Difference in Environmental Degradability Between Poly(Ethylene Succinate) And

Supporting information

Difference in environmental degradability between poly(ethylene succinate) and poly(3-hydroxybutyrate)

Miwa SUZUKI, Yuya TACHIBANA, Jun-ichiro KAZAHAYA, Reika TAKIZAWA, Fumihiro MUROI, Ken-ichi KASUYA*

Division of Molecular Science, Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin, Kiryu, Gunma 376-8515, Japan

*Corresponding author.

E-mail address: (K-i. Kasuya).

Table S1. Samples aspect

No. / North latitude / East longitude / Sampling site / Sample aspect / Viable microorganism counts（c.f.u./g） / P(3HB)-
degrading microorganism counts (cz.f.u./g) / PESu-
degrading microorganism counts
(cz.f.u./g)
A / 36°28'43.83" / 139°24'50.14" / Gunma, Kiryu / Forest soil / 5.7×106 / 1.2×106 / 2.8×105
B / 36°25'23.99" / 139°20'50.52" / Gunma, Kiryu / University soil / 1.6×106 / 3.0×105 / 1.4×105
C / 36°25'23.99" / 139°20'50.52" / Gunma, Kiryu / University snow / 9.6×103 / 1.6×103 / 6.0×102
D / 36°28'43.83" / 139°24'50.14" / Gunma, Kiryu / Lake water / 2.2×104 / 4.4×103 / 1.4×102
E / 35°41'53.01" / 139°46'26.11" / Tokyo, Chiyoda / City area soil / 3.5×106 / 8.0×105 / 2.2×105
F / 35°38'26.54" / 139°17'36.11" / Tokyo, Hachioji / City area soil / 4.6×106 / 1.1×106 / 2.4×105
G / 35°37'44.78" / 139°44'14.21" / Tokyo, Shinagawa / City area soil / 2.0×107 / 9.3×105 / 1.2×105
H / 35°41'30.70" / 139°46'17.37" / Tokyo, Chiyoda / City area soil / 2.0×107 / 1.0×106 / 1.1×105
I / 35°40'58.44" / 139°45'53.59" / Tokyo, Chiyoda / City area soil / 3.1×107 / 9.3×105 / 6.6×104
J / 35°39'22.35" / 139°45'29.41" / Tokyo, Minato / City area soil / 3.5×107 / 9.4×105 / 1.5×105
K / 35°42'58.03" / 139°46'25.27" / Tokyo, Ueno / Park sand / 1.3×107 / 1.0×106 / 8.6×104
L / 36°20'4.91" / 139°26'22.03" / Tochigi, Ashikaga / River water / 3.3×104 / 8.0×102 / 5.2×101
M / 36°20'4.91" / 139°26'22.03" / Tochigi, Ashikaga / Sludge / 1.3×105 / 3.3×104 / 3.3×103
N / 36°20'4.91" / 139°26'22.03" / Tochigi, Ashikaga / River side soil / 4.8×106 / 1.4×106 / 1.4×105
O / 36°20'19.88" / 139°26'41.33" / Tochigi, Ashikaga / Park sand / 9.3×106 / 1.7×106 / 9.3×104
P / 36°20'19.88" / 139°26'41.33" / Tochigi, Ashikaga / Park soil / 5.3×106 / 1.6×106 / 4.0×104
Q / 36°26'58.03" / 139°22'6.39" / Gunma, Kiryu / Sludge / 7.1×106 / 2.1×106 / 2.3×105
R / 36°26'58.03" / 139°22'6.39" / Gunma, Kiryu / River water / 5.8×104 / 1.0×103 / 1.3×102
S / 36°28'17.87" / 139°24'2.52" / Gunma, Kiryu / Forest soil / 3.3×106 / 1.1×106 / 1.6×105
T / 36°28'27.85" / 139°24'8.50" / Gunma, Kiryu / Forest soil / 3.6×106 / 6.0×105 / 1.1×105
U / 36°28'19.13" / 139°24'1.02" / Gunma, Kiryu / Forest soil / 9.3×106 / 6.0×105 / 1.1×105
V / 35°10'13.56" / 136°52'49.50" / Aichi, Nagoya / City area soil / 2.1×107 / 9.3×105 / 6.0×105
W / 35°10'13.56" / 136°52'49.50" / Aichi, Nagoya / Fountain water / 3.1×105 / 6.0×103 / 9.3×101
X / 36°29'23.58" / 139°24'46.86" / Gunma, Kiryu / River water / 5.4×103 / 8.1×102 / 8.0×102
Y / 36°29'23.58" / 139°24'46.86" / Gunma, Kiryu / River side sand / 4.5×106 / 9.3×105 / 1.5×105
Z / 36°31'55.95" / 139°0'40.01" / Gunma, Shibukawa / Field soil / 1.2×107 / 2.1×106 / 3.1×105
AA / 36°31'55.95" / 139°0'40.01" / Gunma, Shibukawa / River water / 2.3×102 / 5.7×101 / 3.4×10
AB / 36°23'50.26" / 139°17'13.07" / Gunma, Midori / Lake water / 1.1×103 / 1.3×102 / 4.0×10
AC / 36°26'18.11" / 139°10'35.62" / Gunma, Maebashi / Roadside soil / 5.9×106 / 6.7×105 / 1.6×105
AD / 36°23'17.15" / 139°9'11.18" / Gunma, Maebashi / River water / 1.3×103 / 3.3×102 / 5.3×101
