Elena Zuriaga, Laura Molina, María Luisa Badenes and Carlos Romero*

Physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the Rosaceae

Elena Zuriaga, Laura Molina, María Luisa Badenes and Carlos Romero*

Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113 Moncada, Valencia, Spain.

Carlos Romero* (corresponding author)

Phone number: +34 96 342 40 00; Fax number: +34 96 342 40 01

SUPPLEMENTAL TABLES

Supplemental Table 1.- SSR markers tested for the PPM screening on the whole ‘Canino’ genome.

Acronyme / Species /

Number of SSR

/

Reference

BPPCT / P. persica / 20 (7)a / Dirlewanger et al. (2002)
Gol / P. armeniaca / 2 (2) / Vera-Ruiz et al. (2010)
CPDCT / P. dulcis / 1 (1) / Mnejja et al. (2005)
CPPCT / P. persica / 10 (2) / Aranzana et al. (2002)
CPSCT / P. salicina / 21 (10) / Mnejja et al. (2004)
EPPCU / P. persica / 5 (2) / Howad et al. (2005)
M/MA / P. persica / 14 (3) / Yamamoto et al. (2002)
pchcms/pchgms / P. persica / 6 (2) / Sosinski et al. (2000), Verde et al. (2004),
Verde et al. (2005) and Wang et al. (2002)
ssrPaCITA / P. armeniaca / 16 (6) / Lopes et al. (2002)
UDAp / P. armeniaca / 21 (6) / Messina et al. (2004)
UDP / P. persica / 16 (4) / Testolin et al. (2000) and Cipriani et al. (1999)
PGS / P. persica / 48 (8) / This work
TOTAL / 180 (53)

a Number of SSRs polymorphic in ‘Canino’ is indicated between brackets.

Supplemental Table 2.- SSR primers developed from the peach genomic sequence corresponding to the scaffolds _2, _4, _5, _6, _7 and _8. Primer sequence, position, repeat motif, peach fragment size as well as SSR alleles amplified in apricot cvs. ‘Goldrich’ and ‘Canino’ are indicated.

Name / F/R / Primer sequence / Repeat motif / Start on scaffold (Mb) / Peach
size (bp) / ‘Goldrich’
alleles / ‘Canino’
alleles
PGS2.01 / F / GAAGTCCAGTGGCAAAATCG / (TC)19 / 6,27 / 203 / N.A.a / N.A.
R / AGCCGGTTCTTTTGACACC
PGS2.02 / F / AAGGGGAAGGAGAAGCAAAG / (TAA)10 / 6,28 / 204 / N.A. / N.A.
R / GGAACAACTTCTCCCGAACA
PGS2.03 / F / GAAAATCAGTGGCACAATCG / (TC)44 / 4,36 / 238 / 177 / 177
R / TTTGGCACCGGTCTCATC
PGS4.01 / F / GCTTTGTGGAAGACGTTGGT / (TC)35 / 3,46 / 341 / 348 / 356
R / GGGCTCTAAAGCCAGGCATA
PGS4.02 / F / CCCCTTCCACATACCTCAAA / (CA)35 / 3,49 / 191 / 169/175 / 169
R / AAACACTGGGATGCACCTCT
PGS4.03 / F / GCCTGGATGACGTTGTGATA / (AG)25 / 3,55 / 230 / N.A. / N.A.
R / ATTGATACCACGGCTGACCT
PGS4.04 / F / GAGCCCCTAATGTTCATTGC / (TC)31 / 11,99 / 250 / 278 / 265
R / AGCATTCAAACCCAAACTCG
PGS4.05 / F / TCAATTCCAGCCAGAAATCC / (TC)22 / 12,03 / 194 / 171 / 171
R / TGGGAAAAGTTGGTGACCTT
PGS4.06 / F / GCCTTGTTTTTAGCCCAGAA / (AT)15 / 12,11 / 216 / N.A. / N.A.
R / TTGATCCCATCACCGAAAGT
PGS4.07 / F / CTGCATAAAGCCATTGCAGA / (GA)7(GA)9 / 18,14 / 178 / 179 / 176
R / CATTGCTGGCTTTTCTCCTC
PGS4.08 / F / TTAAGCTCCATGGCCAAAAC / (GA)30 / 18,22 / 209 / N.A. / 204
R / TGCAACAAGTCCAAAAAGCA
PGS5.01 / F / TGAGAGCTTGTGATGTCTGTG / (AG)21 / 0,43 / 231 / 227 / 197
R / GACTAAGCATTCGTCAAGCTG
PGS5.02 / F / CGCTCTTAAGCACGTTGTCA / (AG)29 / 0,48 / 187 / 178/183 / 183
R / AACCTTCCCGTACGTTCCTT
PGS5.03 / F / GGTGGTGTCATTGTGTTGTG / (GA)36 / 5,06 / 176 / 138 / 136/138
R / CAACTTGGGTTGAAGGAGGA
PGS5.04 / F / CTCCCAGTTGCTCTCACCAT / (CT)23 / 5,15 / 244 / 153/null / 157/null
R / AGGAGCATCGCAAATCAGAC
PGS5.05 / F / ATGACGCAAGGTGGTGTGT / (GT)35 / 5,19 / 299 / MLb / ML
R / CGTTCAACAATGATGGTTCC
PGS5.06 / F / AGAATTGGTGCCATGGTTGT / (GA)18 / 15,09 / 485 / 218 / 218
R / CTCGTTTGGTATGCCCATTT
PGS5.07 / F / CATGCATGGATCCAAAACC / (AG)28 / 15,24 / 233 / ML / ML
R / GTTGGCTGGCTCTTGTTAATC
PGS5.08 / F / CCTCTTGCTGCAAAACATCA / (GA)28 / 15,30 / 247 / 219 / 219
R / TTTTCTCCCTCTGCATGACC
PGS6.01 / F / TCCTACACTGCCACACAACA / (GA)24 / 0,12 / 277 / 284/289 / 289
R / GAAAGCGAGCTCCAGGATAA
PGS6.02 / F / AAATGGGACCCTCACCAACT / (TC)21 / 1,14 / 244 / 265/269 / 263
R / CTCACAAGCTGTTCAATGCTG
PGS6.03 / F / TTGAGCCCTAGCTCTTGCTT / (TC)15 / 1,23 / 211 / 221/225 / 227
R / CTCGCAGCAATTTCCAAGTC
PGS6.04 / F / AATGACGGGTGTGTGGCTAT / (CT)17 / 4,95 / 161 / 149/158 / 149/155
R / CCAAATGGCCGCAGTAGTAG
PGS6.05 / F / CGCATGAATTTTGATCCAC / (GA)23 / 5,17 / 225 / N.A. / N.A.
R / ACCCGCATTTCTCAACATCT
PGS6.06 / F / TAGATTATGGACGGGGCAAA / (CT)47 / 5,25 / 299 / N.A. / N.A.
R / CCAAAAGCAAGCATGATGTC
PGS6.07 / F / GTGGTGGATGATTGACCATT / (TG)18(GA)21 / 9,33 / 190 / 183/185 / 185/201
R / TGGCAGAGCTTTGTCAGCTT
PGS6.08 / F / GCCAACAAGGAACAAACCAT / (AT)21 / 9,35 / 213 / 236/238 / 238
R / GCCCATTTTTGCTTAAGGTC
PGS6.09 / F / TGATCAGGAATCTGTCACCTCA / (AG)18 / 9,34 / 214 / 202 / N.A.
R / CCTATAAACCACCCACTGAAGC
PGS7.01 / F / CTGTCGCGAGAAGCTCTAGG / (TC)50 / 1,01 / 162 / N.A. / 101
R / GTGACCGAAGACCACTGCTT
PGS7.02 / F / CCACGTGGACAATGAGAATG / (CT)22 / 1,06 / 398 / N.A. / N.A.
R / TAGAGCCATGTGTGGAGTCG
PGS7.03 / F / TATGTGGAGATGGGGAGGAA / (GA)25 / 13,07 / 219 / N.A. / N.A.
R / CCACCATCCCCTCCATAAT
PGS7.04 / F / CGGTGCCCTTTATAACCAAT / (CT)25 / 13,13 / 204 / N.A. / N.A.
R / GAGAGTTGGACTCCCCTCAA
PGS7.05 / F / GATGACGTGGCATGAACAAG / (TC)27 / 13,08 / 213 / 217/221 / 205/215
R / CCCCAGACTTGTCAAAGAGC
PGS7.06 / F / GAGGCATCAAAGGCCAATAC / (AG)23 / 18,90 / 245 / N.A. / N.A.
R / TTTGGAGCCGTTACACCTTC
PGS7.07 / F / GGGTGGGCACATACTAATTCA / (TG)21(AG)28 / 19,10 / 236 / N.A. / 218
R / TGCTTTTCTGGAAACTTCTGG
PGS7.08 / F / ACAGCACAACCAATCAGCTT / (TC)27 / 19,21 / 394 / N.A. / N.A.
R / TGACCACTTTTGACGGTCTG
PGS8.01 / F / GGCTCGAATCGGAGTTGTAA / (TC)25 / 3,28 / 206 / ML / ML
R / CTATGGTGGGTCTTGGAAGC
PGS8.02 / F / GTCTCGCAAAAATGGGGTTA / (GA)25 / 3,92 / 237 / 326/343 / 330
R / GTAATCAGCACAGCGTTCCA
PGS8.03 / F / GTGGGGTTTCATCACTGGAG / (GA)31 / 3,97 / 245 / N.A. / N.A.
R / AATAGGACACCCCCTTGACA
PGS8.04 / F / CGCCTTCTCCATCATCTCTT / (TC)28 / 7,35 / 282 / N.A. / N.A.
R / TGCGAATTAATGGCATGGTA
PGS8.05 / F / GGCCATGACTTGGTCAATCT / (GA)29 / 7,39 / 239 / 251 / 243/251
R / GGGGGAAGTTGGAGGTTACA
PGS8.06 / F / CCACTACATGGGCACACTTG / (AG)20 / 7,51 / 173 / ML / ML
R / CAAAGGCAAAAAGTGCTGGT
PGS8.07 / F / CTGAAGCAAACAGGAACATGA / (GT)30 / 11,22 / 237 / ML / ML
R / AGGTTGCCTCAAGAAGTGTGA
PGS8.08 / F / CTGCCACAATCACTTTGAGC / (TC)38 / 11,30 / 477 / N.A. / N.A.
R / TAATGATGGCGGTGAGACAA
PGS8.09 / F / GAGCTCTGGCAATGAAGTCC / (CT)23 / 11,41 / 171 / 180 / 180
R / AGCCACAAATCCCTCACTTG
PGS8.10 / F / CCAAGTGGTTCCATTTTTGG / (AG)23 / 18,36 / 224 / N.A. / 220
R / GAACCCGGCAAACCTAATTT
PGS8.11 / F / TTCCTGCCACAGCCTCTTAT / (GAA)14 / 18,42 / 171 / N.A. / N.A.
R / GCAATAACGGCCACCATATC
PGS8.12 / F / GCATTTCGGTTGGAAGAAGA / (GA)42 / 18,47 / 400 / N.A. / N.A.
R / TTCGTGATGTTTGTGGCTTT

a N.A. Not amplified

b ML. Multi-loci pattern

Supplemental Table 3.- SSR primers developed from the peach genomic sequence corresponding to the scaffold _3. Primer sequence and position, repeat motif and peach fragment size, and SSR alleles amplified in apricot cvs. ‘Goldrich’ and ‘Canino’ are indicated.

