A)Serine proteases
1 R FT 121
CL LMUT0131C RYPKKKYFFRC-N-KNFTKWDEDIMLIRLNRPVRKSAHIEPLSLPSNPPSEDS
S35689 VIGGDECNINEHRFLVALYDGLSGTFLCGGTLINQEWVLTAQHCNRSLMNIYLGMHNKN-VKFDDEQRRYPKKKYFFRC-N-KNFTKWDED---IRLNRPVRFSAHIEPLSLPSNPPSEDS
V SS
LMUT0128C IVGGDECNINEHRFLVALYDPDG--FFCGGTLFNEEWVVTAAHCDRENMEIKLGMHSKK-VPNKDKQTRVAKEKFF--CLSSKNYTLWDKDIMLIRLDSPVNNSTHIAPVSLPSSPPSVGS
KN LMUT0366S VIGGDECNINEHRFLVALYDPDG--FSCGGTLINEEWMLTAAHCDRENMEIKLGMHSNK-VPNEDEQTRVPKEKFF--CLSNKNYTLWD
LV_Ka VVGGDECNINEHRSLVAIFVSTG--FFCSGTLINQE????????????????????????VLNQED???????KFI--CPNKKNNEVLD???????????VTNSENIVPLSLPSSPPSVGS
P81176 IIGGDECNINEHRFLVALYTPRSRTLFCGGTLINQEWVLTAAHCDRKNFRIKLGMHSKK-VPNKDEQTRVPKEKFF--CLSSKNYTLWDKDIMLIRLDSPVKNSTHIEPFSLPSSPPSVGS
PNR D
LMUT0402S VFGGDECNINEHRSLVVLFNSSG--FLCAGTLINKEWVLTAAHCDSENFQMQLGVHSKK-VPNKDEETRDPKEKFI--CPNRKKNDEKDKDIMLIRLNRPVSNSEHIALLSLPSSPPSVGS
PA LMUT0491S TDPVSNSEHIALLSLPSSPPSVGS
P84036 VFGGDECNINEHRSLVVLFNSNG--FLCAGTLINKEWVLTAAHCDSNLFQILVGVHSKKYVPNKDEETRKKDEKFI--CPNRA-YTLWDKDIMLIRLNRPVTNSEHIELLSLPSSPPSVGS
Q91516 VFGGDECNINEHRSLVVLFNSNG--FLCGGTLINQDWVVTAAHCDSNNFQLLFGVHSKK-ILNEDEQTRDPKEKFF--CPNRKKDDEVDKDIMLIKLDSSVSNSEHIAPLSLPSSPPSVGS
LMUT0755S RWDFDNQEWVLTAAHCDIKNFQIQLGVHSKK-VRNEDEQTRDPKEKFF--CLSSKTNDEWDKDIMLIRLDSPVSNSPHIAPLSLPSSPPSVGS
122 R 240
CL LMUT0131C VFFVMGWG-QITSPQETLPDVPHCANINLFNYTVCRGAYPRM--PTKGRVLCAGVLEGGIDTCNRDSGGPLICNGQFQGIVFWGPDPCAQ
S35689 VCRVMGWG-QITSPPETLPDVPHCANINLFNYTVCRGAYPRM--PTK--VLCAGVLEGGIDTCNRDSGGPLICNGQFQGIVFWGPDPCAQPDKPGVYTKVFDYLDWIQSVIAGN--TTCS-
LMUT0128C VCRIMGWG-RISPAKGTYPDVPHCVNINLLDYKMCRAIYPEFGLPATSRVLCAGILEGGKDTCKGDSGGPLICNGQFQGIASWGDDPCAQPNKPAAYTKVFDHLDWIQGIIAGNTNVNCPP
KN LMUT0366S
LV_Ka VCRIMGWG-TITPEKKTYPN-PHCANINLLDDAECHVGYERQ--GKEYRTLCAGVVQGGKDTCQGDSGGPLICNGQFQGI?????HPCGHP????????VFDYTEWIQSILAGNTAATCPP
P81176 VCRIMGWG-RISPTEETFPDVPHCVNINLLEYEMCRAPYPEFELPATSRTLCAGILEGGKDTCRGDSGGPLICNGQFQGIASWGDDPCAQPHKPAAYTKVFDHLDWIKSIIAGNTDASCPP
LMUT0402S VCRIMGWG-TISPTKEIYPDVPHCADINILDHAVCRAAYSGW--LATSTTLCAGILEGGKDSCHGDSGGPLICNGQFQGIVSLGRHPCGHPDEPGVYTKVFDYTDWIQSIIAGNTDAACPP
PA LMUT0491S VCRIMGWG-TISPTKEIYPDVPHCADINILDHAVCRAA
P84036 VCRIMGWGKTISPTKEIYPDVPHCADINILDHAVCRAIYPEFGLPATSRVLCAGILEGGKDSCH?????????????????????HPCGHPDGPGVYTKVFDYTDWIQSIIAGNTDAACPP
Q91516 VCRIMGWG-KTIPTKEIYPDVPHCANINILDHAVCRTAYSWR--QVANTTLCAGILQGGRDTCHFDSGGPLICNGIFQGIVSWGGHPCGQPGEPGVYTKVFDYLDWIKSIIAGNKDATCPP