AE / 36°23'17.15" / 139°9'11.18" / Gunma, Maebashi / Sludge / 2.9×106 / 6.7×105 / 2.9×105
AF / 36°23'17.82" / 139°9'9.96" / Gunma, Maebashi / Field soil / 2.2×106 / 5.9×105 / 1.1×105
AG / 36°23'40.64" / 139°18'19.90" / Gunma, Midori / Pond water / 3.3×103 / 6.7×102 / 2.3×104
AH / 36°23'37.83" / 139°18'20.47" / Gunma, Midori / Pond water / 3.2×105 / 1.9×103 / 1.6×102
AI / 36°23'37.94" / 139°18'18.44" / Gunma, Midori / Sludge / 1.5×106 / 2.7×105 / 2.5×105
AJ / 34°52'9.84" / 138°19'30.41" / Shizuoka, Yaizu / Coastal seawater / 1.6×104 / 5.4×102 / 0
AK / 35°6'44.58" / 138°35'8.43" / Shizuoka, Kanbara / Coastal seawater / 5.9×103 / 1.4×101 / 0
AK’ / 35°6'44.58" / 138°35'8.43" / Shizuoka, Kanbara / Coastal seawater / 1.3×104 / 2.0×102 / 0
AK’’ / 35°6'44.58" / 138°35'8.43" / Shizuoka, Kanbara / Coastal seawater / 1.7×104 / 4.3×101 / 0
AL / 35°5'53.03" / 139°4'38.06" / Shizuoka, Atami / Coastal seawater / 1.7×102 / 4.8×101 / 0
AL’ / 35°5'53.03" / 139°4'38.06" / Shizuoka, Atami / Coastal seawater / 1.3×102 / 5.7×101 / 0
AM / 36°18'50.19" / 139°26'14.94" / Tochigi, Ashikaga / Field soil / 1.4×106 / 1.2×104 / 4.6×103
AN / 38°30'9.75" / 141°19'7.05" / Miyagi, Ishinomaki / Sludge / 9.8×105 / 1.2×103 / 5.9×102
AO / 36°25'50.83" / 139°19'50.42" / Gunma, Kiryu / Forest soil / 3.6×106 / 1.4×103 / 7.2×102
AP / 36°25'15.06" / 139°20'5.24" / Gunma, Kiryu / Park soil / 6.0×105 / 2.0×103 / 2.2×103
AQ / 36°25'20.62" / 139°21'17.13" / Gunma, Kiryu / Park soil / 1.6×106 / 5.3×103 / 1.4×104
AR / 36°25'19.66" / 139°21'10.02" / Gunma, Kiryu / Field soil / 7.4×106 / 2.2×103 / 1.5×103
AS / 36°25'17.63" / 139°21'7.87" / Gunma, Kiryu / Field soil / 2.1×107 / 6.3×103 / 1.9×104
AT / 36°25'17.63" / 139°21'7.87" / Gunma, Kiryu / Field soil / 2.4×107 / 1.9×104 / 4.8×104
AU / 36°25'18.12" / 139°21'9.37" / Gunma, Kiryu / Field soil / 2.0×105 / 1.6×103 / 2.7×104
AV / 36°25'21.78" / 139°20'50.40" / Gunma, Kiryu / University soil / 8.1×106 / 6.5×104 / 1.6×103
AW / 35°36'2.82" / 139°30'16.93" / Kanagawa, Kawasaki / City area soil / 5.7×106 / 2.3×104 / 1.1×104
AX / 36°15'32.67" / 139°28'36.99" / Gunma, Kiryu / Schoolyard soil / 6.5×105 / 1.3×102 / 2.0×102
AY / 36°27'47.54" / 139°18'34.82" / Gunma, Midori / Field soil / 1.4×106 / 2.0×103 / 2.8×102
AZ / 34°51'18.23" / 135°34'11.77" / Osaka, Takatsuki / Field soil / 4.3×106 / 3.4×104 / 0
BA / 35°10'48.81" / 134°20'7.09" / Okayama, Eida / Roadside soil / 3.8×105 / 2.0×104 / 1.9×102
BB / 36°33'15.95" / 139°10'17.09" / Gunma, Maebashi / Sludge / 1.4×107 / 1.3×103 / 1.9×106
BC / 36°23'39.91" / 139°18'20.38" / Gunma, Midori / Sludge / 1.6×108 / 4.9×103 / 4.4×106
BD / 34°35'59.38" / 135°28'21.11" / Osaka, Sakai / Sludge / 3.3×106 / 7.9×103 / 2.0×103
BE / 34°32'49.65" / 135°30'14.83" / Osaka, Sakai / University soil / 6.5×106 / 2.2×104 / 7.8×103
BF / 34°32'49.65" / 135°30'14.83" / Osaka, Sakai / University soil / 7.0×106 / 8.5×104 / 6.3×103
BG / 34°32'49.65" / 135°30'14.83" / Osaka, Sakai / University soil / 3.2×106 / 1.8×105 / 1.3×104
BH / 34°32'49.65" / 135°30'14.83" / Osaka, Sakai / University soil / 5.5×106 / 4.4×103 / 5.5×102
BI / 34°32'49.65" / 135°30'14.83" / Osaka, Sakai / University soil / 9.3×106 / 1.0×105 / 5.6×103
BJ / 36°23'25.8" / 140°36'52.0" / Ibaraki, Oarai / Coastal seawater / 6.1×102 / 1.5×101 / 0
BK / 37°53'30.5" / 138°58'07.5" / Niigata, Niigata / Coastal seawater / 2.3×102 / 6 / 0
BL / 35°05'23.9" / 139°50'30.2" / Chiba, Minamibouso / Coastal seawater / 9.7×102 / 2.0×101 / 0
BM / 34°59'29.0" / 139°49'29.0" / Chiba, Tateyama / Coastal seawater / 1.6×102 / 2.5×101 / 0

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