Supplementary Fig.ANucleotide sequence and deduced amino acid sequence of EgT2RIP. The predicted N-terminal signal peptide and linker peptide are underlined. The position of the putative translation termination codon is indicated by an asterisk.
2 AGA GTT CCT AAA TTT CCT CTC AAG TTG CTG ATG AAA GGA AAT ATG 46
1 M K G N M 5
47 AAA GTG TGG AAA GCA GTG GCA ACA TGG GTT TGG TGG GCT GCC ATA 91
6 K V W K A V A T W V W W A A I 20
92 GTT GGA CCA GCT TGG GTG TGC ACG TCA ACG GTA GAA GAT AAA TAC 136
21 V G P A W V C T S T V E D K Y 35
137 ACA CTC CGC TAC AAC ACG GTG CGC TTC ACC ACC GTA AAT GCC ACT 181
36 T L R Y N T V R F T T V N A T 50
182 AGG GAA AGC TAC ACG CAG TTT ATG AAA GAT CTG AGA GAT CAA TTG 226
51 R E S Y T Q F M K D L R D Q L 65
227 GCA AGT GGA GAT GAG AGG TAT GGA ATA CCA GTG TTA CGT GAT CCA 271
66 A S G D E R Y G I P V L R D P 80
272 TCC ACA GTG CCC GAT TCC CAG CGG TTT CTT TTG GTG GAA CTC TCA 316
81 S T V P D S Q R F L L V E L S 95
317 AAT TGG GGG GAG GCA TCC GTC ACA TTA GCA GTA GAT GTC ATC AAT 361
96 N W G E A S V T L A V D V I N 110
362 GCA TAT GTG GTG GCT TAT CAG GCG GGA GAT CAG TCC TAC TTC TTT 406
111 A Y V V A Y Q A G D Q S Y F F 125
407 AGA GAT GCT CCG GAC GTT GCA TTT TCT AAT CTT TTC ACA AAC ACA 451
126 R D A P D V A F S N L F T N T 140
452 GAG CGA CAA ACT CTT TCA TTC AAT AGT AGC TAT CCC GCA CTT CAA 496
141 E R Q T L S F N S S Y P A L Q 155
497 AGA GTT GCA GGC GAA GAT AGA GAG AAC ATC GAC CTA GGA ATA TTG 541
156 R V A G E D R E N I D L G I L 170
542 GCA CTA GAG GAA GCG ATC TCA TCG CTT CAT AGG ACC AGC AGC GTT 586
171 A L E E A I S S L H R T S S V 185
587 CAG CAA AAC ACC CAG GCT CGT TCT CTT ATA GTT TCC ATC CAG ATG 631
186 Q Q N T Q A R S L I V S I Q M 200
632 GTT TCA GAG GCA GCG CGA TTT AGA TAT ATC GAG CAA CGG GTG CGC 676
201 V S E A A R F R Y I E Q R V R 215
677 CAA AGC ATT ACA AGC GGA GGA TAC CAA ACC TTT CGC CCC GAC GCT 721
216 Q S I T S G G Y Q T F R P D A 230
722 AGC ATG CTC AGC CTC GAG AAT AAC TGG GGA GCC CTC TCC ACT GCA 766
231 S M L S L E N N W G A L S T A 245
767 ATC CAG GAA TCC AAT CAA GGA GTT TTT TGC AGC CCA GTT CAA TTA 811
246 I Q E S N Q G V F C S P V Q L 260
812 CAA AGA CCC AAC TAT ACC CCG GTC ATG GTG GAC AGT GTG ACG CCA 856
261 Q R P N Y T P V M V D S V T P 275
857 ATC ATA ATT TCA AAC CTG GCA TTC ATG GTG TTC GTT TGC GCG AAC 901
276 I I I S N L A F M V F V C A N 290
902 CAA GCA TCA TCT CAG TTT TCT CCG CTT ATA AGG TCC GTC GTC GCC 946
291 Q A S S Q F S P L I R S V V A 305
947 GAG GAT GAC GGC GAT ACT TGT GCA CAT CCA GAG CCC ACG ACA CGC 991
306 E D D G D T C A H P E P T T R 320
992 ATC AGC GGT CGA AAT GGT CTA TGC GTT GAT GTG AGG GAT GGA CAA 1036
321 I S G R N G L C V D V R D G Q 325
1037 TAC AAC GAC GGA AAC CCG ATA CAG CTG TGG CCG TGT AAA TCT AAT 1081
