62 genes differentially expressed between aniogenic and non-angiogenic tumours.
Angio/non-angio
CELL GROWTH AND DIFFERENTIATION
FOS1.8nucleus
CAMK2D1.55 cytoplasm
SEMAE1.52membrane
NRGN1.37nucleus
PFN20.69ERcytoplasm
GMPS0.67mitochondriacytoplasm
PLXNA2 0.74membrane
VESICLES/PROTEIN TRANSPORTERS
CLTA2.08membrane
LCAT1.37membraneERmitochondriacytoplasm
NAPG1.34 ER/golgicytoplasm
STXBP11.33mitochondrianucleus
ANXA70.78cytoplasmnucleus
SURF-40.75membrane
SIGNALING CASCADE
SIPA1L11.5cytoplasm
PTP4A20,73ERcytoplasm
GNAI10.72membrane
TRANSCRIPTION/PROTEIN SYNTHESIS
CGI-071.48cytoplasmnucleus
STK22A1.45nucleus
PHF151.29nucleus
HMG2L10.82nucleus
POLR2K 0.78nucleus
HNRNPG-T0.77nucleus
HMGN40.74mitochondrianucleus
ZNF2170.72cytoplasmnucleus
DARS0.71cytoplasm
GTF2A20.69nucleus
MCM60.69cytoplasmnucleus
HNRPA2B10.67cytoplasmnucleus
GSCL0.66nucleus
DHX150.66ERcytoplasmnucleus
HMGN10.52mitochondriacytoplasmnucleus
METABOLISM/MITOHONDRIAL RESPIRATORY CHAIN
GCDH0.84mitochondriacytoplasm
GPI0.81mitochondriacytoplasm
NDUFB60.73mitochondria cytoplasmnucleus
MDH10.72cytoplasmextracellular
ATP5O0.72mitochondria
ANXA70.78cytoplasmnucleus
ATP6V1G10.71membranenucleus
PRSS150.66mitochondriacytoplasmnucleus
CELL CYCLE
SMYD20.72cytoplasmnucleus
SET0.69cytoplasmnucleus
MCM60.69cytoplasmnucleus
CKS20.67nucleus
H2AV0.66nucleus
RPA30.64cytoplasmnucleus
H2AFZ0.58ER cytoplasmnucleus
CDK2AP10.57nucleus
APOPTOSIS
FOS1.8nucleus
PRODH1.8mitochondriacytoplasm
FAH1.59cytoplasm
ANXA70.78cytoplasmnucleus
SOD10.68cytoplasmnucleus
ANGIOGENESIS AND REMODEELING/CELL ADHESION
ITGB41.54membranemitochondriacytoplasm
PIK3R11.48cytoplasmnucleus
ARHE1.48cytoplasm
COL7A11.47mitochondria
TSP11.41
INFLAMMATION
FOS1.8nucleus
SEMAE1.52membrane
TSP11.41cytoplasmextracellular
PLXNA20.74membrane
CELL MOTILITY
VAPA0.77ER
PROTEASOME
GA170.75cytoplasm
PSMB10.72ERcytoplasm
UBIQUITINE
STUB11.56cytoplasm
KIAA03221.42cytoplasmextracellular
DRUG RESISTANCE
SEMAE1.52membrane
PLXNA2 0.74membrane
FUNCTION UNKNOWN
ID 42388_B1.69UNKNOWN
KIAA06480.77cytoplasm
ITM2A0.72membranemitochondria
GENERAL LIST
62 genes differentially expressed between aniogenic and non-angiogenic tumours (up in angio)
Angio/
Non-angio
CLTA2.08membrane
FOS1.8nucleus
PRODH1.8mitochondriacytoplasm
ID 42388_B1.69UNKNOWN
FAH1.59cytoplasm
STUB11.56cytoplasm
CAMK2D1.55 cytoplasm
ITGB41.54membranemitochondriacytoplasm
SEMAE1.52membrane
SIPA1L1 1.5cytoplasm
PIK3R11.48cytoplasmnucleus
ARHE1.48cytoplasm
CGI-071.48cytoplasmnucleus
COL7A1 1.47mitochondria
STK22A1.45nucleus
KIAA03221.42cytoplasmextracellular
TSP11.41cytoplasmextracellular
NRGN1.37nucleus
LCAT1.37membraneERmitochondriacytoplasm
NAPG1.34 ER/golgicytoplasm
STXBP11.33mitochondrianucleus
PHF151.29nucleus
62 genes differentially expressed between aniogenic and non-angiogenic tumours (up in non-angio)
Angio/non-angio
GCDH0.84mitochondriacytoplasm
HMG2L0.82nucleus
GPI0.81mitochondriacytoplasm
ANXA70.78cytoplasmnucleus