LMUT0755S LCSIMGWG-TISSTKETYPNVPRCANINILDYAVCRAAYPWW--PVTTRKLCAGILEGGKDSCQGDSGGPLICNGQFQGIVSWGP
B)C-type Lectins
1 10 20 30 40 50 60 70 80
LMUT0074C AGCPSGWSSY----KGHCYKPFNEPKNWADAENFCTQQHTGGHLVSFHSSKEADFVVKLAFQTFGR---DIFWMGLSNVW
AAQ15155 ADCLSGWSSY----EGHCYKPFNELKNWADAENFCTQQQAGGHLVSFQSSEEADFVVKLAFQTFDH---SIFWMGLSNVW
LMUT0078C ADCPNGWYSY----EGHCYQIFHLFKTWDDAERFCREQAKGGHLVSIESAEEADFVADLVADNMKRF-IPYIWIGLRVQG
AAQ15153 ADCLSGWSSY----EGHCYKAFELYKTWEDAESFCMEGVKGGHLVSIESSGEADFVAQLISENMKRL-DFFVWIGLRVQG
AAB49518 NNCPQDWLPM----NGLCYKIFDEQKAWEDAEMFCRKYKPGCHLASFHRYGESLEIAEYISDYHKG--QAEVWIGLWDKK
LMUT0114C GFCASGWVQY----KSACYKVVRQRYTWTEAEISCQRYSPTSHLASIHSTEENNFIFHLMGKPLDYKQGQAYWIGAHDTF
BINS0004C TVCPSGTFGYKDGSEWYCYKFYEDQFTFHDAEEECQF-KWRGHLASLTKGKQVKSIAAYVTKENPE--GDLVWIGLREVK
AAU11827 SSCPKGWTHH----GSRCFTFHRGSMDWASAEAACIR--KGGNLASIHNRREQNFITHLIHKLSGE--NRRTWIGGNDAV
NP_114433 PSCAPGWFYH----KSNCYGYFRKLRNWSDAELECQSYGNGAHLASILSLKEASTIAEYISGYQRS---QPIWIGLHDPQ
81 90 100 110 120 130 140 150
LMUT0074C N---KCSWQWSNAAMLKYEAWAEES------YCVYFKSTNN--KWKSCACRMEAYFVCEFQA------
AAQ15155 N---QCNWQWSNAAMLRYKAWAEES------YCVYFKSTNN--KWRSRSCRMMANFVCEF------
LMUT0078C EQK-KCTSKWSDGSSVSYENWDESE------LKTCLGLEQDTNYEKWENVYCGKINPFVCEA------
AAQ15153 DEK-QCNSEWSDGSSVSYENWIESE------SKTCLGLEQQTKFRKWVNLYCGQRIPFVCEA------
AAB49518 K---DFSWEWTDRSCTDYLTWDKNQPDHYEGKEFCVELVSLTGYRLWNDQVCESKNAFLCQCKF------
LMUT0114C K---EGTFVWTDGSKFDFQSFPSEQPDGLTGEHYLGSWFLRNEKITWNDYNNSWKFGSVCKYSLANGGCSSSCEAK
BINS0004C GSSVKTRWRWTDGTRSMYKKWSYGEPKIGHYDDPCVGLSPASGYVEWVARECTNTFPFLCKWKPS------
AAU11827 K---EGMWFWSDGSKFNYKGWKKGQPDKHVPAEHCAETNFKGAF--WNNALCKVKRSFLCAKNL------
NP_114433 K---RQQWQWIDGAMYLYRSWSGKSMG---GNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP------
Supplemental figure 02. - Alignments of L. muta clusters of serine proteases and CTLs. A)Serine proteases were joined according to the groups proposed by Wanget al., 2001 (CL, coagulating enzymes; KN, kininogenases; PA, plasminogen activators). “?” are non sequenced venom segments. The typicalconserved residues found on L. muta clusters (LMUTs) are shown above each group. Representative sequences are referred to their Entrez-Genbank accession numbers, except for L. muta LV_Ka (Weinberget al. 2004). B) L. muta clusters of CTLs were aligned with representative molecules, referred to their accession numbers, except for B. insularis BINS0004C (Junqueira-de-Azevedo andHo 2002).