Identification of Japanese Lymantria Species (Lepidoptera: Lymantriidae) Based On

Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on PCR-RFLPanalysis of mitochondrial DNA

Applied Entomology and Zoology

Makoto Arimoto1) and Ren Iwaizumi1)

1)Research Division, Yokohama Plant Protection Station, Yokohama, Kanagawa 231-0801, Japan

Corresponding author:

M. Arimoto

E-mail:

Present Address:

R. Iwaizumi

Inspection Operation Division, Yokohama Plant Protection Station, Yokohama, Kanagawa 231-0801, Japan

Supplementary Table 1 Details of the sources and collection dates of Lymantria spp.
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 9 / male / 25 Jul. 2004 / adult / Otaru, Hokkaido
58* / male / 26 Jul. 2004 / adult / Otaru, Hokkaido / AB839867
60 / male / 6 Aug. 2004 / adult / Otaru, Hokkaido
62 / male / 9 Aug. 2004 / adult / Otaru, Hokkaido
178 / male / 21 Jul. 2004 / adult / Otaru, Hokkaido
179 / male / 26 Jul. 2004 / adult / Otaru, Hokkaido
183 / male / 9 Aug. 2004 / adult / Otaru, Hokkaido
184 / male / 12 Aug. 2004 / adult / Otaru, Hokkaido
185 / male / 13 Aug. 2004 / adult / Otaru, Hokkaido
187 / male / 24 Aug. 2004 / adult / Otaru, Hokkaido
188 / male / 26 Aug. 2004 / adult / Otaru, Hokkaido
190 / male / 28 Aug. 2004 / adult / Otaru, Hokkaido
14 / male / 2 Aug. 2004 / adult / Sapporo, Hokkaido
15* / male / 17 Aug. 2005 / adult / Sapporo, Hokkaido / AB839868
63 / male / 3 Aug. 2004 / adult / Sapporo, Hokkaido
64 / male / 5 Aug. 2004 / adult / Sapporo, Hokkaido
65 / male / 6 Aug. 2004 / adult / Sapporo, Hokkaido
66 / male / 2 Aug. 2004 / adult / Sapporo, Hokkaido
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 67 / male / 14 Aug. 2005 / adult / Sapporo, Hokkaido
212 / male / 28 Jul. 2004 / adult / Sapporo, Hokkaido
213 / male / 30 Jul. 2004 / adult / Sapporo, Hokkaido
214 / male / 3 Aug. 2004 / adult / Sapporo, Hokkaido
217 / male / 4 Aug. 2004 / adult / Sapporo, Hokkaido
219 / male / 6 Aug. 2004 / adult / Sapporo, Hokkaido
220 / male / 6 Aug. 2004 / adult / Sapporo, Hokkaido
199 / female / 19 Aug. 2009 / adult / Tomakomai, Hokkaido
241* / male / 5 Aug. 2009 / adult / Tomakomai, Hokkaido / AB839869
49 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
50 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
51 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
52 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
53 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
54* / male / 18 Aug. 2010 / adult / Muroran, Hokkaido / AB839870
163 / female / 2010 / egg mass / Muroran, Hokkaido
164 / female / 2010 / egg mass / Muroran, Hokkaido
165 / female / 2010 / egg mass / Muroran, Hokkaido
166 / female / 2010 / egg mass / Muroran, Hokkaido
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 167 / female / 2010 / egg mass / Muroran, Hokkaido
168 / female / 2010 / egg mass / Muroran, Hokkaido
169 / female / 2010 / egg mass / Muroran, Hokkaido
170 / female / 2010 / egg mass / Muroran, Hokkaido
171 / female / 2010 / egg mass / Muroran, Hokkaido
172 / female / 2010 / egg mass / Muroran, Hokkaido
173 / female / 2010 / egg mass / Muroran, Hokkaido
174 / female / 2010 / egg mass / Muroran, Hokkaido
175 / female / 2010 / egg mass / Muroran, Hokkaido
201 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
202 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
203 / male / 18 Aug. 2010 / adult / Muroran, Hokkaido
204 / female / 18 Aug. 2010 / adult / Muroran, Hokkaido
205 / female / 18 Aug. 2010 / adult / Muroran, Hokkaido
