Supplies for Micro Lab (Magrann, Thurs Eve)

Supplies for Micro Lab (Magrann, Thurs eve)

Lab 1 / Ch 1 (Microscope use)
Letter E slides
Bacteria slides
Blood slides
Slides that have large colorful tissues (epidermis, liver, etc)
Immersion oil
Ch 3 (Microbes in the environment)
18 NA plates
8 Sterile test tubes of nutrient broth
Sterile swabs
Sterile pipettes
Sharpie pens (8)
Green tape (8 rolls)
Glo Germ Experiment
4 GloGerm kits
Ultraviolet light
Additional Hand Washing experiment
16 Mitis salivarius agar plates (2 per group)
Sterile cotton swabs
Lab 2 / Ch 4, 5, 6, 7, 9
24 empty, non-sterile tubes with caps for students to practice aseptic opening of tubes
8 NA broths
8 NA slants
8 Pure cultures of L. lactis
8 Pure cultures of P. aeuriginosum
8 Pure cultures of B. subtilis
8 Pure cultures of S. epidermidis
8 Pure cultures of Bacillis megatarium
8 Pure cultures of E. coli
methylene blue
Nigrosin stain
Endospore stain supplies for Bacillis megatarium
Water squirt bottles
Toothpicks
Disposable pipettes
Two racks for tubes in biohazard bucket
Two racks for tubes to go into incubator
Blotting paper
Immersion oil
8 small rulers
Sharpie pens (8)
Green tape (8 rolls)
Two racks for tubes in biohazard bucket
Two racks for tubes to go into the refrigerator
Lab 3 / Ch 11 (streak for isolation) and Ch 12 (Isolation media)
24 NA plates
8 MSA plates
8 EMB plates
9 Pure cultures of E. coli (one tube should be 10 ml for dilution technique)
8 Pure cultures of Pseudomonas aeruginosa
8 Pure cultures of Micrococcus luteus
8 Pure cultures of Staphylococcus epidermidis
8 sterile test tubes
Disposable plastic pipettes
Ch 11 (Dilution Series and Pour Plate Techniques
1 ml pipette or small (10 ml) graduated cylinder)
3 empty sterile Petri plates
1-2 bottles of melted agar for the plates
150 ml flasks (3 of them)
100 ml graduated cylinder
Disposable plastic pipettes
Spectrophotometer and supplies
8 Tube Racks
NOTE: We do not need the mixed culture tubes
NOTE: 5 plates for each lab group will go into refrigerator
Lab 4 / Ch. 24 (Disinfectants) and 25 (Antimicrobial drugs)
8 Pure cultures of Pseudomonas aeruginosa
8 Pure cultures of Staphylococcus aureus
8 Pure cultures of E. coli
8 tubes of diluted Lysol
8 tubes of diluted Pine-sol
8 tubes of contact lens cleaning solution
16 NA plates
24 NA slants to finish the Ch 11 experiment from last time
24 Mueller-Hinton agar plates
antibiotic discs
4 pairs of forceps
UV machine for enumeration of bacterial grown last lab
NOTE: The 24 NA slants need to stay at room temperature
Lab 5 / Ch 26 (Hand scrubbing experiment)
16 NA plates
8 scrub brushes
Waterless hand cleanser
Ch 45 (Skin bacteria)
24 MSA plates
Sterile cotton swabs
Novabiocin antibiotic discs (24 of them)
Forceps to handle the antibiotic discs
Ch. 46 (Respiratory bacteria)
Sterile cotton swabs
24 blood agar plates for individual throat swabs
1 blood agar plate for the class to see the control organisms
1 Pure culture of Streptococcus pyogenes
1 Pure culture of Streptococcus pneumoniae
Green tape (8 rolls)
Lab 6 / Ch 45 (continued from last week)
API Staph strip, mineral oil, bottle of liquid media to make a suspension from a colony from the MSA plate from last week or provide a pure culture of a Gram positive organism
Ch 36 (Protozoa)
Disposable pipettes
Live protozoa cultures:
Amoeba
Paramecium
Euglena
Protozoa slides
Giardia
Trypanosoma
Plasmodium
Motility observation of the protozoa
Depression slides
ProtoSlow
