Curriculum Vitae
Anzio Gartrell
Home Address
131 Sleepy Creek Drive
Athens, GA 30606
(706) 461-5672
E-mail:
Education
2013 Athens Technical College, Athens, GA
Associate Degree, Biotechnology
Biological Laboratory Technician Certificate
Regulatory and Compliance Technician Certificate
2013 Electron Microscopy Training, Veterinary School EM Lab and Barrow Hall EM
The University of Georgia, Athens, GA
2013 Work Ready Certification, Gold Level, Athens Technical College, Athens, GA
2011 & 2012 Georgia Fall Leadership Conference, Atlanta, GA
1989 Basic Electronics Certificate, South Fulton Vocational Center, College Park, GA
Skills Covered by Degree and Certificates
Biotechnology Associate Degree and Biological Laboratory Technician Certificates
General Laboratory Skills
Laboratory Notebook Maintenance, Use of Excel Spreadsheets for Graphing, Statistical Treatments of Data
Chemistry / Prep Techniques
Solution Preparation, Serial Dilutions, Molarity Calculations
Sterile Techniques, Sterile Media Preparation
Isolation and Transfer of Pure Microbiology Cultures
Use of pH and other Ion-selective Electrode Meters
Gravimetric & Titrimetric Methods of Analysis
Protein Techniques
Differential Centrifugation/Purification
Gel Filtration Chromatography
Purification of a Restriction Enzyme Using Ion Exchange Chromatography
Harvest, Fractionation, SDS-PAGE
Molecular Weight Determination
Bradford/Lowry Assays
Enzyme Assays Including Malate Dehydrogenase, β-Galactosidase, etc.
Immuno (Western) Blot
Enzyme-Linked Immunosorbent Assay (ELISA)
BioTek ELx800 Microplate Reader with GEN5 Software
Protein Chromatography Using an ÄKTAprime System and PrimeView Software
DNA Techniques
Media Preparations Including Selective and Special Indicator Media
Agarose Gel Electrophoresis for DNA Separation/Analysis
Plasmid/Chromosomal DNA Isolation, Restriction Analysis, Restriction Mapping, DNA Ligation
Staining/Destaining/Photo documentation & Analysis of DNA Gels
Conventional Transformation of E. coli, Electroporation
Construction of Recombinant Plasmid DNA Including Blue/White Cloning of DNA Fragments
PCR, Reverse Transcriptase-PCR, Southern Blot, Preparation of a Genomic Library
Instrumental Analysis Techniques
GC-FID & GC-MS - Method Setup, Standards Prep & Sample Analysis
HPLC -Method Setup, Mobile Phase Prep, Standards Prep & Sample Analysis
Flame AA - Digestions, Standards Prep & Sample Analysis
FTIR -Liquids, Gases, Mulls, Thin Films, Pellets
UV-Vis - Direct Use Of Spectrophotometer & Use As HPLC Detector
Recognition of Problems With All Above, Troubleshooting, and Correction of Minor Problems
Cell CultureTechniques
Preparation of Physiologically Active Chloroplasts & Isolation of Mitochondria, Marker Enzyme Assays
Isolation and Characterization of Plant Genomic DNA
Growth of Various Bacterial Strains In Liquid or Semi-Solid Media
Hybridoma Cell Culture Propagation in a Biological Safety Cabinet
Use of Bioreactors/Fermenters
Regulatory and Compliance Technician Certificate Reading/Writing Standard Operating Procedures (SOPs)
Validation/Calibration of pH Meters and Balances
TyvekTM suit gowning
Completion of GMP Documents, Eg. Batch Production Records
Perform Receiving, Quarantine and Release Operations
Clean, Decontaminate and Sanitize Production Equipment
Work Ready Certification
Reading for Information — measures the skills used when people read and use written text to do a job. Texts include memos, letters, directions, signs, notices, bulletins, policies, and regulations.
oThe test consists of multiple‐choice questions based on reading passages that reflect actual reading demands of the workplace.
oThe reading materials at level 3 are short and direct. The material becomes longer, denser and more difficult as readers move toward level 5.