Name / F/R / Primer sequence / Repeat motif / Start on scaffold_3 (Mb) / Peach
size (bp) / Goldrich
alleles / Canino
alleles
PGS3.01 / F / AACGTGATAGCCAAGCCTGT / (GA)30 / 16,21 / 175 / 176 / 168/176
R / TGGTCACCTTCCTTCATATCC
PGS3.02 / F / TGCTGCAAATCCTAAAGGAGA / (TC)27 / 16,30 / 175 / 163/174 / 166/174
R / AAAGGAGGGACCTCATTGAAA
PGS3.03 / F / ATGTACCCGGCTATGCAAAC / (TC)20 / 16,41 / 182 / 162/178 / 156/162
R / AAAGCTCGGGACTTTCGATT
PGS3.04 / F / GAGCTCACATGAGAAGCATGA / (CT)61 / 16,52 / 198 / N.A.a / N.A.
R / CCCCAACCCAACACTCACTA
PGS3.05 / F / TCATCCAACTCCCCATTGAT / (AT)20 / 16,64 / 198 / 171 / 171
R / TCTCCTCGTACCGTCGAGTT
PGS3.06 / F / TAAGCAATGCAAACTGGAAA / (AG)22 / 16,70 / 164 / 152/160 / 152
R / CAAGTGAATGAAGTGCCAGA
PGS3.07 / F / TGGAGATCTCAAGGATCCAG / (TA)23 / 16,84 / 184 / 141 / 141
R / AAGCAATCTCACGTGTCCTC
PGS3.08 / F / ATTGAATGTGACCACGGAAG / (TC)22 / 16,91 / 152 / 157 / 157
R / GGAACTTCAGAGTGGCAAAC
PGS3.09 / F / AAACTCGCATTCAATTCCAA / (GA)28 / 17,01 / 221 / 204 / 204
R / CAATGACACGTGTGTCTGCT
PGS3.10 / F / CCCTGTTTCAGTCTCATCGT / (GT)25(GA)18 / 17,16 / 194 / 130 / 129/130
R / ACTTTGAGAAGTGAAGGTACGC
PGS3.11 / F / AACCTCTCCAAGGCCACTAT / (CT)23 / 17,22 / 175 / N.A. / 156
R / CGGCTGGAGCCTTATGTAAA
PGS3.12 / F / CTGATGGAAGCACATGATGA / (CT)24 / 17,38 / 142 / 162 / 148/162
R / CTGTCTCCGCCATGAATTT
PGS3.13 / F / TTTGCAGCCTTGAAATTTTAGT / (TA)21 / 17,54 / 222 / 229 / 202/229
R / ATAGCTCATCCACCGTTCAA
PGS3.14 / F / ACCACAAGACATACGCAGTG / (GA)48 / 17,63 / 199 / N.A. / N.A.
R / GCTGTCAAAGCTGATTGGTT
PGS3.15 / F / AGTCTTTCGTAGGCCCATTT / (CT)31 / 17,71 / 297 / 266/267 / 267/274
R / ATTTTCGCTCACCCTGAATC
PGS3.16 / F / CAAGATTCTTCTCACACCCAAA / (CT)25 / 17,80 / 249 / N.A. / N.A.
R / TTTAGGTCACGGATCACACC
PGS3.17 / F / AAGCCCAAGGTAAAACCCTA / (AG)24 / 17,98 / 207 / N.A. / N.A.
R / ACCTTGCAAGAATAGGCACA
PGS3.18 / F / AGCAAGCAAGCATCTTCAAC / (TC)33 / 18,06 / 183 / 147 / 147
R / TGTGCATCATCACTGACATTT
PGS3.19 / F / GCACACATGCACAGAGAGAC / (AG)32 / 18,12 / 166 / 147 / 199
R / ATACTGCCATCCAGAAATGC
PGS3.20 / F / TGTGGCAGCAATGGATAGAT / (TC)39 / 18,25 / 148 / 148 / N.A.
R / ACTCGTCAAGCCCGTCAA
PGS3.21 / F / TCAGATCTCAAGGGATTGGT / (TC)23 / 18,40 / 224 / 229/244 / 244
R / GCACCATAGATGCCAAATCT
PGS3.22 / F / TCTGATTGCAGGTAAGGACAG / (CT)25 / 18,49 / 299 / 306/310 / 306
R / TATCTTGATATCGGCCTGGA
PGS3.23 / F / TGACTTTCTGCATCTTGACCT / (AG)24 / 18,61 / 170 / 179/188 / 188
R / CTTTGCTTCCGTTAATCCAA
PGS3.24 / F / CCAAAAGCTTGACTTGGAAA / (TA)15 / 18,77 / 198 / MLb / ML
R / GGTGTTTGTTTGATGCTTGG
PGS3.25 / F / CAGCTGTGTGGAGAATGAGA / (AG)17 / 18,87 / 163 / 180/192 / 186/192
R / GTATGCGCCACTGCTTTATC
PGS3.26 / F / GACTTTTGCTACAGCCAGGA / (AG)19 / 18,94 / 150 / 155/166 / 160/166
R / ATTCCAACCCCACCTAAACT
PGS3.27 / F / CCTTGCCATTTTAATACCTGAG / (AG)28 / 19,03 / 181 / N.A. / N.A.
R / AGGCCATTTGCCTAGCTAAC
PGS3.28 / F / ACAACGCATCAAGAATTGGT / (TA)18 / 19,14 / 133 / 141 / 141
R / ATGATGTATGGGCTTGAAGG
PGS3.29 / F / TGCCCTAGAATTTGCTTCAG / (TG)20 / 19,25 / 166 / 159 / 159
R / ATCACCATTCATCAGCAACC
PGS3.30 / F / CCACTCCACCGTATCTCTTTT / (CT)21 / 19,34 / 186 / 247 / 242
R / GGTGAAGAGGGTGGAGAGAA
PGS3.31 / F / ATTTTGACTTTTCCTGATTTGG / (CT)22 / 19,45 / 182 / ML / ML
R / TGTTTGGTTTGCACAGATTG
PGS3.32 / F / TCAAATGCCATTGAAGGTAAA / (AG)28 / 19,60 / 249 / 256 / 256
R / CAGGTTCTTCGTCCAAGTGT
PGS3.33 / F / AAATGTGAGCTCCTCAACCA / (GA)36 / 19,66 / 166 / 133/139 / 133/139
R / AAGAGAAATGCACCGACTGT
PGS3.34 / F / CTCGTAGAAATTGGATGAGCAG / (GA)29 / 17,75 / 197 / 185/191 / 185/193
R / TCCTTCTTTGGTTCCACACC
PGS3.35 / F / GAAATCGACCTTGTCGTCCT / (AG)20 / 17,85 / 197 / N.A. / N.A.
R / CGCAGTCGCTACTCTCTGTG
PGS3.36 / F / TGGAAAGTGGGAATTTGTTTG / (AT)17 / 17,95 / 237 / 148/162 / 325/327
R / TCCGCCCAAATATTTAGACTTC
PGS3.37 / F / AATTGTTACGTTTTGCCTTCAA / (TC)17(CA)16 / 18,00 / 227 / 189/202 / 189
R / AACACAATGGTTCTTGCACA
PGS3.38 / F / CAACCCCTCCTCTCACATTT / (GA)22 / 18,05 / 248 / 246/264 / 264
R / GGGTTTTTGGGTGTTCTAGC
PGS3.39 / F / GCTAGCTGTGAATGGCTGTG / (AG)20 / 18,07 / 177 / 165 / 165
R / CCACTTCAAATCATCCCTCCT
PGS3.40 / F / GGACCCGAAATTAGGGATTT / (GA)21 / 18,14 / 181 / 128 / 177
R / CCGCAGCGCATTCAATTAT
PGS3.41 / F / TGCACAAGGGTTAAGGTACG / (CA)21 / 18,18 / 222 / N.A. / N.A.
R / GGCTTCCAATCGTGTCTTGT
PGS3.42 / F / TTCTTTCCGAACTCCCTTCA / (CT)24 / 18,22 / 209 / 206/224 / 206
R / TACAACCCACCAAAGGGAAC
PGS3.43 / F / CCCAGTGGCACAATCGTAA / (TC)36 / 18,24 / 264 / N.A. / N.A.
R / AAAACCGCCGCTGTGAAC
PGS3.44 / F / TCTTGTGTTATCCGTCCGTTT / (CT)33 / 18,29 / 319 / 295/307 / 307/309
R / TGCTAAGGAGCCCTTGAATG
PGS3.45 / F / ATAACCTGAGTGGCCCTGTG / (TC)22(CA)21 / 18,33 / 225 / 237 / N.A.
R / CCGTTGCACATATCAGAGGA
PGS3.46 / F / GTCACACGCATGTGTCCAAT / (GA)26 / 18,47 / 175 / 163/173 / 163
R / TTGCAGGTCTGTTCTTGTGC
PGS3.47 / F / TGTGGCCCAATTTCTTTCTT / (CT)19 / 18,52 / 211 / 242/246 / 242
R / CTGGGCTTCTCTGTGACCTC
PGS3.48 / F / ACCATCTTCCATCCCTCCTC / (AG)18 / 18,60 / 239 / 256/264 / 264
R / CACAACTCATCCACCCTCCT
PGS3.49 / F / TCCCAAACCAACTTAATGGA / (AAT)16 / 18,63 / 151 / N.A. / N.A.
R / CCAAATGCTCATCCCTCTTT
PGS3.50 / F / AGAGGACCGTAAGGGTGTCC / (TA)16 / 18,83 / 179 / N.A. / N.A.
R / CCACTTGCTTTTGCCATCTT
PGS3.51 / F / GCTAGAGGGTGCTGCAAATC / (AG)18 / 17,83 / 189 / ML / ML
R / TACTTGGCATGGGCAAAAAT
PGS3.52 / F / CATGTGGTTCTCCTGCAACTT / (TA)17 / 17,96 / 199 / 194/208 / 194
R / ATGCATGTGCAAGCCAATAA
PGS3.53 / F / CTGACAACAGCATGCACCTT / (GA)17 / 18,07 / 193 / 199/203 / 199
R / TAACATCACCGCCGTCACT
PGS3.54 / F / CTTTCGTCTGGGCTGATTGT / (TC)29 / 18,12 / 247 / 280/282 / 282
R / ATGGAGGCAATGGCTATGAG
PGS3.55 / F / GCTGTTGTTGGGTTGTGTGT / (AT)17 / 18,19 / 173 / 449 / 449
R / CCAAAGCCATAACCTTCAGC
PGS3.56 / F / CGTCCAATTAGAAGACGCCATA / (CT)19 / 18,22 / 299 / 251/258 / 258
R / CAAGTAGGGCAACGACAACA
PGS3.57 / F / GAAGGACAAAAGGCACCAAC / (TC)31 / 18,23 / 244 / N.A. / N.A.
R / TGCAAGAATCGAAGCATAGG
PGS3.58 / F / CGTAACTCATGAGGGTCCAAA / (TA)18 / 18,24 / 159 / 95/99 / 95
R / CTACAAGGGGTGTCCCTCAA
PGS3.59 / F / TGTTGTGTGCATCTCACTCG / (CT)22 / 18,50 / 193 / 211/215 / 215
R / CCATCTTTCCCATCCTTTCA
PGS3.60 / F / GACCTCCCGCATTTCACTAC / (CT)21 / 18,51 / 237 / 293/317 / 317
R / CGTCTTCAAATTCATCGTGGT
PGS3.61 / F / TTACTTCTTTTGGGGCAAGC / (TC)19 / 18,58 / 150 / 173 / 173
R / ATGTTCGCCTTCTGGATCTG
PGS3.62 / F / AGCTTCCTCTATTCTTGGTGGT / (CT)22 / 18,61 / 286 / 336/350 / 348/350
R / GCTTTTCCCCGAGCTAATTC
PGS3.63 / F / CCGACAAAAGGAAATTGCTC / (CT)15 / 18,65 / 182 / 218/220 / 220
R / CACCAAATGGACTGCACCTT
PGS3.64 / F / CCACCACTTGCACCACTACA / (CCA)16 / 18,70 / 196 / ML / ML
R / ATTTTTGTGCGTCTCCCAAT
PGS3.65 / F / CCATACCTCAATTCCAAAGC / (TC)27 / 18,31 / 185 / ML / ML
R / TTGTTGTTGCCGACTTATGC
PGS3.66 / F / GGCACGTATGCATGTGATGT / (TA)12 / 18,34 / 316 / 322/328 / 328
R / CGGCACGCTTTCATTACTTC
PGS3.67 / F / GCTGCACTTTTGCTCACCTT / (AT)9 / 18,35 / 193 / 211/217 / 211
R / TGACTTCCACCAAAAGCTCA
PGS3.68 / F / CGACGACACGAGTTCATAGA / (CT)9 / 18,37 / 300 / 311/338 / 338
R / CTCCAGTTTCCGGTGAGCTT
PGS3.69 / F / CCAAGAAAGTTGTCCTTCTGC / (TC)22 / 18,38 / 197 / 202/227 / 202
R / TGCTGCATCCTTCTATGCTG
PGS3.70 / F / TGGTGGGAATGGATATAAGG / (CT)18 / 18,40 / 194 / 200/201 / 200
R / CCACTCAACTTGACCCAACC
PGS3.71 / F / ACCACCCCCTATCCCTATTG / (CT)13 / 18,40 / 220 / 255/259 / 259/261
R / ACTTGCAAACCCCCTTGATT
PGS3.72 / F / GCATCAGCAGTTACAGAAGCA / (GGA)8 / 18,41 / 315 / ML / ML
R / GCGAAATTGGGTTGGAGATA
PGS3.73 / F / TGCGAGGACTTCTTCTGGAC / (GAA)8 / 18,43 / 350 / 368 / 368
R / ACACCCAACTTCACCAATGA
PGS3.74 / F / CCTCGTCAATTGCCAACTCT / (TTC)8 / 18,46 / 250 / 262/265 / 265
R / ATTGGTTATCGGCATTCTCG
PGS3.75 / F / TGGTTAGGGCTCATCTTGTT / (TTC)8 / 18,48 / 341 / N.A. / N.A.
R / AATTCGGGTTGTTGCTCAAG
PGS3.76 / F / GCTGAATTTGCTGCTAGTGC / (TG)14 / 18,49 / 181 / 192 / 192
R / ACATGTACCCCAAACCGAAA
PGS3.77 / F / AGGCGAACCATTGGTGAAAG / (CT)22 / 18,50 / 272 / 273/276 / 273
R / AAGTCTTGCCCAGCAGAAAC
PGS3.78 / F / ACGATCGGGTGAAAGACAAG / (GAA)7 / 18,54 / 158 / 161/164 / 164
R / TCTCGACCGCCTTAAAACAT
PGS3.79 / F / AGAAGCTTGTGGCATGGAAG / (AG)14 / 18,57 / 180 / 460 / 460
R / CCACTGGAACAGTTGCAAGA
PGS3.80 / F / CTTTGTCCACCCACCAAGAT / (TC)8 / 18,59 / 456 / 474 / 474
R / GAAGGTCAAGTCAGTCCATTCA
PGS3.81 / F / CCCTGATTTTATCCCAAGCA / (AT)11 / 18,61 / 202 / 227 / 227
R / GCATTTGCAGAACCAACTGA
PGS3.82 / F / TCTTGTTCTCGATCCGCTTC / (CT)8 / 18,61 / 152 / 148 / 148
R / TCCCTCACTTCCCTCTTTCC
PGS3.83 / F / TGAGGTGGCATAGGCATTCT / (TC)11 / 18,61 / 247 / ML / ML
R / CTCAAACCACCACCACTCAA
PGS3.84 / F / GGGAGGGAAAAGGAGAGAAG / (TG)13(AG)10 / 18,61 / 247 / N.A. / N.A.
R / CTGACCCCTAACCCCTCATT
PGS3.85 / F / TGGTACAAGCTGATCCTCCT / (AG)12 / 18,62 / 196 / ML / ML
R / GCCAAAACTCAGGAGAGAGA
PGS3.86 / F / GCTCGCTTGCCGTTATGTTA / (CT)13 / 18,63 / 168 / 170/179 / 170
R / TAGGCCGTTGATGGTTTCTC
PGS3.87 / F / TGGGGCCAAAATGAAGATAG / (GAA)7 / 18,63 / 329 / 335/340 / 340
R / TCTGCTCTCTGCTATGTGGAA
PGS3.88 / F / TCACATGCCCCTCAACAATA / (GA)12 / 18,65 / 248 / 278 / N.A.
R / TTCCCCTTTGAAAGTCCTCA
PGS3.89 / F / ACTGCCATGTTACGCAATGA / (CT)10 / 18,66 / 403 / 404/405 / 405
R / GGGCATGTTGTGGATTTGTT
PGS3.90 / F / CGTTGGTTCATTACCAAGCA / (GA)8 / 18,69 / 162 / ML / ML
R / CGTTTTACGCCATCCTCATT
PGS3.91 / F / GGTTGAGCAATGGTGCTTTT / (TA)12 / 18,70 / 102 / 285/null / N.A.
R / TCCGCCTGGCTATACTGTTT
PGS3.92 / F / ACCACCTCCACTTCCACATC / (CCA)7 / 18,70 / 163 / ML / ML
R / AGTCTCCTGGAAGCATGGTG
PGS3.93 / F / CACCTCTCTTGCAGTCTTTGA / (GTTT)4 / 18,73 / 229 / 206/207 / 206
R / CTTCTCCCTTGGGGAAAATC
PGS3.94 / F / CAACGAACAGAATGTTGCAG / (GGT)7 / 18,74 / 288 / N.A. / N.A.
R / ATGACACCACCTCCACTTCC
PGS3.95 / F / AGAGTCCACACTGCAAAAGC / (CAT)9 / 18,75 / 343 / 344 / 344
R / GGACACTGATACGTGCGGTA
PGS3.96 / F / TGGCCACAATTAATGGGAGA / (CT)14 / 18,76 / 427 / 434/441 / 441/442
R / TCGGAGAACTTCTTGTGCAT
PGS3.97 / F / GGCTAAACATGGCTGGTTGT / (GA)14 / 18,80 / 227 / N.A. / N.A.
R / CGTGCCAAGATGAGTTCGTA
PGS3.98 / F / GATCCGCCCCCTTATAACA / (TA)6 / 18,81 / 307 / N.A. / N.A.
R / GGATCCCTCAATGGAAGCTC
PGS3.99 / F / GATGATCATACGTCCCTACCC / (CA)11 / 18,81 / 201 / N.A. / N.A.
R / CCTTCAACCTTTTGCTTCACC
PGS3.100 / F / GAGAACATGGAGCGGAGAAG / (GA)9 / 18,84 / 223 / 239 / 239
R / TCATTAAGCCTCGGCTAGGG
PGS3.101 / F / AAAGCATCAGATCGTGACCA / (GA)9 / 18,84 / 207 / N.A. / N.A.
R / TCACCTCCCTGTTTCTTTGG
PGS3.102 / F / ATTGATCGGTGAAACGTGGT / (CT)8 / 18,85 / 241 / 251 / 251
R / CGACGTCGAATTCATTATGC

