Head: Assoc. Professor Stoyanka Stoitsova, PhD
Tel: +359 2 979 31 59
e-mail:
1979 / MS, Faculty of Biology, Sofia University ‘St. Kliment Ohridski’1980 / PhD Student, Central Laboratory of Helminthology, BAS
1983 / PhD, Central Laboratory of Helminthology, BAS
1984 / Research Sientist, Institute of Parasitology, BAS
1993 / Visiting Researcher, Queen’s University of Belfast, UK
1995 / Research Sientist, Institute of Microbiology, BAS
1999, 2001 / Visiting Researcher, University of Louisville, KY, USA
2006 / Associate Professor, Institute of Microbiology, BAS
2008 / Head of Section ‘Morphology of Microorganisms and Electron Microscopy’
Research Interests / Imaging techniques (confocal microscopy, TEM, SEM); Microbial ultrastructure; Bacteria-host cell interactions; Bacterial biofolms and biofilm modulation
PhD Students / Aneta Yoneva, Katia Georgieva, Anna Vacheva, Tsvetelina Paunova-Krasteva
Teaching / Methods of Electron Microscopy, Histochemistry, and Immunocytochemistry in Cell Biology (included in MS Programme “Cell Biology and Pathology”, Sofia University)
MS Students / Krasimira Vasileva, Bjulent Mustafa, Velichko Kamburov, Boriana Atanasova, Daiana Borisova, Viktoria Jordanova
STAFF
Assist. Professor Tsvetelina Paunova-Krasteva, MS,
Microbiologist Daiana Borisova, MS,
Laboratory Assistant Nina Tadjer, BS
Laboratory Assistant Lidana Panova
Electronic Engineer Ivan Chavdarov, MS
Viktoria Jordanova, MS Student
Resarch Area
Sporulation, benign tumor from patient, virus-infected cells, yeasts
Ultrastructure and cytochemistry of microorganisms from diverse phylogenetic groups – viruses, prokaryotes (bacteria, actinomycetes) and eukaryotes (yeast, fungi).
Bacterial adherence to abiotic surfaces and cultured cells.
Surface polysaccharide antigens of pathogenic E. coli strains
Bacterial phenotypes and phenotypic variations
Biofilms – structure, development and modulation by natural products
E. coli O157:H7 (food borne pathogen causing haemolytic-uremic syndrome) MBL binding
Projects
COST ActionBM1003 “Microbial cell surface determinants of virulence as targets for new therapeutics in Cystic fibrosis”. European network including 16 EU countries. Project co-ordinator: Professor A. Molinaro, Federico II University, Naples, Italy; Secretary: Dr R. Berisio, Federico II University, Naples, Italy; Co-ordinator for Bulgaria: Dr S. Stoitsova.
“Morphogenesis and development of intestinal glycocalyx – a guard against infection”. NSF, Contract B01/30, 2012. Principal investigator E. Nikolova, IEMPAM, BAS. Participants from the Institute of Microbiology: S. Stoitsova, T. Paunova-Krasteva.
Escherichia coli O157: novel approaches to an emerging pathogen. NSF, Contract DO02-301/2008. Principal investigator S. Stoitsova; Participants from the Institute of Microbiology: R. Ivanova, T. Paunova-Krasteva, A. Vacheva, N. Tadjer and L. Dobreva; Other consortium members: Federico II University of Naples, Italy, IEMPAM, BAS, Institute of Organic Chemistry, BAS, and Department of Cytology, Histhology and Embryology, Faculty of Biology, Sofia University.
