric human serum heme-albumin. THE FEBS JOURNAL, vol. 277, p. 2474-2485

GIOIA M, FASCIGLIONE GF, MONACO S, IUNDUSI R, SBARDELLA D, MARINI S, TARANTINO U, COLETTA M (2010). pH dependence of the enzymatic processing of collagen I by MMP-1 (fibroblast collagenase), MMP-2 (gelatinase A), and MMP-14 ectodomain. JBIC, vol. 15, p. 1219-1232

DE MARINIS E, CASELLA L, CIACCIO C, COLETTA M, VISCA P, ASCENZI P (2009). Catalytic peroxidation of nitrogen monoxide and peroxynitrite by globins. IUBMB LIFE, vol. 61, p. 62-73

GEMMA S, CAMPIANI G, BUTINI S, JOSHI BP, KUKREJA G, COCCONE SS, BERNETTI M, PERSICO M, NACCI V, FIORINI I, NOVELLINO E, TARAMELLI D, BASILICO N, PARAPINI S, YARDLEY V, CROFT S, KELLER-MAERKI S, ROTTMANN M, BRUN R, COLETTA M, MARINI S, GUISO G, CACCIA S, FATTORUSSO C (2009). Combining 4-aminoquinoline- and clotrimazole-based pharmacophores toward innovative and potent hybrid antimalarials. JOURNAL OF MEDICINAL CHEMISTRY, vol. 52, p. 502-513

BOZZI M, INZITARI R, SBARDELLA D, MONACO S, PAVONI E, GIOIA M, MARINI S, MORLACCHI S, SCIANDRA F, CASTAGNOLA M, GIARDINA B, BRANCACCIO A, COLETTA M (2009). Enzymatic processing of beta-dystroglycan recombinant ectodomain by MMP-9: identification of the main cleavage site. IUBMB LIFE, vol. 61, p. 1143-1152

PESCE A, THIJS L, NARDINI M, DESMET F, SISINNI L, GOURLAY L, BOLLI A, COLETTA M, VAN DOORSLAER S, WAN X, ALAM M, ASCENZI P, MOENS L, BOLOGNESI M, DEWILDE S (2009). HisE11 and HisF8 provide bis-histidyl heme hexa-coordination in the globin domain of Geobacter sulfurreducens globin-coupled sensor. JOURNAL OF MOLECULAR BIOLOGY, vol. 386, p. 246-260

ASCENZI P, DI MASI A, COLETTA M, CIACCIO C, FANALI G, NICOLETTI FP, SMULEVICH G, FASANO M (2009). Ibuprofen impairs allosterically peroxynitrite isomerization by ferric human serum heme-albumin. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, p. 31006-31017

ASCENZI P, DI MASI A, DE SANCTIS G, COLETTA M, FASANO M (2009). Ibuprofen modulates allosterically NO dissociation from ferrous nitrosylated human serum heme-albumin by binding to three sites. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 387, p. 83-86

ASCENZI P, DE MARINIS E, DI MASI A, CIACCIO V, COLETTA M (2009). Peroxynitrite scavenging by ferryl sperm whale myoglobin and human hemoglobin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 390, p. 27-31

ASCENZI P, DE MARINIS E, VISCA P, CIACCIO C, COLETTA M (2009). Peroxynitrite detoxification by ferryl Mycobacterium leprae truncated hemoglobin O. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 380, p. 392-396

FANALI G, DE SANCTIS G, GIOIA M, COLETTA M, ASCENZI P, FASANO M (2009). Reversible two-step unfolding of heme-human serum albumin: a (1)H-NMR relaxometric and circular dichroism study. JBIC, vol. 14, p. 209-217

CIACCIO C, TUNDO GR, GRASSO G, SPOTO G, MARASCO D, RUVO M, GIOIA M, RIZZARELLI E, COLETTA M (2009). Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity. JOURNAL OF MOLECULAR BIOLOGY, vol. 385, p. 1556-1567