206 / female / 18 Aug. 2010 / adult / Muroran, Hokkaido
207 / female / 18 Aug. 2010 / adult / Muroran, Hokkaido
68* / male / 2007 / adult / Hakodate, Hokkaido / AB839871
69 / male / 2007 / adult / Hakodate, Hokkaido
70 / male / 2007 / adult / Hakodate, Hokkaido
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 71 / male / 2007 / adult / Hakodate, Hokkaido
72 / male / 2007 / adult / Hakodate, Hokkaido
343 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
344 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
345 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
346 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
347 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
348 / male / 27 Jul. 2012 / adult / Ishinomaki, Miyagi
33 / male / 20 Aug. 2004 / adult / Rifu, Miyagi
34 / male / 19 Aug. 2004 / adult / Rifu, Miyagi
35 / male / 15 Aug. 2004 / adult / Rifu, Miyagi
24 / male / 22 Aug. 2005 / adult / Shiogama, Miyagi
144 / male / 5 Aug. 2005 / adult / Shiogama, Miyagi
145 / male / 8 Aug. 2005 / adult / Shiogama, Miyagi
146 / male / 11 Aug. 2005 / adult / Shiogama, Miyagi
147 / male / 18 Aug. 2005 / adult / Shiogama, Miyagi
140 / male / 28 Jul. 2005 / adult / Tagajo, Miyagi
141 / male / 31 Jul. 2005 / adult / Tagajo, Miyagi
142 / male / 1 Aug. 2005 / adult / Tagajo, Miyagi
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 143 / male / 3 Aug. 2005 / adult / Tagajo, Miyagi
256 / male / 29 Jul. 2011 / adult / Akita, Akita
257 / male / 29 Jul. 2011 / adult / Akita, Akita
258 / male / 29 Jul. 2011 / adult / Akita, Akita
259 / male / 29 Jul. 2011 / adult / Akita, Akita
260 / male / 29 Jul. 2011 / adult / Akita, Akita
354 / male / 18 Jul. 2012 / adult / Akita, Akita
355 / male / 18 Jul. 2012 / adult / Akita, Akita
356 / male / 1 Aug. 2012 / adult / Akita, Akita
357 / male / 1 Aug. 2012 / adult / Akita, Akita
358 / male / 15 Aug. 2012 / adult / Akita, Akita
359 / male / 23 Jul. 2012 / adult / Akita, Akita
360 / male / 23 Jul. 2012 / adult / Akita, Akita
361 / male / 1 Aug. 2012 / adult / Akita, Akita
362 / male / 7 Aug. 2012 / adult / Akita, Akita
285 / female / 9 Aug. 2011 / adult / Sakata, Yamagata
286 / male / 23 Aug. 2011 / adult / Sakata, Yamagata
238 / female / 29 Jul. 2011 / adult / Narita, Chiba
91 / male / 30 Nov. 2006 / egg mass / Chiba, Chiba
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 92 / male / 30 Nov. 2006 / egg mass / Chiba, Chiba
93 / male / 30 Nov. 2006 / egg mass / Chiba, Chiba
22 / male / 24 Jul. 2005 / adult / Yokohama, Kanagawa
23 / male / 6 Jul. 2005 / adult / Yokohama, Kanagawa
94 / male / 2 Jul. 2007 / adult / Yokohama, Kanagawa
95 / male / 5 Jul. 2007 / adult / Yokohama, Kanagawa
96 / male / 17 Jul. 2007 / adult / Yokohama, Kanagawa
161 / male / 16 Jul. 2005 / adult / Yokohama, Kanagawa
162 / male / 7 Jul. 2005 / adult / Yokohama, Kanagawa
176 / male / 10 Jul. 2005 / adult / Yokohama, Kanagawa
177 / male / 3 Jul. 2005 / adult / Yokohama, Kanagawa
11 / male / 1 Jul. 2004 / adult / Zushi, Kanagawa
152 / male / 3 Jul. 2004 / adult / Zushi, Kanagawa
153 / male / 2 Jul. 2004 / adult / Zushi, Kanagawa
154 / male / 1 Jul. 2004 / adult / Zushi, Kanagawa
155 / male / 1 Jul. 2004 / adult / Zushi, Kanagawa
25 / male / 2 Jul. 2004 / adult / Yokosuka, Kanagawa
156 / male / 19 Jun. 2004 / adult / Yokosuka, Kanagawa
157 / male / 29 Jun. 2004 / adult / Yokosuka, Kanagawa
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 158 / male / 1 Jul. 2004 / adult / Yokosuka, Kanagawa
