Supplemental Table 1. Yeast strains Used in This Study
Strain / Genotype / ReferenceNOY1051 / MATa rpa135D::LEU2 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 fob1D::HIS3 pNOY117 (CEN, RPA135, TRP1), (143 rDNA repeats) / French et al., 2003
NOY886 / MATa rpa135D::LEU2 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 fob1D::HIS3 pNOY117 (CEN, RPA135, TRP1), (42 rDNA repeats) / French et al., 2003
JS772 / MATa ura3D0 his3D0 leu2D0 (derivative of S288C) / Winzeler et al., 1999
MWK028 / MATa trp1-1 ura2-52 can1-100 leu2-3,112 his3-11,15 ade2-1 met2-D1 lys2-D2 LEU2::snr6 pYCp50 (URA3, CEN4, ARS1, SNR6) / Prescott et al., 2004
YPH499 / MATa ura3-52 lys2-801amber ade2-101ocher trp1-D63 his3-D200 leu2-D1 / Sikorski and Hieter, 1989
YPH259 / MATa ura3-52 lys2-801amber ade2-101ocher his3-D200 leu2-D1 / Sikorski and Hieter, 1989
mpp10::HIS3 pGAL1::MPP10 / As YPH499 but mpp10D::HIS3 pGAL1::MPP10 (GAL10/GAL1::MPP10 ADE2 URA3 CEN6 ARSH4) / Dunbar et al., 1997
mpp10-5 / As YPH499 but mpp10-5::HIS3 (This mpp10 truncation deletes the last 95 carboxy-terminal amino acids.) / Lee and Baserga, 1999
FDY18 / As YPH499 but GAL1::U14. U14 is under galactose control and snR190 is deleted from the SNP190-U14 dicistron. / this work
GAL::RRP2 / As YPH499 but GAL1::RRP2 / this work
GAL1::UTP7 / As YPH499 but GAL1::HA3 -Utp7-kanMX4 / Dragon et al., 2002
pGAL1::IMP3 / As YPH259 but imp3D::HIS3 pGAL1::IMP3 (GAL10/GAL1::IMP3 ADE2 URA3 CEN6 ARSH4) / Lee and Baserga, 1999
JH84 / MATa leu2-3,12 ura3-52 his3-D ade2-1can1-100 u3aD UASGAL::U3A::URA3 U3B::LEU2 / Samarsky and Fournier, 1998
pGAL1::RPF2 / ura3-52 lys2-801amber ade2-101ocher his3-D200 leu2-D1 rpf2D::HIS3 pGAL1::RPF2 (GAL10/GAL1::RPF2 ADE2 URA3 CEN6 ARSH4) / Wehner and Baserga, 2002
YTT166 / RAD5 derivative of W303-1A (MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100); same as W1588-4C / Tsukiyama et al., 1999
References for Supplemental Table S1
Prescott, E.M., Osheim, Y.N., Jones, H.S., Alen, C.M., Roan, J.G., Reeder, R.H., Beyer, A.L. and Proudfoot, N.J. (2004). Proc. Natl. Acad. Sci. USA 101, 6068-6073.
Samarsky, D.A. and Fournier, M.J. (1998). Functional mapping of the U3 small nucleolar RNA from the yeast Saccharomyces cerevisiae. Mol. Cell Biol. 18, 3431-3444.
Sikorski, R.S. and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
Tsukiyama, T., Palmer, J., Landel, C.C., Shiloach, J., Wu, C. (1999). Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev. 13, 686-697.
Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J.D., Bussey, H., Chu, A.M., Connelly, C., Davis, K., Dietrich, F., Dow, S.W., El Bakkoury, M., Foury, F., Friend, S.H., Gentalen, E., Giaever, G., Hegemann, J.H., Jones, T., Laub, M., Liao, H., Davis, R.W., et al. (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906.