MMP-1 Expression in Dermal Fibroblasts After Treatment with Different Combinations Of

Suppl Fig. 1

MMP-1 expression in dermal fibroblasts after treatment with different combinations of both Fcm and Fkcm

Linear regression model from densitometric analyses. The result shows that the change in the mixture composition (independent variable) accounted for almost 98% of the MMP-1 up-regulation (dependent variable). Model was developed from data of three independent experiments with a P value of 0.001

Suppl Fig. 2

Quantification of collagen content in adipofascial groin flaps

Masson’s trichrome staining in sections from adipofascial groin flaps. The blue color represents the collagen density. Data are representative of at least 10 HPF. Kruskal-Wallis test (nonparametric ANOVA) showed a significant different between groups (P value  0.0001). The Dunn’s multiple comparisons test showed that the relative level of collagen staining was significantly higher in sham and Bmc groups compared to that of normal tissue (*P 0.001). Bmc and sham groups did not exhibit differences in the collagen expression (NS). Flaps from rats treated with Bmc plus anti-TGF- type-II receptor neutralizing antibody (Bmc+TGF) exhibited a very significant lower expression of local collagen compared to that of sham (**P 0.05) and Bmc groups (*P 0.001). In addition, the Bmc+TGF group showed similar expression of collagen compared to that of normal tissue (NS)

Suppl Fig.3

Detection of PKH26 signals on stem cells and precursor cells from rats

(a)Bone marrow-derived stem cells and precursor cells (Bcm) were able to maintain the PKH26 staining (red) after 14 days of culture on plates. The right panel corresponds to the merge of images from immunofluorescence microscopy (PKH26/left panel) and phase contrast microscopy (Pcm/middle panel). Insert shows details of PKH26 staining of cells. (b) The left panel corresponds to the merge of positive PKH26 expression (red) and DAPI staining (blue) in Bcm injected within adipofascial groin flaps in rats during the endpoint (14 days after primary surgery). PKH26 signal was negative in sham flap and negative groin tissue. Scale bar: 100 m.