a N.A. Not amplified

b ML. Multi-loci pattern

Supplemental Table 4.- BACs anchored onto the apricot ‘Canino’ genetic map. Number of hybridisation positive BACs confirmed by PCR and BES obtained from each pool are also indicated.

Pool / Overgo probes1 / No of hybridization
positive BACs / No of anchored BACs
confirmed by PCR2 / No of BES
1 / OV.PGS3_22; _48; _61; _62; _63; _73; _78; _86; and _95 / 30 / 3 (108J24, 148H054 and 148J084) / 6
2 / OV.PGS3_443; _61; _63; _64; _703; _81; _84; _95; _1023 and OV.27C06.SP6 / 24 / 7 (215E14, 224A3, 234O11, 12J14, 217G17, 210A19 and 198D134) / 14
3 / OV.PGS3_63; _64; _84; _95; _983 and OV.108J24.SP6 / 38 / 10 (209M03, 148M17, 253J12, 170A18, 200J16, 13N08, 7M17, 59M14, 164D09 and 65P22) / 20
4 / OV.PGS3_24; _64; _72; _91; _93; _95; and OV.13N08.SP6 / 74 / 6 (251L05, 155M12, 160J21, 159P08, 95D02 and 161F24) / 12
Total / 4 pools with
22 distinct overgo probes / 166 / 26 / 52

1 Overgo probes were named according to linked SSRs previously identified from the peach genome or to the BES from which they were developed

2 PCRs were performed with SSRs linked to overgo probes. Non-redundant positive clones are indicated between brackets.

3Overgo probes developed upstream (OV.PGS3_44 and _70) or downstream (OV.PGS3_98 and _102) the M-locus region

4 BACs found entirely beyond the M-locus region were discarded

Supplemental Table 5. - Results of similarity searches between apricot BES and peach genome v1.0, apple genome v1.0 and strawberry genome v1.0 sequence using BLASTN. Query and hit records, percent id and P-value are indicated.