159 / male / 2 Jul. 2004 / adult / Yokosuka, Kanagawa
160 / male / 6 Jul. 2004 / adult / Yokosuka, Kanagawa
292 / male / 21 May 2010 / adult / Niigata, Niigata
293 / female / 27 May 2009 / adult / Niigata, Niigata
287 / male / 2010 / adult / Jyoetsu, Niigata
288 / male / 2010 / adult / Jyoetsu, Niigata
289 / male / 2010 / adult / Jyoetsu, Niigata
290 / male / 2010 / adult / Jyoetsu, Niigata
106 / male / 9 Jul. 2004 / adult / Imizu, Toyama
107 / male / 23 Jul. 2004 / adult / Imizu, Toyama
108 / male / 9 Jul. 2004 / adult / Imizu, Toyama
10 / male / 29 Jul. 2004 / adult / Takaoka, Toyama
83 / male / 6 Aug. 2004 / adult / Takaoka, Toyama
84 / male / 6 Aug. 2004 / adult / Takaoka, Toyama
85 / male / 6 Aug. 2004 / adult / Takaoka, Toyama
86 / male / 13 Aug. 2004 / adult / Takaoka, Toyama
87 / male / 6 Aug. 2004 / adult / Takaoka, Toyama
17* / male / 8 Jul. 2005 / adult / Nagoya, Aichi / AB839872
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 18 / male / 30 Jun. 2005 / adult / Nagoya, Aichi
97 / male / 10 Jul. 2005 / adult / Nagoya, Aichi
98 / male / 13 Jul. 2005 / adult / Nagoya, Aichi
99 / male / 17 Jul. 2005 / adult / Nagoya, Aichi
223 / male / 5 Jul. 2005 / adult / Nagoya, Aichi
224 / male / 25 Jun. 2005 / adult / Nagoya, Aichi
225 / male / 8 Jul. 2005 / adult / Nagoya, Aichi
226 / male / 30 Jun. 2005 / adult / Nagoya, Aichi
227 / male / 2 Jul. 2005 / adult / Nagoya, Aichi
19 / male / 3 Jul. 2005 / adult / Okazaki, Aichi
109 / male / 27 Jul. 2005 / adult / Okazaki, Aichi
110 / male / 2 Jul. 2005 / adult / Okazaki, Aichi
111 / male / 3 Jul. 2005 / adult / Okazaki, Aichi
112 / male / 3 Jul. 2005 / adult / Okazaki, Aichi
113 / male / 2 Jul. 2005 / adult / Okazaki, Aichi
114 / male / 2 Jul. 2005 / adult / Okazaki, Aichi
36 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
37 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
38 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 100 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
101 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
102 / male / 17 Sep. 2007 / egg mass / Kobe, Hyogo
324 / female / 28 Mar. 2012 / egg mass / Nara, Nara
325 / female / 28 Mar. 2012 / egg mass / Nara, Nara
326 / female / 28 Mar. 2012 / egg mass / Nara, Nara
327 / female / 28 Mar. 2012 / egg mass / Nara, Nara
328 / female / 28 Mar. 2012 / egg mass / Nara, Nara
329 / female / 28 Mar. 2012 / egg mass / Nara, Nara
330 / female / 28 Mar. 2012 / egg mass / Nara, Nara
331 / male / 28 Mar. 2012 / egg mass / Nara, Nara
332 / male / 28 Mar. 2012 / egg mass / Nara, Nara
333 / male / 28 Mar. 2012 / egg mass / Nara, Nara
334 / male / 28 Mar. 2012 / egg mass / Nara, Nara
335 / male / 28 Mar. 2012 / egg mass / Nara, Nara
336 / male / 28 Mar. 2012 / egg mass / Nara, Nara
12 / male / 5 Jul. 2004 / adult / Hiroshima, Hiroshima
20 / male / 9 Jun. 2005 / adult / Hiroshima, Hiroshima
21 / male / 6 Jul. 2005 / adult / Hiroshima, Hiroshima
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 130 / male / 13 Jun. 2005 / adult / Hiroshima, Hiroshima
131 / male / 5 Jul. 2005 / adult / Hiroshima, Hiroshima
132 / male / 6 Jul. 2005 / adult / Hiroshima, Hiroshima
228 / male / 6 Jul. 2005 / adult / Hiroshima, Hiroshima
229 / male / 6 Jul. 2005 / adult / Hiroshima, Hiroshima
230 / male / 7 Jul. 2005 / adult / Hiroshima, Hiroshima
231 / male / 7 Jul. 2005 / adult / Hiroshima, Hiroshima
232 / male / 7 Jul. 2005 / adult / Hiroshima, Hiroshima
323 / male / 9 Jul. 1999 / adult / Tokushima, Tokushima
