Pathology Ch27 -- Peripheral Nerves and Skeletal Muscle -- PARTIAL pp1227-1235 (Peripheral Nerves)

Disease of Peripheral Nerves

·  Somatic Motor Function

1.  Lower motor neuron located in anterior horn of the spinal cord or in the brainstem

2.  Axon that travels to target muscel as aprt of a nerve

3.  Neuromuscular junctions

4.  Multiple innervated myofiber (muscle fibers)

·  Somatic Sensory Function

1.  Distal nerve endings, may contain specialized structures that register specific sensory modalities

2.  Axon that travels as part of a peripheral nerve to the dorsal root ganglia

3.  Proximal axon segment that synapses on neurons in the spinal cord or brain stem

·  Sensations and Motor Signals

o  Axons can be distinguished based on their diameter (correlated to myelin sheath thickness and conduction speeds)

§  Thin unmyelinated fibers > autonomic functions + pain and temperature sensation

§  Larger diameter axons w/ thick sheaths > light touch and motor signals

o  Schwann cells > make one myelin sheath create "internode" segment, separated by "nodes of Ranvier"

o  Axons bundled by 3 CT components:

§  Epineurium: encloses entire nerve

§  Perineurium: multilayered concentric sheath that groups subsets of axons into fascicles

§  Endoneurium: surrounds individual nerve fibers

·  General Types of Peripheral Nerve Injury

o  Axonal Neuropathies (axons are primary targets of damage)

§  Morphological hallmark: Wallerian degeneration, produced experimentally via transsection

·  Distal to transsection > disconnected from CNS and degenerate within days

o  Macrophages remove axonal and myelin debris

o  Distal myofibrils atrophy

·  Regeneration starts at proximal site of transecretion w/ formation of growth cone

o  Schwann cells and baement membranes guide the sprouting axons > grow 1mm/day

o  Continuous pruning removes misguided axon branches

o  Schwann cells create new myelin sheath, but internodes tend to be thinner/shorter

·  Successful only if transsected ends remain in close proximetry

o  If axons can't find distal target > produce psuedotumor aka traumatic neuroma

o  > whorled proliferation of axonal processes and Schwann cells > painful nodule

§  In vivo: degenerating and regenerating axons coexist > damage outpaces repair > progressive axon loss

§  Electrophysiologic: reduction in signal strength

o  Demyelinating Neuropathies (Schwann cells are the primary targets of damage)

§  Individual myelin sheaths degenerate in random pattern > discontinuous damage

§  Schwann cells proliferate and initiate repair > again, shorter and thinner than before

§  Electrophysiologic: slowed nerve conduction velocity

o  Neuronopathies (destruction of neurons > secondary degeneration of axonal processes)

§  Infections (herpes zoster) and toxins (platinum compounds) are examples of insults

§  Damage at neuronal cell body > peripheral nerve dysfxn equal in proximal & distal parts of the body

·  Anatomic Patterns of Peripheral Neuropathies

o  Mononeuropathies: affect a single nerve > deficits in restricted distribution

o  Polyneuropathies: affect multiple nerves (usually symmetrically) > deficits start in feet and ascend w/ progression

§  Hands begin by the time disease progressed to knees > "stocking and glove" distribution of sensory deficit

o  Mononeuritis multiplex: affects several nerves haphazardly > ex. L wrist drop and R foot drop

o  Polyradiculoneuropathies: affect nerve roots + peripheral nerves > diffuse symmetric symptoms (proximal + distal)

·  Specific Peripheral Neuropathies

o  Inflammatory Neuropathies

§  Guillain-Barre Syndrome (Acute Inflammatory Demyelinating Polyneuropathy)

·  Pathogenesis: acute-onset immune-mediated demyelinating neurpathy

o  2/3 cases preceded by acute flu-like illness

o  T-cell-mediated immune response > segmental demyelination via active macrophages

·  Morphology: inflammation and demyelination of spinal nerve roots and peripheral nerves

·  Clinical features: ascending paralysis (distal muscles > proximal muscles) and areflexia

o  Complication leads to death: respiratory paralysis, autonomic instability, cardiac arrest

§  Chronic Inflammatory Demyelinating Poly(radiculo)neuropathy

·  Most common chronic acquired inflammatory peripheral neuropathy

·  Pathogenesis: T cells and humoral factors implicated in inflammatory process

o  Molecules at Schwann cell-axon juction targetted by immune response

o  Complement-fixing IgG and IgM found on myelin sheath > recruit macrophages

·  Morphology: repeat demyelination and remyelination > "onion-bulb" structures

·  Clinical features: symmetrical mixed sensorimotor polyneuropathy, persists for 2+ months

§  Neuropathy Associated w/ Systemic Autoimmune Disease

·  Associated w/ rheumatoid arthritis, Sjogren syndrome, systemic lupus erythematosus (SLE)

·  Can manifest as distal sensory or sensorimotor polyneuropathies

§  Neuropathy Associated w/ Vasculitis (noninfectious inflammation of blood vessels)

·  1/3 of pt w/ vasculitis have peripheral nerve involvement and neuropathy

·  Often presents as mononeuritis multiplex (can be mononeuritis or polyneuropathy)

·  Peripheral nerves show patchy axonal degeneration and loss

·  Perivascular inflammatory infiltrates often present

o  Infectious Neuropathies

§  Leprosy (Hansen Disease)

