Table S1. Primers used in the qPCR analysis.

Bacterial groups / Name / Sequence / Tmp / Size / Reference
Total Bacteria / Forward / CGT GCC AGC AGC CGC GG / 60oC / 293 bp / 1
Reverse / TGG ACT ACC AGG GTA TCT AAT CCT G
Bifidobacterium genus / Forward / GAT TCT GGC TCA GGA TGA ACG C / 60oC / 232 bp / 1
Reverse / CTG ATA GGA CGC GAC CCC AT
Blautia coccoides group / Forward / AAA TGA CGG TAC CTG ACT AA / 53oC / 440 bp / 1
Reverse / CTT TGA GTT TCA TTC TTG CGA A
Bacteroides-Prevotella / Forward / GGTGTCGGCTTAAGTGCCAT / 68°C / 140 bp / 1
Reverse / CGGA(C/T)GTAAGGGCCGTGC
Lactobacillus spp. / Forward / AGCAGTAGGGAATCTTCCA / 58°C / 341 bp / 1
Reverse / CACCGCTACACATGGAG
Akkermansia muciniphila / Forward / CAG CAC GTG AAG GTG GGG AC / 60ºc / 327 bp / 1
Reverse / CCT TGC GGT TGG CTT CAG AT
Enterobacteriaceae family / Forward / CATTGACGTTACCCGCAGAAGAAGC / 63ºC / 195 bp / 2
Reverse / CTCTACGAGACTCAAGCTTGC
Faecalibacterium prausnitzii / Forward / CCA TGA ATT GCC TTC AAA ACT GTT / 60ºC / 141 bp / 3
Reverse / GAG CCT CAG CGT CAG TTG GT
Fusobacterium nucleatum / Forward / CAACCATTACTTTAACTCTACCATGTTCA / 60ºC / 111bp / 4
Reverse / GTTGACTTTACAGAAGGAGATTATGTAAAAATC
Archaea groups / Iniciadores / Sequence / Tmp / Size / Reference
Methanobacteriales / MBT857F / CGWAG GGAAG CTGTT AAGT / 54ºC / 343bp / 5
MBT929F-Tq / AGCAC CACAA CGCGT GGA
MBT1196R / TACCG TCGTC CACTC CTT
Methanomicrobiales / MMB282F / ATCGR TACGG GTTGT GGG / 63ºC / 506bp / 5
MMB749F-Tq / TYCGA CAGTG AGGRA CGAAA GCTG
MMB832R / CACCT AACGC RCATH GTTTA C
Methanosarcinaceae / Msc380F / GAAAC CGYGA TAAGG GGA / 50ºC / 408bp / 5
Msc492F-Tq / TTAGC AAGGG CCGGG CAA
Msc828R / TAGCG ARCAT CGTTT ACG
Methanosaetaceae / Mst702F / TAATC CTYGA RGGAC CACCA / 50ºC / 168bp / 5
Mst753F-Tq / ACGGC AAGGG ACGAA AGCTA GG
Mst862R / CCTAC GGCAC CRACM AC
Methanobrevibacter / MET-105f / TGGGAAACTGGGGATAATACTG / 55ºC / 282 bp / 6
MET-386r / AATGAAAAGCCATCCCGTTAAG
M. smithii / Mnif-342f / AACAGAAAACCCAGTGAAGAG / 55ºC / 222 bp / 6
Mnif-363r / ACGTAAAGGCACTGAAAAACC

References

  1. Collado MC, Isolauri E, Laitinen K, Salminen S. Distinct composition of gut microbiota during pregnancy in overweight and normal-weight women. Am J Clin Nutr 2008; 88, 894-899.
  2. Bartosch S, Fite A, Macfarlane GT, McMurdo ME. Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol 2004;70(6):3575-81.
  3. Sokol H, Seksik P, Furet JP, Firmesse O, Nion-Larmurier I, Beaugerie L, Cosnes J, Corthier G, Marteau P, Doré J. Low counts of Faecalibacterium prausnitzii in colitis microbiota. Inflamm Bowel Dis 2009;15(8):1183-9.
  4. Castellarin, M., Warren, R., Freeman, J., Dreolini, L., Krzywinski, M., Strauss, J. et al. Fusobacteriumnucleatum infection is prevalent in human colorectal carcinoma. Genome Res 2012; 22: 299–306.
  5. Yu Y, Lee C, Kim J, Hwang S. Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. Biotechnol Bioeng 2005; 89(6):670-9.
  6. Ufnar JA, Wang SY, Christiansen JM, Yampara-Iquise H, Carson CA, Ellender RD. Detection of the nifH gene of Methanobrevibacter smithii: a potential tool to identify sewage pollution in recreational waters. J Appl Microbiol 2006;101(1):44-52.