Primers pairs to generate the Cµ-Cg3 FISF probe :
MG1F : CCTCACCAAGTCCACCAAGT
MG1R : GGTACGCAAGGTCATCTGGT
MG2F : TGACCCACCCAGACTACACA
MG2R : AGCAAGTCTACCAGGCTCCA
MG3F : TGGAGCCTGGTAGACTTGCT
MG3R: CATCCTGCAGTGAGGAGACA
MG4F : AGGCCATTGCAACCTTACAC
MG4R : CCACAGGAGCTGGAAGAGTC
MG5F : GAACTTCTGCTGGGCTATGC
MG5R : CAGAGGAGCAACCTCCTGAC
MG6F : TGACTGTTTCCTTGCTGTGC
MG6R : TGGGAGGTATTGGGGTCATA
MG7F : TAGGCTGCTCACCTTGGTCT
MG7R : GAAGCAGCACCATCTTCACA.
Primers binding at the first exons of the IGH constant genes :
Cµ : ACTTCCTTCCCGACTCCATC
Cd : GAGAGCTGGCTGCTTGTCAT
Cg : CTGAGTTCCACGACACCGTCA (consensus, at the four constant γ genes)
Ca1 :ATCTGGCTGGGTGCTGCAGAGGCT
Ca2 : ATCCTGGCTAGGTGGGAAGT
Ce : TAGTGACCAGAGAGCGTGAGG
Primers binding at the Switch regions :
SµF: CTTCTGGTTGTGAAGAGGTGGTTTTG
SµREV : GACTTAGACAAGGGCGATGC
Seq : TGCTGAAGACAGGACTGTGG