Peach / Malus / Fragaria
BAC clone / Query / Query_length / Hit / Length / Percent_id / P_value / Query_start / Query_end / Sbjct_start / Sbjct_end / Hit / Length / Percent_id / P_value / Query_start / Query_end / Sbjct_start / Sbjct_end / Hit / Length / Percent_id / P_value / Query_start / Query_end / Sbjct_start / Sbjct_end
148H05 / 148H05SP6 / 826 / scaffold_3 / 168 / 92,86 / 8,E-59 / 27 / 194 / 17974660 / 17974826 / MDC035286.10 - chr8 / 327 / 84,10 / 3,E-55 / 495 / 820 / 16423684 / 16423359 / LG6 / 249 / 79,12 / 4,E-14 / 513 / 761 / 19982134 / 19982382
148H05SP6 / 826 / scaffold_3 / 50 / 90,00 / 2,E-07 / 304 / 353 / 17974596 / 17974645 / MDC021515.56 - chr12 / 327 / 83,79 / 3,E-55 / 495 / 820 / 28531109 / 28531434 / LG6 / 211 / 78,67 / 4,E-08 / 558 / 768 / 24588535 / 24588745
148H05SP6 / 826 / scaffold_3 / 78 / 91,03 / 2,E-19 / 417 / 494 / 17974445 / 17974522
148H05T7 / 788 / scaffold_3 / 455 / 93,63 / 0,E+00 / 12 / 466 / 17886449 / 17886903 / MDC007266.317 - chr9 / 410 / 90,98 / 1,E-150 / 12 / 421 / 18380700 / 18380291
148H05T7 / 788 / MDC007266.317 - chr9 / 412 / 90,29 / 1,E-139 / 12 / 423 / 18380009 / 18379598
148H05T7 / 788 / MDC007266.317 - chr9 / 467 / 89,72 / 1,E-162 / 12 / 478 / 18381046 / 18380581
148H05T7 / 788 / MDC002735.283 - chr1 / 456 / 90,79 / 1,E-162 / 12 / 431 / 22362521 / 22362103
215E14 / 215E14T7 / 916 / scaffold_3 / 913 / 95,84 / 0,E+00 / 4 / 916 / 18424090 / 18425001 / MDC019370.84 - chr9 / 325 / 90,46 / 1,E-106 / 351 / 675 / 4715159 / 4715482 / LG6 / 78 / 94,87 / 1,E-26 / 392 / 469 / 31656304 / 31656381
215E14T7 / 916 / MDC009641.211 - chr9 / 334 / 89,52 / 1,E-100 / 351 / 683 / 4841521 / 4841853 / LG6 / 90 / 91,11 / 3,E-24 / 538 / 627 / 31656449 / 31656538
215E14T7 / 916 / MDC013527.426 -chr17 / 333 / 89,79 / 1,E-106 / 351 / 683 / 5307695 / 5308026
215E14SP6 / 885 / scaffold_3 / 639 / 92,96 / 0,E+00 / 14 / 651 / 18380006 / 18380642 / MDC010787.146 - chr17 / 78 / 85,90 / 2,E-07 / 658 / 733 / 5355219 / 5355296
209M03 / 209M03T7 / 845 / scaffold_3 / 293 / 82,94 / 3,E-49 / 38 / 319 / 18448218 / 18448500
209M03SP6 / 909 / scaffold_3 / 181 / 87,85 / 3,E-49 / 234 / 402 / 18413527 / 18413707 / MDC000744.74 - chr9 / 42 / 92,86 / 7,E-07 / 159 / 200 / 4739930 / 4739971
209M03SP6 / 909 / scaffold_3 / 68 / 95,59 / 5,E-23 / 440 / 507 / 18413709 / 18413776 / MDC013527.401 - chr17 / 42 / 90,48 / 1,E-05 / 159 / 200 / 5321481 / 5321522
209M03SP6 / 909 / MDC010787.88 - chr17 / 42 / 90,48 / 1,E-05 / 159 / 200 / 5324648 / 5324689
209M03SP6 / 909 / MDC010787.146 - chr17 / 42 / 90,48 / 1,E-05 / 159 / 200 / 5327180 / 5327221
108J24 / 108J24SP6 / 834 / scaffold_3 / 830 / 93,61 / 0,E+00 / 14 / 834 / 18444699 / 18445521 / MDC000117.336 - chr17 / 161 / 87,00 / 6,E-35 / 630 / 790 / 5276960 / 5277119 / LG6 / 114 / 86,84 / 7,E-22 / 721 / 834 / 31666987 / 31667100
108J24T7 / 840 / scaffold_3 / 836 / 94,26 / 0,E+00 / 6 / 840 / 18513054 / 18513889 / MDC021594.239 - chr9 / 107 / 89,00 / 2,E-19 / 207 / 310 / 4632534 / 4632640 / LG6 / 58 / 84,48 / 9,E-03 / 242 / 299 / 31781307 / 31781364
108J24T7 / 840 / MDC021594.239 - chr9 / 71 / 85,00 / 1,E-08 / 441 / 511 / 4632784 / 4632854 / LG6 / 69 / 85,51 / 6,E-07 / 409 / 477 / 31781144 / 31781212
108J24T7 / 840 / MDC021594.239 - chr9 / 145 / 78,00 / 1,E-02 / 692 / 836 / 4632942 / 4633086 / LG6 / 100 / 81,00 / 6,E-04 / 737 / 836 / 31780853 / 31780952
108J24T7 / 840 / MDC022311.86 - chr17 / 103 / 82,00 / 2,E-13 / 207 / 308 / 5163109 / 5163007
108J24T7 / 840 / MDC022311.86 - chr17 / 115 / 84,00 / 2,E-13 / 433 / 544 / 5162868 / 5162754
108J24T7 / 840 / MDC022311.86 - chr17 / 102 / 83,00 / 2,E-10 / 735 / 836 / 5162662 / 5162561
224A03 / 224A03SP6 / 684 / scaffold_3 / 258 / 98,45 / 1,E-134 / 231 / 488 / 18509654 / 18509911 / MDC021594.239 - chr9 / 33 / 90,91 / 1,E-01 / 372 / 404 / 4635895 / 4635966 / LG6 / 54 / 90,74 / 5,E-10 / 585 / 638 / 31778037 / 31778090
224A03SP6 / 684 / MDC021594.239 - chr9 / 72 / 94,44 / 2,E-22 / 583 / 654 / 4636136 / 4636168
224A03T7 / 875 / scaffold_3 / 577 / 93,24 / 0,E+00 / 87 / 644 / 18559191 / 18559767 / MDC013813.221 - chr17 / 59 / 88,14 / 2,E-07 / 174 / 232 / 5134295 / 5134353
224A03T7 / 875 / MDC013813.221 - chr17 / 94 / 82,98 / 4,E-02 / 421 / 510 / 5134068 / 5134160
234O11 / 234O11T7 / 894 / scaffold_3 / 895 / 97,88 / 0,E+00 / 1 / 894 / 18538276 / 18539170 / MDC022311.89 - chr9 / 889 / 89,20 / 0,E+00 / 1 / 888 / 4610846 / 4611734 / LG6 / 865 / 88,44 / 0,E+00 / 30 / 894 / 31806535 / 31807396
234O11T7 / 894 / MDC012046.320 - chr9 / 889 / 90,21 / 0,E+00 / 1 / 888 / 4605146 / 4606034
234O11T7 / 894 / MDC013246.168 - chr17 / 892 / 90,02 / 0,E+00 / 1 / 888 / 5159480 / 5160370
234O11SP6 / 865 / scaffold_3 / 809 / 93,08 / 0,E+00 / 12 / 812 / 18608700 / 18609506 / MDC015700.126 - chr17 / 52 / 98,08 / 3,E-15 / 348 / 398 / 5049140 / 5049191
234O11SP6 / 865 / MDC015700.126 - chr17 / 88 / 85,23 / 3,E-06 / 411 / 497 / 5049206 / 5049291
234O11SP6 / 865 / MDC015700.126 - chr17 / 52 / 90,38 / 4,E-08 / 670 / 721 / 5049471 / 5049522
148M17 / 148M17T7 / 1048 / scaffold_3 / 318 / 91,19 / 1,E-110 / 458 / 774 / 18551274 / 18551591
148M17SP6 / 786 / scaffold_3 / 669 / 93,27 / 0,E+00 / 128 / 781 / 18660261 / 18660925 / MDC022667.46 - chr17 / 83 / 85,54 / 3,E-12 / 9 / 91 / 5008837 / 5008755 / LG6 / 103 / 87,38 / 4,E-20 / 172 / 274 / 31900308 / 31900410
148M17SP6 / 786 / MDC022667.46 - chr17 / 115 / 91,30 / 8,E-34 / 161 / 275 / 5008640 / 5008526
148M17SP6 / 786 / MDC022667.46 - chr17 / 94 / 92,55 / 5,E-26 / 322 / 414 / 5008445 / 5008352
253J12 / 253J12SP6 / 917 / scaffold_3 / 909 / 97,69 / 0,E+00 / 9 / 917 / 18667427 / 18668334 / MDC005044.383 - chr9 / 298 / 86,24 / 2,E-72 / 239 / 536 / 4334028 / 4334325 / LG6 / 298 / 85,23 / 3,E-61 / 239 / 533 / 31892305 / 31892602
253J12SP6 / 917 / MDC020502.76 - chr17 / 298 / 85,23 / 2,E-65 / 239 / 536 / 4976148 / 4976445
253J12SP6 / 917 / MDC002854.200 - chr17 / 298 / 85,57 / 4,E-64 / 239 / 536 / 4976403 / 4976700
253J12T7 / 924 / scaffold_3 / 377 / 91,78 / 1,E-137 / 1 / 367 / 18634126 / 18634501 / LG6 / 48 / 91,67 / 1,E-08 / 507 / 554 / 31879131 / 31879178
253J12T7 / 924 / scaffold_3 / 355 / 90,14 / 1,E-122 / 417 / 754 / 18634504 / 18634858
251L05 / 251L05SP6 / 1166 / scaffold_3 / 238 / 88,24 / 1,E-70 / 17 / 244 / 18657325 / 18657562 / MDC002854.297 - chr9 / 117 / 88,03 / 2,E-20 / 106 / 221 / 4356598 / 4356708 / LG6 / 20 / 100,00 / 2,E-01 / 128 / 147 / 31905055 / 31905074
