Journal: PNAS India

A comparison of the bacterial diversity of two shallow freshwater lakes in China

Table S1 Data (Lake Nanhu)

Parameter
(mg/ml) / 1991 [1] / 1994 [1] / 1995 [1] / 1996 [1] / 1997 [1] / 1998 [1] / 2003 [1] / 2010
NH4+ / - / 0.164 / 0.089 / 0.375 / 0.020 / 0.310 / 2.738 / 0.942
NO3- / - / 0.103 / 0.562 / 0.176 / 0.161 / 0.350 / 2.080 / 2.981
NO2- / - / 0.049 / 0.052 / 0.139 / 0.064 / 0.002 / 0.171 / 1.496
TN / 1.500 / 1.990 / 1.198 / 1.940 / 1.220 / 3.010 / 6.266 / 6.710
TP / 0.029 / 0.019 / 0.025 / 0.108 / 0.146 / 0.298 / 0.683 / 0.543
DOC / 8.87 / 9.90 / 7.30 / 9.48 / 9.67 / 9.80 / 5.80 / 116.0

DOC: dissolvedorganic carbon; TN: total nitrogen; TP: total phosphorus

Table S2Data (Lake Niushan)

Parameter / 2006 [2] / 2007 [3] / 2010
TN (mg/L) / 0.698 / 0.804 / 0.873
TP (mg/L) / 0.026 / 0.033 / 0.004
Chl a (μg/L) / - / 2.62 / 3.67

Chl a: chlorophyll a; TN: total nitrogen; TP: total phosphorus

References

1.Wang Y, Xiongbang X, Yang X, Ma X, Chen C, Hu H(2005) The current situation of Lake Nanhu, Wuhan City and suggestions on ecological restoration. Reservoir Fisheries 25:65-66

2.Ye S, Li Z, Lek-Ang S, Feng G, Lek S, Cao W(2006) Community structure of small fishes in a shallow macrophytic lake (Niushan Lake) along the middle reach of the Yangtze River, China. Aquat Living Resour19:349–359

3.Cao X, Song C, Zhou Y (2010) Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes. J Appl Phycol 22:33-41

