Development of On-membrane Analysis for Detection of Gypenosides

Monjuti Sing-ngam1,*,NuttineeSuckho*, SuwannaVorarat# ,WatooPhrompittayarat2,#

1Faculty of Pharmacy, Srinakharinwirot University,Nakhonnayok, 26120, Thailand

2Faculty of Public Health, Naresuan University, Phitsanulok, 65000, Thailand
*email :, ,
#email : ,

Abstract

GynostemmapentaphyllumMakino.,Jiaogulanis known as a medical plant used for lowering blood glucose [1]and stimulating immune[2].Themajor active components of these medications are gypenosides [3]. Owing to the lack of chromophore in the gypenosides structures, HPLC-UV-visible gives low sensitivity for determination of compounds. In the case of the detection of small amounts of gypenosides, on-membrane analysis could be a suitable method [4].Therefore, this study aimsto produce on-membrane analysis using monoclonal antibody (MAb) against gypenosides [5].Gypenosides in the range of 62.5-2,000 ng mL-1 could be detected by on-membrane analysis using anti-gypenosidesMAb. The assay showed a detection limit of 53.07ng.The validated study expressed that the method was sensitive, specific, precise and accurate. The study demomstrated that on-membrane analysis can be used for determination of gypenosides in raw material of Jiaogulan which will be further used for production. This method is simplicity and environmentally friendly.

Acknowledgements:This studywas financially supported by faculty of pharmacy, Srinakharinwirot University, Thailand. The special thanks for kindness of advisor’s team. My major advisor, Dr.WatooPhrompittayarat, who gave good advice and be guidance of this study since start until successful. And my co-advisor, Assoc.Prof.SuwannaVorarat, who is a good guidance for all of comment and good suggestion.

Keyword:Jiaogulan, gypenosides, on-membrane analysis, method validation

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