251L05SP6 / 1166 / scaffold_3 / 707 / 91,80 / 0,E+00 / 320 / 1018 / 18657627 / 18658310 / MDC002854.297 - chr9 / 42 / 95,24 / 3,E-09 / 629 / 670 / 4357117 / 4357158 / LG6 / 27 / 96,30 / 3,E-03 / 195 / 221 / 31904969 / 31904995
251L05SP6 / 1166 / MDC002854.297 - chr9 / 60 / 98,33 / 3,E-22 / 689 / 748 / 4357189 / 4357248 / LG6 / 24 / 95,83 / 2,E-01 / 499 / 522 / 31904812 / 31904835
251L05SP6 / 1166 / MDC022667.46 - chr17 / 116 / 89,66 / 1,E-21 / 106 / 221 / 4999458 / 4999566 / LG6 / 51 / 96,08 / 4,E-15 / 693 / 743 / 31904532 / 31904582
251L05SP6 / 1166 / MDC022667.46 - chr17 / 24 / 95,83 / 8,E-01 / 507 / 530 / 4999892 / 4999915
251L05SP6 / 1166 / MDC022667.46 - chr17 / 43 / 93,02 / 2,E-07 / 628 / 670 / 4999984 / 5000026
251L05SP6 / 1166 / MDC022667.46 - chr17 / 60 / 98,33 / 3,E-22 / 689 / 748 / 5000057 / 5000116
251L05T7 / 1022 / scaffold_2 / 206 / 84,47 / 1,E-45 / 1 / 195 / 21838466 / 21838671 / MDC017251.268 - chr1 / 82 / 84,15 / 8,E-07 / 718 / 799 / 17106807 / 17106726 / LG7 / 87 / 81,61 / 3,E-03 / 715 / 801 / 17549587 / 17549673
251L05T7 / 1022 / scaffold_2 / 90 / 94,44 / 3,E-31 / 106 / 195 / 21832192 / 21832281 / LG7 / 89 / 83,15 / 8,E-07 / 715 / 803 / 17675037 / 17675125
251L05T7 / 1022 / scaffold_2 / 62 / 87,10 / 2,E-07 / 117 / 178 / 21829572 / 21829633 / LG7 / 83 / 81,93 / 3,E-03 / 717 / 799 / 17690062 / 17690144
251L05T7 / 1022 / scaffold_2 / 419 / 89,02 / 1,E-128 / 235 / 640 / 21836182 / 21836595 / LG7 / 78 / 85,90 / 9,E-10 / 721 / 798 / 17683914 / 17683991
251L05T7 / 1022 / scaffold_2 / 208 / 92,79 / 3,E-77 / 644 / 847 / 21836643 / 21836850 / LG7 / 60 / 86,67 / 3,E-06 / 759 / 818 / 17545396 / 17545455
251L05T7 / 1022 / scaffold_2 / 188 / 94,68 / 2,E-75 / 660 / 847 / 21840103 / 21840289 / LG7 / 60 / 86,67 / 3,E-06 / 759 / 818 / 17679693 / 17679752
251L05T7 / 1022 / scaffold_2 / 84 / 83,33 / 3,E-06 / 762 / 845 / 21835192 / 21835275 / LG7 / 45 / 88,89 / 2,E-04 / 765 / 809 / 17687594 / 17687638
251L05T7 / 1022 / scaffold_2 / 118 / 88,14 / 2,E-23 / 841 / 957 / 21836949 / 21837065
251L05T7 / 1022 / scaffold_2 / 142 / 90,85 / 1,E-39 / 841 / 981 / 21840388 / 21840528
251L05T7 / 1022 / scaffold_2 / 88 / 98,86 / 1,E-39 / 108 / 195 / 24324974 / 24325061
251L05T7 / 1022 / scaffold_2 / 61 / 91,80 / 6,E-14 / 117 / 177 / 24327209 / 24327269
251L05T7 / 1022 / scaffold_2 / 251 / 94,42 / 1,E-106 / 394 / 644 / 24321077 / 24321327
160J21 / 160J21SP6 / 1079 / scaffold_3 / 512 / 90,23 / 0,E+00 / 10 / 521 / 18770552 / 18771063
160J21SP6 / 1079 / scaffold_3 / 331 / 88,22 / 3,E-96 / 527 / 856 / 18770182 / 18770499
160J21SP6 / 1079 / scaffold_3 / 119 / 94,12 / 2,E-41 / 960 / 1078 / 18769556 / 18769673
160J21T7 / 1118 / scaffold_2 / 558 / 89,78 / 0,E+00 / 382 / 938 / 21840139 / 21840695 / MDC017637.280 - chr1 / 59 / 84,75 / 1,E-02 / 383 / 441 / 17244888 / 17244830 / LG7 / 134 / 82,84 / 1,E-14 / 287 / 420 / 17687062 / 17687195
160J21T7 / 1118 / MDC017637.280 - chr1 / 36 / 91,67 / 3,E-03 / 514 / 549 / 17244751 / 17244716 / LG7 / 49 / 89,80 / 9,E-07 / 686 / 734 / 17687455 / 17687503
160J21T7 / 1118 / MDC017637.280 - chr1 / 91 / 83,52 / 2,E-07 / 791 / 881 / 17244515 / 17244425 / LG7 / 75 / 86,67 / 2,E-10 / 795 / 869 / 17687564 / 17687638
161F24 / 161F24SP6 / 1076 / scaffold_3 / 423 / 95,74 / 0,E+00 / 621 / 1037 / 18748297 / 18748719
161F24T7 / 1113 / scaffold_3 / 252 / 90,87 / 1,E-82 / 26 / 277 / 18832742 / 18832992
161F24T7 / 1113 / scaffold_3 / 99 / 90,91 / 4,E-27 / 315 / 413 / 18832637 / 18832735
161F24T7 / 1113 / scaffold_3 / 276 / 88,04 / 3,E-71 / 381 / 649 / 18828557 / 18828831
161F24T7 / 1113 / scaffold_3 / 263 / 91,63 / 6,E-88 / 443 / 701 / 18414075 / 18414336
161F24T7 / 1113 / scaffold_3 / 76 / 89,47 / 2,E-13 / 476 / 550 / 18832528 / 18832603
161F24T7 / 1113 / scaffold_3 / 64 / 98,44 / 3,E-25 / 650 / 713 / 18832133 / 18832196
161F24T7 / 1113 / scaffold_3 / 119 / 91,60 / 5,E-33 / 862 / 980 / 18414464 / 18414580
217G17 / 217G17SP6 / 684 / scaffold_3 / 672 / 99,11 / 0,E+00 / 13 / 684 / 18530382 / 18531052 / MDC012046.320 - chr9 / 670 / 91,04 / 0,E+00 / 15 / 684 / 4612489 / 4613157 / LG6 / 665 / 90,08 / 0,E+00 / 20 / 684 / 31798038 / 31798701
217G17SP6 / 684 / MDC022311.89 - chr9 / 640 / 90,16 / 0,E+00 / 45 / 684 / 4603644 / 4604282
217G17SP6 / 684 / MDC013246.168 - chr17 / 670 / 91,19 / 0,E+00 / 15 / 684 / 5152273 / 5152941
217G17T7 / 853 / scaffold_3 / 350 / 90,86 / 1,E-116 / 4 / 346 / 18596437 / 18596778 / MDC015023.449 - chr9 / 112 / 85,71 / 1,E-23 / 579 / 690 / 4439790 / 4439901 / LG6 / 59 / 88,14 / 4,E-08 / 570 / 628 / 31847476 / 31847534
217G17T7 / 853 / scaffold_3 / 484 / 92,15 / 0,E+00 / 368 / 847 / 18595981 / 18596449 / MDC015023.449 - chr9 / 45 / 88,89 / 6,E-04 / 603 / 647 / 4442270 / 4442314 / LG6 / 63 / 88,89 / 2,E-10 / 669 / 731 / 31843483 / 31843545
217G17T7 / 853 / MDC008853.340 - chr9 / 125 / 84,00 / 2,E-19 / 566 / 690 / 4466348 / 4466471
217G17T7 / 853 / MDC008853.340 - chr9 / 36 / 91,67 / 2,E-03 / 612 / 647 / 4463960 / 4463995
217G17T7 / 853 / MDC005795.287 - chr17 / 84 / 88,10 / 2,E-16 / 566 / 649 / 5064737 / 5064820
217G17T7 / 853 / MDC005795.287 - chr17 / 45 / 88,89 / 6,E-04 / 603 / 647 / 5062205 / 5062249
170A18 / 170A18SP6 / 926 / scaffold_3 / 930 / 95,59 / 0,E+00 / 7 / 926 / 18650125 / 18651052 / MDC016663.182 - chr9 / 66 / 90,91 / 5,E-14 / 1 / 66 / 4381192 / 4381257 / LG6 / 160 / 89,38 / 2,E-44 / 514 / 673 / 31914431 / 31914590
170A18SP6 / 926 / MDC016663.182 - chr9 / 189 / 86,77 / 3,E-43 / 152 / 340 / 4381502 / 4381690 / LG6 / 173 / 87,86 / 1,E-42 / 150 / 322 / 31914068 / 31914240
170A18SP6 / 926 / MDC016663.182 - chr9 / 262 / 87,02 / 4,E-64 / 412 / 670 / 4381757 / 4382018 / LG6 / 103 / 89,32 / 8,E-25 / 771 / 873 / 31914650 / 31914752
170A18SP6 / 926 / MDC016663.182 - chr9 / 119 / 89,92 / 2,E-32 / 758 / 876 / 4382087 / 4382205 / LG6 / 64 / 90,63 / 2,E-13 / 1 / 64 / 31913943 / 31914006
170A18SP6 / 926 / MDC008853.341 - chr17 / 45 / 95,56 / 5,E-11 / 21 / 65 / 5015162 / 5015206
170A18SP6 / 926 / MDC008853.341 - chr17 / 204 / 87,25 / 1,E-48 / 139 / 342 / 5015351 / 5015554
170A18SP6 / 926 / MDC008853.341 - chr17 / 262 / 87,02 / 6,E-63 / 412 / 670 / 5015618 / 5015879
170A18SP6 / 926 / MDC008853.341 - chr17 / 95 / 88,42 / 2,E-19 / 772 / 866 / 5015961 / 5016055
170A18T7 / 919 / scaffold_3 / 589 / 91,85 / 0,E+00 / 28 / 614 / 18628332 / 18628911
170A18T7 / 919 / scaffold_3 / 308 / 97,08 / 1,E-152 / 612 / 919 / 18628958 / 18629265
200J16 / 200J16SP6 / 866 / scaffold_3 / 626 / 94,73 / 0,E+00 / 10 / 627 / 18652749 / 18653374 / MDC016663.182 - chr9 / 154 / 90,26 / 6,E-41 / 33 / 185 / 4377538 / 4377689 / LG6 / 64 / 85,94 / 6,E-04 / 56 / 113 / 31910805 / 31910868