POLR2K 0.78nucleus
VAPA0.77ER
KIAA06480.77cytoplasm
HNRNPG-T0.77nucleus
SURF-40.75 membrane
GA170.75cytoplasm
PLXNA2 0.74membrane
HMGN40.74mitochondrianucleus
PTP4A20,73ERcytoplasm
NDUFB60.73mitochondria cytoplasmnucleus
ZNF2170.72cytoplasmnucleus
MDH10.72cytoplasmextracellular
PSMB10.72ERcytoplasm
GNAI10.72membrane
SMYD20.72cytoplasmnucleus
ITM2A0.72membranemitochondria
ATP5O0.72mitochondria
ATP6V1G10.71membranenucleus
DARS0.71cytoplasm
TAF-IBETA 0.7 nucleus
SET0.69cytoplasmnucleus
PFN20.69ERcytoplasm
GTF2A20.69nucleus
MCM60.69cytoplasmnucleus
SOD10.68cytoplasmnucleus
CKS20.67nucleus
HNRPA2B10.67cytoplasmnucleus
GMPS0.67mitochondriacytoplasm
GSCL0.66nucleus
PRSS150.66mitochondriacytoplasmnucleus
H2AV0.66nucleus
DHX150.66ERcytoplasmnucleus
RPA30.64cytoplasmnucleus
H2AFZ0.58ER cytoplasmnucleus
CDK2AP10.57nucleus
HMGN10.52mitochondriacytoplasmnucleus
PATHWAYS
CELL GROWTH AND DIFFERENTIATION
FOS1.8v-fos FBJ murine osteosarcoma viral oncogene homologue.
CAMK2D1.55Calcium/calmodulin-dependent protein kinase (CaM kinase) II delta. The identification of these new, potential tumour-specific CaMK-II variants supports previous indications that CaMK-II plays a role in growth control.
SEMAE1.52Semaphorin E.
NRGN1.37Neurogranin. Protein kinase substrate that binds calmodulin in absence of calcium
PFN20.69Profilin II. A 14-kDa protein responsible for keeping the cytoskeletal protein actin monomeric under ionic conditions that promote its polymerization into filaments.
GMPS0.67Guanine monophosphate synthetase. Amination of XMP to GMP. It is essential for de novo purine synthesis
PLXNA20.74Plexin A2. Putative Semaphorin receptor
VESCICLES/PROTEIN TRANSPORTERS
CLTA2.08Clathrin, light peptide (Lca). Main structural component of the cytoplasmic face of the pits and vesicles in which macromolecules are entrapped in the process of receptor-mediated endocytosis
LCAT1.37Lecithin-cholesterol acyltransferase. Extracellular cholesterol esterifying enzyme. The esterification of cholesterol is required for cholesterol transport. Phosphatidylcholine-sterol O-acyltransferase Definition: Catalysis of the reaction: phosphatidylcholine + a sterol = a sterol ester + 1-acylglycerophosphocholine.
NAPG1.34N-ethylmaleimide-sensitive factor attachment protein, gamma. Membrane fusion.
STXBP11.33Syntaxin binding protein 1.
ANXA70.71Annexin A7. Calcium-dependent phospholipid binding proteins family. Membrane fusion in exocytosis. Mediates galectin-3 translocation to the perinuclear mitochondrial membranes, where it regulates mitochondrial integrity critical for apoptosis regulation
SURF-40.75Surfeit 4. The yeast homologous is involved in non-clathrin-coated vesicular coat proteins (COPs)
SIGNALING CASCADE
SIPA1L11.5Signal-induced proliferation-associated 1-like 1.
PTP4A20.73Protein tyrosine phosphatase type IVA, member 2. Putative oncogene.
GNAI10.72 Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1.
