Table S1 Summary of the gene name and products of cobalt-sensitive mutants
Function category/CoCl2 sensitivity / Systematic Name / Common Name / Product
Histidine biosynthesis
+++ / SPBC11B10.02c / his3 / histidinol-phosphate aminotransferaseimidazoleacetol phosphate transaminase His3
+++ / SPBC21H7.07c / his5 / imidazoleglycerol phosphate dehydratase
+++ / SPAC3F10.09 / his6* / 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino) methylideneamino]imidazole-4-carboxamide isomerase
+++ / SPBC29A3.02c / his7 / phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphohydrolase His7
++ / SPBC1711.13 / his2 / histidinoldehydrogenase His2
++ / SPAC25G10.05c / his1 / ATP phosphoribosyltransferase
Mitochondria function
+++ / SPBC3H7.03c / kgd1
/ogd1* / 2-oxoglutarate dehydrogenase (lipoamide)
e1 component of oxoglutaratedehydrogenase complex
+++ / SPAC1F5.07c / hem14 / protoporphyrinogenoxidase
++ / SPBP4H10.03 / oxa102 / mitochondrial inner membrane translocase Oxa102
++ / SPAC14C4.14 / atp1 / F1-ATPase alpha subunit
+ / SPAC23C4.11 / atp18 / F-0 ATPase subunit J
+ / SPAC750.08c / mae1* / NAD-dependent malic enzyme
+ / SPAC3A12.12 / atp11 / F1-ATPase chaperone Atp11
+ / SPAC823.10c / N/A / mitochondrial carrier with solute carrier repeats
Ubiquitination
+++ / SPAC6B12.07c / N/A / ubiquitin-protein ligase E3
+++ / SPAC1486.02c / ucp14 / UBA domain protein Ucp14
+++ / SPBC947.10 / dsc1 / ubiquitin-protein ligase E3
+ / SPBC887.04c / lub1 / WD repeat protein Lub1
+ / SPBC21D10.09c / rkr1* / ubiquitin-protein ligase E3
+ / SPAC26A3.16 / dph1 / UBA domain protein Dph1
Gene expression and chromatin remodeling
+ / SPAC1783.07c / pap1 / transcription factor Caf3
+ / SPAC23C11.08 / php3 / CCAAT-binding factor complex subunit Php3
+ / SPBC1718.07c / zfs1 / CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism
+ / SPAC26H5.05 / spt23/
mga2* / IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis
+ / SPCC24B10.08c / ada2 / SAGA complex subunit Ada2
+ / SPAC19G12.02c / pms1 / MutL family mismatch-repair protein Pms1
Transporter
+++ / SPAC23C11.14 / zhf1 / zinc ion transporter Zhf1
++ / SPCC965.13 / N/A / pyradoxal family membrane transporter
Membrane trafficking
+++ / SPAC823.05c / tlg2 / SNARE Tlg2
+++ / SPAC144.06 / apl5 / AP-3 adaptor complex subunit Apl5
+ / SPBC119.12 / rud3/
grp1* / Golgi matrix protein
AAA family ATPase
++ / SPAC644.14c / rhp51 / recombinase Rhp51
+ / SPAC31G5.19 / yta7* / ATPase with bromodomain protein
+ / SPAC328.04 / sap1/
yta6* / AAA family ATPase, unknown biological role
Variety of other known functions
+++ / SPAPB1E7.02c / mcl1 / DNA polymerase alpha accessory factor Mcl1
+++ / SPBC18H10.02 / lcf1 / long-chain-fatty-acid-CoAligase Lcf1
+++ / SPBC4F6.06 / kin1 / microtubule affinity-regulating kinase Kin1
++ / SPBC365.14c / uge1 / UDP-glucose 4-epimerase
++ / SPAC13G6.09 / tsr4* / SSU-rRNA maturation protein Tsr4 homolog 2
+ / SPBP35G2.14 / N/A / RNA-binding protein
+ / SPAC25B8.01 / dap1 / cytochrome P450 regulator Dap1
+ / SPAPB17E12.08 / eos1* / N-glycosylation protein
+ / SPAP8A3.04c / hsp9 / heat shock protein Hsp9
+ / SPAC6F6.01 / cch1 / calcium channel Cch1
+ / SPBC4C3.08 / mug136 / acetylglucosaminyltransferase
+ / SPAC10F6.13c / aat2* / aspartateaminotransferase
+ / SPAC23C11.02c / rps23 / 40S ribosomal protein S23
Unknown functions
+++ / SPBC577.11 / N/A / sequence orphan
+++ / SPAC20H4.02 / dsc3 / conserved fungal protein
+++ / SPAC4D7.11 / dsc4 / conserved fungal protein
++ / SPAC31A2.14 / wdr48 / WD repeat protein, human WRDR48 family
+ / SPAC56F8.12 / N/A / conserved fungal protein
+ / SPBC2F12.12c / N/A / conserved eukaryotic protein
+ / SPBC29A3.13 / pdp1 / PWWP domain protein
+++ indicates that the cells completely failed to growth on YPDA plates containing 3.5 mMCoCl2.
++ indicates that tiny colonies were observed to grow on YPDA plates containing 3.5 mMCoCl2.
+ indicates that colonies were observed on YPDA plates containing 3.5 mMCoCl2, however, the size of the colonies were significantly smaller than that of the wild-type cells.
* indicates that the naming of genes is after the S. cerevisiaecounterparts as the common name in S. pombe is not available.
N/A indicates that common gene name is not available.
