Fink-Jensen A.
Supplemental material
Supplemental Table 1.
Parameter / M4+/+ / M4-/-Body weight / 26.6 g ± 0.7 / 26.7 g ± 0.5
Body length / 88.8 mm ± 0.8 / 89.7 mm ± 0.8
Body position / 4 (4 – 4) / 4 (3 - 4)
Spontaneous activity / 2 (1 – 2) / 2 (1 – 2)
Respiration / 2 (2 – 2) / 2 (2 – 2)
Tremor / 0 (0 – 0) / 0 (0 – 0)
Transfer arousal / 5 (3 – 5) / 5 (3 – 5)
Palpebral closure / 0 (0 – 0) / 0 (0 – 0)
Piloerection / 0 (0 – 0) / 0 (0 – 0)
Startle response / 1.5 (1 – 2) / 2 (1 – 3)
Gait / 0 (0 – 0) / 0 (0 – 0)
Pelvic elevation / 2 (2 – 2) / 2 (2 – 2)
Tail elevation / 1 (1 – 1) / 1 (1 – 1)
Touch-escape / 2 (1 – 2) / 2 (1 – 2)
Positional passivity / 0 (0 – 2) / 0 (0 – 1)
Trunk Curl / 0 (0 – 1) / 0 (0 – 1)
Limb grasping / 0 (0 – 0) / 0 (0 – 0)
Visual placing / 4 (4 – 4) / 4 (4 – 4)
Grip strength / 2 (1 – 2) / 2 (2 – 3)
Body tone / 1 (1 – 1) / 1 (1 – 1)
Pinna reflex / 1 (1 – 1) / 1 (1 – 1)
Corneal reflex / 1 (1 – 1) / 1 (1 – 1)
Toe pinch / 3 (2 – 3) / 3 (2 – 3)
Wire manoeuvre / 1 (1 – 3) / 1 (0 – 3)
Skin colour / 1 (1 – 1) / 1 (1 – 1)
Heart rate / 1 (1 – 1) / 1 (1 – 1)
Limb tone / 2 (2 – 2) / 2 (1 – 2)
Abdominal tone / 1 (1 – 1) / 1 (1 – 1)
Lacrimation / 1 (1 – 1) / 1 (1 – 1)
Salivation / 1 (1 – 1) / 1 (1 – 1)
Provoked biting / 1 (1 – 1) / 1 (1 – 1)
Righting reflex / 0 (0 – 0) / 0 (0 – 0)
Contact righting reflex / 1 (1 – 1) / 1 (1 – 1)
Negative geotaxis / 0 (0 – 0) / 0 (0 – 0)
Fear / 0 (0 – 1) / 0 (0 – 1)
Irritability / 0 (0 – 1) / 0 (0 – 0)
Aggression / 0 (0 – 1) / 0 (0 – 1)
Vocalisation / 0 (0 – 1) / 1 (0 – 1)
Locomotor activity / 4169 bb ± 429.2 (N=13) / 5342 bb ± 583.8 (N=14)
Supplemental Table 1. Behavioral phenotype assessment, primary screen (SHIRPA). Body weight, body length, and locomotor activity (bb: beam breaks) are group means ± SEM. Other data are group medians with range in parenthesis. Groups sizes: n=19-21. There was no significant effect of genotype in any of the measures.
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