301 / male / 27 Jun. 2004 / adult / Matsuyama, Ehime
7 / male / 12 Jul. 2004 / adult / Kitakyushu, Fukuoka
73 / male / 12 Jul. 2004 / adult / Kitakyushu, Fukuoka
74 / male / 12 Jul. 2004 / adult / Kitakyushu, Fukuoka
75 / male / 14 Jul. 2004 / adult / Kitakyushu, Fukuoka
76 / male / 21 Jul. 2004 / adult / Kitakyushu, Fukuoka
77 / male / 21 Jul. 2004 / adult / Kitakyushu, Fukuoka
233 / male / 14 Jul. 2004 / adult / Kitakyushu, Fukuoka
234 / male / 12 Jul. 2004 / adult / Kitakyushu, Fukuoka
236 / male / 21 Jul. 2004 / adult / Kitakyushu, Fukuoka
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. dispar japonica / 237 / male / 12 Jul. 2004 / adult / Kitakyushu, Fukuoka
28 / male / 10 Jul. 2006 / adult / Oita, Oita
29 / male / 10 Jul. 2006 / adult / Oita, Oita
30 / male / 10 Jul. 2006 / adult / Oita, Oita
31 / male / 10 Jul. 2006 / adult / Oita, Oita
32 / male / 10 Jul. 2006 / adult / Oita, Oita
L. umbrosa / 59* / male / 16 Aug. 2004 / adult / Otaru, Hokkaido / AB839873
61 / male / 10 Aug. 2004 / adult / Otaru, Hokkaido
180 / male / 6 Aug. 2004 / adult / Otaru, Hokkaido
181 / male / 7 Aug. 2004 / adult / Otaru, Hokkaido
182 / male / 22 Aug. 2004 / adult / Otaru, Hokkaido
186 / male / 15 Aug. 2004 / adult / Otaru, Hokkaido
191 / male / 2 Aug. 2004 / adult / Otaru, Hokkaido
192 / male / 10 Aug. 2004 / adult / Otaru, Hokkaido
13* / male / 30 Jul. 2004 / adult / Sapporo, Hokkaido / AB839874
215 / male / 3 Aug. 2004 / adult / Sapporo, Hokkaido
216 / male / 4 Aug. 2004 / adult / Sapporo, Hokkaido
218 / male / 6 Aug. 2004 / adult / Sapporo, Hokkaido
221 / male / 6 Aug. 2004 / adult / Sapporo, Hokkaido
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. umbrosa / 43* / male / 10 Aug. 2010 / adult / Tomakomai, Hokkaido / AB839875
44 / male / 10 Aug. 2010 / adult / Tomakomai, Hokkaido
45 / male / 10 Aug. 2010 / adult / Tomakomai, Hokkaido
46 / male / 10 Aug. 2010 / adult / Tomakomai, Hokkaido
47 / male / 10 Aug. 2010 / adult / Tomakomai, Hokkaido
48 / male / 19 Aug. 2010 / adult / Tomakomai, Hokkaido
194 / female / 19 Aug. 2009 / adult / Tomakomai, Hokkaido
196 / female / 19 Aug. 2009 / adult / Tomakomai, Hokkaido
197 / female / 19 Aug. 2009 / adult / Tomakomai, Hokkaido
198 / female / 19 Aug. 2009 / adult / Tomakomai, Hokkaido
L. albescens / 137* / female / 20 May 2011 / 6th instar larva / Naha, Okinawa / AB839876
138 / female / 20 May 2011 / 6th instar larva / Naha, Okinawa
139 / female / 20 May 2011 / 6th instar larva / Naha, Okinawa
262 / male / 3 Jun. 2005 / adult / Naha, Okinawa
263 / male / 6 Jun. 2005 / adult / Naha, Okinawa
264 / male / 23 Jun. 2005 / adult / Naha, Okinawa
265 / male / 27 Jun. 2005 / adult / Naha, Okinawa
268 / male / 9 Jun. 2005 / adult / Naha, Okinawa
275 / male / 23 Jun. 2005 / adult / Naha, Okinawa
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. albescens / 276 / male / 27 Jun. 2005 / adult / Naha, Okinawa
277 / male / 27 Jun. 2005 / adult / Naha, Okinawa
278 / male / 30 Jun. 2005 / adult / Naha, Okinawa
279 / male / 6 Jun. 2005 / adult / Naha, Okinawa
280 / male / 6 Jun. 2005 / adult / Naha, Okinawa
281 / male / 9 Jun. 2005 / adult / Naha, Okinawa
282 / male / 7 Jun. 2005 / adult / Naha, Okinawa
283 / male / 6 Jun. 2005 / adult / Naha, Okinawa
284 / male / 9 Jun. 2005 / adult / Naha, Okinawa
L. postalba / 78 / male / 30 Jul. 2004 / adult / Tsushima, Nagasaki
79 / male / 30 Jul. 2004 / adult / Tsushima, Nagasaki
80* / male / 30 Jul. 2004 / adult / Tsushima, Nagasaki / AB839877