Ch 19 (Oxygen requirements)
9 Thioglycolate broths (plus some for soil project)
Ch 14 (Fermentation)
20 MRVP broths (2 for each group (plus some for soil project)
10 non-sterile test tubes (no caps needed)
8 Simmons Citrate agar slants (plus some for soil project
Motility Test (MIO tube is purple)
8 motility slants
Depression slides
Controls: 1 Pure culture of each of the following
Write the organism NAME on the tube
1) E. coli
2) Enterobacter aerogenes
3) Pseudomonas aeruginosa
4) Proteus vulgaris
Oxidase test filter paper and reagent, enough for soil project
Unknown Organism Projects
8 MacConkey’s agar plates
8 test tube racks
27 TSB tubes
8 tubes labeled with a number only:
Tubes #1 and 5 are Pseudomonas aeruginosa
Tubes 2 and 6 are Proteus vulgaris
Tubes 3 and 7 are Enterobacter aerogenes
Tubes 4 and 8 are E. coli
Schedule Retrogen to come next Weds to pick up the soil PCR samples for RNA analysis
Lab 7A
Tues lab 5:30-7 pm / PCR equipment for soil project
Ch 14 (Fermentation):
8 Glucose fermentation tubes (plus some for soil projects)
8 Lactose fermentation tubes (plus some for soil projects)
8 Sucrose fermentation tubes (plus some for soil projects)
1 Rack of the students’ “Unknown Organism” tubes (8 tubes total)
Green Tape (8 rolls)
NOTE: After 24 hours (on Weds night), the lab tech needs to remove these tubes from the incubator, and put the tubes that turned RED in a separate rack from the tubes that stayed YELLOW, then put both rack in the refrigerator.
Lab 7B / Reagents for Staph API kit
Sheets to record results of Staph API kit
Electrophoresis equipment
Ch 13 (Carbohydrate Catabolism)
V-P reagents 1 and 2
Methyl Red
Parafilm squares
10 Starch agar plates
20 OF-glucose deeps
Sterile Mineral oil
Pure cultures (one each)
E. coli
Pseudomonas aeruginosa
Ch 15 (Protein Catabolism)
9 Urea tubes
9 Gelatin tubes
Pure cultures (1 each)
Pseudomonas aeruginosa
Ch 16 (Protein Catabolism)
10 Phenylalanine slants
10 SIM media slants
Pure cultures (1 each)
E. coli
Enterobacter aerogenes
8 arginine, lysine, and ornithine decarboxylase tubes
8 Sharpie pens
8 rolls of Green tape
Ch 51 (Rapid ID methods)
4 Enterotubes and instruction booklet
1 Pure culture of each of the following:
E. coli
Pseudomonas aeruginosa
Enterobacter aerogenes
Ch 37 (Invertebrates)
Preserved parasitic worm slides (p.278 lab manual)
Trichinella spiralis
Taenia
Echinococcus
Enterobius vermicularis
Lab 8 / Finish Ch 16 (Protein catabolism)
10% Ferric chloride reagent for Phe slants
Kovac’s reagent for SIM slants and Enterotubes
Iodine for starch agar (enough to flood 8 Petri dishes)
Instructions for Enterotubes, and record sheets for them
Toothpicks to poke hole in indole well of Enterotube for Kovak’s reagent
Ice in a bucket for gelatin tubes
Ch 44 (Eliza technique)
Eliza kit for HIV, including reagents and instruction book
Flat shelf (slide tray?) to put the tiny plastic wells on in the incubator
Disposable SMALL plastic pipettes
8 plastic tubes (15 ml) with caps to hold PBS solution; place in a rack
8 small beakers (150 ml) for waste
Antibody 1, Antibody 2, HIV: put each of these in small plastic tube, labeled on top. Make 1 set for each lab group.
Prepare substrates 1 and 2 at 6:45 pm, put each of these in small plastic tube
Small green racks for each lab group
One plastic well for each lab group
Sharpie pens
Lab 9 / Lab Discussion Forum, no supplies needed
Lab 10 / Presentations of lab projects and Final lab exam, no supplies needed