Supplemental Table S1. Collection of analyzed krill samples off the Antarctic Peninsula
Sample Number1 / Date Collected / Lat (decimal) / Long (decimal)702k / 3-Feb-94 / -65.850139 / -71.378301
760m / 14-Jan-94 / -64.861083 / -67.694859
813m / 26-Jan-94 / -65.279413 / -64.270181
841m / 1-Feb-94 / -65.750845 / -68.592332
980334 / 16-Dec-97 / -64.783333 / -64.093
1Only samples used in final analysis shown
Supplemental Table S2. Primers for long PCR.
Primer Pairs / Reference / SequenceEus-cox1F / Shen et al. 2010 / GGT GCA TGA GCT GGA ATA GT
Eus-cox3R / Shen et al. 2010 / GCT GGC TGA AAA GTG ACA AC
Eus-cox3F / Shen et al. 2010 / GCA CAC GGA TTT CAC ACA TA
Eus-nad5R / Shen et al. 2010 / AGG TTG AGA TGG GTT AGG GT
Nad5-2F / Johansson, unpublished / GAG CTG CTA TTG CTG CAG GT
Cob1R / Johansson, unpublished / AGG GTT GGC AGG AAT GAA GT
Cob2F / Johansson, unpublished / GCG TGG TGA AAC TTT GGT TC
ES444R / Johansson, unpublished / GCA TCC GCG GTT ATA CTA AGG
Eus-rRNAF / Shen et al. 2010 / TAA GAA TGA GAG CGA CGG G
Eus-cox1R / Shen et al. 2010 / TTA AGT TGT GCA CCG TGA AG
Supplemental Table S3. Profile of mitochondrial features of E. superba sample 841M
Accession Number: JQ286348% A/T: / 68.02 / % Unknown Bases: / 0.35 / Size: / 13312 bp
Position / Intergenic Nucleotides
Feature / Size / From / To / Strand / % A/T
cox1a / 1476 / 1 / 1476 / + / 68.02
trnL-uagb / 66 / 1490 / 1555 / + / 13 / 62.12
cox2 / 688 / 1556 / 2243 / + / 0 / 63.95
trnK-uuu / 69 / 2244 / 2312 / + / 0 / 63.77
trnD-guc / 68 / 2313 / 2380 / + / 0 / 75.00
atp8 / 159 / 2381 / 2539 / + / 0 / 76.10
atp6 / 675 / 2533 / 3207 / + / -7 / 65.93
cox3 / 793 / 3207 / 3999 / + / -1 / 63.30
trnG-ucc / 67 / 4000 / 4066 / + / 0 / 67.16
nad3 / 354 / 4067 / 4420 / + / 0 / 63.84
trnA-ugc / 66 / 4420 / 4485 / + / -1 / 66.67
trnR-ucg / 67 / 4489 / 4555 / + / 3 / 61.19
trnN-guu / 65 / 4556 / 4620 / + / 0 / 73.85
trnS-ucu / 68 / 4621 / 4688 / + / 0 / 63.24
trnE-uuc / 69 / 4690 / 4758 / + / 1 / 72.46
trnF-gaa / 68 / 4858 / 4925 / - / 99 / 73.53
nad5 / 1731 / 4925 / 6655 / - / -1 / 69.27
trnH-gug / 66 / 6656 / 6721 / - / 0 / 69.70
nad4 / 1338 / 6722 / 8059 / - / 0 / 68.39
nad4L / 300 / 8053 / 8352 / - / -7 / 67.00
trnT-ugu / 66 / 8355 / 8420 / + / 2 / 74.24
trnP-ugg / 67 / 8422 / 8488 / - / 1 / 71.64
nad6 / 522 / 8492 / 9013 / + / 3 / 72.61
cob / 1137 / 9013 / 10149 / + / -1 / 65.88
trnS-uga / 71 / 10170 / 10240 / - / 20 / 67.61
nad1 / 939 / 10258 / 11196 / - / 17 / 69.86
trnL-uaa / 66 / 11213 / 11278 / - / 16 / 60.61
rrnL / 1308 / 11297 / 12604 / - / 18 / 75.00
trnV-uag / 72 / 12605 / 12676 / - / 0 / 72.22
rrnS / 631 / 12682 / 13312 / - / 5 / 73.22
aBeginning of cox1 and end of rrnS failed to be sequenced.Actual size of these regions may not be significantly longer than shown here.