TRANSCRIPTION / PROTEIN SYNTHESIS
FOS1.8v-fos FBJ murine osteosarcoma viral oncogene homologue. Specific RNA polymerase II transcription factor
CGI-071.48CGI-07 protein. Nonsense mediated mRNA decay. Export of ribosome from nucleus to cytoplasm.
STK22A1.45Serine/threonine kinase 22A (spermiogenesis associated). Regulation of transcription. DNA binding
PHF151.29PHD finger protein 15. DNA Binding
HMG2L0.82High-mobility group (nonhistone chromosomal) protein 2-like 1. Regulation of transcription, DNA-dependent
POLR2K0.78Polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa. This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases.
HNRNPG-T0.77Testes-specific heterogeneous nuclear ribonucleoprotein G-T. DNA-Binding. Regulation of transcription
HMGN40.74High-mobility group nucleosomal binding domain 4. It is thought to reduce the compactness of the chromatin fibre in nucleosomes, thereby enhancing transcription from chromatin templates.
ZNF2170.72Zinc finger. Protein 217. Regulation of transcription, DNA-dependent. Strong candidate oncogene
DARS0.71Aspartyl-tRNA synthetase. It is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. It has ATP binding activities
GTF2A20.69General transcription factor IIA, 2, 12kDa. RNA polymerase II transcription factor activity. From Pfam homology, the products would be involved in transcription initiation from Pol II promoter and would localize in transcription factor TFIIA.
MCM60.69Minichromosome maintenance deficient 6 (MIS5 homology, S. pombe). The protein encoded by this gene is one of the highly conserved MCM that are essential for the initiation of eukaryotic genome replication.
HNRPA2B10.67Heterogeneous nuclear ribonucleoprotein A2/B1
GSCL0.66Goosecoid like. GSCL a homeodomain-containing gene. Regulation of transcription from PolII promoter. Regulation of transcription, DNA-dependent.
DHX150.66DEAD/DEAH (Asp-Glu-Ala-His) box polypeptide 15. Putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.
HMGN10.52High-mobility group nucleosome binding domain 1. Member of the HMG 14/17 family of proteins; affects rate of elongation by RNA polymerase II on chromatin templates
METABOLISM/ MITOCHONDRIAL RESPIRATORY CHAIN
GCDH0.84Glutaryl-coenzyme A dehydrogenase. It is a multifunctional enzyme responsible for the dehydrogenation and decarboxylation of glutaryl-CoA to crotonyl-CoA in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism.
GPI0.81Glucose phosphate isomerase. Energy pathway
NDUFB60.73NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa. Complex I (NADH: ubiquinone oxidoreductase), the first multisubunit enzyme complex of the mitochondrial respiratory chain, Expressed in normal tissues with high energy demand.
MDH10.72Malate dehydrogenase 1, NAD (soluble). Catalyses the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
ATP5O0.72ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (core Delta).
ANXA70.71Annexin A7. Calcium-dependent phospholipid binding proteins family. Membrane fusion in exocytosis. Mediates galectin-3 translocation to the perinuclear mitochondrial membranes, where it regulates mitochondrial integrity critical for apoptosis regulation
ATP6V1G10.71Vacuolar ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 1. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles (e.g. lysosomes).
PRSS15 0.66 Protease, serine, 15. In assays of protein purified from bovine heart mitochondria, Bota and Davies (2002) found that the PRSS15 selectively recognized and degraded the oxidized, hydrophobic form of aconitase (ACO2; 100850) after mild oxidative modification. Severe oxidation resulted in aconitase aggregation, making it a poor PRSS15 substrate. Down regulation of PRSS15 expression in human lung fibroblasts caused accumulation of oxidatively modified aconitase. Bota and Davies (2002) concluded that PRSS15 might prevent extensive oxidation, aggregation, and accumulation of aconitase, which could otherwise compromise mitochondrial function and cellular viability.
CELL CYCLE
SMYD2072SET and MYND domain containing 2. SET: methylation of histones. MYND: This domain is found in some suppressors of cell cycle entry.
SET0.69 SET translocation (myeloid leukaemia-associated).DNA replication factor (template activating factor I, beta isoform); potent and specific inhibitor of protein phosphatase 2A (PPP2CA); has an acidic C-terminus
MCM60.69Minichromosome maintenance deficient 6. Play roles in the progression of the cell cycle bind to the DNA after mitosis and enable DNA replication before being removed during S phase.
