OIE RL for «White Spot Disease » – « Grace Chu-Fang Lo» – «China Taipei»

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / White spot disease
Address of laboratory / Institute of Zoology, National TaiwanUniversity,No.1, Sec, Roosevelt Road, Taipei 106, Taiwan
Tel.: / (886-2) 2363-3562
Fax: / (886-2) 2363-8179
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Prof/Dr Grace Chu-Fang Lo
Name(including Title and Position) of OIE Reference Expert: / Prof/Dr Grace Chu-Fang Lo
Date of submission to the OIE / Feb 20, 2012

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

EachToR(blueitalicised text)has been placed as a heading covering the group of questions related to it.

Please note the reditalicisedtext is given as guidance and should be deleted from your report and substitute with your data. Examples are based onpast Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test recommended by the OIE / Total number of test performed last year
Indirect diagnostic tests / Nationally / Internationally

Western blot assay

/ 125 test / 0
Direct diagnostic tests / Nationally / Internationally

PCR

/ 340 / 30

TEM

/ 30 / 0

Real-time RT-PCR gene vp28

/ 1207 / 0

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagentsofficially recognised by the OIE or other international bodies?

Yes No

3.Did your laboratory supply standard reference reagentsto OIE Member Countries?

Yes No

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

YesNo

5.Did your laboratory produce vaccines?

Yes No

6.Did your laboratory supply vaccines to OIE Member Countries?

Yes No

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

YesNo

8.Didyour laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

Yes No

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

YesNo

Name of OIE Member Country seeking assistance / Date
(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Vietnam / 20 May / 5

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes No

Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was provided
Regional Animal Health Office No.6, Vietnam / Monitoring the WSSV DNA in shrimp feed / remote assistance

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

YesNo

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Didyour Laboratory collect epizootiologicaldata relevant to international disease control?

 YesNo

13.Didyour laboratory disseminate epizootiologicaldata that had been processed and analysed?

YesNo

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a)Articles published in peer-reviewed journals:...... 5

b)International conferences:...... 4

c)National conferences:...... 0

d)Other:...... website

a) Articles published in peer-reviewed journals:

  1. Leu, J.H., Lin, S.J., Huang, J.Y., Chen, T.C., Lo, C.F. (2012). A model for apoptotic interaction between white spot syndrome virus and shrimp. Fish and Shellfish Immunology. (Article in Press)
  2. Lin, S. J., Hsia, H. L., Liu W. J., Huang, J. Y., Liu, K. F., Chen, W. Y., Yeh, Y. C., Huang, Y. T., Lo, C. F., Kou, G. H.* and Wang, H. C. (2012). Spawning stress triggers WSSV replication in brooders via the activation of shrimp STAT. Developmental and Comparative Immunology, 38(1), 128-135.
  3. Chang, Y. S.,Liu, W. J., Chen, T. C., Chan, T. Y., Liu, K. F., Chuang, J. C., Kou, G. H., Lo, C. F. and Wang, H. C. (2012) Feeding hermit crabs to shrimp broodstock increases their risk of WSSV infection.Dis Aquat Organ. 98(3)193-199.
  4. Huang, J. Y., Liu, W. J., Wang, KC H.C., Lee, D. Y., Leu, J. H., Wang, H. C., Tsai, M. H., Kang, S. T., Chen, I. T., Kou, G. H., Chang, G. D.* and Lo, C. F. (2012)Penaeusmonodonthioredoxin restores the DNA binding activity of oxidized WSSV IE1. Antioxidants & Redox Signaling. 17(6)914-26.
  5. Chang, L.K., Huang, P.H., Shen, W.T., Yang, S.H., Liu, W.J., Lo, C. F.* (2012) Role of PenaeusmonodonKruppel-like factor (PmKLF) in infection by white spot syndrome virus. Dev. Comp. Immunol. 36 (1) 121-129.

b) International conferences：

  1. Lo, C. F. (2012). Integrative study of the interaction between white spot syndrome virus and its shrimp host MECO-TECO JOINT R&D WORKSHOP. Pasay City, Philippines. 17-18 December 2012.
  2. Lo, C. F. (2012). Systematic interactions between a large DNA virus (WSSV) and an invertebrate host (shrimp). The 8th Symposium of World's Chineses Scientist's on Crustacean Aquaculture. Ningbo, China. November 2012.
  3. Lo, C. F. (2012).Spawning stress triggers WSSV replication in brooders via the activation of shrimp STAT. The 12th Congress of the International Society of Developmental and Comparative Immunology (ISDCI). Fukuoka, Japan. July 8-13, 2012.
  4. Lo, C. F. (2012).Shrimp thioredoxin increases WSSV's pathogenicity by rescuing WSSV IE1's DNA binding ability under oxidative conditions. The 9th Asia-Pacific Marine Biotechnology Conference (APMBC). Kochi, Japan. July 14-16,2012.

c) Website:

Core Facilities for Shrimp Functional Genomics (SFGC):

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

Yes No

a)Technical visits:...... 1

b)Seminars:...... 0

c)Hands-on training courses:...... 0

d)Internships (>1 month):...... 1

Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding country
a / Thailand / 1
d / Thailand / 1

ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16.Does your laboratory have a Quality Management System certified according to an International Standard?

YesNo

17.Is your laboratory accredited by an international accreditation body?

YesNo

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

YesNo

ToR:To organise and participate in scientific meetings on behalf of the OIE

19.Did your laboratory organise scientific meetings on behalf of the OIE?

Yes No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

Yes No

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

YesNo

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

YesNo

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes No

ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24.Did your laboratory organiseor participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

Yes No

ToR:To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

 Yes No

Kind of consultancy / Location / Subject (facultative )
The OIE ad hocmeetings on Aquatic Animal Health Surveillance / Paris / Drafting WSD surveillance chapter
Responding the member country comments onAquatic Manual / Taipei / Responding Comments to Chapter 2.2.6(WSD) OIE AquaticManual
Amending and updating the Chapter 2.2.6(WSD) OIE Aquatic Manual

Annual reports of OIE Reference Laboratories, 20121