btRNA names are followed by their anticodon sequence
Supplemental Table S4. Profile of mitochondrial features of E. superba sample 980349
Accession Number: JQ286349% A/T: / 68.09 / % Unknown Bases: / 0.24 / Size: / 13325 bp
Position / Intergenic Nucleotides
Feature / Size / From / To / Strand / % A/T
cox1a / 1485 / 2b / 1485 / + / 61.37
trnL-uagc / 66 / 1502 / 1567 / + / 16 / 62.12
cox2 / 688 / 1568 / 2255 / + / 0 / 73.22
trnK-uuu / 69 / 2256 / 2324 / + / 0 / 63.77
trnD-guc / 68 / 2325 / 2392 / + / 0 / 75.00
atp8 / 159 / 2393 / 2551 / + / 0 / 76.10
atp6 / 675 / 2545 / 3219 / + / -7 / 66.07
cox3 / 793 / 3219 / 4011 / + / -1 / 62.80
trnG-ucc / 67 / 4012 / 4078 / + / 0 / 67.16
nad3 / 354 / 4079 / 4432 / + / 0 / 63.84
trnA-ugc / 66 / 4433 / 4498 / + / 0 / 66.67
trnR-ucg / 67 / 4500 / 4566 / + / 1 / 61.19
trnN-guu / 65 / 4567 / 4631 / + / 0 / 73.85
trnS-ucu / 68 / 4632 / 4699 / + / 0 / 63.24
trnE-uuc / 69 / 4701 / 4769 / + / 1 / 72.46
trnF-gaa / 68 / 4869 / 4936 / - / 99 / 73.53
nad5 / 1731 / 4936 / 6666 / - / -1 / 69.21
trnH-gug / 66 / 6667 / 6732 / - / 0 / 69.70
nad4 / 1338 / 6733 / 8070 / - / 0 / 68.46
nad4L / 300 / 8064 / 8363 / - / -7 / 67.67
trnT-ugu / 66 / 8366 / 8431 / + / 2 / 74.24
trnP-ugg / 67 / 8433 / 8499 / - / 1 / 71.64
nad6 / 522 / 8503 / 9024 / + / 3 / 72.61
cob / 1137 / 9024 / 10160 / + / -1 / 66.23
trnS-uga / 71 / 10181 / 10251 / - / 20 / 67.61
nad1 / 939 / 10269 / 11207 / - / 17 / 70.18
trnL-uaa / 66 / 11224 / 11289 / - / 16 / 60.61
rrnL / 1308 / 11308 / 12615 / - / 18 / 75.46
trnV-uag / 72 / 12616 / 12687 / - / 0 / 72.22
rrnS / 633 / 12693 / 13325 / - / 5 / 73.14
aBeginning of cox1 and end of rrnS failed to be sequenced.Actual size of these regions may not be significantly longer than shown here.
bStart position of 2 used to accurately set the reading frame
CtRNA names are followed by their anticodon sequence
Supplemental Table S5. Profile of mitochondrial features of E. superba sample 702K
Accession Number: JQ286350% A/T: / 67.5 / % Unknown Bases: / 1.31 / Size: / 13310 bp
Position / Intergenic Nucleotides
Feature / Size / From / To / Strand / % A/T
cox1a / 1473 / 1 / 1476 / + / 60.83
trnL-uagb / 66 / 1490 / 1555 / + / 16 / 62.12
cox2 / 688 / 1556 / 2243 / + / 0 / 63.95
trnK-uuu / 69 / 2244 / 2312 / + / 0 / 63.77
trnD-guc / 68 / 2313 / 2380 / + / 0 / 75.00
atp8 / 159 / 2381 / 2539 / + / 0 / 75.47
atp6 / 675 / 2533 / 3207 / + / -7 / 65.63
cox3 / 793 / 3207 / 3999 / + / -1 / 63.18
trnG-ucc / 67 / 4000 / 4066 / + / 0 / 67.16
nad3 / 354 / 4067 / 4420 / + / 0 / 64.12
trnA-ugc / 66 / 4420 / 4485 / + / 0 / 66.67
trnR-ucg / 67 / 4487 / 4553 / + / 1 / 61.19
trnN-guu / 65 / 4554 / 4618 / + / 0 / 73.85