81 / male / 6 Aug. 2004 / adult / Tsushima, Nagasaki
L. xylina / 365 / male / 9 Jul. 1994 / adult / Tomiyama, Chiba
366 / male / 9 Jul. 1994 / adult / Tomiyama, Chiba
208 / female / 28 Jun. 2007 / adult / Oita, Oita
209 / female / 28 Jun. 2007 / adult / Oita, Oita
210 / male / 28 Jun. 2007 / adult / Oita, Oita
211 / male / 28 Jun. 2007 / adult / Oita, Oita
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. xylina / 115 / male / 20 May 2011 / pupa / Naha, Okinawa
116 / male / 20 May 2011 / pupa / Naha, Okinawa
117 / male / 20 May 2011 / pupa / Naha, Okinawa
118* / male / 20 May 2011 / pupa / Naha, Okinawa / AB839878
119 / male / 20 May 2011 / pupa / Naha, Okinawa
120 / male / 20 May 2011 / pupa / Naha, Okinawa
121 / male / 20 May 2011 / pupa / Naha, Okinawa
122 / female / 20 May 2011 / pupa / Naha, Okinawa
123 / female / 20 May 2011 / pupa / Naha, Okinawa
133 / female / 20 May 2011 / pupa / Naha, Okinawa
134 / female / 18 May 2011 / 6th instar larva / Naha, Okinawa
135 / female / 20 May 2011 / 6th instar larva / Naha, Okinawa
136 / male / 20 May 2011 / pupa / Naha, Okinawa
L. mathura / 124 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
125 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
126 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
127 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
128 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
129 / male / 20 Aug. 2009 / egg mass / Otaru, Hokkaido
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. mathura / 1 / female / 19 Aug. 2009 / egg mass / Tomakomai, Hokkaido
2 / female / 19 Aug. 2009 / egg mass / Tomakomai, Hokkaido
3 / female / 19 Aug. 2009 / egg mass / Tomakomai, Hokkaido
55* / female / 18 Aug. 2010 / adult / Tomakomai, Hokkaido / AB839879
56 / female / 19 Aug. 2010 / adult / Tomakomai, Hokkaido
57 / female / 19 Aug. 2010 / adult / Tomakomai, Hokkaido
243 / male / 5 Aug. 2009 / adult / Tomakomai, Hokkaido
244 / male / 5 Aug. 2009 / adult / Tomakomai, Hokkaido
245 / female / 5 Aug. 2009 / adult / Tomakomai, Hokkaido
246 / female / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
247 / female / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
248 / female / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
249 / male / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
250 / male / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
251 / male / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
252 / male / 6 Aug. 2009 / adult / Tomakomai, Hokkaido
350 / male / 8 Aug. 2008 / adult / Morioka, Iwate
349 / female / 23 Jun. 2012 / 6th instar larva / Yokohama, Kanagawa
254 / male / 22 Jul. 2005 / adult / Yokosuka, Kanagawa
Supplementary Table 1continued
Species / Voucher specimen No.a / Sex / Collection date / Collection stage / Locality (Japan) / Accession No.
L. mathura / 255 / male / 22 Jul. 2005 / adult / Yokosuka, Kanagawa
368 / male / 1 Aug. 2001 / adult / Kobuchizawa, Yamanashi
369 / male / 1 Aug. 2001 / adult / Kobuchizawa, Yamanashi
L. monacha / 150* / male / 19 Aug. 2009 / adult / Tomakomai, Hokkaido / AB839880
239 / female / 5 Aug, 2009 / adult / Tomakomai, Hokkaido
240 / male / 5 Aug. 2009 / adult / Tomakomai, Hokkaido
367 / male / 1 Aug. 2001 / adult / Kobuchizawa, Yamanashi
aAsterisks refer to specimens used for sequencing of mtDNA in the present study. All voucher speciemens are preserved at the Yokohama Plant Protection Station under the serial voucher specimen no.

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