Supplementary Table 1 . Sequences of Morpholinos Used

Supplementary Table 1. Sequences of morpholinos used.

Targeted gene / Sequence
Random Sequence / CCT CTT ACC TCA GTT ACA ATT TAT A
scl / GCG GCG TTA CCT GTT AAT AGT GGC G
aldh1a2 / GTT CAA CTT CAC TGG AGG TCA TCG C
aldh1a3 / TAT AGT CCC GTT CTG TGC CAT AGC A
aldh2a / CGC AGC ATT TTC TGC TCC CTC GCC T
aldh2b / GCG CAG CAT TTT CCA CTC ACT CGC C
aldh3a2 / TCG AGA CAT CGT TAG GAG TCT GTC T
aldh3d1 / CAG CAC CTG CTT CTG GCC CTC CAT T
aldh4a1 / CAG ACC TCG CTC GCA GCA TCG TTG A
aldh5a1 / CGC TGC AAC TGC ACC ATG CTC GGT A
aldh6a1 / TGG AAC GCA CTA ATA TCG CTG CCA T
aldh7a1 / TTC GGA CAC TCG GCA ACA GTT TAT G
aldh8a1 / ATA TTT CAT GTC TTT TGA CAT CGC A
aldh9a1a / TTT TTG ACG GGA ACA CAG ATT TCG A
aldh9a1b / GCA GGA GAC ACC TCA TAA GGG CCA T
aldh16a1 / TCG TGC TGG ATG GTC CAT ACT CCA T
aldh18a1 / GAG AGA CAT GGT GAT CTG ATC TTA A

Supplementary Table 2. Sequences of primers used in cloning zebrafish aldh isoforms for RNA probes.

Primer name / Sequence
aldh1a2F / AAA CCC AAC CGG CAT CTT CA
aldh1a2R / CCA GCT GCA ACG AAC ATG TA
aldh1a3F / TTC ATT GCT ATG GCA CAG AAC GGG
aldh1a3R / AAA CAG AGT GAC GGT GAA GCG AGA
aldh2aF / AAG GCG AGG GAG CAG AAA AT
aldh2aR / TGC TGT TCA CAT GAA GTC GG
aldh2bF / TGA AGG CGA GTG AGT GGA AA
aldh2bR / ACA TTC ATG AAG CGT CCT CC
aldh3a2F / ACA GAC CAG ACA GAC TCC TA
aldh3a2R / GTT TGT CTC CAC TTG TCA CC
aldh3d1F / TTC ACA ATG GAG GGC CAG AA
aldh3d1R / TTT TGT TCC CTG GGC AAG TG
aldh4a1F / CGG TTT TGG GAC GGT AAG AA
aldh4a1R / CTG TAC GGC CAA ATC AAC GA
aldh6a1F / CAT GGC AGC GAT ATT AGT GC
aldh6a1R / CCT GTA GAT GGA TGG TGG AA
aldh7a1F / AAC CGC AGC ACC GAA TAT GT
aldh7a1R / CCA CCA CTA ACT AGG CAA CA
aldh8a1F / CTG AGT GCA GCG CAA AAC TT
aldh8a1R / CGC TTC ATC CAT GTC AGT GT
aldh9a1aF / AAA CAC CAT GGC TCA GCA AC
aldh9a1aR / CTT AAA ACA GGC TCT CCA CG
aldh9a1bF / TTA GGC TGA AGA TGG CCC TT
aldh9a1bR / CTC AAC TCT AGC TGG TGG TT
aldh16a1F / CTG TGC ACG ACA TCT TTC AG
aldh16a1R / GTG CTT TCT CAT GCA GTT GG
aldh18a1F / TCA GAT CAC CAT GTC TCT CC
aldh18a1R / CCG ACC AAA TGT GAG TGT TG

Supplementary Table 3. Sequences of primers used in cloning GFP-fusion expressing vectors for checking morpholino efficiency.