trnS-ucu / 68 / 4619 / 4686 / + / 0 / 63.24
trnE-uuc / 69 / 4688 / 4756 / + / 1 / 72.46
trnF-gaa / 68 / 4856 / 4923 / - / 99 / 73.53
nad5 / 1731 / 4923 / 6653 / - / -1 / 68.28
trnH-gug / 66 / 6654 / 6719 / - / 0 / 69.70
nad4 / 1338 / 6720 / 8057 / - / 0 / 67.41
nad4L / 300 / 8051 / 8350 / - / -7 / 66.67
trnT-ugu / 66 / 8353 / 8418 / + / 2 / 74.24
trnP-ugg / 67 / 8420 / 8486 / - / 1 / 71.64
nad6 / 522 / 8490 / 9011 / + / 3 / 71.26
cob / 1137 / 9011 / 10147 / + / -1 / 65.52
trnS-uga / 71 / 10168 / 10238 / - / 20 / 67.61
nad1 / 939 / 10256 / 11194 / - / 17 / 68.69
trnL-uaa / 66 / 11211 / 11277 / - / 16 / 60.61
rrnL / 1308 / 11295 / 12602 / - / 18 / 75.00
trnV-uag / 72 / 12603 / 12674 / - / 0 / 72.22
rrnS / 633 / 12680 / 13310 / - / 5 / 73.22
aBeginning of cox1 and end of rrnS failed to be sequenced.Actual size of these regions may not be significantly longer than shown here.
btRNA names are followed by their anticodon sequence
Supplemental Table S6. Profile of mitochondrial features of E. superba sample 760M
Accession Number: JQ286351% A/T: / 67.5 / % Unknown Bases: / 1.31 / Size: / 13312 bp
Position / Intergenic Nucleotides
Feature / Size / From / To / Strand / % A/T
Cox1a / 1473 / 1 / 1473 / + / 61.44
trnL-uagb / 66 / 1490 / 1555 / + / 16 / 62.12
cox2 / 688 / 1556 / 2243 / + / 0 / 64.24
trnK-uuu / 69 / 2244 / 2312 / + / 0 / 63.77
trnD-guc / 68 / 2313 / 2380 / + / 0 / 76.47
atp8 / 159 / 2381 / 2539 / + / 0 / 75.47
atp6 / 675 / 2533 / 3207 / + / -7 / 65.63
cox3 / 793 / 3207 / 3999 / + / -1 / 62.67
trnG-ucc / 67 / 4000 / 4066 / + / 0 / 67.16
nad3 / 354 / 4067 / 4420 / + / 0 / 64.12
trnA-ugc / 66 / 4420 / 4485 / + / 0 / 66.67
trnR-ucg / 67 / 4487 / 4553 / + / 1 / 61.19
trnN-guu / 65 / 4554 / 4618 / + / 0 / 73.85
trnS-ucu / 68 / 4619 / 4686 / + / 0 / 63.24
trnE-uuc / 69 / 4688 / 4756 / + / 1 / 72.46
trnF-gaa / 68 / 4856 / 4923 / - / 99 / 72.06
nad5 / 1731 / 4923 / 6653 / - / -1 / 67.88
trnH-gug / 66 / 6654 / 6719 / - / 0 / 69.70
nad4 / 1338 / 6720 / 8057 / - / 0 / 68.16
nad4L / 300 / 8051 / 8350 / - / -7 / 67.00
trnT-ugu / 66 / 8353 / 8418 / + / 2 / 74.24
trnP-ugg / 67 / 8420 / 8486 / - / 1 / 71.64
nad6 / 522 / 8490 / 9011 / + / 3 / 71.46
cob / 1137 / 9011 / 10147 / + / -1 / 65.52
trnS-uga / 71 / 10169 / 10238 / - / 20 / 67.61
nad1 / 939 / 10256 / 11194 / - / 17 / 69.44
trnL-uaa / 66 / 11211 / 11276 / - / 16 / 60.61
rrnL / 1308 / 11295 / 12602 / - / 18 / 75.08
trnV-uag / 72 / 12603 / 12674 / - / 0 / 72.22
rrnS / 633 / 12680 / 13312 / - / 5 / 73.30
aBeginning of cox1 and end of rrnS failed to be sequenced.Actual size of these regions may not be significantly longer than shown here.
btRNA names are followed by their anticodon sequence