Biofilms of Escherichia coli – a risk factor for contamination of medical devices: Biofilm structure, effects of biogenic factors and search of antiadhesives and biofilm inhibitors. NSF, Contract VU-L-321/2007. Principal investigator S. Kostadinova, Plovdiv University “P. Hilendarski”; Participants from the Institute of Microbiology: S. Stoitsova, A. Vacheva (PhD student), S. Danova, R. Ivanova,Ts. Paunova-Krasteva, N. Tadjer
Confocal laser scanning microscope for evaluation of Bulgarian bioresources. NSF, Contract IFS-B-603/2007. Coordinator S. Stoitsova, and 8 participants from the Institute of Microbiology; Other consortium members: IBER, BAS, and IEMPAM, BAS
Sub-micron machining and analysis of materials and structures using scanning electron microscopy in combination with focused ion beam. NSF, Contract DО02-56/10.12.2008. Coordinator S. Rusev. Participants from the Institute of Microbiology: S. Stoitsova, T. Paunova, A. Vacheva. Other consortium members: Sofia University, ISSP, BAS, University of Mining and Geology, University of Transport
Selected Publications
Tropcheva R., Lesev N., Danova S., Stoitsova S., Kaloyanova S. , 2015. Novel cyanine and homodimeric styryl dyas as fluorescent probes for assessment of lactic acid bacteria cell viability. J. Photochem. Photobiol. B: Biology, http://www.sciencedirect.com/science/article/pii/S1011134415000068
Doumanov J.; Mladenova K.; Topouzova-Hristova T.; Stoitsova S.; Petrova S., 2015. Vipoxin and its components affect proliferation and cell death in HepG2 cells, Toxicon 94, 36-44. doi: 10.1016/j.toxicon.2014.12.009. PMID: 25534906
Stoitsova S., Paunova-Krasteva Ts., Pavlova V., Nikolova E., 2014. Stimulated gut differentiation and the risks of bacterial infection. Compt. rend. Acad. bulg. Sci. 67(2), 211-216.
Paunova-Krasteva Ts., Pavlova V., DeCastro C., Ivanova R., Molinaro A., Nikolova E., Stoitsova S., 2014. Cyclic enterobacterial common antigens from Escherichia coli O157 as microbe-associated molecular patterns. Canadian Journal of Microbiology 60, 173-176. Doi 10.1139/cjm-2013-0697. PMID: 24588391
Abrashev R., Stoitsova S., Krumova E., Pashova S., Paunova-Krasteva T., Vassilev S., Dolashka-Angelova P., Angelova M., 2014. Temperature stress tolerance of the fungal strain Aspergillus niger 26: physiological and ultrastructural changes. World J Microbiol Biotechnol 30, 1661-1668. doi: 10.1007/s11274-013-1586-8, PMID: 24366816
De Soyza, A., Hall, A., Mahenthiralingam, E., Drevinek, P., Kaca, W., Drulis-Kawa, Z., Stoitsova S., Toth, V., Coenye, T., Zlosnik, J., Burns, J., Correia, I., De Vos, D., Pirnay, J.-P., Kidd, T., Reid, D., Manos, J., Klockgether, J., Wiehlmann, L., Tummler, B., McClean, S., Winstanley, C., 2013. Developing an international Pseudomonas aeruginosa reference panel. MicrobiologyOpen, doi: 10.1002/mbo3.141, PMID:24231377.
Nikolova K., Kaloyanova S., Mihaylova N., Stoitsova S., Chausheva S., Vsilev A., Lesev N., Dimitrova P., Deligeorgiev T., Tchorbanov A., 2013. New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy. J. Photochem. Photobiol. B, 129C, 125-134, doi: 10.1016/j.jphotobiol.2013.10.010, PMID: 24214409.
Topouzova-Hristova T., Atanasova B., Strateva T., Paunova-Krasetva Ts., Stephanova E., Mitov I., Stoitsova S., 2013. Comparative study of the cytotoxic effects of two P. aeruginosa cystic fibrosis isolates. Science and Technologies Vol. III, No. 1, 1-4.
Vacheva A, Ivanova R, Paunova-Krasteva T, Stoitsova S., 2012. Released products of pathogenic bacteria stimulate biofilm formation by Escherichia coli K-12 strains. Antonie Van Leeuwenhoek 102(1), 105-19, doi: 10.1007/s10482-012-9718-y PMID: 22419503.
Fregolino E, Ivanova R, Lanzetta R, Molinaro A, Parrilli M, Paunova-Krasteva T, Stoitsova SR, De Castro C., 2012. Occurrence and structure of cyclic Enterobacterial Common Antigen in Escherichia coli O157:H(-). Carbohydr Res. 363, 29-32, doi: 10.1016/j.carres.2012.09.017. PMID: 23103511.