GIOIA M, MONACO S, VAN DEN STEEN PE, SBARDELLA D, GRASSO G, MARINI S, OVERALL CM, OPDENAKKER G, COLETTA M (2009). The collagen binding domain of gelatinase A modulates degradation of collagen IV by gelatinase B. JOURNAL OF MOLECULAR BIOLOGY, vol. 386, p. 419-434

ASCENZI P, IMPERI F, COLETTA M, FASANO M (2008). Abacavir and warfarin modulate allosterically kinetics of NO dissociation from ferrous nitrosylated human serum heme-albumin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 369, p. 686-691

NARDINI M, PESCE A, THIJS L, SAITO J.A, DEWILDE S, ALAM M, ASCENZI P, COLETTA M, CIACCIO C, MOENS L, BOLOGNESI M (2008). Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. EMBO REPORTS, vol. 9, p. 157-163

GEMMA S, CAMPIANI G, BUTINI S, KUKREJA G, COCCONE S.S, JOSHI, B.P, PERSICO, M, NACCI V, FIORINI I, NOVELLINO E, FATTORUSSO E, TAGLIATATELA-SCAFATI O, SAVINI L, TARAMELLI D, BASILICO N, PARAPINI S, MORACE G, YARDLEY V.F, CROFT S, COLETTA M, MARINI S, FATTORUSSO C (2008). Clotrimazole scaffold as an innovative pharmacophore towards potent antimalarial agents: design, synthesis, and biological and structure-activity relationship studies. JOURNAL OF MEDICINAL CHEMISTRY, vol. 51, p. 1278-1294

CIACCIO C, COLETTA A, DE SANCTIS G, MARINI S, COLETTA M (2008). Cooperativity and allostery in haemoglobin function. IUBMB LIFE, vol. 60, p. 112-123

BOLLI A, CIACCIO C, COLETTA M, NARDINI M, BOLOGNESI M, PESCE A, GUERTIN M, VISCA P, ASCENZI P (2008). Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide - a comparative study. THE FEBS JOURNAL, vol. 275, p. 633-645

ASCENZI P, DE MARINIS E, COLETTA M, VISCA P (2008). H2O2 and NO scavenging by Mycobacterium leprae truncated hemoglobin O. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 373, p. 197-201

LOMBARDI F, FASCIGLIONE GF, D'APICE MR, VIELLE A, D'ADAMO M, SBRACCIA P, MARINI S, BORGIANI P, COLETTA M, NOVELLI G (2008). Increased release and activity of matrix metalloproteinase-9 in patients with mandibuloacral dysplasia type A, a rare premature ageing syndrome. CLINICAL GENETICS, vol. 74, p. 374-383

SINIBALDI F, FIORUCCI L, PATRIARCA A, LAUCERI R, FERRI T, COLETTA M, SANTUCCI R (2008). Insights into cytochrome c-cardiolipin interaction. Role played by ionic strength. BIOCHEMISTRY, vol. 47, p. 6928-6935

LAUCERI R, FASCIGLIONE G.F, D'URSO A, MARINI S, PURRELLO R, COLETTA M (2008). Kinetic investigation of porphyrin interaction with chiral templates reveals unexpected features of the induction and self-propagation mechanism of chiral memory. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, p. 10476-10477

AURELI L, GIOIA M, CERBARA I, MONACO S, FASCIGLIONE GF, MARINI S, ASCENZI P, TOPAI A, COLETTA M (2008). Structural bases for substrate and inhibitor recognition by matrix metalloproteinases. CURRENT MEDICINAL CHEMISTRY, vol. 15, p. 2192-2222

DE SANCTIS, G, PETRELLA, G, CIACCIO, C, FEIS, A, SMULEVICH, G, COLETTA M (2007). A comparative study on axial coordination and ligand binding in ferric mini myoglobin and horse heart myoglobin. BIOPHYSICAL JOURNAL, vol. 93, p. 2135-2142

