Electronic Supplementary Material

A study on the direct electrochemistry and electrocatalysis of microperoxidase-11 immobilized on a porous network-like gold film: Sensing of hydrogen peroxide

Qian-Li Zhang,a,b Ai-Jun Wang,a Zi-Yan-Meng,a Ya-Hui Lu,c Hong-Jun Lin,a Jiu-Ju Fenga*

aCollege of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China

bSchool of Chemistry and Biological Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China

cSchool of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China

*Corresponding author: , Tel./Fax: +86 579 82282269

Fig. S1. The CVs of the porous network-like Au film modified electrode in 0.5 M H2SO4 solution. Scan rate: 100 mV·s–1.

Fig. S2(A):Typical CVson theMP-11/cysteamine/Cu@Au/GCEat different scan rates(curve a-h): 10, 20, 30, 40, 50, 60, 70,and 100 mV·s–1in 20mM phosphate solution(pH7.0).(B): The oxidation and reduction currentsvs. scan rates.

Fig.S3Typical CVson the MP-11/cysteamine/Cu/GCE electrode with different scan rates(curve a-h): 10, 20, 30, 40, 50, 60, 70, and 100mV·s–1 in 20mM phosphate solution(pH7.0).

Fig.S4 (A) The oxidation and reduction currents vs. pH. (B) The oxidation and reduction potentials vs. pH.

Fig.S5Typical CVstowardsthe catalytic reduction of H2O2onthe MP-11/cysteamine/Cu@Au/GCE in 20mM N2 saturated phosphate solution with different concentrations of H2O2(curve a-f): 0, 0.010, 0.019, 0.039, 0.058, and 0.078mM. Scan rate: 100mV·s–1.

Fig.S6Typical CVs of the catalysis of H2O2on the MP-11/cysteamine/Cu/GCEin 20mM N2 saturated phosphate solution (pH 7.0) in the absence and presence of 0.039mMH2O2. Scan rate: 100mV·s-1.

Table S1 Examples of heme proteins based mediator-free H2O2 biosensors.

Materials / Stability / Linear ranges / Detectionlimit / Refs.
MP-11/Au film / 94% (2 weeks) / 10 µM ~14 mM / 0.4μM / Our work
Fe3O4@ SiO2@hemin / 85% (4 weeks) / 1.0µM~0.16 mM / 2.2×10−4 / [1]
HRP/AuNPs / 83 % (12 weeks) / 8.0µM ~3.0 mM / 2.0μM / [2]
Cyt c/Ag nanocorals / 95% (2 weeks) / 1.8μM ~23 mM / 1.8μM / [3]
Hb/WO3 / 94%(2 weeks) / 3.7µM ~ 0.56 mM / 1.5 μM / [4]
HRP/GNPs-TNTs / 90% (3 days) / 5.0µM ~1.0 mM / 2.1μM / [5]
Mb/ZrO2/chitosan / 90%(2 weeks) / 10.0µM ~1.5 mM / 4.0 μM / [6]
{Hb/CMK-3}n / no change (30days) / 1.2 ~ 57µM / 0.6 μM / [7]
Mb/CDA-[bmim]BF4 / 95%(2 weeks) / 5.0~100 μM / 2.0 μM / [8]
HRP/PTBA PBCs / 90%(1month) / 1 ~ 300 μM / 1 μM / [9]

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