Supplemental Fig. 1. GO Cellular Component distribution of genes with expression changes in spaceflight.The annotation of Cellular Components for those Arabidopsis genes with highest similarity to the C. richardii genes in this study is presented. All genes with significant changes, both up and down-regulated during spaceflight are included in this graph.
Supplemental Fig. 2. GO Molecular Function distribution of genes with expression changes in spaceflight.The annotation of Molecular Functions for those Arabidopsis genes with highest similarity to the C. richardii genes in this study is presented. All genes with significant changes during spaceflight, both up and down-regulated, are included in this graph.
Supplemental Fig. 3. GO Biological Process distribution of genes with expression changes in spaceflight.The annotation of Biological Processes for those Arabidopsis genes with highest similarity to the C. richardii genes in this study is presented. All genes with significant changes during spaceflight, both up and down-regulated are included in this graph.
Supplemental Fig.4.Down regulation of C. richardii Transferase family protein TUG in microgravity. Gene expression changes as determined by microarray and Q RT-PCR analysis with two control genes, APT (adenine phosphoribosyltransferase) and TUB (alpha-tubulin). For each time point there is an independent comparison between ground control samples and shuttle flight samples. The lowest expression level, shuttle flight, is set to 1 and expression level of ground samples are relative comparison to 1. All fold changes are statistically different from 1 (shuttle flight) unless indicated by asterix (*). Separate statistical analyses of the Q RT-PCR and microarray data are provided in Supplemental tables 3 and 4.
Supplemental Fig.5.Down regulation of C. richardii Protodermal factor 1 TUG in microgravity. Gene expression changes as determined by microarray and Q RT-PCR analysis with two control genes, APT (adenine phosphoribosyltransferase) and TUB (alpha-tubulin). For each time point there is an independent comparison between ground control samples and shuttle flight samples. The lowest expression level, shuttle flight, is set to 1 and expression level of ground samples are relative comparison to 1. All fold changes are statistically different from 1 (shuttle flight) unless indicated by asterix (*). Separate statistical analyses of the Q RT-PCR and microarray data are provided in Supplemental tables 3 and 4.
Supplemental Fig.6.Up regulation of C. richardii Late embryogenesis abundant protein TUG in microgravity. Gene expression changes as determined by microarray and Q RT-PCR analysis with two control genes, APT (adenine phosphoribosyltransferase) and TUB (alpha-tubulin). For each time point there is an independent comparison between ground control samples and shuttle flight samples. The lowest expression level, ground, is set to 1 and expression level of shuttle flight samples are relative comparison to 1. All fold changes are statistically different from 1 (ground) unless indicated by asterix (*). Separate statistical analyses of the Q RT-PCR and microarray data are provided in Supplemental tables 3 and 4.
Supplemental Fig.7.Up regulation of C. richardii Cysteine proteinase TUG in microgravity. Gene expression changes as determined by microarray and Q RT-PCR analysis with two control genes, APT (adenine phosphoribosyltransferase) and TUB (alpha-tubulin). For each time point there is an independent comparison between ground control samples and shuttle flight samples. The lowest expression level, ground, is set to 1 and expression level of shuttle flight samples are relative comparison to 1. All fold changes are statistically different from 1 (ground) unless indicated by asterix (*).Separate statistical analyses of the Q RT-PCR and microarray data are provided in Supplemental tables 3 and 4.
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