200J16SP6 / 866 / MDC016663.182 - chr9 / 54 / 87,04 / 1,E-02 / 496 / 549 / 4378706 / 4378757 / LG6 / 23 / 100,00 / 2,E-03 / 163 / 185 / 31910943 / 31910965
200J16SP6 / 866 / MDC016663.182 - chr9 / 44 / 88,64 / 4,E-02 / 560 / 603 / 4378761 / 4378803 / LG6 / 66 / 84,85 / 9,E-03 / 472 / 536 / 31911362 / 31911427
200J16SP6 / 866 / MDC003754.233 - chr9 / 154 / 90,26 / 6,E-41 / 33 / 185 / 4372977 / 4373128
200J16SP6 / 866 / MDC022667.46 - chr17 / 145 / 90,34 / 2,E-38 / 33 / 177 / 4993037 / 4993177
200J16SP6 / 866 / MDC022667.46 - chr17 / 53 / 90,57 / 1,E-08 / 467 / 519 / 4991584 / 4991636
200J16SP6 / 866 / MDC022667.46 - chr17 / 33 / 96,97 / 3,E-06 / 595 / 627 / 4991485 / 4991517
200J16T7 / 826 / scaffold_3 / 379 / 92,08 / 1,E-144 / 126 / 504 / 18632682 / 18633040 / MDC015700.127 - chr9 / 132 / 93,94 / 1,E-48 / 128 / 259 / 4398545 / 4398676 / LG6 / 122 / 90,98 / 3,E-36 / 132 / 253 / 31877185 / 31877306
200J16T7 / 826 / scaffold_3 / 289 / 97,23 / 1,E-141 / 537 / 823 / 18633076 / 18633364 / MDC015700.127 - chr9 / 147 / 90,48 / 2,E-43 / 356 / 502 / 4398245 / 4398391 / LG6 / 139 / 94,24 / 2,E-53 / 361 / 499 / 31877398 / 31877536
200J16T7 / 826 / MDC015700.127 - chr9 / 113 / 88,50 / 2,E-25 / 576 / 688 / 4398039 / 4398151 / LG6 / 108 / 86,11 / 3,E-18 / 573 / 680 / 31877793 / 31877900
200J16T7 / 826 / MDC009533.88 - chr9 / 132 / 92,42 / 3,E-46 / 128 / 259 / 4398056 / 4398187
200J16T7 / 826 / MDC009533.88 - chr9 / 147 / 90,48 / 2,E-43 / 356 / 502 / 4397767 / 4397913
200J16T7 / 826 / MDC009533.88 - chr9 / 113 / 88,50 / 2,E-25 / 576 / 688 / 4397561 / 4397673
200J16T7 / 826 / MDC008853.341 - chr17 / 122 / 94,26 / 4,E-45 / 138 / 259 / 5032618 / 5032739
200J16T7 / 826 / MDC008853.341 - chr17 / 147 / 91,84 / 4,E-48 / 356 / 502 / 5032370 / 5032516
200J16T7 / 826 / MDC008853.341 - chr17 / 95 / 92,63 / 5,E-29 / 594 / 688 / 5032087 / 5032181
13N08 / 13N08SP6 / 957 / scaffold_3 / 944 / 97,25 / 0,E+00 / 14 / 954 / 18667422 / 18668364 / MDC005044.383 - chr9 / 298 / 86,24 / 2,E-72 / 271 / 568 / 4334028 / 4334325 / LG6 / 298 / 85,23 / 3,E-61 / 271 / 565 / 31892305 / 31892602
13N08SP6 / 957 / MDC005044.383 - chr9 / 243 / 86,83 / 6,E-60 / 607 / 849 / 4334362 / 4334604
13N08SP6 / 957 / MDC020502.76 - chr17 / 51 / 88,24 / 5,E-05 / 177 / 227 / 4976058 / 4976108
13N08SP6 / 957 / MDC020502.76 - chr17 / 298 / 85,23 / 2,E-65 / 271 / 568 / 4976148 / 4976445
13N08SP6 / 957 / MDC020502.76 - chr17 / 249 / 87,00 / 9,E-59 / 610 / 858 / 4976488 / 4976736
13N08SP6 / 957 / MDC020502.76 - chr17 / 30 / 93,33 / 4,E-02 / 901 / 930 / 4976782 / 4976811
13N08T7 / 883 / scaffold_3 / 809 / 93,08 / 0,E+00 / 14 / 816 / 18625646 / 18626450
7M17 / 7M17SP6 / 825 / scaffold_3 / 504 / 91,67 / 0,E+00 / 13 / 512 / 18656522 / 18657016 / MDC022667.46 -chr17 / 71 / 88,73 / 3,E-12 / 384 / 454 / 4998311 / 4998241
7M17T7 / 981 / scaffold_3 / 897 / 92,31 / 0,E+00 / 12 / 907 / 18618174 / 18619069
7M17T7 / 981 / scaffold_2 / 624 / 81,41 / 4,E-73 / 84 / 691 / 212022 / 212641
7M17T7 / 981 / scaffold_4 / 507 / 80,28 / 3,E-52 / 287 / 777 / 13598462 / 13598966
7M17T7 / 981 / scaffold_1 / 322 / 81,68 / 1,E-33 / 388 / 707 / 16362701 / 16363021
59M14 / 59M14T7 / 895 / scaffold_3 / 533 / 97,75 / 0,E+00 / 1 / 533 / 18639978 / 18640510 / MDC009533.88 - chr9 / 370 / 84,86 / 1,E-79 / 1 / 370 / 4393000 / 4393369 / LG6 / 159 / 81,13 / 7,E-13 / 26 / 184 / 31882800 / 31882958
59M14T7 / 895 / scaffold_3 / 359 / 98,61 / 0,E+00 / 537 / 895 / 18640562 / 18640920 / MDC008853.341 - chr17 / 346 / 87,28 / 5,E-88 / 25 / 370 / 5026243 / 5026586 / LG6 / 210 / 83,33 / 3,E-30 / 137 / 346 / 31883007 / 31883213
59M14T7 / 895 / LG6 / 100 / 94,00 / 6,E-35 / 434 / 533 / 31883300 / 31883399
59M14T7 / 895 / LG6 / 143 / 95,10 / 3,E-58 / 700 / 842 / 31883670 / 31883812
59M14SP6 / 864 / scaffold_3 / 304 / 91,78 / 1,E-108 / 17 / 318 / 18665150 / 18665445
164D09 / 164D09T7 / 795 / scaffold_3 / 698 / 95,70 / 0,E+00 / 1 / 698 / 18754718 / 18755406 / MDC018769.107 - chr9 / 326 / 88,65 / 2,E-96 / 351 / 676 / 4322190 / 4322515 / LG6 / 239 / 86,19 / 2,E-53 / 343 / 581 / 31930040 / 31930278
164D09T7 / 795 / MDC018769.120 - chr17 / 318 / 89,62 / 1,E-101 / 351 / 668 / 4968973 / 4968656
164D09SP6 / 791 / scaffold_2 / 400 / 96,00 / 0,E+00 / 21 / 420 / 24326400 / 24326797
164D09SP6 / 791 / scaffold_2 / 400 / 95,50 / 0,E+00 / 21 / 420 / 21829989 / 21830386
164D09SP6 / 791 / scaffold_6 / 475 / 92,84 / 0,E+00 / 21 / 493 / 23333992 / 23334461
164D09SP6 / 791 / scaffold_6 / 473 / 91,33 / 1,E-171 / 21 / 493 / 25009793 / 25010250
65P22 / 65P22SP6 / 856 / scaffold_3 / 849 / 92,93 / 0,E+00 / 12 / 855 / 18768746 / 18769594 / MDC000935.644 - chr17 / 274 / 87,59 / 1,E-73 / 582 / 855 / 4946703 / 4946976 / LG6 / 80 / 86,25 / 4,E-11 / 773 / 852 / 31943999 / 31944078
65P22SP6 / 856 / scaffold_3 / 274 / 83,58 / 1,E-45 / 582 / 855 / 18808399 / 18808672 / MDC018769.116 - chr17 / 273 / 87,91 / 4,E-73 / 582 / 854 / 4937933 / 4938205
65P22SP6 / 856 / scaffold_3 / 260 / 79,62 / 3,E-18 / 596 / 855 / 18409409 / 18409668 / MDC018769.116 - chr17 / 273 / 87,55 / 4,E-73 / 582 / 854 / 4951124 / 4951396
65P22SP6 / 856 / MDC011932.254 - chr17 / 263 / 81,75 / 9,E-34 / 593 / 855 / 4923208 / 4923470
65P22SP6 / 856 / MDC000935.617 - chr17 / 263 / 81,75 / 2,E-31 / 593 / 855 / 4927036 / 4927298
65P22SP6 / 856 / MDC012367.436 - chr17 / 263 / 79,85 / 3,E-24 / 593 / 855 / 4722332 / 4722594
65P22SP6 / 856 / MDC000935.643 - chr17 / 263 / 80,23 / 5,E-23 / 593 / 855 / 4736035 / 4736297
65P22SP6 / 856 / MDC002825.324 - chr6 / 263 / 79,85 / 1,E-20 / 593 / 855 / 21188310 / 21188572
65P22SP6 / 856 / MDC010466.192 - chr6 / 266 / 79,32 / 2,E-16 / 590 / 855 / 21196810 / 21197075
65P22SP6 / 856 / MDC020813.183 - chr2 / 263 / 80,61 / 3,E-24 / 593 / 855 / 18791195 / 18791457
65P22T7 / 808 / scaffold_2 / 251 / 94,42 / 1,E-106 / 478 / 728 / 24321077 / 24321327 / MDC015867.201 - chr7 / 59 / 94,92 / 4,E-17 / 9 / 67 / 21017158 / 21017100 / LG7 / 66 / 93,94 / 2,E-19 / 2 / 67 / 16076687 / 16076752
65P22T7 / 808 / scaffold_2 / 299 / 90,97 / 1,E-102 / 482 / 780 / 21836300 / 21836595
198D13 / 198D13SP6 / 729 / scaffold_3 / 726 / 96,83 / 0,E+00 / 7 / 729 / 19014526 / 19015245 / MDC011658.303 - chr9 / 287 / 82,58 / 9,E-43 / 443 / 729 / 4074206 / 4074486
198D13SP6 / 729 / MDC011658.302 - chr9 / 279 / 82,08 / 3,E-33 / 443 / 720 / 4102012 / 4102283