·  Lepromatous leprosy

o  Schwann cells invaded by mycobacterium leprae

o  Demyelination and remyelination and loss of axons

o  Progresses to endoneurial fibrosis and multilayered thickening of perineurial sheath

o  Develop symmetric polyneuropathy (most severe in cool distal extremities and in face)

o  Infection prominently involves pain fibers > loss of pain sensation

·  Tuberculoid leprosy

o  Active cell-mediated immuner esponse to M. leprae

o  Manifest as dermal nodules containing granulomatous inflammation

o  Injures cutaneous nerves in the vicinity

o  Axons, Schwann cells, myelin are lost > fibrosis of perineurium and endoneurium

o  Much more localized nerve involvement

§  Lyme Disease

·  Neurologic manifestations in second and third stages of disease

·  Polyradiculoneuropathy and unilateral or bilateral facial nerve palsies

§  HIV/AIDS

·  HIV > peripheral neuropathy due to immune dysregulation

·  Early stage = mononeuritis multiplex and demyelinating disorders or chronic inflammatory demyelinating polyradiculoneuropathy

·  Later stages = distal sensory neuropathy (often painful)

§  Diphtheria

·  Diphthernia exotoxin produces acute peripheral neuropathy

·  Associated w/ prominent bulbar and respiratory muscle dysfunction > disability/death

§  Varicella-Zoster Virus

·  One of the most common viral infections of the PNS

·  Following chickenpox, latent infection persists within neurons of sensory ganglia

·  If reactivated later on > transported along sensory nerves to skin > infects keratinocytes

·  Painful, vesicular skin eruption (shingles) in distribution that follows sensory dermatomes

·  Decreased cell-mediated immunity though to play a role in reactivation

o  Metabolic, Hormonal, and Nutritional Neuropathies

§  Diabetes

·  Most common cause of peripheral neuropathy

·  Prevalence depends on duration of disease (50% overall, 80% of those w/ 15 years)

·  Pathogenesis: metabolic and vascular changes contribute to damage of neurons/Schwann cells

o  Nonenzymatic glycosylation > advanced glycosylation end products (AGEs) > interfere w/ normal protein function > activate inflammatory signaling

·  Morphology: axonal neuropathy

·  Clinical features: ascending distal symmetric sensorimotor polyneuropathy MOST COMMON

o  Numbness, loss of pain sensation, difficulty w/ balance, paresthesias or dysesthesias

o  Can also cause dysfunction of the ANS > postural hypotension, incomplete bladder emptying, sexual dysfunction

§  Other Metabolic, Hormonal, and Nutritional Neuropathies

·  Uremic neuropathy: most renal failure pts have peripheral neuropathy, recover after dialysis

·  Thyroid dysfunction: hypothyroidism > compression mononeuropathies or distal symmetric sensory polyneuropathy

·  Vitamin B12 (cyanocobalamin) deficiency: subacute degeneration and damage to long tracts in spinal cord and peripheral nerves

·  Deficiencies of vitamin B1 (thiamine), B6 (pyridoxine), folate, vitamin E, copper, and zinc

o  Toxic Neuropathies

§  After exposure to industrial or environmental chemicals, biologic toxins, or chemotherapeutic drugs

§  Alcohol, heavy metals (lead, mercury, arsenic, thallium), and organic solvents

o  Neuropathies Associated w/ Malignancy

§  Direct infiltration or compression of peripheral nerves by tumor > brachial plexopathy (apex of lung), obturator palsy (pelvic neoplasms), cranial nerve palsies (intracranial tumors), polyradiculopathy of lower extremities (cauda equina infiltrated)

§  Radiation, poor nutrition and infection, along w/ chemotherapy damage nerves

§  Paraneuropastlic neuropathies > sensorimotor neuronopathy is the most common (small cell lung cancer)

§  Neuropatheis associated w/ monoclonal gammopathies > neuplastic B cells secrete monoclonal immunoglobulins (IgM, IgG, IgA) > damage nerves

o  Neuropathies Caused by Physical Forces

§  Lacerations > cutting injuries or sharp fragments of fractured bone

§  Avulsion > results of tension being applied, often to one of the limbs

§  Compression (entrapment) neuropathy > peripheral nerve chronically subjected to increased pressure

·  Ex. carpal tunnel syndrome

·  "Saturday night palsy" = sleeping w/ arm in awkward position

o  Inherited Peripheral Neuropathies

§  Group of genetically diverse disorders w/ overlapping clinical phenotypes that often present in adults

§  Subsets of involved genes grouped based on coding for the following:

·  Myelin-associated proteins

·  Growth factors and growth factor receptors

·  Proteins that regulate mitochondrial function

·  Proteins that are involved in vesicle and axonal transport

·  Heart shock proteins, which may prevent protein aggregation

·  Proteins that are involved in cell membrane structure or function

§  Hereditary motor and sensory neuropathies (Charcot-Marie-Tooth disease)**

·  Distal muscle atrophy, sensory loss, and foot deformities

·  CMT1: duplication of region on c17 for peripheral myelin protein 22 (PMP22) gene

·  CMTX: mutation in GJB1 gene (encodes connexin32, a gap junction component in Schwann cells)

·  CMT2: associated w/ axonal rather than demyelinating injury > MFN2 gene mutation

§  Hereditary sensory neuropathies, w/ or w/o autonomic neuropathy

·  Typically axonal neuropathies

·  Loss of sensation (pain and temperature most common) and variable autonomic disturbances

§  Hereditary neuropathy w/ pressure palsy

·  Caused by deletion of PMP22 gene

·  Transient motor and sensory mononeuropathies, triggered by compression

·  Symptoms usually resolve within days/weeks, but some progress to chronic disease

·  Morphology: swollen, bulbous myelin sheaths at end of internodes ("tomaculi")

§  Familial amyloid polyneuropathies

·  Germ line mtuation of transthyretin gene

·  Amyloid deposition within peripheral nerves

§  Peripheral neuropathy accompanying inherited metabolic disorders

·  Leukodystrophies (ex. adrenoleukodystrophy, porphyria, Refsum disease)