Table S2 Summary of the gene name and products of cobalt-resistant mutants
Function category/CoCl2 resistance / Systematic Name / Common Name / Product
Mitochondria function
+++ / SPBC27.06c / mgr2 / mitochondrial membrane protein Mgr1
+++ / SPBC4F6.08c / mrpl39 / mitochondrial ribosomal protein subunit L39
+++ / SPAC9G1.04 / oxa101 / mitochondrial inner membrane translocase Oxa101
+++ / SPCC1682.01 / qcr9 / ubiquinol-cytochrome-c reductase complex subunit 10
++ / SPAC22F3.07c / atp20 / F0-ATPase subunit G
++ / SPBC25B2.04c / mtg1 / mitochondrial GTPase involved in translation Mtg1
++ / SPBC660.10 / mef2* / mitochondrial translation elongation factor G
+ / SPAC13G7.11 / mba1* / mitochondrial respiratory complex assembly protein (predicted)
+ / SPCC4B3.03c / mam3* / mitochondrial morphology protein (predicted)
+ / SPAC328.09 / odc2* / mitochondrial 2-oxoadipate and 2-oxoglutarate transporter
+ / SPBC31F10.15c / atp15 / F0-ATPase epsilon subunit
signal transduction
+++ / SPBC8D2.01 / gsk31 / serine/threonine protein kinase Gsk31
+++ / SPAC3C7.06c / pit1 / serine/threonine protein kinase Pit1
+++ / SPBC776.04 / sec2302 / GTPase activating protein Sec23b
+++ / SPAC4A8.03c / ptc4 / protein phosphatase 2C Ptc4
+++ / SPBC887.10 / mcs4 / two-component response regulator
++ / SPAC1006.09 / win1 / MAP kinasekinasekinase Win1
++ / SPBC409.07c / wis1 / MAP kinasekinase Wis1
++ / SPAC22E12.14c / sck2 / serine/threonine protein kinase Sck2
+ / SPAC3G9.05 / spa2* / GTPase activating protein
+ / SPCC830.06 / cnb1 / calcineurin regulatory subunit
Gene expression and chromatin remodeling
++ / SPBC15D4.10c / amo1 / nuclear rim protein Amo1
++ / SPAC3H8.09c / nab3 / poly(A) binding protein Nab3
++ / SPAC2F7.04 / pmc2 / RNA polymerase II holoenzyme mediator complex subunit
++ / SPBC28F2.02 / mep33 / mRNA export protein Mep33
++ / SPAC30D11.07 / nth1 / DNA endonuclease III
++ / SPCC285.16c / msh6 / MutS protein homolog
++ / SPAC17G8.13c / mst2 / histoneacetyltransferase Mst2
++ / SPCC1235.09 / hif2 / histonedeacetylase complex subunit
Ubiquitination
++ / SPAC343.18 / rfp2 / ubiquitin-protein ligase E3
++ / SPBC19C7.02 / ubr1 / N-end-recognizing protein Ubr1
++ / SPAC19A8.10 / rfp1 / ubiquitin-protein ligase E3
+ / SPAC6G10.11c / ubi3 / ribosomal ubiquitin fusion protein Ubi3
+ / SPBC31F10.10c / mub1 / zf-MYND type zinc finger protein
Transporter
++ / SPBC1683.10c / pcl1 / ferrous iron transporter Pcl1
+ / SPCC1884.02 / nic1 / NiCoT heavy metal ion transporter Nic1
+ / SPAC664.14 / amt2 / ammonium transporter Amt2
Variety of other known functions
+++ / SPBC36B7.03 / sec63 / ER protein translocation subcomplex subunit Sec63
++ / SPAC17C9.02c / lys7 / alpha-aminoadipatereductasephosphopantetheinyltransferase
++ / SPBC2G2.08 / ade9 / C-1-tetrahydrofolatesynthase
/methylenetetrahydrofolatedehydrogenase
/methylenetetrahydrofolatecyclohydrolase
/ formyltetrahydrofolatesynthetase(predicted)
++ / SPBC119.06 / sco1 / copper chaperone Sco1
++ / SPCC4F11.04c / imt2 / mannosyltransferase complex subunit
++ / SPBC56F2.03 / arp10 / actin-like protein Arp10
++ / SPBP4H10.19c / N/A / calreticulin/calnexin homolog
++ / SPAC3F10.04 / gsa1 / glutathione synthetase large subunit Gsa1
++ / SPAC1783.01 / N/A / methioninesynthasereductase (predicted)
++ / SPBC1604.02c / N/A / sequence orphan
++ / SPBC21D10.12 / hob1 / BAR adaptor protein Hob1
+ / SPCC594.04c / N/A / steroid oxidoreductasesuperfamily protein
+ / SPBC947.05c / fre1* / ferric-chelatereductase
+ / SPBC691.03c / apl3 / AP-2 adaptor complex subunit Alp3
+ / SPCC613.12c / raf1 / Rik1-associated factor Raf1
+ / SPCC1442.02 / ybp2* / central kinetochore associated family protein (predicted)
+ / SPCC1020.11c / emc6* / ER membrane protein complex subunit 6
+ / SPBC18H10.06c / swd2 / COMPASS complex subunit Swd2
+ / SPAC3H1.11 / hsr1 / transcription factor Hsr1
+++ indicates that the deletion cells grow well on the plates containing 7 mMCoCl2.
++ indicates that small colonies were observed on the plates containing 7 mMCoCl2, while big colonies were observed on the plates containing 6 mMCoCl2.
+ indicates that no colonies were observed on the plates containing 7 mMCoCl2, while small colonies were observed on the plates containing 6 mMCoCl2.
* indicates that the naming of genes is after the S. cerevisiaecounterparts as the common name in S. pombe is not available.
N/A indicates that common gene name is not available.