Selected Examples of Crosses Between Free-Living P. Trichosuri Females and Males

Kulkarni et al. Supplementary Table 1:

Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP184 (336) / ytP53 / X
Female 1 / CT
Female 2 / CT
Male / T
Female progeny / 6 x CT
Male progeny / 2 x C

Selected examples of crosses between free-living P. trichosuri females and males

Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP183 (227) / ytP67 / X
Female 1 / AG
Female 2 / A
Male / G
Female progeny / 4 x G; 2 x AG
Male progeny / 3 x A
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP185 (208) / ytP1071 / X
Female 1 / A
Female 2 / A
Male / G
Female progeny / 7 x AG
Male progeny / 4 x A
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP186(112) / ytP102 / X
Female 1 / A
Female 2 / G
Male / A
Female progeny / 12 x AG; 2x A
Male progeny / 5 x G; 1x A
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP187 (122) / ytP104 / X
Female / A
Male / T
Female progeny / 25 x AT
Male progeny / 6 x A
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP188 (281) / ytP1071 / X
Female / C
Male / T
Female progeny / 22 x CT
Male progeny / 7 x C
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP170 (231) / ytP33/ I
Female / A
Male / G
Female progeny / 5 x AG
Male progeny / 5 x AG
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP171 (185) / ytP68/ I
Female / AG
Male / G
Female progeny / 3 x AG
Male progeny / 3 x G
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP173 (330) / ytP117/ I
Female / A
Male / C
Female progeny / 10 x AC; 2 x A2
Male progeny / 5 x AC
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP176 (190) / ytP111/ I
Female / C
Male / CT
Female progeny / 8 x CT; 13 x C
Male progeny / 6 x CT; 9 x C
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP181 (205) / ytP891/ II
Female 1 / C
Female 2 / T
Male / CT
Female progeny / 1 x C; 7 x CT; 5 x T
Male progeny / 4 x CT; 2x T
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP182 (267) / ytP891/ II
Female 1 / CT
Female 2 / C
Male / T
Female progeny / 11 x CT
Male progeny / 5 x CT; 1 x T
Marker / Marker in S. ra. /chromosome / Worms / Genotype
ytP179 (331) / ytP108 / II
Female 1 / lost
Female 2 / AG
Male / AG
Female progeny / 1 x A; 6 x AG; 2 x G
Male progeny / 3 x A; 1 x AG; 1x G

One or two females were crossed with one male. The parents and free-living progeny were genotyped. Listed is the result of the sequencing reaction at the polymorphic position of the marker indicated. If two bases are present the individual was heterozygous, if only one base is present the individual was homo- or hemizygous. The two crosses shown in Table 2 are also included in this table. 1In some instances two new markers corresponding to the same S. ratti marker were made to test for consistancy. 2These two animals are unexpected and are the only two progenies we found in our crosses (shown and not shown) that appeared inconsistent with Mendelian inheritance of genetic markers in the free-living generation of P. trichosuri. Presumably these cases are due to an earlier mating of the female or to the erroneous transfer of eggs to the mating plates with the female.