Sotirova A, Avramova T, Stoitsova S, Lazarkevich I, Lubenets V, Karpenko E, Galabova D., 2012, The importance of rhamnolipid-biosurfactant-induced changes in bacterial membrane lipids of Bacillus subtilis for the antimicrobial activity of thiosulfonates. Curr Microbiol., 65(5):534-41, PMID: 22810959.
Vacheva A., Georgieva R., Danova S., Ivanova R., Marhova M., Kostadinova S., Vasileva K., Bivolarska M., Stoitsova S., 2012. Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli. Central Eur J Biology 7, 219-229, doi: 10.2478/s11535-012-0004-9.
Krumova E., Stoitsova S.R., Paunova-Krasteva T, Pashova, Angelova M., 2012. Copper stress and filamentous fungus Humicola lutea 103 — ultrastructural changes and activities of key metabolic enzymes. Canadian Journal of Microbiology 58(12), 1335-43. doi: 10.1139/w2012-112. PMID: 23210990.
Stoitsova S., Topouzova-Hristova T., Stephanova E., 2012. Bacterial interference with host epithelial junctional complexes: Probiotic bacteria vs. A/E lesion-forming E. coli. Journal of BioScience and Biotechnology, 1(2), 83-89, ISSN: 1314-6246.
Stoitsova S., Vacheva A., Paunova-Krasteva T., Ivanova R., Danova S., Manasiev J. 2012. The multicellular behavior of Escherichia coli: a target for interferences. In: H. Najdenski (Ed), New Trends in Microbiology, The Stephan Angeloff Institute of Microbiology, Sofia, ISBN 978-954-92882-1-6, pp. 147-158.
Stoitsova S., Topouzova-Hristova T., Stephanova, E., 2012. Alteration of proteins engaged in vesicular trafic in HeLa cells co-cultivated with Escherichia coli O157:H-. Compt. rend. Acad. bulg. Sci. 65(11) 1569-1574.
Velinov T, Asenovska Y, Marinkova D, Yotova L, Stoitsova S, Bivolarska M, Stavitskaya L. 2011. Total internal reflection imaging of microorganism adhesion using an oil immersion objective. Colloids Surf B Biointerfaces. 88(1):407-12, doi: 10.1016/j.colsurfb.2011.07.022. PMID: 21820880.
Vacheva A., Mustafa B., Staneva J., Marhova M., Kostadinova S., Todorova M., Ivanova R., Stoitsova S., 2011. Effects of extracts from medicinal plants on biofilm formation by Escherichia coli urinary tract isolates. Biotechnol Biotechnol Equipment, 25(4), 92-97, doi: 10.5504/BBEQ.2011.0111.
Paunova Ts., Ivanova R., Stoitsova S., 2010. Growth temperature-related cell surface changes of Escherichia coli O157:H-. Biotechnol. Biotechnol. Eq. ISSN 1310-2818, 24SE, 585-588.
Marhova M., Kostadinova S, Stoitsova S., 2010. Biofilm-forming capabilities of urinary Escherichia coli isolates. Biotechnol. Biotechnol. Eq., ISSN 1310-2818, 24SE, 589-593.
Stoitsova S., Ivanova R., Paunova T., 2007. Biofilm formation by reference strains of Escherichia coli. Compt. rend. Acad. bulg. Sci. 60, 71-75.
Bivolarska M., Velinov T., Stoitsova S. 2006. Guided-wave and ellipsometric imaging of supported cells. Journal of Microscopy, 224, 242-248, PMID: 17210056.
Boteva R., Bogoeva V., Stoitsova S., 2005. PA-I lectin from Pseudomonas aeruginosa binds acyl homoserine lactones. Biochim. Biophys. Acta 1747, 143-149, PMID: 15698948.
Stoitsova S., R. Ivanova, I. Dimova, 2004. Lectin-binding epitopes at the surface of Escherichia coli K-12: examination by electron microscopy, with special reference to the presence of a colanic acid-like polymer. J. Basic Microbiol. 44(4) 296-304, PMID: 15266602.
Stoitsova S., Boteva R., Doyle R.J., 2003. Binding of hydrophobic ligands by Pseudomonas aeruginosa PA-I lectin. Biochim. Biophys. Acta 1619, 213-219, PMID: 15266602.
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