Primer name / Sequence
aldh1a2gfpF / AAA CCC AAC CGG CAT CTT CA
aldh1a2gfpR / TCT GTC CAC ACA CTC CAA TG
aldh1a3gfpF / ACA GCA CTT CTT CTT CTT CTT CTT
aldh1a3gfpR / GCA TGG TTT TCC CAT GAA TC
aldh2agfpF / AGT TCA AGT GCT GAA GGC GA
aldh2agfpR / TGG GCT CAT GTC TTG TGT AGC A
aldh2bgfpF / TAG GAA GAG CGC ATT TCT GC
aldh2bgfpR / TCA TGC ATT TGA CCA CCA TG
aldh3a2gfpF / TCT CAG CGT TCA GCT CAT TG
aldh3a2gfpR / GCT CCA TAA CGC TGG CTG T
aldh3d1gfpF / CTG AAT GAA AGG CGA AAA CC
aldh3d1gfpR / CAG CAA TTG CAC CAA TCA AA
aldh4a1gfpF / TGG TTC CTC AAC AGG AGG AT
aldh4a1gfpR / CAGF TCT TAC CCT GTC CGA TCA
aldh6a1gfpF / CGC ACA TTA GGG GGT GAA TA
aldh6a1gfpR / GCA GCC GGT ATG TGT ATG TG
aldh7a1gfpF / TGC GCG ACT TTT TAC TCA GA
aldh7a1gfpR / CAT GAC CTG GCC TTT CAG AT
aldh8a1gfpF / CTG AGT GCA GCG CAA AAC TT
aldh8a1gfpR / GAT CCA TCT GAC TGC AGT CA
aldh9a1agfpF / AGA ACA GAA GCA GGC TCT GT
aldh9a1agfpR / CTC CCA AAG GCT CTC TTC TGG
aldh9a1bgfpF / GTT CAG TTT TAG GCT GAA GAT GG
aldh9a1bgfpR / GGT GCG TGT ACG CAA ATG AA
aldh16a1gfpF / CTG TGC ACG ACA TCT TTC AG
aldh16a1gfpR / GAG CTG GCA GAA CTT TTA GC
aldh18a1gfpF / GTT GAT GTG GCA TCG AAG AA
aldh18a1gfpR / GAG CCA TAA GGC CAC TCT GT

Supplementary Table 4. Sequences of primers used in Q-PCR.

Primer name / Sequence
β-actinQF / TTC CTT CCT GGG TAT GGA ATC
β-actinQR / GCA CTG TGT TGG CAT ACA GG
sclQF / CTA TTA ACC GTG GTT TTG CTG G
sclQR / CCA TCG TTG ATT TCA ACC TCA T
lmo2QF / GGA CGC AGG CTT TAC TAC AAA C
lmo2QR / CCG GAT CCT CTT TTC ACA GGA A
gata1QF / AAG ATG GGA CAG GCC ACT AC
gata1QR / TGC TGA CAA TCA GCC TCT TTT
α-ehbQF / TGC TCT CTC CAG GAT GTT GA
α-ehbQR / TCA CAG TCT TGC CGT GTT TC
β-ehbQF / AGG CTC TGG CAA GGT GTC TCA
β-ehbQR / CAT TGG GTT TCC CAG GAT
spi1QF / GGG CAG TTT TAA CCA AAG ATC A
spi1QR / CCC AAG AGT GAT CGT TCT GAC
l-plastinQF / GAA GCT CTG ATC GCT CTG CT
l-plastinQR / GCT TCT TTT CAT CCG TCA GG
c-mybQF / TTT CTA CCG AAT CGA ACA GAT G
c-mybQR / CAA TCA CCC GTT GGT CTT CT
runx1QF / CGT CTT CAC AAA CCC TCC TC
runx1QR / TAC TGC TTC ATC CGG CTT CT
pax2aQF / CCA AGG AGT CAG ACC TTG TGA
pax2aQR / CCC CCA ATA ACT CCA GGT TT
egr2aQF / GCT CGG ACG AAC TTA CCA GA
egr2aQR / GAA TGT GGG TCG TCA GGT G