E. coli
BR8706 / =Stbl2 Δ(lac-proAB) Δ(araFGH) ΔaraEp PCP18-araE; araE under constitutive CP18 promoter / (1)
CVC1881 / BR8706 l::500bp upstream region of parA1 (2959004 - 2958505) fused to lacZ / This study
CVC1882 / BR8706 l::500bp upstream region of ompU (676380 - 675881) fused to lacZ / This study
XL1-Blue / Strain for cloning / Agilent Technologies
V. cholerae
CVC796 / El Tor N16961; StrR / (2)
CVC797 / CVC796 DparA1 (Same as LK626) / (3)
CVC1118 / N16961 hapR+
CVC1123 / CVC1118 DparB1::Zeo / (4)
CVC1124 / CVC1118 DparAB1::Zeo / (4)
Saccharomyces cerevisiae
AH109 / MATa trp1-901 leu2-3 112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1 TATA-lacZ / Clontech
EGY48 / MATa trp1 his3 ura3 lexAops-LEU2 / Clontech
Y187 / MATa ura3- 52 his3- 200 ade2- 101 trp1- 901 leu2- 3 112 gal4D, met– gal80D URA3::GAL1UAS -GAL1TATA -lacZ / Clontech
Plasmids
pBAD24 / PBAD cloning vector, pBRori; ApR / (4)
pBJH1 / pEG202 + parA1; ApR / (4)
pBJH3 / pKT25 + parA1; KmR / (4)
pBJH4 / pKT25 + parB1; KmR / (4)
pBJH5 / pUT18C + parA1; ApR / (4)
pBJH6 / pUT18C + parB1; ApR / (4)
pBJH7 / pUT18C + ompU (675880 - 674828); ApR / This study
pBJH9 / pKT25 + ompU (675880 - 674828); KmR / This study
pBJH15 / pDSW209 + parB1 (2957704 - 2956823); ApR / This study
pBJH17 / pMLB1109 + PparAB1 (2959004 - 2958503); ApR / This study
pBJH18 / pMLB1109 + PompU (676384 - 675881); ApR / This study
pBJH23 / pEG202 + parB1; ApR / (4)
pBJH34 / pMLB1109 + 500bp upstream region of VC0067 (68617 - 68118); ApR / This study
pBJH36 / pMLB1109 + 500bp upstream region of VC0076
(77135 - 76636); ApR / This study
pBJH37 / pMLB1109 + 500bp upstream region of VC0069 (68796 - 69295); ApR / This study
pBJH65 / A promoter cloning vector with a gfp reporter gene, pBRori; ApR / This study
pBJH89 / pBJH65 + parAB1 with promoter (2958605 - 2956823); ApR / This study
pBJH90 / pBJH65 + parAB1 (2958504 - 2956823); ApR / This study
pBJH91 / pBJH65 + parB1 (2957704 - 2956823); ApR / This study
pBJH105 / pGB2 + parS1-1 (62438 - 62423); SpR / This study
pBJH119 / pMLB1109 + 100 bp upstream region of ompU (675980 - 675881); ApR / This study
pBJH120 / pMLB1109 + 400 bp upstream region of ompU (676280 - 675881); ApR / This study
pBJH128 / pMLB1109 + 200 bp upstream region of ompU (676080 - 675881); ApR / This study
pBJH129 / pMLB1109 + 320 bp upstream region of ompU (676180 - 675881); ApR / This study
pBJH138 / pMLB1109 + 180 bp upstream region of ompU (676060 - 675881); ApR / This study
pBJH139 / pMLB1109 + 250 bp upstream region of ompU (676310 - 675881); ApR / This study
pBJH142 / pMLB1109 + 210 bp upstream region of ompU (676090 - 675881); ApR / This study
pBJH143 / pMLB1109 + 220 bp upstream region of ompU (676100 - 675881); ApR / This study
pBJH144 / pMLB1109 + 230 bp upstream region of ompU (676110 - 675881); ApR / This study
pBJH145 / pMLB1109 + 240 bp upstream region of ompU (676120 - 675881); ApR / This study
pBJH172 / pKT25 + toxRS (1048024 - 1046644); KmR / This study
pBJH173 / pUT18C + toxRS (1048024 - 1046644); ApR / This study
pDSW209 / A pTrc99A derived vector for generating IPTG inducible gfp fusions, pBRori; ApR / (5)
pEG202 / Yeast two-hybrid bait vector, pBRori, 2μ ori; HIS3, ApR / Clontech
pGADT7g / Yeast two-hybrid prey vector, pBRori, 2μ ori; LEU2, ApR / (6, 7)
pGBGT7g / Yeast two-hybrid bait vector, pBRori, 2μ ori; TRP1, GenR / (8)
pGB2 / A pSC101 derived vector; SpR / (9)
pJG4-5 / Yeast two-hybrid prey vector, pUCori, 2μ ori; TRP1, ApR / Clontech
pMLB1109 / Promoter cloning vector with the lacZ reporter gene, pBRori; ApR / M.L. Berman
pRKG212 / pBAD24 + parB1; ApR / (4)
pKT25 / Bacterial two-hybrid bait vector, p15Aori; KmR / Euromedex
pRN005 / pBAD24 + parAB1; ApR / (4)
pRN006 / pBAD24 + parA1; ApR / (4)
pSH18-34 / Yeast two-hybrid reporter vector, pBRori, 2μ ori; URA3, ApR / Clontech
pUT18C / Bacterial two-hybrid prey vector, pUCori; ApR / Euromedex
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