SALADINO R, FIANI C, CRESTINI C, ARGYROPOULOS DS, MARINI S, COLETTA M (2007). An efficient and stereoselective dearylation of asarinin and sesamin tetrahydrofuorofuran lignans to acuminatolide by methyltrioxorhenium/h2o2 and uhp systems. JOURNAL OF NATURAL PRODUCTS, vol. 70, p. 39-42

THIJS L, VINCK E, BOLLI A, TRANDAFIR, F, WAN X, HOOGEWIJS D, COLETTA M, FAGO A, WEBER R.E, VAN DOORSLAER S, ASCENZI P, ALAM M, MOENS L, DEWILDE S (2007). Characterization of a globin-coupled oxygen sensor with a gene-regulating function. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, p. 37325-37340

GIOIA M, MONACO S, FASCIGLIONE G.F, COLETTI A, MODESTI A, MARINI S, COLETTA M (2007). Characterization of the Mechanisms by which Gelatinase A, Neutrophil Collagenase, and Membrane-Type Metalloproteinase MMP-14 Recognize Collagen I and Enzymatically Process the Two alpha-Chains. JOURNAL OF MOLECULAR BIOLOGY, vol. 368, p. 1101-1113

ASCENZI, P, PETRELLA, G, COLETTA M (2007). Ferricyanide-mediated oxidation of ferrous nitrosylated sperm whale myoglobin involves the formation of the ferric nitrosylated intermediate. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 359, p. 871-876

ASCENZI P, BELLELLI A, COLETTA M, COLOSIMO A, FALCIONI G, GIACOMETTI GM, IPPOLITI R, ZOLLA L, GIARDINA B (2007). Multiple strategies for O2 transport: from simplicity to complexity. IUBMB LIFE, vol. 59, p. 1-17

MONACO S, GIOIA M, RODRIGUEZ J, FASCIGLIONE G.F, DI PIERRO D, LUPIDI G, KRIPPAHL L, MARINI S, COLETTA M (2007). Modulation of the proteolytic activity of matrix metalloproteinase-2 (gelatinase A) on fibrinogen. BIOCHEMICAL JOURNAL, vol. 402, p. 503-513

FEIS A, TOFANI L, DE SANCTIS G, COLETTA M, AND SMULEVICH G (2007). Multiphasic Kinetics of Myoglobin/SDS Complex Formation. BIOPHYSICAL JOURNAL, vol. 92, p. 4078-4087

ASCENZI P, CIACCIO C, COLETTA M (2007). Peroxynitrite-mediated oxidation of ferrous carbonylated myoglobin is limited by carbon monoxide dissociation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 363, p. 931-936

RODRIGUEZ, J, DI PIERRO, D, GIOIA, M, MONACO, S, DELGADO, R, COLETTA M, M, AND MARINI, S (2006). Effects of a natural extract from Mangifera indica L, and its active compound, mangiferin, on energy state and lipid peroxidation of red blood cells. BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1760, p. 1333-1342

MONACO, S, SPARANO, V, GIOIA, M, SBARDELLA, D, DI PIERRO, D, MARINI, S, COLETTA M, M (2006). Enzymatic processing of collagen IV by MMP-2 (gelatinase A) affects neutrophil migration and it is modulated by extracatalytic domains. PROTEIN SCIENCE, vol. 15, p. 2805-2815

SINIBALDI, F, PIRO, M.C, COLETTA M, M, AND SANTUCCI, R (2006). Salt-induced formation of the A-state of ferricytochrome c--effect of the anion charge on protein structure. THE FEBS JOURNAL, vol. 273, p. 5347-5357

CIACCIO, C, GAMBACURTA, A, DE SANCTIS, G, SPAGNOLO, D, SAKARIKOU, C, PETRELLA, G, AND COLETTA, COLETTA M (2006). rhEPO (recombinant human eosinophil peroxidase): expression in Pichia pastoris and biochemical characterization. BIOCHEMICAL JOURNAL, vol. 395, p. 295-301