336 Y N D G N P I Q L W P C K S N 350
1082 ACG GAC GCG AAT CAA TTG TGG ACT TTG AAA AGG GAT GGG ACA ATT 1126
351 T D A N Q L W T L K R D G T I 365
1127 CGA TCT AAT GGC AAG TGC TTG ACC ACC TAT GGT TAT AGT CCA GGA 1171
366 R S N G K C L T T Y G Y S P G 380
1172 AGC TAT GTG ATG ATC TAT GAT TGC ACC ACT GCC GTG ACT GAT GCC 1216
381 S Y V M I Y D C T T A V T D A 395
1217 ACC CGT TGG GAA GTA TGG GAC AAT GGA ACC ATC ATA AAT CCC AGG 1261
396 T R W E V W D N G T I I N P R 410
1262 TCA GCG CTA GTC TTG AGC GCA GAG TCA GGG AAT AGT GGC ACC ACA 1306
411 S A L V L S A E S G N S G T T 425
1307 CTC ACC GTG GAG ACC AAC ATC TAT GCT TCC CGT CAA GGC TGG CTC 1351
426 L T V E T N I Y A S R Q G W L 440
1352 GCC AGC AAT AAT ACA CAA CCT TTC GTG GCG TCC ATT GTT GGG TTT 1396
441 A S N N T Q P F V A S I V G F 455
1397 ATG GAT CTC TGT TTG GAA ACG GAC GGA ACC GGC GTG TGG GTA GGG 1441
456 M D L C L E T D G T G V W V G 470
1442 AAT TGT GCG AGC AAC AAG GTA GAA CAA AAA TGG GCT CTC TAT GCA 1486
471 N C A S N K V E Q K W A L Y A 485
1487 GAT GGT TCT ATA CGG CCT CAG CAG AAC CAA GAC CGC TGC CTC ACC 1531
486 D G S I R P Q Q N Q D R C L T 500
1532 AGT AAT GAT CTT CTC CAG GGA ACG AAT ATC ACC GTT GTC TCT TGT 1576
501 S N D L L Q G T N I T V V S C 515
1577 AGT CCT GGG TCC TCT GGG CAA CGC TGG GTG TTT ACA AAC GAT GGC 1621
516 S P G S S G Q R W V F T N D G 530
1622 ACC ATT TTG AAT TTG AAG CAG GGA CTC GTG ATG GAC GTG AAA GGA 1666
531 T I L N L K Q G L V M D V K G 545
1667 TCT GAT CCA AGC CTT AAG CAA ATA ATC TTG TGG CCG TTC ACT GGG 1711
546 S D P S L K Q I I L W P F T G 560
1712 AAT CCT AAC CAG AAG TGG CTT CCA ATG CTT TGA TTA CAT CTG CTC 1756
561 N P N Q K W L P M L * 570
1757 TCT TGC GGT GTC TCC AGC TTA TGA GTA GCT CAA ATA AAA GAC ATC 1801
1802 AAA TTA TGG GTG AGG TTA GCG AGC CCT TGG TAG TTT GTG TGG TGG 1846
1847 CCC TGG CCC ACT TGG TTG TTC GAA TTT CCC TTT TCC TTA GGT TTG 1891
1892 TTT CCT ATC TTC TCT TAA TAT GTG TGG TTG TGG AGT TTG GTC TGT 1936
1937 TAA GTG GAG TGA ATA GGT AGC AAC TTC AGA GCT ACG AGC CCA ACA 1981
1982 TGC ATG CAA GGA GCG TCT GTT ATT CCC TAT ATA TGC TGT ATT TAA 2026
2027 ATG GGG ATA AAT TGC CTC CGT TGT TAC TGA ATT TGG TCT GTT AAG 2071
2072 TGG AGT GAA TAG GTA GCA ACT TCA GAG CTA CGA GCC CAA CAT GCA 2116
2117 TGC AAG GAG CGT CTG TTA TTC CCT ATA TAT GCT GTA TTT AAA TGG 2161
2162 GGA TAA ATT GCC TCC GTT GTT ACT GAA 2188
Cloning and Expression of Oil Palm (Elaeisguineensis Jacq.) Type 2 Ribosome Inactivating Protein in Escherichia coli
International Journal of Peptide Research and Therapeutics
Yung-Chie Tan · Wan-Yong Ho · NoorjahanBanuAlitheen · Wong Mui Yun · Chai-Ling Ho
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
E-mail: