Illustration 1: Table 1 (a and b): Grade, Stage and DNA Ploidy

1a Grade and ploidy

Aneuploid (hyper-diploid) / Diploid (Normal) / Totals
Carcinoma in Situ / 1 / 0 / 1
Grade 1 / 3 (25%) / 9 / 12
Grade 2 / 6 (66.7%) / 3 / 9
Grade 3 / 8 (66.7%) / 4 / 12
Totals / 18 / 16 / 34

1b: Stage and ploidy

Aneuploid (Hyper-diploid) / Diploid (Normal) / Totals
Cis+pTa+pT1 tumours / 9 (39%) / 14 / 23
T2 to T4 tumours / 9 (82%) / 2 / 11
Totals / 18 (53%) / 16 / 34

Illustration 2: Table 2 (a and b): DNA ploidy and outcome of superficial (cis+pTa+pT1) and muscle-invasive (T2 to T4) tumours

2a: Superficial (cis+pTa+pT1) tumours

Outcome / Aneuploid / Diploid
No recurrence or recurrence of same grade and stage / 4 / 9
Recurrence of higher grade (same stage) / 0 / 1
Recurrence of higher stage / 3 / 0
Died of unrelated cause / 0 / 1
Totals / 7 / 11

2b: Muscle-invasive tumours

Aneuploid / Diploid
Alive and well / 6 (2 were originally superficial and progressed) / 0
Died due to tumour / 3 / 3
Tumour progressed / 1 / 0
Died of unrelated cause / 0 / 1
Totals / 10 / 4

Illustration 3: Table 3 (a and b): Expression of p53 with regard to Grade and Stage

3a: Expression of p53 and Grade

Grade / p53+ve / p53-ve / Totals
Grade 1 / 5 / 5 / 10
Grade 2 / 7 / 2 / 9
Grade 3 / 8 / 3 / 11
Totals / 20 / 10 / 30

3b: Expression of p53 and stage

p53+ve / p53-ve / Totals
Cis+pTa+pT1 (superficial tumours) / 15 / 5 / 20
T2 to T4 (muscle-invasive tumours) / 5 / 5 / 10
Totals / 20 / 10 / 30

Illustration 4: Table 4 (a and b):

Expression of p53 and outcome of superficial (cis+pTa+pT1) and muscle-invasive (T2 to T4) tumours reviewed.

4a:

Expression of p53 and outcome of superficial (cis+pTa+pT1) tumours reviewed.

Staining characteristics / No recurrence orrecurrence of same grade / Recurrence of higher grade / Recurrence of higher stage / Died of unrelated cause
P53 moderate/strongly positive / 6 / 1 (from G1 to G3) / 3 / 1
Weak p53 positive / 2 / 0 / 0 / 0
p53 negative / 3 / 0 / 0 / 0
Totals / 11 / 1 / 3 / 1

4b:

Outcome of muscle-invasive tumours reviewed.

Staining characteristics / Alive and well no tumour / Tumour progressed / Died of tumour / Died of unrelated cause
p53 moderately/strongly positive / 3 (1 was superficial earlier) / 0 / 2 / 1
Weak p53 positive / 1 (was superficial earlier) / 0 / 0 / 0
p53 negative / 2 / 1 / 3 / 0
Totals / 6 / 1 / 5 / 1

Illustration 5: Table 5 (a, b and c)

DNA ploidy and expression of p53 in human urothelial carcinoma with regard to grade and stage

5a: All Grades and stage

Aneuploid & p53 positive / Aneuploid and p53 negative / Diploid & p53 positive / Diploid & p53 negative / Total
Number / 11 / 5 / 9 / 5 / 30

5b: Stage

Aneuploid & p53 positive / Aneuploid and p53 negative / Diploid & p53 positive / Diploid & p53 negative / Total
Cis + pTa + pT1 tumours / 6 / 2 / 9 / 3 / 20
T2 to T4 tumours / 5 / 3 / 0 / 2 / 10
Totals / 11 / 5 / 9 / 5 / 30

5c: Grade

Aneuploid & p53 positive / Aneuploid and p53 negative / Diploid & p53 positive / Diploid & p53 negative / Total
Grade 1 / 0 / 2 / 5 / 3 / 10
Grade 2 / 5 / 1 / 2 / 1 / 9
Grade 3 / 6 / 2 / 2 / 1 / 11
Totals / 11 / 5 / 9 / 5 / 30

Illustration 6: Table 6 (a and b):

Outcome of superficial (cis + pTa + pT1) tumours and muscle-invasive tumours with regard to their DNA ploidy and expression of p53

6a: Outcome of superficial (cis+pTa+pT1) tumours reviewed.

DNA ploidy and staining characteristics / No recurrence or recurrence of same grade and stage / Recurrence of higher grade and same stage / Recurrence of higher stage / Died of unrelated cause
Aneuploid&p53 moderate/
strongly positive / 2 / 0 / 3 / 1
Aneuploid & weak p53 positive / 1 / 0 / 0 / 0
Aneuploid & p53 negative / 0 / 0 / 0 / 0
Diploid&moderate/strong
p53 positive / 4 / 1 (from G1 to G3) / 0 / 0
Diploid&weak p53 positive / 1 / 0 / 0 / 0
Diploid & p53 negative / 3 / 0 / 0 / 0
Totals / 11 / 1 / 3 / 1

6b: Outcome of muscle-invasive (T2 to T4) tumours reviewed.

DNA ploidy and staining characteristics / Alive and well no tumour / Tumour progressed / Died of tumour / Died of unrelated cause
Aneuploid & p53 moderate/strongly positive / 3 (1 was superficial earlier) / 0 / 2 / 0
Aneuploid & weak p53 positive / 1 (was superficial earlier) / 0 / 0 / 0
Aneuploid & p53 negative / 2 / 1 / 1 / 0
Diploid & moderate /strong p53 positive / 0 / 0 / 0 / 1
Diploid & weak p53 positive / 0 / 0 / 0 / 0
Diploid and p53 negative / 0 / 0 / 2 / 0
Totals / 6 / 1 / 5 / 1

Illustration 7: Figure 1. Bladder tumour strongly positively stained for p53

Illustration 8: Figure 2. Bladder tumour moderately positively stained for p53

Illustration 9: Figure 3. Flow Cytometer

Illustration 10: Figure 4. A Diploid Tracing from a carcinoma of the urinary bladder

Illustration 11: Figure 5: A Hyper-diploid (aneuploid) tracing a carcinoma of the urinary bladder