198D13T7 / 813 / scaffold_3 / 314 / 94,59 / 1,E-131 / 3 / 313 / 18843157 / 18843466 / MDC020813.184 - chr9 / 38 / 89,47 / 9,E+00 / 12 / 48 / 4241939 / 4241902
198D13T7 / 813 / scaffold_3 / 445 / 93,26 / 0,E+00 / 374 / 813 / 18843527 / 18843970 / MDC020813.184 - chr9 / 30 / 96,67 / 2,E-04 / 440 / 469 / 4241545 / 4241516
198D13T7 / 813 / MDC020813.184 - chr9 / 38 / 89,47 / 9,E+00 / 755 / 791 / 4241480 / 4241443
198D13T7 / 813 / MDC018983.157 - chr17 / 18 / 100,00 / 9,E+00 / 33 / 50 / 4713164 / 4713181
198D13T7 / 813 / MDC018983.157 - chr17 / 68 / 88,24 / 2,E-10 / 402 / 469 / 4713481 / 4713548
148J08 / 148J08T7 / 885 / scaffold_3 / 889 / 98,54 / 0,E+00 / 1 / 885 / 19425951 / 19426839 / MDC015186.67 - chr9 / 244 / 90,98 / 2,E-84 / 1 / 244 / 3544359 / 3544116 / LG6 / 244 / 85,66 / 1,E-51 / 1 / 244 / 32598153 / 32598396
148J08T7 / 885 / MDC015186.67 - chr9 / 300 / 88,33 / 2,E-84 / 386 / 685 / 3544046 / 3543747 / LG6 / 296 / 89,19 / 7,E-90 / 394 / 689 / 32597780 / 32598075
148J08T7 / 885 / MDC015186.72 - chr17 / 244 / 92,21 / 2,E-90 / 1 / 244 / 3858384 / 3858141
148J08T7 / 885 / MDC015186.72 - chr17 / 299 / 88,29 / 3,E-86 / 387 / 685 / 3858070 / 3857772
148J08SP6 / 683 / scaffold_3 / 35 / 97,14 / 3,E-08 / 109 / 143 / 19357752 / 19357786
148J08SP6 / 683 / scaffold_3 / 35 / 91,43 / 2,E-03 / 636 / 670 / 19291681 / 19291715
12J14 / 12J14SP6 / 1061 / scaffold_3 / 472 / 93,43 / 0,E+00 / 15 / 485 / 18615545 / 18615074
12J14SP6 / 1061 / scaffold_3 / 81 / 96,30 / 1,E-30 / 594 / 674 / 18614933 / 18614853
12J14SP6 / 1061 / scaffold_3 / 144 / 87,50 / 2,E-32 / 671 / 814 / 18614837 / 18614694
12J14SP6 / 1061 / scaffold_3 / 240 / 96,25 / 2,E-63 / 812 / 1048 / 18614399 / 18614160
12J14T7 / 981 / scaffold_3 / 726 / 92,70 / 0,E+00 / 256 / 981 / 18583563 / 18584287 / MDC021662.249 - chr17 / 130 / 93,85 / 5,E-45 / 5 / 133 / 5081892 / 5081763 / LG6 / 134 / 88,06 / 5,E-34 / 3 / 131 / 31832528 / 31832657
12J14T7 / 981 / scaffold_3 / 243 / 95,06 / 1,E-107 / 1 / 242 / 18583297 / 18583539 / MDC021662.249 - chr17 / 71 / 85,92 / 2,E-07 / 661 / 731 / 5081261 / 5081191 / LG6 / 173 / 81,50 / 9,E-29 / 816 / 981 / 31833287 / 31833449
12J14T7 / 981 / MDC021662.249 - chr17 / 220 / 85,45 / 3,E-49 / 762 / 981 / 5081181 / 5080962
12J14T7 / 981 / MDC020364.508 - chr17 / 130 / 93,85 / 5,E-45 / 5 / 133 / 5082222 / 5082093
12J14T7 / 981 / MDC020364.508 - chr17 / 34 / 91,18 / 5,E-02 / 698 / 731 / 5080760 / 5080727
12J14T7 / 981 / MDC020364.508 - chr17 / 221 / 85,07 / 5,E-45 / 762 / 981 / 5080717 / 5080497
210A19 / 210A19SP6 / 1037 / scaffold_3 / 84 / 90,48 / 2,E-19 / 97 / 177 / 18545820 / 18545903 / MDC022311.89 - chr9 / 420 / 81,90 / 6,E-57 / 569 / 987 / 4613237 / 4613650 / LG6 / 464 / 79,74 / 1,E-68 / 559 / 1001 / 31808874 / 31809315
210A19SP6 / 1037 / scaffold_3 / 85 / 97,65 / 2,E-35 / 279 / 363 / 18546770 / 18546854 / MDC013246.168 - chr17 / 148 / 89,19 / 3,E-37 / 843 / 989 / 5162126 / 5162273
210A19SP6 / 1037 / scaffold_3 / 319 / 95,92 / 1,E-149 / 428 / 746 / 18546896 / 18547214 / MDC013246.168 - chr17 / 205 / 81,46 / 4,E-24 / 569 / 773 / 5161858 / 5162062
210A19SP6 / 1037 / scaffold_3 / 437 / 92,22 / 1,E-168 / 543 / 978 / 18540641 / 18541071
210A19SP6 / 1037 / scaffold_3 / 232 / 95,26 / 1,E-100 / 780 / 1010 / 18547213 / 18547444
210A19T7 / 1087 / scaffold_3 / 316 / 94,62 / 1,E-130 / 743 / 1053 / 18588978 / 18588664 / LG6 / 110 / 74,55 / 1,E-06 / 361 / 456 / 6169626 / 6169723
210A19T7 / 1087
210A19T7 / 1087
155M12 / 155M12SP6 / 1038 / scaffold_3 / 503 / 97,42 / 0,E+00 / 11 / 512 / 18753247 / 18753748 / MDC019670.389 - chr17 / 131 / 84,73 / 1,E-20 / 90 / 220 / 4962732 / 4962862 / LG6 / 401 / 73,07 / 1,E-38 / 87 / 457 / 31928788 / 31929139
155M12SP6 / 1038 / scaffold_3 / 239 / 95,00 / 3,E-99 / 543 / 779 / 18753775 / 18754008 / MDC018769.111 - chr17 / 131 / 84,73 / 1,E-20 / 90 / 220 / 4956370 / 4956240
155M12SP6 / 1038 / scaffold_3 / 214 / 91,59 / 5,E-70 / 811 / 1023 / 18754042 / 18754253
155M12T7 / 1078 / scaffold_2 / 878 / 86,90 / 0,E+00 / 3 / 878 / 7260715 / 72615888 / LG1 / 162 / 70,99 / 5,E-03 / 395 / 538 / 23814021 / 23814166
159P08 / 159P08SP6 / 1098 / scaffold_3 / 340 / 96,76 / 1,E-166 / 12 / 351 / 18770485 / 18770146
159P08SP6 / 1098 / scaffold_3 / 206 / 93,69 / 2,E-81 / 368 / 568 / 18770123 / 18769918
159P08SP6 / 1098 / scaffold_3 / 207 / 93,72 / 6,E-82 / 590 / 796 / 18769907 / 18769701
159P08SP6 / 1098 / scaffold_3 / 202 / 96,53 / 2,E-90 / 842 / 1040 / 18769682 / 18769481
159P08T7 / 1041 / scaffold_2 / 125 / 94,40 / 5,E-45 / 1 / 124 / 21826763 / 21826639 / MDC021893.209 - chr11 / 114 / 96,49 / 2,E-47 / 607 / 720 / 32019116 / 32019229 / LG2 / 447 / 74,94 / 2,E-43 / 569 / 973 / 17093253 / 17093674
159P08T7 / 1041 / scaffold_2 / 181 / 94,48 / 3,E-74 / 548 / 728 / 21846260 / 21846086 / MDC021893.209 - chr11 / 169 / 88,76 / 1,E-42 / 805 / 973 / 32019554 / 32019721 / LG4 / 123 / 76,42 / 8,E-10 / 583 / 698 / 7504798 / 7504907
159P08T7 / 1041 / scaffold_2 / 255 / 92,55 / 6,E-94 / 744 / 997 / 21846053 / 21845800 / MDC009256.211 - chr3 / 114 / 95,61 / 5,E-45 / 607 / 720 / 30907471 / 30907584 / LG4 / 133 / 74,44 / 1,E-06 / 583 / 706 / 7534151 / 7534268
159P08T7 / 1041 / scaffold_2 / 255 / 92,16 / 1,E-91 / 744 / 997 / 24322938 / 24323191 / MDC009256.211 - chr3 / 175 / 88,00 / 2,E-41 / 787 / 961 / 30907868 / 30908041
95D02 / 95D02SP6 / 1050 / scaffold_3 / 1041 / 94,62 / 0,E+00 / 12 / 1050 / 18762670 / 18761631 / MDC019670.425 - chr6 / 338 / 82,25 / 2,E-50 / 15 / 352 / 21214219 / 21213882 / LG6 / 491 / 77,19 / 2,E-52 / 15 / 477 / 31943371 / 31943836
95D02SP6 / 1050 / MDC002001.719 - chr14 / 338 / 82,25 / 2,E-50 / 15 / 352 / 26232921 / 26233258
95D02T7 / 974 / scaffold_2 / 221 / 95,48 / 4,E-95 / 1 / 221 / 24327087 / 24327302 / LG7 / 205 / 81,46 / 1,E-27 / 376 / 574 / 17687457 / 17687652
95D02T7 / 974 / scaffold_2 / 115 / 95,65 / 3,E-46 / 242 / 356 / 24327319 / 24327433 / LG7 / 101 / 85,15 / 1,E-14 / 373 / 471 / 17549439 / 17549537
95D02T7 / 974 / scaffold_2 / 211 / 94,31 / 8,E-87 / 364 / 574 / 24327759 / 24327969 / LG7 / 200 / 70,50 / 9,E-08 / 393 / 569 / 17677288 / 17677462
95D02T7 / 974 / scaffold_2 / 265 / 94,34 / 1,E-113 / 579 / 835 / 24317690 / 24317954
95D02T7 / 974 / scaffold_2 / 185 / 95,68 / 8,E-81 / 654 / 838 / 24328745 / 24328929
95D02T7 / 974 / scaffold_5 / 96 / 92,71 / 4,E-30 / 261 / 356 / 14120025 / 14119930
95D02T7 / 974 / scaffold_5 / 205 / 95,61 / 2,E-90 / 365 / 569 / 14119559 / 14119355
95D02T7 / 974 / scaffold_5 / 261 / 96,55 / 1,E-121 / 579 / 838 / 14117549 / 14117289