Locating Information — measures the skills people use when they work with workplace graphics such as charts, graphs, tables, forms, flowcharts, diagrams, floor plans, maps, and instrument gauges.
o At level 3, the graphics are simple, and you will be asked to fill in missing information. At level 5, you will use the information in one or more complex graphics to draw conclusions and make decisions.
Applied Mathematics — measures skills used when applying mathematical reasoning and problem‐solving techniques to work‐related problems.
The formula sheet that includes all formulas required for the assessment is provided and you can use a calculator.
Basic Electronics Certificate
Electronic calculations applying Ohm’s law, reading and understanding of electrical schematic diagrams, using soldering iron to install and remove components of PC boards, testing for current, resistance, and voltage using Ohm meter
Work Experience and Job Responsibilities
2012-Present Research Scientist, Hajduk Laboratory BMB, The University of Georgia
Culturing Trypanosomes for experimental probing, data collection using modern
Biotechnology techniques, interpretation of collected data, lab tech
2011-2015 Microbiology and English Tutor, Athens Technical College
Duties included tutoring students in the intricacies of Biology, preparing students for tests, and assisting students with English essays and reports.
2010 -2011 Laboratory Technician, Athens Technical College
Duties included autoclaving waste materials, washing and sterilizing glassware, creating MSDS archives, maintaining routine lab procedures, preparing bacterial cell cultures, inoculating media with cell cultures, mixing and pouring media, and
2007-2010 Warehouse Manager, Bolton Dining Common, The University of Georgia
Responsible for ordering, receiving, inventorying, and distributing all dining hall products, training of all student and full time employees that worked in warehouse. I was responsible for calculating and inputting daily expense records using the Food Pro program. Checking temperatures, recording information and maintaining all freezers, coolers and the warehouse area.
2005-2007 Boxing Instructor/Personal Trainer, Self-Employed
Instruction in the science of boxing, proper form, workout, and eating habits.
2004-2005 Crew Supervisor, Fred Mormon Building, Athens, GA
I was responsible for supervising a team of painters, painting and renovating The Fred Building (formerly the Commerce building) of downtown Athens.
2003-2004 Criminal Investigator, Green and Green Attorneys-at-Law, Athens, GA
Interviewed clients, investigated crime scenes, gathered witness testimonies, served subpoenas, and researched criminal backgrounds.
1997-2003 Driver/Landscaper Kesler Irrigation and Landscaping, Winterville, GA
Interviewed clients, investigated crime scenes, gathered witness testimonies, served subpoenas, and researched criminal backgrounds.
1995-1997 Maintenance Technician, Wheat Street Garden Apartments, Atlanta, GA
I was responsible for all apartment maintenance, appliance repair, and replacement. Manufacturing of barrier and screen doors, light plumbing and electrical repair
1989-1995 D.J.s R US, Self-Employed, Atlanta, GA
Marketing, Advertising, Scouting of potential event locations, Fee Negotiations
1985-1989 Stock Clerk, Winn-Dixie, College Park, GA
Inventorying my department, stocking of product, ordering and check off of incoming product
Honors/Awards
1995 Presented Plaque for Community Service by East Athens Community Center
20112012 Selected for Georgia Fall Leadership Program
Honored by Athens Rotary Club for Most Money Raised by Individual for
Plungefest Fundraising Event
Honored by Athens Rotary Club for Most Money Raised by a Team for
Plungefest Fundraising Event
2014 Nominated for Campus Service Award, to be given on April 11, 2014
This award is the highest award the college gives for service through clubs and leadership among students.
