Supplemental materials
Table S1 Primers used for Real time RT-PCR.
Gene / Forward primer / Reverse primerTMMa / GATTGTGTGATGCAGAAACGTC / TCTTCTTCTCTAGATAGGTGCTGGA
RD29Ab / ATCACTTGGCTCCACTGTTGTTC / ACAAAACACACATAAACATCCAAAGT
MYB2b / TGCTCGTTGGAACCACATCG / ACCACCTATTGCCCCAAAGAGA
ABI3b / TCCATTAGACAGCAGTCAAGGTTT / GGTGTCAAAGAACTCGTTGCTATC
ABI4b / GGGCAGGAACAAGGAGGAAGTG / ACGGCGGTGGATGAGTTATTGAT
ABI5b / CAATAAGAGAGGGATAGCGAACGAG / CGTCCATTGCTGTCTCCTCCA
PP2Cc / TGGAGATCCGGAGGTTTAAG / TCTCCTCCGCCTCTGTAAGT
GL1d / CAATGGAACCGCATCGTCAG / TGATGAACAATGACGGTGGA
GL3d / CGCAGGAGAAAGAACATCAG / CGAGGATTGAACCGAATGAG
KISe / GGATGATGATTCCGGATTGC / TTCCAATTCCGCCAAAGTG
ZWIe / TCCTCCTCTTCTCTCGCTCTGT / ATTAGCCGCAGAAAAGCTTCAA
ACTINf / GGTAACATTGTGCTCAGTGGTGG / AACGACCTTAATCTTCATGCTGC
a. Delgado D, Ballesteros I, Torres-Contreras J, Mena M, Fenoll C (2012) Dynamic analysis of epidermal cell divisions identifies specific roles for COP10 in Arabidopsis stomatal lineage development. Planta 236:447-461
b. Shen YY, Wang XF, Wu FQ, Du SY, Cao Z, Shang Y, Wang XL, Peng CC, Yu XC, Zhu SY, Fan RC, Xu YH, Zhang DP (2006) The Mg-chelatase H subunit is an abscisic acid receptor. Nature 443:823-826
c. Mosher S, Moeder W, Nishimura N, Jikumaru Y, Joo SH, Urquhart W, Klessig DF, Kim SK, Nambara E, Yoshioka K (2010) The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner. Plant Physiol 152:1901-1913
d. Gao Y, Gong X, Cao W, Zhao J, Fu L, Wang X, Schumaker KS, Guo Y (2008) SAD2 in Arabidopsis functions in trichome initiation through mediating GL3 function and regulating GL1, TTG1 and GL2 expression. Journal of Integrative Plant Biology 50:906-917
e. designed in this study.
f.Wang Z, Meng P, Zhang X, Ren D, Yang S (2011) BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis. Plant J 67:1081-1093
Table S2 Stomatal density and index in overexpression lines
Genotypes / Stomatal density / Stomatal indexWT / 169.75±5.78d / 0.26±0.00c
S4 / 155.35 ±6.47d / 0.25±0.01c
WT(vector) / 176.95±5.82d / 0.25±0.01c
TMM-OX lines / T2-1 / 162.55±5.62d / 0.28±0.01c
T2-2 / 175.93±4.80d / 0.26±0.00c
T2-3 / 170.78±5.78d / 0.27±0.00c
tmm-1 / 559.67±28.98a / 0.52±0.01a
tmm mutants / tmm-4 / 473.25 ±15.81b / 0.54±0.01a
tmm-5 / 581.28±26.16a / 0.52±0.01a
tmm-6 / 355.97±9.25c / 0.40±0.01b
tmm-OX lines / 1300-tmm4 / 190.33±9.17d / 0.29±0.01c
1300-tmm5 / 184.16±6.29d / 0.26±0.01c
1300-tmm6 / 189.30±7.33d / 0.27±0.01c
Data, including S.E., were obtained from at least five 2-week-old primary leaves. “T2-1”represented “one line of secondgeneration lines from wild type transformed with1300-TMM construct” and so on.The superscript letters represent statistical significance at p<0.05 (Duncan test), and values with the same letter are not statistically different.
Fig. S1 Intermediate stomatal phenotype appeared in F1 line of tmm-1and tmm-6. a Stomatal density, stomatal index, density of stomatal clusters. bPercent of each cluster size. Bars represented S.E, n=30. Data, including S.E., were obtained from at least five 20-day-old true leaves.
Fig.S2Complementation of the tmm mutant phenotype. Stomatal density of tmm alleles transformed with TMMpro:: TMM-GFPor 1300-TMM.“tmm-4 (T2-1)”represented “one line of the secondgeneration of transgenic lines”. Bars represented S.E, n=30. Data, including S.E., were obtained from at least five 2-week-old primary leaves
1