Phosphonoformic acid trisodium hexahydrate 250mg irritant Irritant by ingestion, inhalation and skin contact. Irritating to eyes, respiratory system and skin. R36/37/38S26

5 INTENDED USE**

Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Dr. C. Sweet / Assessment Number* / 2082
Signature
Assessor / Katarina Baluch / Date of Assessment / 14/11/02

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / Laboratory S105
2 / PERSONS WHO MAY BE AT RISK
List names where possible / All members of Dr. C. Sweets group present and future; currently Ms Katarina Baluch, Dr. M. Kirby, Ms K.. Ball and Ms Tasneem Habib, Dr. S. J. Morris, Ms. I. Y. Pappworth and future M.Res. or 3rd year project students.
3 / ACTIVITY ASSESSED / Use of phosphoformic acid trisodium hexahydrate as a DNA synthesis inhibitor
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Phosphonoformic acid trisodium hexahydrate / 250mg / irritant / Irritant by ingestion, inhalation and skin contact. Irritating to eyes, respiratory system and skin. / R36/37/38
S26
5 / INTENDED USE**
Give brief details and attach protocol/instructions
Phosphonoformic acid trisodium hexahydrate is purchased as solid (250mg) and it will be diluted in 1.25ml water in the class 2 safety cabinet and stored in original container at 4°C. It will be used as a tissue culture medium supplement on cell monolayer in order to inhibit DNA synthesis. Phosphonoformic acid trisodium hexahydrate will be diluted to 200mg/ml in tissue culture medium in the class 2 safety cabinet.
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
ACUTE:
May be harmful by inhalation, ingestion, or skin absorption. Causes eye and skin irritation.
To the best of the manufacturer’s knowledge (Sigma-Aldrich) the chemical, physical and toxicological properties have not been thoroughly investigated.
TOXICITY DATA: Intravenous mouse LD50 510mg/kg
Intravenous rat LD50 500mg/kg
Incompatibilities: strong oxidising agents
Hazardous combustion or decomposition products – toxic fumes of carbon monoxide and carbon dioxide
Termal decomposition may produce toxic fumes of phosphorus oxides
Appearance and odor: white crystals
May cause congenital malformation in the foetus
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Main risk is from direct skin contact, ingestion and inhalation. Material is irritating to mucous membranes and upper respiratory tract.
Risk level is negligible if good chemical practise is followed. Concentrations used are well below that of the stated LD50.
Gloves, protective clothing and eye protection should be worn when handling the material.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Wear appropriate protective clothing including gloves when handling the material. Make dilutions in class 2 safety cabinet. Observe good chemical practice.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
None required
10 / MONITORING
Performance of control measures,
None required
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
The doses used can be washed down the sink.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
Review in 5 year (2007) unless greater concentrations to be used
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Fire fighting: Use carbon dioxide extinguisher. Wear breathing apparatus as emits toxic fumes under fire conditions.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Safety shower and eye bath. Wash contaminated clothing before reuse. Wash thoroughly after handling.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
If spill of solid, place a bag for incineration. Liquids may be mopped up with suitable absorbent material and incinerated. Ventilate area.
CONTACT PH ONE

10.10.00

* Prefix T is used for Teaching Assessment Number.

** Please include amount of chemicals used and how.

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.