Supplemental Table 6.- Gene content of the M-locus peach syntenic region. Position and length of the ORFs as well as the first BLASTP match on the TAIR database annotated by IPGI are shown. Overlap length (amino acids), percent id and E-value are indicated for each Prunus/Arabidopsis gene pair. Arabidopsis homologues with detectable expression in mature pollen, hydrated pollen and pollen tubes (+/-) and those with altered transcription during pollen germination (PG) or pollen tube growth (PTG) are also indicated according to the results reported by Wang et al. (2008) using Affymetrix ATH1 Genome Arrays.

Peach Gene ID / Transcript
Start Stop Length / TAIR / Description / Overlap
length / % id / E-value / Pollen expr. / Altered transc.
ppa017286m / 18403596 / 18405133 / 1538 / AT5G07610.1a / F-box family protein / 331 / 21 / 0.001 / - / -
ppa019630m / 18409000 / 18413037 / 4038 / AT3G51550.1 / FER (FERONIA); kinase/ protein kinase / 833 / 49,7 / 0 / - / -
ppa007129m / 18416998 / 18418727 / 1730 / AT3G02340.1b / zinc finger (C3HC4-type RING finger) family protein / 275 / 38,18 / 3E-40 / + / down PTG
ppa018733m / 18419306 / 18421190 / 1885 / AT5G26667.3 / PYR6; cytidylate kinase/ uridylate kinase / 200 / 59 / 9E-64 / - / -
ppa000048m / 18423078 / 18435264 / 12187 / AT5G15680.1b / binding / 2158 / 87,21 / 0 / - / -
ppa005464m / 18435743 / 18438849 / 3107 / AT3G30390.2b / amino acid transporter family protein / 440 / 76,36 / 6E-179 / + / /
ppa006871m / 18435743 / 18438849 / 3107 / AT5G38820.1 / amino acid transporter family protein / 441 / 57,82 / 4E-128 / + / down PG
ppa002236m / 18441946 / 18446012 / 4067 / AT5G11390.1b / unknown protein / 719 / 46,31 / 1E-145 / + / Up PTG
ppa019428m / 18452230 / 18454181 / 1952 / AT3G30390.2 / amino acid transporter family protein / 434 / 54,15 / 4E-133 / + / /
ppa021437m / 18455117 / 18457128 / 2012 / AT3G30390.2 / amino acid transporter family protein / 412 / 57,77 / 9E-128 / + / /
ppa013493m / 18457877 / 18458491 / 615 / AT5G49550.1b / unknown protein / 112 / 69,64 / 9E-42 / + / down PTG
ppa003127m / 18458829 / 18461212 / 2384 / AT3G29290.1b / emb2076
(embryo defective 2076) / 448 / 61,83 / 6E-160 / - / -
ppa002013m / 18461451 / 18466635 / 5185 / AT5G38840.1b / forkhead-associated domain-containing protein / FHA domain-containing protein / 681 / 53,89 / 9E-156 / - / -
ppa000719m / 18467901 / 18475951 / 8051 / AT5G15700.1b / DNA-directed RNA polymerase (RPOT2) / 1035 / 67,05 / 0 / - / -
ppa007239m / 18477605 / 18480424 / 2820 / AT3G30380.1b / unknown protein / 368 / 73,37 / 1E-143 / + / down PTG
ppa005877m / 18484708 / 18486428 / 1721 / AT5G15710.1b / F-box family protein / 407 / 87,96 / 0 / - / -
ppa015253m / 18489152 / 18490853 / 1702 / AT4G21900.1a / PRORP3/ proteinaceous RNase P3 / 88 / 26 / 1.4 / - / -
ppa022538m / 18491554 / 18493033 / 1480 / AT5G15720.1 / GLIP7; carboxylesterase/ lipase / 340 / 44,71 / 1E-76 / - / -
ppa006182m / 18495042 / 18498361 / 3320 / AT5G15730.2b / serine/threonine protein kinase, putative / 428 / 66,36 / 3E-155 / - / -
ppa002721m / 18498827 / 18500772 / 1946 / AT2G03890.1 / phosphatidylinositol 3- and 4-kinase family protein / 660 / 63,79 / 0 / - / -
ppa002370m / 18503945 / 18508593 / 4649 / AT3G30300.1b / unknown protein / 690 / 68,41 / 0 / - / -
ppa008856m / 18510031 / 18513228 / 3198 / AT1G69010.1 / BIM2 (BES1-interacting Myc-like protein 2); DNA binding / transcription factor / 273 / 44,32 / 5E-43 / - / -
ppa004594m / 18515587 / 18518005 / 2419 / AT5G15740.1 / unknown protein / 508 / 69,09 / 0 / - / -
ppa012139m / 18522087 / 18523783 / 1697 / AT5G15750.1b / RNA-binding S4 domain-containing protein / 167 / 80,84 / 1E-80 / - / -
ppa000002m / 18524173 / 18545067 / 20895 / AT3G02260.1b / BIG (BIG); binding / ubiquitin-protein ligase/ zinc ion binding / 5008 / 68,11 / 0 / + / /
ppa026731m / 18545106 / 18546555 / 1450 / AT3G25270.1 / nucleic acid binding / 149 / 28,86 / 1E-10 / - / -
ppa023507m / 18559505 / 18560630 / 1126 / AT4G37180.1 / myb family transcription factor / 52 / 67,31 / 2E-14 / + / down PTG
ppa005351m / 18563326 / 18565121 / 1796 / AT5G15780.1 / pollen Ole e 1 allergen and extensin family protein / 168 / 58,93 / 9E-41 / - / -
ppa011450m / 18574543 / 18575507 / 965 / N/A / N/A / N/A / N/A / N/A / N/A / N/A
ppa001620m / 18581982 / 18588641 / 6660 / AT5G38880.1b / unknown protein / 794 / 74,31 / 0 / - / -
ppa011007m / 18590528 / 18594247 / 3720 / AT5G15790.2b / zinc finger (C3HC4-type RING finger) family protein / 231 / 60,61 / 3E-74 / - / -
ppa017665m / 18594777 / 18596276 / 1500 / AT5G38900.1 / DSBA oxidoreductase family protein / 207 / 55,07 / 2E-67 / + / /
ppa011285m / 18597087 / 18599509 / 2423 / AT5G38900.1 / DSBA oxidoreductase family protein / 214 / 63,08 / 6E-77 / + / /
ppa011289m / 18597087 / 18599313 / 2227 / AT5G38900.1 / DSBA oxidoreductase family protein / 214 / 63,08 / 6E-77 / + / /
ppa011302m / 18597087 / 18599360 / 2274 / AT5G38900.1b / DSBA oxidoreductase family protein / 214 / 63,08 / 6E-77 / + / /
ppa012296m / 18597087 / 18599509 / 2423 / AT5G38900.1 / DSBA oxidoreductase family protein / 213 / 55,4 / 6E-63 / + / /
ppa005069m / 18600204 / 18604076 / 3873 / AT3G02300.1b / regulator of chromosome condensation (RCC1) family protein / 463 / 77,97 / 0 / + / /
ppa010249m / 18604427 / 18608655 / 4229 / AT1G69120.1 / AP1 (APETALA1); DNA binding / protein binding / protein heterodimerization/ transcription activator/ transcription factor / 152 / 72,37 / 1E-52 / - / -
ppa010548m / 18619188 / 18623759 / 4572 / AT3G02310.1 / SEP2 (SEPALLATA 2); DNA binding / protein binding / transcription factor / 251 / 74,9 / 1E-95 / - / -
ppa010577m / 18619188 / 18623759 / 4572 / AT3G02310.1b / SEP2 (SEPALLATA 2); DNA binding / protein binding / transcription factor / 250 / 75,2 / 5E-97 / - / -
ppa026503m / 18629652 / 18630269 / 618 / AT5G15802.1b / unknown protein / 110 / 56,36 / 9E-30 / - / -
ppa016385m / 18630946 / 18633249 / 2304 / AT3G30210.1b / MYB121 (MYB DOMAIN PROTEIN 121); DNA binding / transcription factor / 217 / 51,15 / 2E-52 / - / -
ppa013380m / 18636187 / 18636866 / 680 / N/A / N/A / N/A / N/A / N/A / N/A / N/A
ppa003386m / 18638891 / 18643879 / 4989 / AT3G02320.1b / RNA binding / tRNA (guanine-N2-)-methyltransferase / 558 / 78,85 / 0 / - / -
ppa007756m / 18645262 / 18647647 / 2386 / AT3G02230.1b / RGP1 (REVERSIBLY GLYCOSYLATED POLYPEPTIDE 1); cellulose synthase (UDP-forming) / 337 / 91,1 / 0 / + / /
ppa005994m / 18649075 / 18651638 / 2564 / AT3G30180.1b / BR6OX2 (BRASSINOSTEROID-6-OXIDASE 2); monooxygenase/ oxygen binding / 464 / 67,24 / 0 / + / Up PTG
ppa007503m / 18660657 / 18662638 / 1982 / AT1G13680.1b / phospholipase C/ phosphoric diester hydrolase / 348 / 62,64 / 1E-129 / - / -
ppa014104m / 18664030 / 18664766 / 737 / AT3G29970.1b / germination protein-related / 86 / 69,77 / 5E-34 / + / /
ppa024465m / 18666252 / 18668102 / 1851 / AT5G15630.1 / IRX6 / 397 / 80,1 / 0 / - / -
ppa005522m / 18668649 / 18671935 / 3287 / AT5G60920.1 / COB (COBRA) / 435 / 74,25 / 0 / - / -
ppb023073m / 18676574 / 18678411 / 1838 / AT3G02210.1 / COBL1 (COBRA-LIKE PROTEIN 1 PRECURSOR) / 180 / 42,22 / 1E-33 / - / -
ppb020721m / 18704742 / 18706234 / 1493 / AT1G56440.1 / serine/threonine protein phosphatase-related / 56 / 62,5 / 7E-16 / - / -
ppa022025m / 18716879 / 18718491 / 1613 / AT2G21660.2 / CCR2 (COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2); RNA binding / double-stranded DNA binding / single-stranded DNA binding / 55 / 65,45 / 5E-15 / - / -
ppa007463m / 18748304 / 18751578 / 3275 / AT5G15640.1b / mitochondrial substrate carrier family protein / 319 / 79,31 / 4E-147 / + / Up PTG
ppa010178m / 18748304 / 18749962 / 1659 / AT5G15640.1 / mitochondrial substrate carrier family protein / 210 / 80,95 / 2E-98 / + / Up PTG
ppa015454m / 18752057 / 18753454 / 1398 / AT4G01240.1 / unknown protein / 329 / 58,66 / 3E-111 / - / -
ppa011807m / 18754276 / 18755073 / 798 / AT3G02220.1b / unknown protein / 167 / 69,46 / 7E-63 / - / -
ppa013367m / 18755340 / 18756208 / 869 / AT1G24140.1a / Matrixin family protein / 92 / 32 / 7E-6 / + / Up PTG
ppa019352m / 18760652 / 18763276 / 2625 / AT3G51550.1 / FER (FERONIA); kinase/ protein kinase / 908 / 45,15 / 0 / - / -

a BLASTP TAIR matches with an E-value >1e-6 not included by IPGI

b Gene pairs that are ‘best-reciprocal BLASTP hits’ between Prunus and Arabidopsis

SUPPLEMENTARY REFERENCES

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