Table S3 16S rRNA gene clones of cyanobacteria obtained from Lake Nanhu

Sequence code / GenBank acc. no. / Nearest valid relative / Similarity
Nan 41 / JN941794 / Microcystis aeruginosa(AJ635430) / 99%
Nan 42 / JN941795 / Microcystis aeruginosa(AJ635430) / 99%
Nan 43 / JN941796 / Microcystis aeruginosa(AJ635430) / 99%
Nan 44 / JN941797 / Microcystis aeruginosa(AJ635430) / 99%
Nan 45 / JN941798 / Microcystis aeruginosa(AJ635430) / 99%
Nan 46 / JN941799 / Microcystis aeruginosa(AJ635430) / 99%
Nan 47 / JN941800 / Microcystis aeruginosa(AJ635430) / 99%
Nan 48 / JN941801 / Microcystis sp. (AJ133170) / 99%
Nan 49 / JN941802 / Microcystis sp. (AJ133170) / 99%
Nan 50 / JN941803 / Microcystis sp. (AJ133170) / 99%
Nan 51 / JN941804 / Microcystis sp. (AJ133175) / 99%
Nan 52 / JN941805 / Microcystis sp. (AJ133175) / 99%
Nan 53 / JN941806 / Microcystis aeruginosa(AJ635430) / 99%
Nan 54 / JN941807 / Microcystis aeruginosa(AJ635430) / 99%
Nan 55 / JN941808 / Microcystis aeruginosa(AJ635430) / 99%
Nan 56 / JN941809 / Microcystis aeruginosa(AJ635430) / 99%
Nan 57 / JN941810 / Microcystis aeruginosa(AJ635430) / 99%
Nan 58 / JN941811 / Microcystis aeruginosa(AJ635430) / 99%
Nan 59 / JN941812 / Microcystis aeruginosa(AJ635430) / 99%
Nan 60 / JN941813 / Microcystis aeruginosa(AJ635430) / 99%
Nan 61 / JN941814 / Microcystis aeruginosa(AJ635430) / 99%
Nan 62 / JN941815 / Microcystis aeruginosa(AJ635430) / 99%
Nan 63 / JN941816 / Microcystis aeruginosa(AJ635430) / 99%
Nan 64 / JN941817 / Microcystis aeruginosa(AJ635430) / 99%
Nan 65 / JN941818 / Microcystis aeruginosa(AJ635430) / 99%
Nan 66 / JN941819 / Microcystis aeruginosa(AJ635430) / 99%
Nan 69 / JN941820 / Oscillatoriales cyanobacterium(HQ912972) / 98%
Nan 70 / JN941821 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 71 / JN941822 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 72 / JN941823 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 73 / JN941824 / Oscillatoriales cyanobacterium(HQ912973) / 100%
Nan 74 / JN941825 / Oscillatoriales cyanobacterium(HQ912973) / 99%
Nan 75 / JN941826 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 76 / JN941827 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 77 / JN941828 / Oscillatoriales cyanobacterium(HQ912972) / 100%
Nan 78 / JN941829 / Oscillatoriales cyanobacterium(HQ912972) / 99%
Nan 79 / JN941830 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 80 / JN941831 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 81 / JN941832 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 82 / JN941833 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 83 / JN941834 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 84 / JN941835 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 85 / JN941836 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 86 / JN941837 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 87 / JN941838 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 88 / JN941839 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 89 / JN941840 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 90 / JN941841 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 91 / JN941842 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 92 / JN941843 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 93 / JN941844 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 94 / JN941845 / Planktothrix pseudagardhii (FM177501) / 99%
Nan 107 / JN941846 / Planktothrix agardhii (GQ351571) / 100%
Nan 108 / JN941847 / Planktothrix agardhii (GQ351571) / 99%
Nan 111 / JN941848 / Oscillatoriales cyanobacterium(HQ912973) / 91%
Nan 112 / JN941849 / Phormidium autumnale(EF654084) / 91%
Nan 113 / JN941850 / Phormidium autumnale(EF654084) / 91%
Nan 114 / JN941851 / Phormidium autumnale(EF654084) / 91%
Nan 115 / JN941852 / Phormidium autumnale(EF654084) / 91%
Nan 116 / JN941853 / Phormidium autumnale(EF654084) / 91%
Nan 117 / JN941854 / Phormidium autumnale(EF654084) / 91%
Nan 118 / JN941855 / Phormidium autumnale(EF654084) / 91%
Nan 119 / JN941856 / Phormidium autumnale(EF654084) / 91%
Nan 120 / JN941857 / Phormidium autumnale(EF654084) / 91%
Nan 121 / JN941858 / Phormidium autumnale(EF654084) / 91%
Nan 122 / JN941859 / Phormidium autumnale(EF654084) / 91%
Nan 123 / JN941860 / Phormidium autumnale(EF654084) / 91%
Nan 124 / JN941861 / Phormidium autumnale(EF654084) / 91%
Nan 127 / JN941862 / Oscillatoriales cyanobacterium(HQ912973) / 92%

Table S4 16S rRNA gene clones of cyanobacteria obtained from Lake Niushan

Sequence code / GenBank acc. no. / Nearest valid relative / Similarity
Niu 72 / JN941952 / Microcystis aeruginosa(HE975020) / 99%
Niu 73 / JN941953 / Oscillatoria sancta(AF132933) / 99%
Niu 74 / JN941954 / Synechococcus sp. (HQ730085) / 99%
Niu 75 / JN941955 / Synechococcussp. (HQ730085) / 99%
Niu 76 / JN941956 / Cyanobium sp. (AY183115) / 99%
Niu 77 / JN941957 / Synechococcus sp.(AF330249) / 99%
Niu 78 / JN941958 / Cyanobium sp.(AM710363) / 99%
Niu 79 / JN941959 / Leptolyngbya sp. (FM177494) / 96%
Niu 80 / JN941960 / Leptolyngbya sp. (EF088337) / 99%
Niu 81 / JN941961 / Romeria sp. (JQ819251) / 99%
Niu 82 / JN941962 / Romeria sp.(JQ819251) / 98%
Niu 83 / JN941963 / Romeria sp.(JQ819251) / 98%