CKS20.67CDC28 protein kinase regulatory subunit 2 and CDC2-ASSOCIATED PROTEIN. It is required for the first metaphase/anaphase transition of mammalian meiosis.
H2AV0.66Histone H2A. F/Z variant.
RPA30.64 Replication protein A3, 14kDa. The products would have single-stranded DNA binding activity, would be involved in DNA replication, DNA repair.
H2AFZ0.58 H2A histone family, member Z.
CDK2AP10.57CDK2-associated protein 1. (Doc-1 for 'deleted in oral cancer.). It is thought to negatively regulate CDK2 activity. This protein was found to also interact with DNA polymerase alpha, which suggested the regulatory role in DNA replication during S phase of the cell cycle.
APOPTOSIS
FOS1.8v-fos FBJ murine osteosarcoma viral oncogene homologue.
PRODH1.8Proline dehydrogenase (oxidase) 1. First step in proline catabolism. . Proline oxidase induces apoptosis in tumour cells
FAH1.59 Fumarylacetoacetate hydrolase (fumarylacetoacetase). Tyrosine and phenylalanine catabolism pathway. ? Induction of apoptosis
ANXA70.71Annexin A7. Calcium-dependent phospholipid binding proteins family. Mediates galectin-3 translocation to the perinuclear mitochondrial membranes, where it regulates mitochondrial integrity critical for apoptosis regulation.
SOD10.68Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)). Protection from apoptosis.
ANGIOGENESIS AND REMODELLING/CELL ADHESION
ITGB41.54Integrin, B4. As a component of aVb4 induces VEGF translation. Alpha-6/beta-4 increased carcinoma invasion in a PI3K-dependent manner. Epidermolysis bullosa
(GPRK2L 1.42G protein-coupled receptor kinase 2-like (Drosophila). Part of PI3K pathway)
PIK3R11.48Phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha). ITGB pathway. PDECGF
ARHE1.48Ras homologue gene family, member E. ITGB pathway. Negative regulators of actin assembly and of cell adhesion.
COL7A11.47Collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive). Squamous epithelium cell adhesion. Epidermolysis bullosa
TSP11.41Thrombospondin 1.
INFLAMMATION/IMMUNE RESPONSE
FOS1.8v-fos FBJ murine osteosarcoma viral oncogene homologue.
SEMAE1.52Semaphorin E. Expressed in rheumatoid Arthritis. Immunosuppressive
TSP11.41Thrombospondin 1.
PLXNA2 0.74Plexin A2. Putative Semaphorin receptor
CELL MOTILITY
(VAMP0.78Vesicle-associated membrane protein. Contains major sperm protein (MSP) domain)
VAPA0.77VAMP (Vesicle-associated membrane protein) associated protein. A, 33kDa.
PROTEASOME
Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
GA170.75dendritic cell protein. Motif in proteasome subunits, Int-6, Nip-1 and TRIP-15; also called the PCI (Proteasome, COP9, Initiation factor 3) domain. Unknown function
PSMB10.72 Proteasome (prosome, macropain) subunit, beta type, 1. From LocusLink Proteome or GOA annotation, the products would have peptidase activity. From Pfam homology, the products would have proteasome endopeptidase activity, would be involved in ubiquitin-dependent protein catabolism and would localize in 20S core proteasome.
UBIQUITIN PATHWAY
Ubiquitin ligases determine protein stability in a highly regulated manner by coordinating the addition of polyubiquitin chains to proteins that are then targeted to the proteasome for degradation.
STUB11.56STIP1 homology and U-box (Ubiquitin-ligases) containing protein 1. CHIP decreases net ATPase activity and reduces chaperone efficiency, and they implicate CHIP in the negative regulation of the forward reaction of the Hsc70-Hsp70 substrate-binding cycle.
KIAA03221.42HECT type E3 ubiquitin ligase.
DRUG RESISTANCE
SEMAE1.52Semaphorin E.
PLXNA2 0.74Plexin A2. Putative Semaphorin receptor
FUNCTION UNKNOWN
ID 42388_B1.69yh08e04.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE: 42388 3'. Function unknown
KIAA06480.77KIAA0648 protein (Blast score 2189). Function unknown
ITM2A0.72 Integral membrane protein 2A. Function unknown.
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