Supplemental Instruction
Iowa State University / Leader: / Brandon
Course: / Biol 212
Instructor: / Dr. Coffman/Manz
Date: / 4/30/2017
Biotechnology and Genomics (Ch. 20 & 21)
- What are the six steps of PCR?
- What are the steps of dideoxy sequencing?
- What are the steps of shotgun sequencing?
- How genes are identified within bacterial genomes?
- Why it is more difficultto identify genes in eukaryotes?
Energetics (Ch. 8 & 9)
- Given the following graphs, apply the following labels accordingly to the correct graph: Exergonic, Endergonic, Spontaneous, Non spontaneous, Energetically favorable, Energetically unfavorable.
- How are reaction rates affected by changes in reactant concentration and changes in temperature?
- Given the following reactions:
X + B → XB + H20 (ΔG = +17.7 kcal/mol)
Z + H20 → Y + B (ΔG = -9.5 kcal/mol)
Predict whether the following coupled reaction will be energetically favorable or energetically unfavorable.
X + Z → Y + XB
- How does competitive inhibition affect enzyme function?
- How does allosteric activation affect enzyme function?
- How does allosteric inhibition affect enzyme function?
- Describe the four steps of the cellular respiration process in words.
- How does an enzyme with both an active site and an inhibitory allosteric site that bind the same molecule regulate steps in the glycolysis pathway?
- What are the steps for processing pyruvate to acetyl CoA?
Photosynthesis (Ch. 10)
- Given the two reactions below:
CO2 + H2O + light energy →→→ (CH2O)n + O2
CO2 + H2S + light energy →→→ (CH2O)n + S
What could we expect the products of the subsequent reactions to look like?
14CO2 + H218O + light energy →→→
14C18O2 + H2O + light energy →→→
- What is the role of the light capturing reactions?
- If a specific color can be seen, this means that the wavelength of light responsible for that color is being what?
- If we are given a light spectrum that depicts yellow, orange, and red light being reflected, where would we find the majority of oxygen seeking bacteria on the light spectrum?
- What is the relationship between the absorption spectrum and the action spectrum?
- What is the relationship between light-dependent reactions and carbon fixation?
Cell Signaling (Ch. 11)
- What are two functions of the plant cell wall?
- What are two functions of the animal cell extracellular matrix?
- What is the role of tight junctions?
- What is the role of gap junctions?
- If conducting a cell mixing experiment where epidermis, mesoderm, and neural plate are placed into the same petri dish, what can we expect the outcome of this experiment to look like if we know that neural plate has a greater cell adhesion to the epidermis?