LOCUS Exported File 8969 bp ds-DNA circular SYN 04-DEC-2013
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pLVX-ZsGreen
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 8969)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Wednesday, Dec 04, 2013 from SnapGene 2.1.0
FEATURES Location/Qualifiers
source 1..8969
/organism="recombinant plasmid"
/mol_type="other DNA"
LTR 1..635
/label="5' LTR"
/note="5' LTR"
misc_binding 636..653
/label="PBS"
/note="PBS"
misc_feature 685..822
/label="packaging signal"
/note="packaging signal"
misc_binding 1303..1536
/label="RRE"
/note="RRE"
primer_bind 1866..2710
/note="seq.ZsGrev"
misc_feature 2028..2151
/label="cPPT/CTS"
/note="cPPT/CTS"
promoter 2205..2605
/gene="SFFV"
/label="SFFV"
/note="SFFV"
primer_bind 2468..3400
/note="seq.SFFVfw"
CDS 2645..3340
/codon_start=1
/label="ZsGreen"
/note="ZsGreen"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
primer_bind 3235..3507
/note="seq.ZsGfw"
misc_feature 3412..3539
/note="5' miR30 context"
misc_feature 3508..3539
/note="Illumina actual seq primer"
gap 3540..3561
/estimated_length="22"
misc_feature 3540..3561
/note="Mock Passenger"
misc_feature 3562..3580
/note="Loop"
gap 3581..3602
/estimated_length="22"
misc_feature 3581..3602
/note="Mock Guide"
misc_feature 3603..3735
/note="3' miR30 context"
primer_bind 3618..4170
/note="seq.ZsGfw"
promoter 3752..4256
/label="PGK Promoter"
/note="PGK Promoter"
primer_bind 4229..5224
/note="seq.PF"
CDS 4277..4876
/codon_start=1
/label="Puro(R)"
/note="Puro"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 4890..5481
/label="WPRE"
/note="WPRE"
LTR 5684..6320
/label="3' LTR"
/note="3' LTR"
rep_origin complement(6790..7463)
/direction=LEFT
/label="pUC origin"
/note="pUC origin"
CDS complement(7608..8604)
/codon_start=1
/label="Amp(R)"
/note="Amp"
/translation="DVRWHFSGKCARNPYLFIFLNTFKYVSAHETITLINASIILKKEE
YEYSTFPCRPYSLFCGILPSCFCSPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*EFS
PRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWHDSK
RIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKRRA*
HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**IWSR
*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*ALV"
ORIGIN
1 tggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccaca
61 cacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccac
121 tgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggcca
181 ataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccgg
241 agagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgag
301 agctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccg
361 ctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagat
421 cctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctga
481 gcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcct
541 tgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctc
601 agacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaag
661 cgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacgg
721 caagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctaga
781 aggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatggg
841 aaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatggg
901 caagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggct
961 gtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagat
1021 cattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagaca
1081 ccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagc
1141 aagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaag
1201 tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc
1261 aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgg
1321 gttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggc
1381 cagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc
1441 gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct
1501 ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaa
1561 actcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaaca
1621 gatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt
1681 aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt
1741 ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggta
1801 tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgt
1861 actttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct
1921 cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga
1981 cagagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaag
2041 gggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatac
2101 aaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacaggg
2161 acagcagagatccagtttatcgatcacgagactagcctcgatcgagctagctgcagtaac
2221 gccattttgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggc
2281 gggtacatgaaaatagctaacgttgggccaaacaggatatctgcggtgagcagtttcggc
2341 cccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaaga
2401 acagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgttt
2461 ccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcct
2521 gcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagctcacaaccc
2581 ctcactcggcgcgccagtcctccgacagactgagtcggccggtggatctaccggtggatc
2641 caccatggcccagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatgga
2701 gggctgcgtggacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaa
2761 gggcaagcaggccatcaacctgtgcgtggtggagggcggccccttgcccttcgccgagga
2821 catcttgtccgccgccttcatgtacggcaaccgcgtgttcaccgagtacccccaggacat
2881 cgtcgactacttcaagaactcctgccccgccggctacacctgggaccgctccttcctgtt
2941 cgaggacggcgccgtgtgcatctgcaacgccgacatcaccgtgagcgtggaggagaactg
3001 catgtaccacgagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaa
3061 gaagatgaccgacaactgggagccctcctgcgagaagatcatccccgtgcccaagcaggg
3121 catcttgaagggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctg
3181 ccagttcgacaccgtgtacaaggccaagtccgtgccccgcaagatgcccgactggcactt
3241 catccagcacaagctgacccgcgaggaccgcagcgacgccaagaaccagaagtggcacct
3301 gaccgagcacgccatcgcctccggctccgccttgccctgaagatcgcggccgcgactcta
3361 gaatttactagtcatatgataagatctctcgactagggataacagggtaattgtttgaat
3421 gaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggatta
3481 cttcttcaggttaacccaacagaaggctcgagaaggtatattgctgttgacagtgagcgn
3541 nnnnnnnnnnnnnnnnnnnnntagtgaagccacagatgtannnnnnnnnnnnnnnnnnnn
3601 nntgcctactgcctcggaattcaaggggctactttaggagcaattatcttgtttactaaa
3661 actgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaa
3721 ttaaatcacttttttcaattgacgcgtaattctaccgggtaggggaggcgcttttcccaa
3781 ggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcc
3841 tctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtg
3901 gccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagc
3961 tcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatgg
4021 acagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagcttt
4081 gctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggg
4141 gcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgc
4201 ttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacct
4261 gcagcccaagcttaccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacga
4321 cgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgcca
4381 caccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcac
4441 gcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggt
4501 ctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcat
4561 ggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgcc
4621 gcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccacca
4681 gggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccgg
4741 ggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcgg
4801 cttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccg
4861 caagcccggtgcctgaccgcgtctggaacaatcaacctctggattacaaaatttgtgaaa
4921 gattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaa
4981 tgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaat
5041 cctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgt
5101 gcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcc
5161 tttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcc
5221 ttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgg
5281 ggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcggga
5341 cgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgc
5401 tgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccc
5461 tttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagc
5521 aatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaaga
5581 ggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaa
5641 ggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagggctaattca
5701 ctcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccc
5761 tgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtg
5821 ctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagagaacac
5881 cagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttaga
5941 gtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagta
6001 cttcaagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagg
6061 gaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagc
6121 tgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctg
6181 gctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtag
6241 tgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcag
6301 tgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttg
6361 caaagaaatgaatatcagagagtgagaggccttgacattgctagcgttttaccgtcgacc
6421 tctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccg
6481 ctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaa
6541 tgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaac
6601 ctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt
6661 gggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcga
6721 gcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgca
6781 ggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttg
6841 ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagt
6901 cagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcc
6961 ctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctccct
7021 tcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtc
7081 gttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctta
7141 tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagca
7201 gccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaag
7261 tggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaag
7321 ccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt
7381 agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa
7441 gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggg
7501 attttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatga
7561 agttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgctta
7621 atcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactc
7681 cccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatg
7741 ataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgga
7801 agggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgt
7861 tgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatt
7921 gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcc
7981 caacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttc
8041 ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggca
8101 gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag
8161 tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcg
8221 tcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaa
8281 cgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa
8341 cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtga
8401 gcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttga
8461 atactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatg
8521 agcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
8581 ccccgaaaagtgccacctgacgtcgacggatcgggagatcaacttgtttattgcagctta
8641 taatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcact
8701 gcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactg
8761 gataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgc
8821 caattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcatttta
8881 aagaaattgtatttgttaaatatgtactacaaacttagtagtttttaaagaaattgtatt
8941 tgttaaatatgtactacaaacttagtagt
//