Administrative and Student Organizations Positions
2010 Served as Panel Judge for Miss Black Athens pageant
2011-2013 Served as President and Vice President of Athens Tech ROTORACT
Took club from small inactive group to a 25+ group of extremely active students
Directed and performed personally a beautification project at Ralph Stephens
Community Visitation Center
Led group of student members in landscaping of Athens Rotary Club’s Habitat for
Humanity Project
2012-2013 Served as President of Athens Tech’s International Club
Organized club as a founding member
Directed projects for the club including International Day and Spring Fling
2012 Served as Treasurer for Athens Tech’s Biotechnology Club
Helped in decision making
Made certain books were balanced and money was handled properly
2011-2013 Nominated and served as a Student Ambassador for Athens Tech
Served as greeter for the college with dignitaries from the Governor’s
office, State Senators, and State Representatives
Worked at reception desk to welcome Caterpillar executives
Helped manage schedule of interviews for Caterpillar potential employees
Gave future students and potential employees tours of the campus
Worked as greeter for Honor’s Day ceremonies
Given special recognition for my efforts by Dr. Flora Tydings, President
of Athens Technical College
2013-2014 Served as a Board Member of Athens Tech’s Alumni Association
Assisted in fundraising
Served as a greeter at Fall Alumni Car Show
2013-2015 Served as a advisor for intensifying the Biotechnology Program at Athens Tech
Community Services
2013- Present Head Football Coach for Stonehenge Youth Association 4-6 yrs old
2012- Present Co-coordinator for the annual Athens Rotary sponsored Polar Bear
Plunge Fundraiser at Sandy Creek Park and Recreation Center
2012-Present Salvation Army Bell Ringer serving at both East and Westside
Wal-marts
2011-Present Assist Judge Barron in the annual giving of dictionaries to 3rd graders at Stroud
Elementary School
Special Training: Protein Purification and Electron Microscopy
2013 Purification of TLF, Apol-1, and Hpr from Nurse Assisted Human Blood Draw
§ Separation of RBCs from Serum by High Speed Centrifugation
§ Separation of LDLs and HDLs in Serum by use of Heavy and Light Salt Solutions and High Speed Centrifugation
§ Purification of TLF, Apol-1, and Hpr from HDL by use of Antibody Column Chromatography
§ Bradford Standard Curve, Graph, and Protein Concentration Calculation
§ SDS PAIGE GEL for visual and size confirmation
§ Western BLOT for antibody specific positive and negative controls
2016 E.coli Transformation, Expression, and Purification of 10x His tagged JBP-1
§ Transform E.coli with special tagged JBP-1 pDNA
§ Grow transformed E.coli on LB AMP selective plates for colony selection
§ Inoculate LB Amp media with single colony E.coli
§ Separate cells from media by High Speed Centrifugation
§ Lyse cell separate debree from supernatant by High Speed Centrifugation
§ Purify JBP-1 by use of Talon Resin Ion Chromatography
§ Bradford Standard Curve, Graph, and Protein Concentration Calculation
§ SDS PAIGE GEL for visual and size confirmation
§ Western BLOT for antibody specific positive and negative controls
Electron Microscopy
§ TEM /SEM microscopy chemical and procedure prep for cell fixation.
§ Metal infusion for making electron dense.
§ Dehydration, resin infusion, block facing and sectioning.
§ TEM/SEM loading and operations procedure for producing sections and negative stain micrographs
Publications
“Extracellular Vesicles from Trypanosoma brucei Mediate Virulence Factor Transfer and Cause Host Anemia. “. Cell 2016 Jan. http://www.pubfacts.com/detail/26771494/Extracellular-Vesicles-from-Trypanosoma-brucei-Mediate-Virulence-Factor-Transfer-and-Cause-Host-Anem
Acknowledgements for Electron Microscopy and Apol-1 purification
The krebs cycle enzyme α-ketoglutarate decarboxylase is an essential glycosomal protein in bloodstream African trypanosomes.” Eukaryot Cell 2015 Mar 21. http://www.pubfacts.com/detail/25416237/The-krebs-cycle-enzyme-ketoglutarate-decarboxylase-is-an-essential-glycosomal-protein-in-bloodstream
“Trypanosome Lytic Factor-1 Initiates Oxidation-stimulated Osmotic Lysis of Trypanosoma brucei brucei.”
References
Dr. Michael Mouat, Biotechnology Instructor, Athens Technical College (706) 355-5177Dr. Jeff Rapp, Biotechnology Program Chair, Athens Technical College (706) 355-5086
Dr. Heltzel, Biotechnology and Microbiology Instructor Athens Technical College (706) 355-5138
Dr. Hajduk Life Science Building UGA (706) 542-1676