Primer / Direction a / Sequence (5’ → 3’) / Tm b (°C)
All Prevotella (genus specific) / Forward / CCAGCCAAGTAGCGTGCA / 58.1
Reverse / TGGACCTTCCGTATTACCGC / 58.5
Prevotella sp. P4P_62 (Alloprevotella rava) (P1, HOT 302)
/ Forward / TGTGATGAGGCATCTTGTTGC / 59.5
/ Reverse / ACTTAATCGTCCGCCTACGCT / 59.9
P. tannerae (Alloprevotella tannerae) (P2, HOT 466)
/ Forward / TACCGAACCTGCCCATCAC / 59.1
/ Reverse / TATACTCCCGCCTGCGCT / 59.2
P. oralis (P3, HOT 705)
/ Forward / TTCCCATTACTACGGCATACCC / 59.7
/ Reverse / CCGCCTGCTTACTGCGTAC / 59.3
P. buccae (P4, HOT 560) / Forward / TCCTCCTTTGAAGGCATCTGA / 59.6
Reverse / GTTGGGCCGCTGCTTTT / 59.6
Prevotella sp. BR014 (P5, HOT 300)
/ Reverse / CGCACTCAAGCAGGACAGTTT / 59.1
P. intermedia (P6, HOT 643) / Forward / TGTTGTCCACATATGGCATCTG / 59.1
Reverse / GCATACGTTGCGTGCACTC / 58.7
P. nigrescens (P7, HOT 693) / Reverse / AGGAAGCCCACGTCTCTGT / 55.9
P. multiformis (P8, HOT 685) / Forward / TGACGGCATCAGAGGTGAA / 58.5
Reverse / GCGCTGCACATCAGACGT / 59.3
P. salivae (P9, HOT 307) / Forward / TGAGCTACGTGTAGTTTATTGCAGG / 59.6
Reverse / GCTAATTCCTAAGTGAACTAACAACGG / 59.7
P. pleuriditis (P10, HOT 303) / Forward / AGCATTTGCGGTGATGGAT / 58.7
Reverse / GTTACCGGTTGAGCCGGTAC / 59.1
Prevotella sp. DA058 (P11, HOT 304) / Forward / ATGTTATCGAAAGCTCTCATGAGACTT / 59.8
Reverse / GCAATTCAGATGTTGAGCATCTACA / 60.3
Prevotella sp. P4PB_24 (P12, HOT 443) / Forward / TACTCCATGGTTTCCGCTGA / 58.9
Reverse / CATTCAAAAGAACGAAGAACGG / 58.6
P. denticola (P13, HOT 291) / Forward / ACGGCATCAGATTCGAAGC / 58.6
Reverse / CACACTTAATCCCCGGCCTA / 59.6
P. maculosa (P14, HOT 289) / Forward / TCGTCGGAAGTCATCTGATGA / 59.0
P. melaninogenica (P15, HOT 469) / Forward / ACTAATACCGCATAGTCTTCGATGAC / 58.9
P. veroralis (P16, HOT 572) / Forward / TGTAATTCTTAGATGACATCAGAAGAGAA / 58.0
P. oulorum (P17, HOT 288) / Forward / AGAGCTTGCTCTCTTAGGCGT / 57.5
P. oris (P18, HOT 311) / Forward / AAAGATTTCATCGGTCAGGGAT / 58.6
P. loescheii (P19, HOT 658) / Forward / TTGCTTGCAACTCCCGAT / 57.5
Prevotella sp. P4P_53 (P20, HOT 526) / Forward / ATCATGCGGATTGCTTGC / 57.9
Prevotella sp. B31FD (P21, HOT 317) / Reverse / CTTTCGGCGCACTCAAGC / 59.3
Prevotella sp. BE073 (P. histicola) (P22, HOT 298) / Forward / CCTTCCCATGACTAAGGGATAA / 58.4

* [10]

a Primers with no forward or reverse primer pairs were used with either Prevotella genus specific reverse or forward primer respectively.

b The melting temperature of DNA (Tm) was determined using Primer Express Version 1.0 (Applied Biosystems)

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